| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 2,033,739 | (T)8→7 | noncoding (91/91 nt) | rseX → | sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 2,033,732 | 0 | T | . | 97.3% | 163.1 / ‑3.2 | 37 | noncoding (84/91 nt) | rseX | sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (18/18); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATCTTTGATATCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTCATGAATATTTATATTTAGAATTCATA > NC_000913_3_hsa_tpiA/2033586‑2033876 | atCTTTGATATCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGAATGATGCCGGCttt > 1:370120/1‑149 (MQ=255) cTTTGATATCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCttttt < 1:225388/149‑1 (MQ=255) atCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGcc > 1:126970/1‑149 (MQ=255) atCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGcc < 1:555506/149‑1 (MQ=255) cgccTTTATTAATTTATTTAATAAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTTTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCtt < 2:370120/146‑1 (MQ=255) taATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAAtgt < 1:322707/149‑1 (MQ=255) tatatTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCa < 1:260480/149‑1 (MQ=255) aCTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTgg > 1:481393/1‑149 (MQ=255) cTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGgc < 1:463642/149‑1 (MQ=255) aTTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTTTTTTTGTTTGCTGCCGGCTTTTTCTT‑TGCCTTCTTTAATGTGCGCCTGATGACCCCACCCGTTTGgcgcc > 2:292774/1‑148 (MQ=255) ataCCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTCTGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:269788/149‑1 (MQ=255) cTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGAt > 2:267015/1‑116 (MQ=255) cTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGAt < 1:267015/116‑1 (MQ=255) tttttATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATTTTTTTTTGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTTATCCCACCAGCCGTTTGTCGCAACAATCTTTGTTAc > 2:100172/1‑149 (MQ=255) attattTTGTTGTATGATGTTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCGTTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCt < 2:229700/149‑1 (MQ=255) attattCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCt > 1:356233/1‑149 (MQ=255) attCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATg > 2:157417/1‑149 (MQ=255) ttCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAAt > 2:136745/1‑95 (MQ=255) ttCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAAt < 1:136745/95‑1 (MQ=255) atgatgCTTCCGTTATTAGCCTTTTATCGGCTTGTTTATATTGTTTGGGCCGGCATGGTGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATGATTGATACCCCCGATCTTTCCGCTcc > 1:126572/1‑148 (MQ=255) gatgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAAc > 1:28850/1‑149 (MQ=255) cTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCAt < 2:356233/149‑1 (MQ=255) tCCGTTATTAGCTTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATaa < 2:126572/149‑1 (MQ=255) tCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATaa > 2:390984/1‑149 (MQ=255) ttattaGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACata > 1:434176/1‑149 (MQ=255) tattaGCTTTTTACCGTCTGGTTTACATTTGTTAGGCCGGCATGATTCCGGC‑TTTTTTTATCCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTCGCGCAACAATCCTTGATACCCCCTAAGTTTCCGCTCAACTCATAACatat < 2:116382/149‑1 (MQ=255) tattaGCCTTTTATCGTTTGGTTTATATTTTTGGGGCGGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACatat < 1:203360/149‑1 (MQ=255) attaGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCa < 2:226923/90‑1 (MQ=255) attaGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCa > 1:226923/1‑90 (MQ=255) ttATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACAt < 1:27606/149‑1 (MQ=255) gTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGc > 2:187917/1‑118 (MQ=255) gTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGc < 1:187917/118‑1 (MQ=255) tCTTGTTTATATTTTTTGTGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACCCCAGCCGTTTGGCGCAACAATCATTGATATTCCCTATGTTTCCGCTCAACTAATAACATATTGATATACATTTATaa > 2:484795/1‑149 (MQ=255) tttATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTc < 2:550358/149‑1 (MQ=255) atataTTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTAAACTCATAACATATTGCTATACATTTATAATTTTCa > 1:567262/2‑149 (MQ=255) tatTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTCATGa > 1:356929/1‑149 (MQ=255) ggCCGGCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTCATGAatatttata > 1:320033/1‑149 (MQ=255) ggCATGATGCCGGC‑TTTTTTTATGCCTTCATTAATGTGCTCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTCATGAAtatttatattta < 2:126970/149‑1 (MQ=255) ccGGC‑TTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATTCATTTATAATTTTCATGAATATTTATATTTAGAATTCATa > 2:123391/1‑149 (MQ=255) | ATCTTTGATATCGGCTTTATTAATTCATTTAATCAATATATTAGCACTGATTACAATTATACCTTTTTATTATTCTGTGTCATGATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGATACCCCCTATGTTTCCGCTCAACTCATAACATATTGATATACATTTATAATTTTCATGAATATTTATATTTAGAATTCATA > NC_000913_3_hsa_tpiA/2033586‑2033876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |