New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	2823511 =	50 (1.760)	5 (0.190)	5/266	3.0	10.3%	coding (259/1062 nt)	recA	DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
	?	NC_000913_3_hsa_tpiA	2823549 =	40 (1.480)					coding (221/1062 nt)	recA	DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Rejected: Frequency below/above cutoff threshold.

CGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGC  >  NC_000913_3_hsa_tpiA/2823410‑2823696                                                                                                                                             |                                                                                                                                                    cGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCgg                                                                                                                                            >  1:72372/1‑149 (MQ=255)        gAGCACAGCAGGTTGTCGATATCGACACCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTc                                                                                                                                     <  2:365701/149‑1 (MQ=255)          gCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCg                                                                                                                                   <  2:6718/149‑1 (MQ=255)          ccacaGCATGTTGTGGATATCGACGGCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCg                                                                                                                                   <  2:11349/148‑1 (MQ=255)             cagcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTc                                                                                                                                >  1:132032/1‑149 (MQ=255)                cagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                             >  2:497890/1‑149 (MQ=255)                 agGTTGTCGATATCGACGCCCAGTTGACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTGTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTa                                                                                                                            <  2:414403/149‑1 (MQ=255)                   gTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGTTCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGa                                                                                                                          >  1:345359/1‑149 (MQ=255)                      gTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGAGTTCAGCATCGATAAACGCAGAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTGCCGGAAGATTCCGGTCCGTAGAttt                                                                                                                       >  1:450855/1‑149 (MQ=255)                       tggatatcgcgcttggggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaacCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCAAACGAGGCCGGTCCGTAGATTTc                                                                                                                      <  2:125934‑M1/70‑1 (MQ=255)                              cgcgctgggggcaggtggtctgccgatgggccgtatcgtcgaaatctacggaccggaatcttccggtaaaacCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa                                                                                                               >  1:125934‑M1/73‑149 (MQ=255)                                  gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGc                                                                                                           >  1:250713/1‑149 (MQ=255)                                         ttACGTGCGTAGATTGGGTCCAGCGCTTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCgg                                                                                                    <  2:400291/149‑1 (MQ=255)                                             gTGCGTAGATTGGGTCCAGCGCGAGTTCAGCATCGATAAACGCACAGGTGTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGATTTTATCGGAAGATTCCAGTCCGTAGATTTCGTCGATACGGCCCATCgactga                                                                                                >  2:396072/1‑144 (MQ=255)                                              ggCGTAGATTGGGTCCAGCGCGTGGTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCCGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGac                                                                                               <  1:101191/148‑1 (MQ=255)                                               gCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacc                                                                                              >  1:272132/1‑149 (MQ=255)                                                 gTAGATTGGGTCCAGCTCGTGTTCAGCATCGATAAGCGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGaccac                                                                                            >  2:437408/1‑149 (MQ=255)                                                    gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTa                                                                                                                            >  1:272907/1‑114 (MQ=255)                                                    gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTa                                                                                                                            <  2:272907/114‑1 (MQ=255)                                                      ttGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTCT‑CCCACACTGGCGCGCCTCGGCGATCACCTTCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcc                                                                                       <  1:345760/149‑1 (MQ=255)                                                          tcccAGCGCGTTTTCAGCATCGATAACCGCACACGTTTTACCCTCAC‑GCGGCGCTCCGGCGATCACCTGCAGCGCCAGCGTGGTTTTCCCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCaa                                                                                   <  1:295441/147‑1 (MQ=255)                                                            ccAGCGCGTGTTCAGCATCGATAAACGCACAGTGTTTACCTTCAC‑GCGGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgc                                                                                 <  2:345359/149‑1 (MQ=255)                                                              aGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc                                                                               <  2:132032/149‑1 (MQ=255)                                                              aGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGACCCATCCGCAGACCACCTGCCCCAAccgc                                                                               >  2:93493/1‑149 (MQ=255)                                                                tatcgtcgaaatctacggaccggaatcttccggtaaaaccacgctgacgctgcaggtgatcgccgcagcgcagcgtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCCAGcgcg                                                                              >  1:125934‑M2/78‑149 (MQ=255)                                                                    tgtTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATc                                                                         <  1:154766/149‑1 (MQ=255)                                                                     gtTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATcc                                                                        >  1:166285/1‑149 (MQ=255)                                                                      ttCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCa                                                                       >  2:461865/1‑149 (MQ=255)                                                                       gaaatctacggaccggcctcgtttggtaaaaccacgctgacgctgcaggtgatcgccgcagcgcagcgtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCa                                                                       <  2:125934‑M2/79‑1 (MQ=255)                                                                            ggctaTAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACAGGAAGATTCCGGTACGTAGATTTCGACGATACGGCCCATTGGCAGACCCCCTGCCCCAAGTGCGATATCCAGTGaaa                                                                 >  2:462064/5‑149 (MQ=255)                                                                              cGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGc                                                               <  1:461865/149‑1 (MQ=255)                                                                               gATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCg                                                              >  1:136263/1‑149 (MQ=255)                                                                                    aCGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCg                                                         <  1:497890/149‑1 (MQ=255)                                                                                            gTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt                                                 >  2:233652/1‑149 (MQ=255)                                                                                             ttttACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATg                                                <  2:416744/149‑1 (MQ=255)                                                                                                 aCCTTCAC‑GCTGGGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATATACCGCACCGGGAGACCACCTGCCGCTAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGttt                                            <  2:357272/149‑1 (MQ=255)                                                                                                  tcTTCAC‑GCTGCTCTGCGGCGATCGCCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCGCCAAGAGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTc                                           <  2:290423/148‑1 (MQ=255)                                                                                                   ccgCAC‑GGTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc                                          >  2:250713‑M1/4‑149 (MQ=255)                                                                                                   cTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGGAGGTTGCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc                                          >  2:254392/1‑149 (MQ=255)                                                                                                   cTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGc                                                                                                   <  1:357437/92‑1 (MQ=255)                                                                                                   cTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGc                                                                                                   >  2:357437/1‑92 (MQ=255)                                                                                                   cTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc                                          >  1:338641/1‑149 (MQ=255)                                                                                                    ttCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCca                                         >  1:161667/1‑149 (MQ=255)                                                                                                        C‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATc                                     >  1:437408‑M1/1‑149 (MQ=255)                                                                                                         cgctgacgctgcaggtgatcgccgcagcgcagcgtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATc                                     >  2:112065‑M2/37‑149 (MQ=255)                                                                                                            tgacgctgcaggtgatcgccgcagcgcagcgtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt                                  >  1:138489‑M2/34‑149 (MQ=255)                                                                                                            TGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt                                  >  1:433039‑M1/1‑149 (MQ=255)                                                                                                            TGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt                                  >  1:233652‑M1/1‑149 (MQ=255)                                                                                                                     aggtgatcgccgcagcgcagcgtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTc                         <  2:138489‑M2/125‑1 (MQ=255)                                                                                                                           aCCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCAcc                   >  2:433864/1‑149 (MQ=255)                                                                                                                                   cgtcagcgtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGCCAACCTGCCCCAAGCGCGATATCCAGTGAACGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTGTTCACCCAGGCGCa           >  1:161723/1‑149 (MQ=255)                                                                                                                                      tagcgttGTTTTACCGGAAGATTCGCGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatga        <  2:464730‑M2/142‑1 (MQ=255)                                                                                                                                          gtgGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCCCCCAGGGTCATGATGGa    >  2:20109‑M2/4‑149 (MQ=255)                                                                                                                                            gGTTTTACCGGAAGGTTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGc  <  1:475795‑M2/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    CGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAC‑GCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGC  >  NC_000913_3_hsa_tpiA/2823410‑2823696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.