| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_pgi | 4,234,667 | Δ1 bp | intergenic (‑1434/‑499) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 4,234,666 | 0 | A | . | 96.8% | 125.6 / ‑3.2 | 31 | intergenic (‑1433/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (13/17); minor base C (1/0); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG > NC_000913_3_pae_pgi/4234520‑4234810 | tCTATATACGCCGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatc > 2:154041/1‑147 (MQ=255) tataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAtcg < 1:154041/149‑1 (MQ=255) taCGTAGTAGAGATGGACCCCAGGGGCGTTTTATGCTTTCTACGGGATGTGGGCATCGCCTCTGTAAAACTGGCAGCGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAtcgtc < 2:116863/149‑1 (MQ=255) gtaGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTGACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGat < 2:106318/147‑1 (MQ=255) gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGa > 1:328674/1‑138 (MQ=255) gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGa < 2:328674/138‑1 (MQ=255) gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGat > 2:289400/1‑149 (MQ=255) tAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatc > 1:237021/1‑134 (MQ=255) tAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatc < 2:237021/136‑3 (MQ=255) gagaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAACCTGGCAGAGATGA‑GGGGCGTAAATCCCCTGGCCACCCCTAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGGTATTTAg > 2:333146/1‑149 (MQ=255) aTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGcc > 1:281608/1‑149 (MQ=255) ccccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATa > 2:380249/1‑149 (MQ=255) aaGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGc > 1:9804/1‑149 (MQ=255) cGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCa < 2:143741/149‑1 (MQ=255) ttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCTAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCCcgcc < 1:102114/149‑1 (MQ=255) tATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACCCACCTA‑ATGAGGATGCCTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTGGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGGTAAcgccg > 1:98106/1‑149 (MQ=255) ttttACGAGATGTGGGCATCGCTCCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:12798/149‑1 (MQ=255) aCGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAAACCCCTCGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa < 1:95418/149‑1 (MQ=255) cGAGATGTGGGCATCGTCTTTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCAGCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc < 1:352172/149‑1 (MQ=255) cGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGACCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc > 2:68190/1‑149 (MQ=255) gCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa < 1:225529/149‑1 (MQ=255) agggaTTA‑GCGGCGTAAATCCCCTGGCCAACACGAGAATAGATGCATTGAAGAGGCGCTTGCACTG‑ATCATCGTCGAGATTTAGGCCGGATAAGTCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc < 2:98106/146‑1 (MQ=255) gCGTAAATACCCGGGCCACCCCGAGAATAGATGCAGTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGCATAAGGCGTTCACGCCGCATGTGGAAACCGATGCCTTATAAGACGCGCCCGCGTCGCATCAGGcatcggtt < 2:69090/149‑8 (MQ=255) cTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat < 1:380249/149‑1 (MQ=255) tgcccACACCAAGAATAGAGCGATTTAAGAGGCGGTAGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata < 1:131442/146‑1 (MQ=21) tGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCCTCGGCGAAATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCGGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata > 1:388804/1‑149 (MQ=25) tGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata < 2:9804/149‑1 (MQ=255) ggCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGAGAAGCCGTTCACGCCGCATACGGCAACCGTTGCCTGATGCACCGCGGTCGCGTCTTGATAGGCCTACCGGTTCATGCCGatat > 2:122041/1‑149 (MQ=255) gCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGT‑CATCATCGTCGATATATAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCTTGATGCGACGCGGTCGCGTCTTATCAGTCTTACAGGTCTATGCCGATATg > 2:391557/1‑149 (MQ=37) aTAGATGCAGTGAAGAGGCGCTTGCAGTG‑AGCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATTCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc < 1:46503/149‑1 (MQ=255) aTGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa > 1:205904/1‑149 (MQ=255) aTGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa > 2:401088/1‑149 (MQ=255) aTTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACTGATGCCTGATGCGACGCGGTCGCGTGTTGTCAGGCGTACAGGTCGATGCAGATATGTGTATCGGATGCGGCAAGTa > 1:121846/1‑149 (MQ=25) ttGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa < 1:68190/149‑1 (MQ=255) gAAGAGGCGCTTGCAGTG‑ATCATCGTCGAGATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCTACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAGCGTATTCGGCAATTattc < 2:246030/149‑4 (MQ=25) cAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAGATCGTTTTCGGCACTTAATACATAGCACgattg > 1:75889/1‑149 (MQ=255) | TCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTA‑GAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTG > NC_000913_3_pae_pgi/4234520‑4234810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |