New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 227052	27 (0.690)	7 (0.190)	7/268	3.8	21.3%	noncoding (1294/2904 nt)	rrlH	23S ribosomal RNA of rrnH operon
	?	NC_000913_3_KO_pgi	2727910 =	NA (NA)					noncoding (1275/2904 nt)	rrlG	23S ribosomal RNA of rrnG operon
Rejected: Coverage evenness skew score above cutoff.

TGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGC  >  NC_000913_3_KO_pgi/226906‑227128                                                                                                                                                   |                                                                              tGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCg                                                                             >  2:541313/1‑149 (MQ=38)  gCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGTTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGc                                                                            <  1:406128/149‑1 (MQ=38)   cACCGAAGCTGCGGCAGCGACGCTTATGCGTTGCTGGGTAGGGGAACGGTGTGTAAGCCTGTGAAGGTGCACTGTGAGGTCTGCTATAGGGATCATAAGTGGGAAGGCTGACATAAGTACCGACACACCGGGTGACAAGCCcgcccgcc                                                                           >  2:251198/1‑149 (MQ=11)    aCCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCg                                                                          <  1:590337/149‑1 (MQ=38)      cGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGa                                                                        >  1:136591/1‑149 (MQ=39)            tGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCa                                                                  >  1:390839/1‑149 (MQ=39)             gCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCaa                                                                 <  2:136591/149‑1 (MQ=38)                   gCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTAAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTc                                                           <  1:2731/149‑1 (MQ=38)                     gACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGAGAAAAGCCCGCTCGCCGGAAGGCCAAGGGTTCCt                                                         >  1:228442/1‑149 (MQ=255)                            aTGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAAc                                                 <  1:369238/149‑1 (MQ=37)                            aTGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAAc                                                 >  1:18692/1‑149 (MQ=38)                             tGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAAGGGTAAGGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACg                                                >  1:334897/1‑149 (MQ=255)                                 ttgttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTaa                                            >  1:115781/1‑149 (MQ=35)                                      gggTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTGTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACAAAGAGTTAGT‑CTCAAACGTTAATCggg                                       >  2:453991/1‑149 (MQ=11)                                        gTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGc                                     >  2:271597/1‑149 (MQ=35)                                                gggTTCTGTAAGCCTGGGAAGTTGTACGGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAg                             <  2:267312/147‑1 (MQ=255)                                                    tCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGa                         <  1:372305/149‑1 (MQ=35)                                                     cTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGAc                        <  1:297071/149‑1 (MQ=34)                                                            ccTGTGAAGGTGTACTGGGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAg                 <  1:49177/149‑1 (MQ=21)                                                              tgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTCACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGACCA‑GAAGCGTTGCTAGTCCAACGATAATCGGGGCAGGGTGAGTCGACCCCTAAGGc               >  1:195231/1‑149 (MQ=11)                                                              tgtgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGCGTCGACCCCTAAGGc               >  2:550473/1‑149 (MQ=25)                                                                tgAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCAT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGa             >  1:16748/1‑149 (MQ=25)                                                                       gtACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGCCGAGGCCGaa      >  1:430439/1‑149 (MQ=21)                                                                        tACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGaaa     <  1:271597/149‑1 (MQ=33)                                                                        tACTGGGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGaaa     <  2:15602/149‑1 (MQ=18)                                                                           tgtgAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGc  >  1:551533/1‑149 (MQ=32)                                                                           tgtgAGGTATGCCGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGc  <  1:460640/149‑1 (MQ=17)                                                                                                                                                   |                                                                              TGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCT‑GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGC  >  NC_000913_3_KO_pgi/226906‑227128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.