New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 2643100	67 (1.160)	13 (0.250)	12/228	4.2	18.9%	intergenic (‑256/+29)	rodZ/rlmN	MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
	?	NC_000913_3_KO_pgi	= 2643110	52 (1.010)					intergenic (‑266/+19)	rodZ/rlmN	MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCAC  >  NC_000913_3_KO_pgi/2642966‑2643257                                                                                                                                                  |                                                                                                                                                    aCAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGt                                                                                                                                                >  1:403190/1‑150 (MQ=255)  acgagcacacaTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTg                                                                                                                                               <  1:113408/143‑1 (MQ=255)    aaGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGc                                                                                                                                             <  1:626860/150‑1 (MQ=255)       cccACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCACCTTAATACTTAATTGATAAAAGATTACGAAACGG‑TACATAAAGTAACCGTGGCGTa                                                                                                                                          >  1:482607/1‑150 (MQ=255)        ccACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTaa                                                                                                                                         >  3:299992/1‑150 (MQ=255)        ccACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTaa                                                                                                                                         >  2:335108/1‑150 (MQ=255)         cacaTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAAt                                                                                                                                        <  1:510072/150‑1 (MQ=255)            aTTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGc                                                                                                                                     >  4:44938/1‑150 (MQ=255)                  tAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAg                                                                                                                               >  4:123728/1‑150 (MQ=255)                         aaCTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCtt                                                                                                                        >  2:31795/1‑150 (MQ=255)                           cTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTa                                                                                                                      <  1:647770/150‑1 (MQ=255)                           cTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTa                                                                                                                      >  3:203065/1‑150 (MQ=255)                                tGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTc                                                                                                                 <  2:39718/150‑1 (MQ=255)                                   tGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGAt                                                                                                              <  2:370711/150‑1 (MQ=255)                                     cATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCACCTTAATACTTAATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATgg                                                                                                            <  3:84180/150‑1 (MQ=255)                                     cATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGT‑ACCGTGGCGTAATgttt                                                                                                                                    <  2:402327/126‑4 (MQ=37)                                     cATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGT‑ACCGTGGCGTAATgttt                                                                                                                                    >  1:402327/1‑123 (MQ=37)                                     cATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATgg                                                                                                            >  1:217648/1‑150 (MQ=255)                                      aTCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAAt                                                                                                                                        <  4:43514/121‑1 (MQ=255)                                      aTCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAAt                                                                                                                                        >  3:43514/1‑121 (MQ=255)                                          ttACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCa                                                                                                       >  1:411249/1‑150 (MQ=255)                                           tACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAc                                                                                                      <  2:217648/150‑1 (MQ=255)                                            aCATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAcc                                                                                                     >  4:343589/1‑150 (MQ=255)                                             cATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAccc                                                                                                    >  1:674774/1‑150 (MQ=255)                                              aTACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCt                                                                                                   >  3:374200/1‑150 (MQ=255)                                                 cTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGa                                                                                                                              >  3:406157/1‑120 (MQ=255)                                                 cTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGa                                                                                                                              <  4:406157/120‑1 (MQ=255)                                                  tGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAcc                                                                                                     >  3:346199/1‑144 (MQ=255)                                                  tGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCAcc                                                                                                     <  4:346199/144‑1 (MQ=255)                                                     cTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATgg                                                                                                                                      >  2:551637/1‑108 (MQ=255)                                                     cTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATgg                                                                                                                                      <  1:551637/108‑1 (MQ=255)                                                       tAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGtt                                                                                          <  2:106797/150‑1 (MQ=255)                                                         aaGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTa                                                                                        >  2:324001/1‑150 (MQ=255)                                                         aaGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTa                                                                                        <  3:123728/150‑1 (MQ=255)                                                               gCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAgg                                                                                  <  4:131544/150‑1 (MQ=255)                                                               gCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAgg                                                                                  >  2:55614/1‑150 (MQ=255)                                                                 aaaaaCGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGt                                                                                >  2:38954/1‑150 (MQ=255)                                                                         aCCGTTAATATTGACCAGACAATTGCAACTTAATGCTGCATTCAGAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAACGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGGTTCg                                                                        >  4:359790/1‑150 (MQ=255)                                                                         aCCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGT‑ACCGTGGCGTAATgtt                                                                                                                                     <  1:103002/89‑3 (MQ=18)                                                                         aCCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGGTTACTTTATGT‑ACCGTGGCGTAATgtt                                                                                                                                     >  2:103002/1‑87 (MQ=18)                                                                            gTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTAc                                                                     >  4:131957/1‑150 (MQ=255)                                                                                  atTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGa                                                               <  1:31795/150‑1 (MQ=255)                                                                                    tGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATa                                                             <  1:324001/150‑1 (MQ=255)                                                                                       ccAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACa                                                          <  1:419423/150‑1 (MQ=255)                                                                                               ttGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATcgcccgc                                                  <  3:44938/150‑1 (MQ=255)                                                                                                 gCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCa                                                <  2:511065/150‑1 (MQ=255)                                                                                                  cAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCaa                                               >  4:43301/1‑150 (MQ=255)                                                                                                  cAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCaa                                               <  1:38954/150‑1 (MQ=255)                                                                                                    aCTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACt                                             <  3:43301/150‑1 (MQ=255)                                                                                                      ttAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCa                                                                                    >  1:501641/1‑109 (MQ=255)                                                                                                      ttAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCa                                                                                    <  2:501641/109‑1 (MQ=255)                                                                                                      ttAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGa                                           <  3:131957/150‑1 (MQ=255)                                                                                                      ttAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCAGCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGa                                           <  2:403190/150‑1 (MQ=255)                                                                                                          tACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACcac                                       <  3:343589/150‑1 (MQ=255)                                                                                                            cTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAg                                     <  2:38226/150‑1 (MQ=255)                                                                                                             ttcattcaTAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGttt                                                                                         >  2:115053/1‑97 (MQ=255)                                                                                                             ttcattcaTAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGttt                                                                                         <  1:115053/97‑1 (MQ=255)                                                                                                             ttcattcaTAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGCCTTCACCTTGCATGCGTTGACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAgg                                    >  1:658460/1‑150 (MQ=255)                                                                                                               cattcatAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGgca                                  <  3:332836/150‑1 (MQ=255)                                                                                                                attcatAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGgcag                                 <  2:482607/150‑1 (MQ=255)                                                                                                                     tAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCGATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGCTAACATCGCCCGCCAACGGACCCCAGGCAGCATcc                            >  4:315107/1‑149 (MQ=255)                                                                                                                           aTTACGCCACGGTTACTTTATGT‑ACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATatca                      >  2:150216/1‑150 (MQ=38)                                                                                                                           aTTACGCCACGG‑TACATAAAGTAACCGTGGCGTAAt                                                                                                                                        >  4:126086/1‑36 (MQ=255)                                                                                                                           aTTACGCCACGG‑TACATAAAGTAACCGTGGCGTAAt                                                                                                                                        <  3:126086/36‑1 (MQ=255)                                                                                                                             tACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATatcatc                    <  2:674774/150‑1 (MQ=255)                                                                                                                                    cGG‑TACATAAAGTAACCGTGGAGTAATGGCTATCAGACCGCTTTAATGTCGGTGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATcaccacca             >  2:200787/1‑148 (MQ=255)                                                                                                                                                gTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGcac  >  4:158729/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCATAAAACATTACGCCACGG‑TACATAAAGTAACCGTGGCGTAATGGCTATCAGACCGCTTTAATGTCGATGGCTTCACCCTGCATCCGTTTACGCAGGGTACGTTTCGTACGGTCGATAACATCGCCCGCCAACTGACCACAGGCAGCATCGATATCATCACCACGAGTTTTACGCAC  >  NC_000913_3_KO_pgi/2642966‑2643257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.