Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	275,065	0	G	A	100.0%	40.7 / NA	17	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/10);  total (7/10)

CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_hsa_tpiA/275002‑275210                                                                |                                                                                                                                                   cGGGACAAGAAAATCTCTTTTCTGGTCTCACGGCGCTTACTGCTAAATTCACTGTCGGCGAAGATAAGTTAATGACTCATGATGACCCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCg                                                              <  1:957927/149‑1 (MQ=11)   ggACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCggg                                                            <  1:315734/149‑2 (MQ=14)   ggACAAGAAAATCTCCTTTCTGGGCTGACGGCGCGTACTGCTGAATTCACTGTCGGCGAAGATACGTTGCTGACACCAACTGTATCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGa                                                            <  2:431251/149‑1 (MQ=11)    gACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg                                                           <  1:893333/149‑1 (MQ=17)     aCAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAgg                                                          >  1:143498/1‑149 (MQ=18)      cAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGc                                                         >  1:724119/1‑149 (MQ=21)           aaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAt                                                    <  2:463638/149‑1 (MQ=255)               ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGc                                                <  1:357659/149‑1 (MQ=255)                   tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGC‑CCAGCTTATCGAACGATATCTCATATCAGGGACTTGTTTGCACCTTCCGGAGGCGTTATGTGCTggc                                            >  1:1018379/1‑149 (MQ=14)                       tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGcc                                        >  2:1025557/1‑149 (MQ=255)                          tCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGGTGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CATACATGATCTCATATCAGGAACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtt                                     >  2:42917/1‑149 (MQ=255)                             gACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCttttt                                  <  1:958853/149‑1 (MQ=255)                                 gcgcTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAt                              <  2:820371/149‑1 (MQ=255)                                         tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTGTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat                      >  1:124132/1‑149 (MQ=255)                                           ctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttatta                    >  2:496219/1‑149 (MQ=255)                                                         ggagAAGATAAGTTGATGACACATGATGAACCCTGTTCTATGGCTCCAGATGA‑AAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCTGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTa      <  2:85368/146‑1 (MQ=21)                                                             aaGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTaa  <  2:205092/149‑1 (MQ=255)                                                                |                                                                                                                                                   CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGA‑CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAA  >  NC_000913_3_hsa_tpiA/275002‑275210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑