BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	2,867,401	Δ1 bp	coding (151/993 nt)	rpoS ←	RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	2,867,401	0	T	.	100.0%	692.1 / NA	142	coding (151/993 nt)	rpoS	RNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (70/72); total (70/72)

Only 100 of 142 total aligned reads displayed.

AAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTC  >  NC_000913_3_pae_pgi/2867256‑2867543

aaGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTc                                                                                                                                           >  3:825363/1‑150 (MQ=255)

 aGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTcc                                                                                                                                          >  1:1395541/1‑150 (MQ=255)

   ttACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCt                                                                                                                                        <  2:192603/150‑1 (MQ=255)

    tACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTg                                                                                                                                       <  2:233742/150‑1 (MQ=255)

      ctctCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGCCGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACCCTGTG‑GGCTCCCTGCg                                                                                                                                     >  2:1115657/1‑150 (MQ=255)

      ctctCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCg                                                                                                                                     <  2:557976/150‑1 (MQ=255)

       tctcGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGa                                                                                                                                    >  2:433500/1‑150 (MQ=255)

            atcatcCGGCGGCGCGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACa                                                                                                                               <  2:900395/150‑1 (MQ=255)

               atcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGtt                                                                                                                            >  3:683830/1‑150 (MQ=255)

                tcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTc                                                                                                                           <  1:1658808/150‑1 (MQ=255)

                tcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTc                                                                                                                           <  1:708422/150‑1 (MQ=255)

                   ggcggcGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACTCGCTGTG‑GGCTCCCTGCGATAACAGTTCctc                                                                                                                        <  2:77345/150‑1 (MQ=255)

                      ggcgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTc                                                                                                                     <  1:139088/150‑1 (MQ=255)

                      ggcgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTc                                                                                                                     >  1:820939/1‑150 (MQ=255)

                        cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCgg                                                                                                                   >  2:1163079/1‑150 (MQ=255)

                               cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATc                                                                                                            <  4:683830/150‑1 (MQ=255)

                                gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCg                                                                                                           >  2:1095695/1‑150 (MQ=255)

                                 aCATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGt                                                                                                          >  4:1033016/1‑150 (MQ=255)

                                  cATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCGAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGtt                                                                                                         <  2:976951/150‑1 (MQ=255)

                                    tctcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTAt                                                                                                       <  1:1637074/150‑1 (MQ=255)

                                     ctcCACGCAGTGCGCGACGCGCAAAATAACCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGTCCAAATAGATATc                                                                                                      >  2:1381861/1‑150 (MQ=255)

                                      tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTAt                                                                                                       <  1:606131/148‑1 (MQ=255)

                                      tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTAt                                                                                                       >  2:606131/1‑148 (MQ=255)

                                      tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCa                                                                                                     >  2:287590/1‑150 (MQ=255)

                                      tcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCa                                                                                                     >  2:945518/1‑150 (MQ=255)

                                       ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCAc                                                                                                    >  2:245794/1‑150 (MQ=255)

                                       ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCAc                                                                                                    >  2:1381429/1‑150 (MQ=255)

                                       ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCAc                                                                                                    >  4:253292/1‑150 (MQ=255)

                                        cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACt                                                                                                   >  3:140629/1‑150 (MQ=255)

                                          cGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTgg                                                                                                 <  1:1381429/150‑1 (MQ=255)

                                           gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTggg                                                                                                <  2:1051313/150‑1 (MQ=255)

                                           gCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTggg                                                                                                >  2:423696/1‑150 (MQ=255)

                                               tGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTcc                                                                                            >  1:542058/1‑150 (MQ=255)

                                                gcgcGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCt                                                                                           <  2:1395688/150‑1 (MQ=255)

                                                    gACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc                                                                                       <  4:561207/150‑1 (MQ=255)

                                                    gACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc                                                                                       >  4:716793/1‑150 (MQ=255)

                                                      cgcgCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTcta                                                                                     <  1:906205/150‑1 (MQ=255)

                                                        cgcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTctact                                                                                   <  3:567960/150‑1 (MQ=255)

                                                         gcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTctacta                                                                                  <  4:961328/150‑1 (MQ=255)

                                                         gcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGATGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTctacta                                                                                  >  1:53044/1‑150 (MQ=255)

                                                           aaaaTCAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTAGCGTCCCACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAg                                                                                <  4:290258/150‑1 (MQ=255)

                                                           aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAg                                                                                <  1:1153500/150‑1 (MQ=255)

                                                             aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGc                                                                              >  3:415592/1‑150 (MQ=255)

                                                                aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGt                                                                                                          >  2:1336103/1‑119 (MQ=255)

                                                                aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGt                                                                                                          <  1:1336103/119‑1 (MQ=255)

                                                                  aCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTccc                                                                                                                                         <  3:155752/86‑1 (MQ=255)

                                                                  aCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCtttt                                                                         >  3:629920/1‑150 (MQ=255)

                                                                   cttcttcttCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTc                                                                        <  1:423696/150‑1 (MQ=255)

                                                                     tcttcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGt                                                                      <  2:289453/150‑1 (MQ=255)

                                                                     tcttcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGt                                                                      <  4:140629/150‑1 (MQ=255)

                                                                      cttcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTc                                                                     <  3:193447/150‑1 (MQ=255)

                                                                       ttcttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCa                                                                    >  2:216801/1‑150 (MQ=255)

                                                                          ttcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCaaaa                                                                 >  1:233727/1‑150 (MQ=255)

                                                                              gCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGTCAAATCGTTATCACTGCGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCt                                                             <  4:825363/150‑1 (MQ=255)

                                                                              gCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCt                                                             >  3:413182/1‑150 (MQ=255)

                                                                               ccGGTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc                                                            <  2:1515989/150‑1 (MQ=255)

                                                                                 gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc                                                            <  3:260331/148‑1 (MQ=255)

                                                                                 gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc                                                            >  4:260331/1‑148 (MQ=255)

                                                                                 gTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCaa                                                          <  4:425500/150‑1 (MQ=255)

                                                                                     aCAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTcc                                                      <  4:629920/150‑1 (MQ=255)

                                                                                         tggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc                                                                                       <  4:201271/113‑1 (MQ=255)

                                                                                         tggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc                                                                                       >  3:201271/1‑113 (MQ=255)

                                                                                           gtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTg                                                                                                  >  4:188369/1‑100 (MQ=255)

                                                                                           gtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                                                >  2:1183041/1‑150 (MQ=255)

                                                                                           gtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                                                <  3:292639/150‑1 (MQ=255)

                                                                                             gAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATc                                                                                                      >  2:423115/1‑94 (MQ=255)

                                                                                             gAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATc                                                                                                      <  1:423115/94‑1 (MQ=255)

                                                                                             gAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACt                                                                                                   >  1:458250/1‑97 (MQ=255)

                                                                                             gAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACt                                                                                                   <  2:458250/97‑1 (MQ=255)

                                                                                              aaTAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatc                                             <  1:994873/150‑1 (MQ=255)

                                                                                                tAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCtt                                                                                                                      <  4:22902/75‑1 (MQ=255)

                                                                                                tAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCtt                                                                                                                      >  3:22902/1‑75 (MQ=255)

                                                                                                   ccAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt                                        >  2:156012/1‑150 (MQ=255)

                                                                                                    cAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc                                       >  2:485079/1‑150 (MQ=255)

                                                                                                     aaTCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc                                                            <  2:637832/128‑1 (MQ=255)

                                                                                                     aaTCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc                                                            >  1:637832/1‑128 (MQ=255)

                                                                                                        ctcACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCCCCCTGCGATAACAGTTCCTCCCCGGCCAAATCGTTATCACTGGGCTCCTGTTCTACTAAGGAAAAAAAAAAAAAAACCTCAACTCCGTTCTCATCAAATTCCGCa                                   <  4:357717/150‑1 (MQ=255)

                                                                                                           aCCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGtt                                                                                                         <  1:243508/77‑1 (MQ=255)

                                                                                                           aCCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGtt                                                                                                         >  2:243508/1‑77 (MQ=255)

                                                                                                                 gTAAAGCTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAttt                          >  2:214028/1‑150 (MQ=255)

                                                                                                                       cTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCa                    >  2:1295932/1‑150 (MQ=255)

                                                                                                                       cTGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCa                    <  3:84104/150‑1 (MQ=255)

                                                                                                                       cTGAGTCGCGTCCAACACACGATGTG‑GGCTCCCTGCGATAACAGTTCCTCTCAGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCCTCAAATTCCGCATCTACATTTAAATCa                    >  4:95826/1‑150 (MQ=255)

                                                                                                                        tGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCAt                   <  1:433500/150‑1 (MQ=255)

                                                                                                                        tGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCAt                   >  2:808176/1‑150 (MQ=255)

                                                                                                                        tGAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCAt                   <  1:1295932/150‑1 (MQ=255)

                                                                                                                         gAGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATg                  >  2:1683734/1‑150 (MQ=255)

                                                                                                                          aGTCGCGTCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGa                 >  2:1283923/1‑150 (MQ=255)

                                                                                                                                 tCCAACACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATGACTGGGTTCCTGTTCTACTCAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCa          >  1:379719/1‑150 (MQ=255)

                                                                                                                                    aaCACACGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCg       >  1:534810/1‑150 (MQ=255)

                                                                                                                                     acacacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGt      >  4:112069/1‑150 (MQ=255)

                                                                                                                                      cacacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTa     <  3:280935/150‑1 (MQ=255)

                                                                                                                                      cacacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTa     >  3:721814/1‑150 (MQ=255)

                                                                                                                                       acacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCg                                                                                                           >  3:899931/1‑47 (MQ=255)

                                                                                                                                       acacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCg                                                                                                           <  4:899931/47‑1 (MQ=255)

                                                                                                                                       acacGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTAt    >  2:549776/1‑150 (MQ=255)

                                                                                                                                         acGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAATTCGTTATCACTGGGTTCCTGTTctact                                                                                   <  1:1212983/69‑1 (MQ=255)

                                                                                                                                         acGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTc  >  1:1118819/1‑150 (MQ=255)

                                                                                                                                         acGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTc  <  2:542058/150‑1 (MQ=255)

                                                                                                                                         acGCTGTG‑GGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTc  >  3:235337/1‑150 (MQ=255)

AAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTC  >  NC_000913_3_pae_pgi/2867256‑2867543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`