BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	4,015,910	Δ1 bp	coding (260/816 nt)	ysgA ←	putative carboxymethylenebutenolidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	4,015,910	0	A	.	100.0%	896.6 / NA	179	coding (260/816 nt)	ysgA	putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (86/93); total (86/93)

Only 100 of 179 total aligned reads displayed.

CCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCC  >  NC_000913_3_KO_pgi/4015766‑4016054

  gTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTTGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGa                                                                                                                                          <  1:877068/150‑1 (MQ=255)

     gCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAc                                                                                                                                       <  3:693132/150‑1 (MQ=255)

       gggAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAccc                                                                                                                                     <  2:186527/150‑1 (MQ=255)

       gggAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAccc                                                                                                                                     <  4:18640/150‑1 (MQ=255)

       gggAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAccc                                                                                                                                     <  1:1600807/150‑1 (MQ=255)

          gaGCCCAGCGGGCGACATGATCGAGATCGGCCAGCACCTGCGAGGCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTc                                                                                                                                  <  4:845770/149‑1 (MQ=255)

             cccAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAg                                                                                                                               >  3:998275/1‑150 (MQ=255)

             cccAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAg                                                                                                                               >  2:1044406/1‑150 (MQ=255)

               cAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcg                                                                                                                             >  4:484649/1‑150 (MQ=255)

               cAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcc                                                                                                                             >  2:923658/1‑149 (MQ=255)

                aaCTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAgcgc                                                                                                                            >  2:1025011/1‑150 (MQ=255)

                  cTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCa                                                                                                                          >  4:590045/1‑150 (MQ=255)

                     gCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGAc                                                                                                                       >  4:626412/1‑150 (MQ=255)

                     gCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGAc                                                                                                                       >  1:991440/1‑150 (MQ=255)

                       gACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACgg                                                                                                                     >  1:1401132/1‑150 (MQ=255)

                       gACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACgg                                                                                                                     <  1:347224/150‑1 (MQ=255)

                        aCATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGc                                                                                                                    <  2:1000413/150‑1 (MQ=255)

                             aTCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACat                                                                                                               >  2:1065889/1‑149 (MQ=255)

                             aTCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACaa                                                                                                               >  2:882730/1‑150 (MQ=255)

                               cGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAAt                                                                                                             >  2:785331/1‑150 (MQ=255)

                                 agaTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGGAAAGTTCAGGTGCG‑TAGCCAGATACACCACCAGCGCCAGCCGGCGACAAATGt                                                                                                           >  4:44880/1‑150 (MQ=255)

                                   aTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcg                                                                                                         <  2:593363/150‑1 (MQ=255)

                                   aTCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcg                                                                                                         <  2:497529/150‑1 (MQ=255)

                                    tCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgc                                                                                                        <  1:451065/150‑1 (MQ=255)

                                    tCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgc                                                                                                        >  4:79651/1‑150 (MQ=255)

                                     cGGCCAGCACCTGGATCTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgcg                                                                                                       <  1:1361601/150‑1 (MQ=255)

                                      ggCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCACCAGACGGCGACAAATGTCGCgg                                                                                                      >  2:498350/1‑150 (MQ=255)

                                       gCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGa                                                                                                     <  3:253458/150‑1 (MQ=255)

                                        ccAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGat                                                                                                    <  1:1673911/150‑1 (MQ=255)

                                         cAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAg                                                                                                                                           <  1:1014825/110‑1 (MQ=255)

                                          aGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTGGTATCGGCTCCGCTGAAGGGAGGGGCAGGGTCG‑TAGCCGGTTACCCCTCCAGCGCCAGACGGCGACAAAGGTCGCGGatat                                                                                                  >  3:991313/1‑150 (MQ=255)

                                            cACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGt                                                                                                <  3:719289/150‑1 (MQ=255)

                                              ccTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTc                                                                                              <  1:830621/150‑1 (MQ=255)

                                              ccTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTc                                                                                              <  2:1223933/150‑1 (MQ=255)

                                              ccTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTc                                                                                              <  1:434445/150‑1 (MQ=255)

                                               cTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCa                                                                                             >  1:566835/1‑150 (MQ=255)

                                                tGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAt                                                                                            <  4:777839/150‑1 (MQ=255)

                                                 gCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATg                                                                                           >  4:58775/1‑150 (MQ=255)

                                                  cGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGc                                                                                          >  1:6898/1‑150 (MQ=255)

                                                  cGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGc                                                                                          <  2:1259587/150‑1 (MQ=255)

                                                    aGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTcgcg                                                                                                       >  1:513374/1‑135 (MQ=255)

                                                         ggCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCaa                                                                                   >  4:919973/1‑150 (MQ=255)

                                                                ttGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                            <  1:1164334/150‑1 (MQ=255)

                                                                 tGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTcc                                                                           <  3:850597/150‑1 (MQ=255)

                                                                 tGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTcc                                                                           <  1:1025011/150‑1 (MQ=255)

                                                                  gCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCt                                                                          >  1:1081571/1‑150 (MQ=255)

                                                                   cTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCta                                                                         >  4:732731/1‑149 (MQ=255)

                                                                     aCCAGACCGCTAAGCAACGTGGGGATATCGGCACAATCATTCGGATCGCCTTCGCGGAGGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCa                                                                       >  2:943628/1‑150 (MQ=255)

                                                                     aCCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAAtt                                                                              >  2:998553/1‑143 (MQ=255)

                                                                      ccAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCAc                                                                      >  2:1433753/1‑150 (MQ=255)

                                                                        aGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTa                                                                    <  3:609401/150‑1 (MQ=255)

                                                                          aCCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACa                                                                  <  1:1044406/150‑1 (MQ=255)

                                                                            cGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAAt                                                                >  1:464939/1‑150 (MQ=255)

                                                                            cGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAAt                                                                >  3:434217/1‑150 (MQ=255)

                                                                            cGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAAAAAAAAAAAAAATTTCCTGCACTACAAt                                                                <  2:991440/150‑1 (MQ=255)

                                                                              cTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGa                                                              >  2:251636/1‑150 (MQ=255)

                                                                               tAAGCTACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGAc                                                             <  4:847442/150‑1 (MQ=255)

                                                                               tAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGAc                                                             <  2:1559428/150‑1 (MQ=255)

                                                                                     aCGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTgg                                                       <  1:1157857/150‑1 (MQ=255)

                                                                                      cGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGc                                                      >  2:1653950/1‑150 (MQ=255)

                                                                                            gATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTggg                                                >  2:299312/1‑150 (MQ=255)

                                                                                              tatCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCAt                                                                                            >  3:336036/1‑104 (MQ=255)

                                                                                                  ggCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCg                                          >  4:701928/1‑150 (MQ=255)

                                                                                                   gCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGa                                                              >  1:1330240/1‑129 (MQ=255)

                                                                                                     aaaaTCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctt                                       <  4:679304/150‑1 (MQ=255)

                                                                                                      aaaTCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgcttt                                      <  3:189105/150‑1 (MQ=255)

                                                                                                           aTTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGACAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgctttgcttt                                 <  2:328137/150‑1 (MQ=255)

                                                                                                            ttCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGctttgctttg                                >  4:518872/1‑150 (MQ=255)

                                                                                                            ttCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGctttgctttg                                <  1:299312/150‑1 (MQ=255)

                                                                                                            ttCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGctttgctttg                                <  2:765737/150‑1 (MQ=255)

                                                                                                             tCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATAc                                                                                                                                       <  3:516841/46‑1 (MQ=255)

                                                                                                               ggATCGCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTc                             <  2:1338663/150‑1 (MQ=255)

                                                                                                               ggATCGCCTTCGCGGAAGTAAAATTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTc                             >  1:1491714/1‑150 (MQ=255)

                                                                                                                    gCCTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCgcttt                                      <  4:317473/136‑1 (MQ=255)

                                                                                                                     ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCAc                                                                                        >  1:784261/1‑85 (MQ=255)

                                                                                                                     ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCAt                       <  1:882730/150‑1 (MQ=255)

                                                                                                                     ccTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCAt                       >  4:626547/1‑150 (MQ=255)

                                                                                                                      cTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATg                      <  3:44880/150‑1 (MQ=255)

                                                                                                                      cTTCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATg                      <  2:464939/150‑1 (MQ=255)

                                                                                                                      cTTCGCGGAAGTAAAATTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATg                      <  2:1491714/150‑1 (MQ=255)

                                                                                                                       ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTc                                                                            >  2:1682329/1‑95 (MQ=255)

                                                                                                                       ttCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATgg                     <  2:82218/150‑1 (MQ=255)

                                                                                                                        tCGCGGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACCGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGt                    <  1:1662296/150‑1 (MQ=255)

                                                                                                                          gcgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTaa                  >  4:366530/1‑150 (MQ=255)

                                                                                                                           cgGAAGTAAAGTTCAGTTGCG‑TAGCAAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAg                 >  2:859191/1‑150 (MQ=255)

                                                                                                                           cgGAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAg                 <  1:1252990/150‑1 (MQ=255)

                                                                                                                            ggAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTCCAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAgg                >  1:553280/1‑149 (MQ=255)

                                                                                                                            ggAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGc                >  2:1591709/1‑150 (MQ=255)

                                                                                                                            ggAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGc                >  3:650904/1‑150 (MQ=255)

                                                                                                                             gAAGTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCa               >  1:143079/1‑150 (MQ=255)

                                                                                                                              aaGTAAAGTTCAGGTGCG‑TAGCCAGATGCCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAg              <  2:414163/150‑1 (MQ=255)

                                                                                                                                gTAAAGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGc            >  1:133716/1‑150 (MQ=255)

                                                                                                                                   aaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATg         >  1:1684154/1‑150 (MQ=255)

                                                                                                                                   aaGTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATg         <  2:566835/150‑1 (MQ=255)

                                                                                                                                     gTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGtt       >  3:788297/1‑150 (MQ=255)

                                                                                                                                     gTTCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGCCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGtt       <  3:58775/150‑1 (MQ=255)

                                                                                                                                       tCAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTAt     <  2:1336017/150‑1 (MQ=255)

                                                                                                                                        cAGGTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATc    <  3:252801/150‑1 (MQ=255)

                                                                                                                                          ggTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATccc  <  3:817396/150‑1 (MQ=255)

                                                                                                                                          ggTGCG‑TAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATccc  <  1:943628/150‑1 (MQ=255)

CCGTTGCGGGAAGCCCAACTGGCGACATGATCGAGATCGGCCAGCACCTGCGAGTCAGGCACTTTTGCTACCAGACCGCTAAGCAACGTGGGGATATCGGCAAAATCATTCGGATCGCCTTCGCGGAAGTAAAGTTCAGGTGCGATAGCCAGATACCCCTCCAGCGCCAGACGGCGACAAATGTCGCGGATATGTTCATGCACGCCAAAAATTTCCTGCACTACAATGACCACTGGCAGTGGGCCATCGCTTTGCTTTGGTCTGGCATGGTAAGCAGGCATGTTATCCC  >  NC_000913_3_KO_pgi/4015766‑4016054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`