| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | 1097372 = | NA (NA) | 6 (0.150) | 6/276 | 4.6 | 19.7% | intergenic (+543/+193) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| ? | NC_000913_3_KO_pgi | 1097531 = | 25 (0.610) | intergenic (+702/+34) | ycdU/serX | putative inner membrane protein/tRNA‑Ser | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGG > NC_000913_3_KO_pgi/1097439‑1097676 | tGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGGGTGCAATATACGAA‑AAAAAAGCCCGTACGTTCGTACGAGCTCTTCTTTAGATATGGCGTGGA‑GGAGGGGATTcacc > 1:128907/1‑146 (MQ=11) gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAGAAGCCCTTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAccc > 2:551193/1‑149 (MQ=25) ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAAcccc > 1:206311/1‑149 (MQ=25) tCACGGTCTGTCCCACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAAcccc < 1:428679/149‑1 (MQ=255) cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAACATGGCGGTGA‑GGGGGGGATTCGACCCCCCGAt > 1:363885/1‑149 (MQ=255) gTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATAc > 1:34342/1‑149 (MQ=40) gttCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCTGGGCTTCTCAGCCCCCCCGTGTGTGCAATCTACGAA‑AAAAAAGCCCGTTCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt < 2:1559/147‑1 (MQ=14) ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGcc < 2:413015/149‑1 (MQ=255) ccAACGTGCTGTCGCCAGTTGTCGAACCCCGGTCGGGTCTTCGCATCCCCCCGGTGTGTGCAATAGACGAA‑AAAAAAGCCCGTACGTACGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGcc < 1:340214/149‑1 (MQ=14) aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGta < 1:636440/149‑1 (MQ=255) gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCACCGGAGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCCTTAAAAATTGCGGGGA‑GGGGGGGAATCCAACCCCCCATACGTTTCCggttacc > 2:304660/1‑143 (MQ=11) tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTCA‑CTGAGGGTTTCACACCCCCGAGACGTTGCCGTATacaca > 1:382857/1‑149 (MQ=14) cgTCGCCGGTTGTCGAACCCCGGTGGGGGATAATCATCACACCGGTGTGTGC‑GGAGAGGAACTGAAAAGCCCGTACTTTCGTACGAGCTCTTCGTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca < 2:154019/148‑1 (MQ=14) tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGc < 2:284710/149‑1 (MQ=255) tCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCCATACGTTACCGTATCCCCACTTTccccgcct > 2:545492/1‑143 (MQ=17) cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCt > 1:55128/1‑149 (MQ=255) ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACATTGCCGTATACAAACTTTCCAGGCGTTCTCCTTCAGCCAcc > 2:456186/1‑148 (MQ=255) cTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGaa > 2:710773/1‑95 (MQ=34) cTTCTCATCCCCCCGGTGTGTGCAATAAACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGaa < 1:710773/95‑1 (MQ=21) gtgtgtGCAATATACGAA‑AAAAAAGCCCGTACTTTAGTACGAGCACGTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCaact > 1:28030/1‑147 (MQ=255) ggtgtgCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAtt < 1:545492/148‑1 (MQ=255) tgtgCAAAATATGAA‑AAAAAAGCCCGTACCTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt < 1:456186/149‑1 (MQ=255) tataCGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGAGGGGATTCGAACCCCCGCTACGTTGCCGTATACACACTTTCCAGTCGTGCTCCTTCCTCCCCTCGGCAAGCTCACCGAATTTACGTTCCt > 2:20300/1‑149 (MQ=14) aaagaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt < 1:483782/149‑1 (MQ=255) aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACCACTCACCACATTGTCGTACCTGTCTTGCtag > 2:289597/1‑147 (MQ=255) | TGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAA‑AAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGG > NC_000913_3_KO_pgi/1097439‑1097676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |