Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	4,235,435	Δ1 bp	intergenic (‑2202/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	4,235,433	0	A	.	100.0%	381.8 / NA	87	intergenic (‑2200/‑501)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (46/41);  total (46/41)

CCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_hsa_pgi/4235290‑4235577                                                                                                                                                |                                                                                                                                                 ccAGTGGGGAGTTGAGCTGGGAAAGCAGCAGGCTAAGAAAATAGAGCCTGAGCTTGATCGAAGTGCTCAAGTGACCTCTCACGACGCATCTACAAATGGGCTCATCAACATCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATaatcat                                                                                                                                             >  1:1024545/1‑145 (MQ=255)     tGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCAtcgtc                                                                                                                                        >  2:186006/1‑149 (MQ=255)       gggAGTTGAGCTGCTTAAGCATGTGGGTAACAAAATAGCTCCTGAGCTTTATGGATGTGATCAAGTTACATCTCACGACGCTTATACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑ATTCATCGTCGa                                                                                                                                      <  2:327010/149‑1 (MQ=255)       gggAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGCGCTTGATGTCAGTGCTCAAGTGACCTCTCACGACTCTTCTACCAATGGTCTCTTCAACTTAATCAACGAGCAGCGCGAGTCCAGAGTCCAAT‑Aatcatccacga                                                                                                                                      >  1:804523/1‑144 (MQ=255)           gTGGAGCGGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATg                                                                                                                                  <  1:33958/149‑1 (MQ=255)               aGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAgg                                                                                                                              >  1:846831/1‑149 (MQ=255)                 cTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGcc                                                                                                                            >  1:327072/1‑149 (MQ=255)                  tGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCg                                                                                                                           <  2:541240/149‑1 (MQ=255)                   gggAAAGCAGCTTGCTAAGAAAATATAGACTGAGCTTGATGGCAGTGATCAAGTGACCTCTCACGACGCTTATACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCgg                                                                                                                          <  1:845036/149‑1 (MQ=255)                    ggAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGCCGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATAAGTAGGCCGGa                                                                                                                         >  1:1234615/1‑149 (MQ=255)                    ggAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGGCGATATGTAGGCCGGa                                                                                                                         >  1:1234610/1‑149 (MQ=255)                     gAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCACCTTCATCAAGCAGCAGCGCGCGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGCCCGGAt                                                                                                                        >  1:933410/1‑149 (MQ=255)                      aaaGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATa                                                                                                                       <  1:180762/149‑1 (MQ=255)                        agcagcTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     <  1:439826/149‑1 (MQ=255)                          aaggtgtcTAATAAAATAGAGACTGAGCTTGATGGCAGTGCTCTAGTGACCTCTCACGAAGCTTCTACAAATTGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGGCCAAT‑AAGCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   <  1:732568/142‑1 (MQ=255)                                gCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                                                                             >  1:1091948/1‑149 (MQ=255)                                gCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                                                                             <  2:1202521/149‑1 (MQ=255)                                 ctttgAATATATAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGAGGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGTGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                            <  1:80252/145‑1 (MQ=255)                                  tAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           <  1:772076/149‑1 (MQ=255)                                     gAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGTCCGGATAAGGCGTTCAcgccgc                                                                                                        >  2:885608/1‑149 (MQ=255)                                        aaTAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                                                     <  2:421719/149‑6 (MQ=255)                                        aaTAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                     >  1:647887/1‑149 (MQ=255)                                         aTAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                    >  2:709892/1‑149 (MQ=255)                                             agCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGAAATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                <  1:927533/149‑1 (MQ=255)                                                   cgCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCACTGGGCTCATCAACTTCATCACGCAGCACCGCGAGGCCAGAGTCCAAT‑AATCCTCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                          <  1:11892/148‑1 (MQ=39)                                                      ttGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                       >  2:501634/1‑149 (MQ=255)                                                               ttGCTCAATTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                              <  2:944661/148‑1 (MQ=255)                                                                tGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                             <  1:709892/149‑1 (MQ=255)                                                                  cTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                           <  1:186006/149‑1 (MQ=255)                                                                   tCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCACCTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                                                                          >  2:1051881/1‑149 (MQ=255)                                                                    cAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCGAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                         <  1:145392/149‑1 (MQ=255)                                                                     aaGTGACCTCTCACGACGCTTCTACCATTGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGACGCATCCGGCCACCGATGCCTGATGCGACGCGGtg                                                                        >  2:197544/1‑148 (MQ=39)                                                                     aaGAGACCTCTCACTACGGTTCTACCAATGGGCTCATCAACTTCATCATGCAGCAGCGCGAGGCCATAGTCCAAT‑AATCTTCGTCGATATGTAGGCGGGATAAGGCGTTCTGGCCGCATCCGGCTACCGATGCCTGATGCGACGCGGTc                                                                        <  1:211126/149‑1 (MQ=255)                                                                       gTGCCCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      <  2:272736/149‑1 (MQ=255)                                                                       gTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAAGTTCATCAAGCAGCAGCGAGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAGAGATGCCTGATGCGAAGCGGTctc                                                                      >  2:119007/1‑147 (MQ=38)                                                                       gTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGgcgc                                                                      >  2:632564/1‑149 (MQ=255)                                                                       gTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                      <  2:119711/149‑1 (MQ=255)                                                                             tctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCttt                                                                >  2:797758/1‑148 (MQ=255)                                                                              ctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               >  2:905154/1‑149 (MQ=255)                                                                              ctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               <  1:1250162/149‑1 (MQ=255)                                                                              ctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               <  2:374243/149‑1 (MQ=255)                                                                              ctcACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                               >  2:395327/1‑149 (MQ=255)                                                                              ctcACGACGCTTCTACCAATGGGCTCATCAACTTCAACAGGCAGCAGCGCGCGGCCAGAGTCAAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAACCGATGCCTGAGGCGCCGGGGGCGCGTCTTag                                                               >  2:966610/1‑148 (MQ=37)                                                                                cACGAAGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGTGGCCAGAGTCCAAT‑AATCATCTGCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGTCTGATAAGACGCGACCGCGTCGCATCa                                                             <  2:804523/149‑1 (MQ=37)                                                                                  cgacgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCACCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAgg                                                           <  1:3683/149‑1 (MQ=38)                                                                                   gacgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                          <  2:628099/149‑1 (MQ=255)                                                                                    ccgCTGCTACCCTTGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                         <  2:1047094/148‑1 (MQ=37)                                                                                    acgCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                         <  2:405593/149‑1 (MQ=255)                                                                                       cTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                      <  2:1091948/149‑1 (MQ=255)                                                                                        ttCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                     >  1:518545/1‑149 (MQ=255)                                                                                            aCCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCCCAAACGGCAACCGATCCCTGATCCGACGCGGTCGCGTCTAATCAGGCCTACAGGTc                                                 >  1:133607/1‑149 (MQ=38)                                                                                             ccAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                >  2:459690/1‑149 (MQ=255)                                                                                             ccAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGAGATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCATGATGCGAAGAGGTGGCGTCTAATGACGCCTACAGGTcc                                                >  2:1049299/1‑148 (MQ=37)                                                                                             ccAATGGGCTCATCAACTTCATCAAGCAGAAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                >  2:216630/1‑149 (MQ=255)                                                                                             ccAATGGGCTCATCAACGTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCAGCAGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTTCAGGTCg                                                >  2:1137686/1‑149 (MQ=37)                                                                                              cAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                               >  2:868652/1‑149 (MQ=255)                                                                                              cAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                               >  2:145719/1‑149 (MQ=255)                                                                                              cAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTACAAT‑CATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCATTATGCGACCCGGTCGAGTCATATAAGGCCAACAGGTCgc                                               >  1:693334/1‑148 (MQ=37)                                                                                                aTGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg                                             >  2:996566/1‑149 (MQ=255)                                                                                                aTGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGGCGATg                                             >  2:830671/1‑149 (MQ=255)                                                                                                aTGGGCGCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCACT‑CATCATCGTCGATCTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg                                             <  2:327072/149‑1 (MQ=25)                                                                                                 tGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                            <  2:828627/149‑1 (MQ=255)                                                                                                   ggCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                          <  1:1232607/149‑1 (MQ=255)                                                                                                    gCTCCTCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATCTGTAGGCCGGAGAAGGCGTTCACGCCGCATCCGGCATCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                         <  1:217559/149‑1 (MQ=37)                                                                                                    gCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTGGTATCCGGCCTACAGGTAGAtaataa                                         >  2:1161320/1‑144 (MQ=37)                                                                                                     cTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                        >  1:876480/1‑149 (MQ=255)                                                                                                      tcatcaACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                       >  1:1010521/1‑149 (MQ=255)                                                                                                      tcatcaACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                       >  1:167561/1‑149 (MQ=255)                                                                                                          caACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCACCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTa                                   >  1:898354/1‑149 (MQ=255)                                                                                                              ttCATCAAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                               >  2:1177013/1‑149 (MQ=255)                                                                                                                 atcaAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                            >  2:1245101/1‑149 (MQ=255)                                                                                                                  tcaAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAACCGATGCCTTATGCGACGCGGTCGCGTCGAATCATGCATACAGGTCGATCCCGATATGTCAATCGTAt                           >  1:1108546/1‑149 (MQ=25)                                                                                                                   caAGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                          <  1:501634/149‑1 (MQ=255)                                                                                                                    aaGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCCTCGTCTATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                         <  2:933410/149‑1 (MQ=255)                                                                                                                    aaGCAGCAGCGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCATATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                         >  1:302870/1‑149 (MQ=255)                                                                                                                     agcagcagcGCGCGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCACCCGATGCCTGATGCGACGCCGGCGCGTCGTAGCAGGGGTAGAGGTCGATGGCGATCTGTACATCGGATTCg                        >  2:590122/1‑149 (MQ=25)                                                                                                                         gcagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGGCGATGCCGATATGTACATCGTATTCGGCaa                    >  1:534790/1‑149 (MQ=255)                                                                                                                          cagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                   >  1:234962/1‑149 (MQ=255)                                                                                                                          cagcGCGAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                   >  1:105950/1‑149 (MQ=255)                                                                                                                              gcgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt               <  2:1235192/149‑1 (MQ=255)                                                                                                                               cgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa              >  1:1011121/1‑149 (MQ=255)                                                                                                                               cgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa              <  1:996566/149‑1 (MQ=255)                                                                                                                               cgAGGCCAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa              <  2:336999/149‑1 (MQ=255)                                                                                                                                    ccAGAGTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg         >  1:372329/1‑149 (MQ=255)                                                                                                                                      agagTCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa       <  2:71231/149‑1 (MQ=255)                                                                                                                                          tCCAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCCATAAGTACATCGTATTCGGCAATAAAAACATAGCACgat   >  1:324388/1‑149 (MQ=25)                                                                                                                                           ccAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  2:167561/149‑1 (MQ=255)                                                                                                                                           ccAAT‑AATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  1:830671/149‑1 (MQ=255)                                                                                                                                           ccAAT‑AATCATCGCCGATAGGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCAACAGATGCCTGATGCGACGCGGTAGCGTCTTATCAGTCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  <  2:700722/149‑1 (MQ=37)                                                                                                                                                |                                                                                                                                                 CCAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_hsa_pgi/4235290‑4235577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑