New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 3330414	51 (1.110)	6 (0.140)	6/270	5.3	10.8%	intergenic (+18/+168)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
	?	NC_000913_3_bme_pgi	= 3330419	51 (1.170)					intergenic (+23/+163)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTG  >  NC_000913_3_bme_pgi/3330272‑3330564                                                                                                                                                     |                                                                                                                                                    aaCGAATGGCGCTTGTGCGATATCGTTCTGGCGATGCAGTAGAACCTGCGGATCAACATAATAGACGTATTCCC‑TTAGAGAGTGTTCAAAGCCGTTTGTACAAATGTCTGTATCAAAACAATTAGTCTAAGAGAAAAATGGGCACATg‑g                                                                                                                                                   >  2:642068/1‑149 (MQ=255)  gccactGGCGGTTTTGCAATATCTTTCTGGCTATGCAGTAGAAACTGCCGATCAGGGTAATGGACATATTTCG‑TTAGTGAATTTCCAAATCCCGTTGTAGCATATGGTTTATCAGAACAATTAGTCAAAAATAAACCCCGGCACATG‑Gc                                                                                                                                                  <  2:171591/144‑1 (MQ=255)          cGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGGACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCtt                                                                                                                                          >  2:510056/1‑149 (MQ=255)                   aaTATCTTTCTGGCTATGCAGTAGAACCGGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAGAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt                                                                                                                                 <  2:183370/149‑1 (MQ=255)                   aaTATCTTTCGGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCGGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt                                                                                                                                 <  2:103951/149‑1 (MQ=255)                         tttCTGGGTATGGATTAGGACCGGGGGAACGGCAGAATGGCAGTAGTCCG‑GTAGTGCGTTTGGAAAGGCGTTGGTATAACGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACaaa                                                                                                                           <  1:45818/149‑1 (MQ=255)                           tCTGGCTATGCAGTAGAACTTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGt                                                                                                                         <  1:162366/149‑1 (MQ=255)                               gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                                                     >  2:186936/1‑149 (MQ=255)                               gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                                                     <  1:734365/149‑1 (MQ=255)                               gCTATGCAGTAGAACCCGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                                                     <  1:189657/149‑1 (MQ=255)                                 tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCtt                                                                                                                   >  2:509085/1‑149 (MQ=255)                                    gCAGTATAACCCGCGGACCAGTGTATCCGCCGTAGTCCG‑TTAGGGCGTTTTGAAAGCCGTTTGTAGAAACATATGTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                <  1:662755/149‑1 (MQ=255)                                    gCAGAAGAACCTGCGGCTCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAAAAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                <  1:760243/149‑1 (MQ=255)                                               tGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGAt                                                                                                     >  1:389565/1‑149 (MQ=255)                                                gCGGATCAGCGTAATCGCCGTATTCCGACCAGT‑TGTTGTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                    >  1:399706/1‑149 (MQ=255)                                                           tAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACg                                                                                         >  2:5141/1‑149 (MQ=255)                                                            aaTCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTAGCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGc                                                                                        >  1:528238/1‑149 (MQ=255)                                                                  cGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                                                  >  1:314187/1‑149 (MQ=255)                                                                    tATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGC‑CATGTGCCggggt                                                                                                                                            >  2:263942/1‑88 (MQ=255)                                                                    tATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGC‑CATGTGCCggggt                                                                                                                                            <  1:263942/89‑2 (MQ=255)                                                                       tCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                             >  1:686895/1‑149 (MQ=255)                                                                                tGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGCCGCGGCGTAAACGCCTTATCCGGCCTACAACAt                                                                     >  2:42042/1‑149 (MQ=255)                                                                                tGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACACAAt                                                                     >  2:403269/1‑149 (MQ=255)                                                                                        gAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCaaa                                                            >  2:744056/1‑149 (MQ=255)                                                                                             ccGTGTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCaa                                                       <  2:643908/149‑1 (MQ=255)                                                                                                     ataAAGATATTTATCAGAACAATTAGTCAAAACGACACCTTGGCACGTG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGACGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGc                                               >  1:193814/1‑149 (MQ=25)                                                                                                                 aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTa                                   >  2:425183/1‑149 (MQ=255)                                                                                                                 aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTa                                   >  2:425228/1‑149 (MQ=255)                                                                                                                     gAACAATTAGCCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGcc                               <  2:658600/149‑1 (MQ=255)                                                                                                                      aaCAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCt                              <  1:378562/149‑1 (MQ=255)                                                                                                                       aCAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTg                             >  2:193156/1‑149 (MQ=255)                                                                                                                       aCAATTACTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑AAAGCGTAGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTg                             <  1:799185/149‑1 (MQ=25)                                                                                                                         aaTTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGAt                           <  1:425183/149‑1 (MQ=255)                                                                                                                         aaTTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGAGGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGAt                           <  1:425228/149‑1 (MQ=255)                                                                                                                           ttAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATaa                         <  1:325561/149‑1 (MQ=255)                                                                                                                           ttAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATaa                         <  2:389565/149‑1 (MQ=255)                                                                                                                           ttAGGCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGAAACAGAGCGCTGTGGTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATaa                         <  1:186936/149‑1 (MQ=25)                                                                                                                                 aaaaaGAACCCCGGTCACAGG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAg                   >  2:300826/1‑149 (MQ=255)                                                                                                                                 aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                <  1:337937/73‑1 (MQ=255)                                                                                                                                 aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                >  2:337937/1‑73 (MQ=255)                                                                                                                                 aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAg                   >  1:159609/1‑149 (MQ=255)                                                                                                                                 aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACACCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCCATTCTCTTGTAGGCCTAATAAACATAg                   >  2:542289/1‑149 (MQ=25)                                                                                                                                 aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGCATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAACTTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAg                   >  2:169174/1‑149 (MQ=255)                                                                                                                                   aaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAgcg                 >  1:227889/1‑149 (MQ=255)                                                                                                                                      gAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt              >  1:436959/1‑149 (MQ=255)                                                                                                                                      gAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt              <  1:5141/149‑1 (MQ=255)                                                                                                                                       agaCCGCGGTACTTG‑GCTGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATc             <  2:55956/147‑1 (MQ=255)                                                                                                                                       aaaCCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATc             <  1:199426/149‑1 (MQ=255)                                                                                                                                          ccccGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAgg          <  1:351826/149‑1 (MQ=255)                                                                                                                                            ccGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt        >  1:325029/1‑149 (MQ=255)                                                                                                                                            ccGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt        <  1:509085/149‑1 (MQ=255)                                                                                                                                            ccGGCACATG‑GATGGGGCTACAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATACTGCAATTCTCTTGTAGGCCTGATAAGCGTATCGCATCAGGCt        <  1:510056/149‑1 (MQ=21)                                                                                                                                              ggCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGa      <  2:227889/149‑1 (MQ=255)                                                                                                                                                 acaTG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAACATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt   >  1:702260/1‑149 (MQ=255)                                                                                                                                                  cacg‑gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAACCGCCCTATCCTGCCTACCCAATCTG‑TCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTg  <  1:394593/146‑1 (MQ=21)                                                                                                                                                     |                                                                                                                                                    AACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCG‑TTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGT‑GCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTG  >  NC_000913_3_bme_pgi/3330272‑3330564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.