| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,296,381:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,296,381 | 1 | . | C | 93.2% | 94.5 / 2.1 | 44 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (12/29); total (13/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913_3_bme_pgi | 4,296,381 | 2 | . | G | 93.2% | 92.5 / 2.1 | 44 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (12/29); total (13/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_bme_pgi/4296296‑4296515 || cGACGCCTGCCGCGTCTTATGAGGCCGAAGCCAGGCAGCGCAATGGCCAGATTGCGGGTGATTTTTTAGGTCGGGCCAGGGGTTT‑‑ACGC‑C‑GCATACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc < 2:369265/149‑1 (MQ=2) aCGCTTAACGCGTCTTATCAGGCCTACCCCAAACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTT‑‑A‑‑CGCAGAATCCGACATCACTCCCCGATGCGACGCTTCCCGCGTCCTCTCCGGCCTCTCTTAAcctc > 2:180259/1‑147 (MQ=1) gCTTGACGCGTCTTATCAGGACTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGCTT‑‑ACGC‑C‑GTATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:758579/149‑1 (MQ=11) gCTTAACGCGTCTTATTATGCTTACGACAGTCCGGCGAACAGCCTGATCTAGCCCGATCTTGTCGGTCTGACACGGCGCTTCGC‑‑C‑C‑GTATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 2:290954/149‑1 (MQ=2) ttAACGCGTCTTATCAGGCCTACGCCAGACAGTGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt < 1:219212/149‑1 (MQ=11) tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt < 1:846817/149‑1 (MQ=255) aCGCGTTTTATCGGGCCTCCGCCAGACACCGTAATAGTCTGATTTAGCGTGATTTTGTAGGTAGGATAAGGCGTTT‑‑CCGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa < 2:261516/149‑1 (MQ=11) cgttctttCAGGCCTACGGCAGACAGCGCAATAGACTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCTACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt < 2:460739/142‑1 (MQ=14) cgTCTTATCTGTCTTACGCCAGACAGCGCCATCGCGTTATTTAGCCTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt < 2:460780/149‑1 (MQ=14) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGTTAATTTTTCAAActg > 2:133326/1‑148 (MQ=255) ggCCTACGCCAGACAGCGCAATACCCTGATTTAGTGTGATTTTTTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct < 1:284554/149‑1 (MQ=17) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 1:265867/149‑1 (MQ=255) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt > 1:476119/1‑149 (MQ=255) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca < 1:841919/149‑1 (MQ=255) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca > 1:598050/1‑149 (MQ=255) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCACTGCCTGATGCGTCGATTGCCGCGTCTTATCAGGCTTATCTTAACCGTTGGTTAATTTTTCAAACTGTTcac > 1:113077/1‑149 (MQ=17) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac < 1:136746/149‑1 (MQ=255) cGCCAGACAGCCCAATAGCCTGATTTAGCGGCATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAAGGCCTGATGCGACGTTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac < 1:297533/149‑1 (MQ=17) cataccgcgcAATAGGCTGTTTGAGCGTGATTTTGTAGGGCGGATAAGGCGTTT‑‑ACGC‑C‑GCACCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg < 2:22258/143‑1 (MQ=17) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca > 1:376805/1‑142 (MQ=255) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg < 1:628668/149‑1 (MQ=255) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACGCGTca < 2:376805/142‑1 (MQ=14) gaaGCGCAATATCTTGATTTTGCGTGATTTATTAGGTCGGAGAAGGCTTTT‑‑ACGC‑C‑GCATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATTAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 2:395713/147‑1 (MQ=18) caaGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGGGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 1:464663/147‑1 (MQ=18) cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc < 1:30765/149‑1 (MQ=255) aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc < 1:131858/149‑1 (MQ=255) attgCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc < 1:91036/146‑1 (MQ=18) gtgCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt < 2:442225/147‑1 (MQ=255) gCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCGCACCCGGTATCAACCCCttg > 2:180435/1‑148 (MQ=18) gCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGTCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCGGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:798753/149‑1 (MQ=21) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc > 2:582648/1‑149 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACTCTTGCTGCGTCTTATCAGGCCTTTCTTATCCGTTGATTAATTTTTCAAACTCTTCATACCCGGTCTAAAAACCTTCCa > 1:628029/1‑149 (MQ=17) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 1:630505/149‑1 (MQ=255) tGATTTTGTAGGTCGGAT‑AGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt > 2:641263/1‑149 (MQ=25) ttttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCTTAACCGTTGGTTAATTTATCAAACACTTCACACCCGGTATCAAACCCTTACATACAGATCAGGTTc > 1:285737/1‑149 (MQ=21) tttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAACCGTTGGTTAATTTTTAAAACTCTTCACACCCGATATCAAAACCTTACATACCTCTCAGGTTCa > 1:124725/1‑149 (MQ=21) ttGTAGGTCGGATAAGGCATTG‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTAGTTAATTTGTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa < 2:324431/149‑1 (MQ=25) tctAGGTCGGATAAGTCGTTT‑‑ACGC‑C‑GTATCCGACATCAATGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 2:634003/147‑1 (MQ=25) tAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATTCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa < 1:370016/149‑1 (MQ=255) gaagTCGGATAAGGCGTTT‑‑ACGC‑C‑GTATCCGCAATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa < 2:221408/146‑1 (MQ=25) gTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCGCAGGTTTCAAACCAATCCATACAGCACAAGTTCAAcaactg > 1:387835/1‑144 (MQ=18) gTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTg < 1:286604/149‑1 (MQ=255) gaaaaaGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACACTTGCCCCGTCTTATCAGGCCTATCTTAACCGTTGTTTAATTATTCAAACGCCGCACACCCAGTACCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg < 2:410820/145‑1 (MQ=21) gATAAGGCGTTT‑‑ACGC‑C‑GCATCCGAGATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGGCTATCTTAACCGTTGGCTAATTTTTCAAACTCTTCACGCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc < 1:812657/149‑1 (MQ=25) || CGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC > NC_000913_3_bme_pgi/4296296‑4296515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |