Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	4,015,910	Δ1 bp	coding (260/816 nt)	ysgA ←	putative carboxymethylenebutenolidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	4,015,910	0	A	.	100.0%	435.4 / NA	89	coding (260/816 nt)	ysgA	putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (42/47);  total (42/47)

CCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCC  >  NC_000913_3_KO_pgi/4015766‑4016053                                                                                                                                                 |                                                                                                                                                ccgTCGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCa                                                                                                                                           <  2:304182/150‑1 (MQ=255)  cgTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAg                                                                                                                                          <  3:263909/150‑1 (MQ=255)   gTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGa                                                                                                                                         <  1:357588/150‑1 (MQ=255)    ttGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGAt                                                                                                                                        >  4:76464/1‑150 (MQ=255)    ttGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGAt                                                                                                                                        <  3:76464/150‑1 (MQ=255)      gCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAc                                                                                                                                      <  1:49274/150‑1 (MQ=255)         ggAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGCAAAGTTCAGGTGCG‑TAGCCAGATAcccc                                                                                                                                   >  4:48700/1‑150 (MQ=255)               ccAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgc                                                                                                                             >  2:53309/1‑150 (MQ=255)                 aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                           <  2:753441/150‑1 (MQ=255)                 aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                           >  2:399094/1‑150 (MQ=255)                 aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                           >  3:232456/1‑150 (MQ=255)                 aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGGCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                           >  2:361050/1‑150 (MQ=255)                  aCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGcc                                                                                                                          <  2:719823/150‑1 (MQ=255)                        gACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATCCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACgg                                                                                                                    >  1:281436/1‑150 (MQ=255)                         attaaaTCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGc                                                                                                                   <  2:286530/145‑1 (MQ=255)                         aCATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGc                                                                                                                   <  2:711252/150‑1 (MQ=255)                           aTGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGa                                                                                                                 <  1:53309/150‑1 (MQ=255)                           aTGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGa                                                                                                                 <  4:232456/150‑1 (MQ=255)                              aTCGAGATCGGCCAGCACCTGCGAGTCNGGCACTTTTGCTACNAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCCGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACaa                                                                                                              >  1:680116/1‑150 (MQ=255)                                   gaTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCATATACCCCTCCAGCGCCAGACGGCGACAAATGTc                                                                                                         <  2:418997/150‑1 (MQ=255)                                         ccAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGat                                                                                                   >  1:382136/1‑150 (MQ=255)                                          cAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACa                                                                                                               <  3:279961/137‑1 (MQ=255)                                          cAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACa                                                                                                               >  4:279961/1‑137 (MQ=255)                                                cTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCa                                                                                            <  1:76370/150‑1 (MQ=255)                                                 tGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATAACGGCAAAACCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAt                                                                                           <  3:232825/150‑1 (MQ=255)                                                     aGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCAc                                                                                       <  1:106375/150‑1 (MQ=255)                                                         aGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCa                                                                                   >  4:165611/1‑150 (MQ=255)                                                          ggCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCaa                                                                                  >  2:453703/1‑150 (MQ=255)                                                          ggCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGACaa                                                                                  >  1:740913/1‑150 (MQ=255)                                                           gCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCaaa                                                                                 >  1:586093/1‑150 (MQ=255)                                                                tttGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAAttt                                                                            >  2:763281/1‑150 (MQ=255)                                                                 ttGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCTAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                           <  2:517613/150‑1 (MQ=255)                                                                 ttGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                           <  1:398487/150‑1 (MQ=255)                                                                    cTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTg                                                                        <  4:173960/150‑1 (MQ=255)                                                                      aCCAGACCGCTAAGCAACGTGGGGATATCGGCAGAATCCTTCGGATCGCCATCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCAAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCa                                                                      >  4:192751/1‑150 (MQ=255)                                                                       ccAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCAc                                                                     >  3:452854/1‑150 (MQ=255)                                                                        cAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACt                                                                    >  1:238525/1‑150 (MQ=255)                                                                        cAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACt                                                                    >  4:375876/1‑150 (MQ=255)                                                                         aGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTa                                                                   >  1:782159/1‑150 (MQ=255)                                                                          gACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTAc                                                                  >  4:234641/1‑150 (MQ=255)                                                                              gCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATg                                                              >  3:53117/1‑150 (MQ=255)                                                                              gCTAAGCAACGGGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATg                                                              <  2:268295/150‑1 (MQ=255)                                                                               cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGa                                                                                                    <  1:282506/111‑1 (MQ=255)                                                                               cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGa                                                                                                    >  2:282506/1‑111 (MQ=255)                                                                                tAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGAc                                                            >  4:517344/1‑150 (MQ=255)                                                                                 aaGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGAcc                                                           >  2:187409/1‑150 (MQ=255)                                                                                     aaCGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTg                                                       <  3:234641/150‑1 (MQ=255)                                                                                        gTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCa                                                    <  1:187409/150‑1 (MQ=255)                                                                                        gTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCa                                                    >  4:453374/1‑150 (MQ=255)                                                                                          ggggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGt                                                  >  2:178759/1‑150 (MQ=255)                                                                                           gggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACt                                                        >  2:74101/1‑143 (MQ=255)                                                                                           gggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACt                                                        <  1:74101/143‑1 (MQ=255)                                                                                            ggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCCCGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTgg                                                <  1:404301/150‑1 (MQ=255)                                                                                            ggATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTgg                                                <  3:165611/150‑1 (MQ=255)                                                                                                atCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCa                                            >  2:422099/1‑150 (MQ=255)                                                                                                atCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCa                                            >  2:422174/1‑150 (MQ=255)                                                                                                 tCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGGCGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCAt                                           >  3:68865/1‑150 (MQ=255)                                                                                                  cGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTc                                                                                             <  1:247075/99‑1 (MQ=255)                                                                                                  cGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTc                                                                                             >  2:247075/1‑99 (MQ=255)                                                                                                    gCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgc                                        >  4:166711/1‑150 (MQ=255)                                                                                                      aaaaTCATTCGGATCGCCGTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctt                                      <  2:511285/150‑1 (MQ=255)                                                                                                      aaaaTCATCCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctt                                      <  2:281436/150‑1 (MQ=255)                                                                                                         aTCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAt                                                                                           <  4:254224/94‑1 (MQ=255)                                                                                                         aTCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAt                                                                                           >  3:254224/1‑94 (MQ=255)                                                                                                          tCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgct                                  >  2:81004/1‑150 (MQ=255)                                                                                                            aTTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgcttt                                <  1:361050/150‑1 (MQ=255)                                                                                                            aTTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgcttt                                <  1:81004/150‑1 (MQ=255)                                                                                                             ttCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGctttgctttg                               >  1:66765/1‑150 (MQ=255)                                                                                                               cGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGt                             <  4:117612/150‑1 (MQ=255)                                                                                                                ggATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTc                            >  4:238684/1‑150 (MQ=255)                                                                                                                 gATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGa                                                             >  4:110623/1‑116 (MQ=255)                                                                                                                 gATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGa                                                             <  3:110623/116‑1 (MQ=255)                                                                                                                  aTCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTg                          <  4:452854/150‑1 (MQ=255)                                                                                                                  aTCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTg                          <  1:73161/150‑1 (MQ=255)                                                                                                                   tCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTgg                         <  1:215026/150‑1 (MQ=255)                                                                                                                   tCGCCTCCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCTCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTgg                         <  1:447217/150‑1 (MQ=255)                                                                                                                      ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCAt                      >  1:137654/1‑150 (MQ=255)                                                                                                                       cTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATg                     <  2:822993/150‑1 (MQ=255)                                                                                                                          cgcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATATTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTa                  >  1:771418/1‑150 (MQ=255)                                                                                                                           gcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGGCGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTaa                 <  4:68865/150‑1 (MQ=255)                                                                                                                             ggAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGc               >  3:170827/1‑150 (MQ=255)                                                                                                                             ggAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGc               <  1:763281/150‑1 (MQ=255)                                                                                                                                 gTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGc           <  2:782159/150‑1 (MQ=255)                                                                                                                                 gTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTACTTTGGTCTGGCATGGTAAGCAGGc           <  2:382136/150‑1 (MQ=255)                                                                                                                                   aaaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCAt         <  4:299428/150‑1 (MQ=255)                                                                                                                                    aaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATg        <  1:422099/150‑1 (MQ=255)                                                                                                                                    aaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATg        <  1:422174/150‑1 (MQ=255)                                                                                                                                          aGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATcc  <  3:517344/150‑1 (MQ=255)                                                                                                                                          aGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATcc  >  1:724797/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                CCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCC  >  NC_000913_3_KO_pgi/4015766‑4016053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑