| Read alignment evidence... | |||||||||||
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| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 1,868,865 | 1 | . | G | 20.0% | 122.3 / 39.0 | 45 | intergenic (+23/‑90) | yeaG/yeaH | protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein |
| Reads supporting (aligned to +/- strand): ref base . (14/22); new base G (5/4); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATC > NC_000913_3_hsa_tpiA/1868717‑1869009 | aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt‑t > 1:85034/1‑150 (MQ=255)aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt‑t < 2:85034/150‑1 (MQ=255) aaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TAc < 1:105229/150‑1 (MQ=255) cgccaCCGAAGAGCATAAGAAACACTACGACTTCGCCCACAGTATGAAGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAGGT‑TACCgg < 2:122429/146‑1 (MQ=255) aGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGa < 2:560471/150‑1 (MQ=255) gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTggg < 1:668070/150‑1 (MQ=255) acgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGc > 1:35934/1‑150 (MQ=255) cTTTGTGGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTga < 2:215010/150‑1 (MQ=255) cTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTga < 1:624435/150‑1 (MQ=255) tttGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACCTGT‑TACCCGGCCTACAACGACATCCAACCGTGAGCCTgag > 2:307476/1‑150 (MQ=255) tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAaca > 1:660154/1‑150 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 1:95617/150‑1 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 1:6580/150‑1 (MQ=255) aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa < 2:549115/150‑1 (MQ=255) aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGcca < 1:37586/101‑2 (MQ=39) aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGcca > 2:37586/1‑100 (MQ=39) ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATa < 1:395343/150‑1 (MQ=255) cacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACACGT‑TACCGGGCCTACAACGCCAGCGAGCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt < 1:494896/150‑1 (MQ=255) acCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg > 1:481158/1‑150 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc > 2:573852/1‑150 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACACCGAACCGTGGGCCTGACAAGCGGCCACACAGGCGGAGCATACAGTTGGc > 2:381213/1‑150 (MQ=255) cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa > 2:481082/1‑150 (MQ=255) cGTAAACAGGTGCGTTTACTGTGAGAATGGTATTTGCGCGTACGTAAATCGTCTTACCAACCCTGGCCCGGTCCACATGT‑TACCGGGCCTACAACGACAGCGACCCGTGGACCTGAGAAACGTGAACAACGCGGTAGCCTGCAGTTGGCa > 1:58985/1‑150 (MQ=255) gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg < 2:660154/150‑1 (MQ=255) cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg < 2:64823/150‑1 (MQ=255) gTTTAATGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg < 1:380573/150‑1 (MQ=255) tagttGGGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt < 2:4542/147‑1 (MQ=255) tttGCGCGGCCGTTAAACCTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa < 1:262186/150‑1 (MQ=255) tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAtt < 1:481082/150‑1 (MQ=255) tCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGaa > 2:509526/1‑150 (MQ=255) aacaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTc < 1:424673/139‑1 (MQ=255) aacaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTc > 2:424673/1‑139 (MQ=255) acaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 1:288569/75‑1 (MQ=255) acaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg > 2:288569/1‑75 (MQ=255) ccTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa > 1:175921/1‑150 (MQ=255) gCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCACCACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGCCTGAACGCAAAAAACAGAAgat > 2:213222/1‑148 (MQ=255) gCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAcc < 2:480933/110‑1 (MQ=255) gCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAcc > 1:480933/1‑110 (MQ=255) ccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg < 2:2692/99‑1 (MQ=255) ccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg > 1:2692/1‑99 (MQ=255) ccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa > 2:323559/1‑145 (MQ=255) ccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa < 1:323559/145‑1 (MQ=255) ggTACACATGT‑TACGGGGCCTACAACGCCAGCGCAGCGTTGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGt < 2:381417/150‑1 (MQ=255) gTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTg > 1:663743/1‑150 (MQ=255) ccaTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATc < 1:452850/149‑1 (MQ=255) aTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc > 2:266066/1‑123 (MQ=255) aTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc < 1:266066/123‑1 (MQ=255) | AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATC > NC_000913_3_hsa_tpiA/1868717‑1869009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |