Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,544,021	1	.	A	74.1%	153.3 / 46.9	58	coding (40/1389 nt)	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (7/8);  new base A (20/23);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑G‑C‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCT  >  NC_000913_3_hsa_tpiA/1543875‑1544162                                                                                                                                                       |                                                                                                                                                  tttATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACCCAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGGGATTTCTCCGAGCAATATGTGTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTc‑gc‑gaa                                                                                                                                                  <  1:304953/149‑3 (MQ=255) tttACTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTc‑gc‑gaa                                                                                                                                                  <  1:304959/149‑3 (MQ=255)          aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGCAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑G‑A‑aa‑a‑aagctaa                                                                                                                                     >  1:533026/1‑143 (MQ=255)          aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATa‑a                                                                                                                                     >  1:957586/1‑149 (MQ=255)          aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGGAGACAACTGAGTGATATCCAGAGATTTCTTCGAGCCATATGTTTTCCTTTATTTTCCCGGAAGCCCGGTTTTTCTGGTTTCCA‑GTC‑GC‑G‑G‑AA‑ACAGATa‑a                                                                                                                                     >  1:162612/1‑149 (MQ=255)           ttAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGTTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑Ac                                                                                                                                    >  2:137409/1‑149 (MQ=255)           ttAACGGTAACTGCGCTAAATAGCATCCAGACAAAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑Ac                                                                                                                                    <  2:4151/149‑1 (MQ=255)             aaCGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGa                                                                                                                                  >  1:566319/1‑149 (MQ=255)                 gTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTAt                                                                                                                              >  1:819591/1‑149 (MQ=255)                      tGCGCTAAATAGCATCCAGACACCAAAGGCAAGAAGTAGACAACTGACTAATACCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCTGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTtc                                                                                                                         <  1:355556/149‑1 (MQ=255)                      tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTtc                                                                                                                         <  2:819591/149‑1 (MQ=255)                        cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTtctc                                                                                                                       <  1:148119/149‑1 (MQ=255)                           tAAATAGCATCCAGGAACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCAtt                                                                                                                    <  2:284227/149‑1 (MQ=255)                                aGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                               <  1:790802/149‑1 (MQ=255)                                            caGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                   >  1:860262/1‑149 (MQ=255)                                               aaGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGAATTTTCTGGTTTCCA‑TTC‑GC‑G‑A‑AA‑ATAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTcc                                                                                                >  2:915746/1‑149 (MQ=255)                                                  gCAAGAAGTAGACAACTTACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                             <  1:555911/149‑1 (MQ=255)                                                    aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                           <  1:425432/149‑1 (MQ=255)                                                         gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGTTTTTCTGGTTTCCA‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ACGACTATTTTTCTCATTTTTCAGTACCATATTGTTCCTCACATTcac                                                                                      >  1:480234/1‑149 (MQ=255)                                                          tAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                     >  2:29557/1‑149 (MQ=255)                                                               aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                                >  2:829129/1‑149 (MQ=255)                                                               aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCAGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                                <  1:956975/149‑1 (MQ=255)                                                                       gaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGTTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaa                                                                        <  1:137409/149‑1 (MQ=255)                                                                           tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                            <  2:3855/141‑1 (MQ=255)                                                                           tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                            >  1:3855/1‑141 (MQ=255)                                                                           tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                    >  2:1021680/1‑149 (MQ=255)                                                                              agagTTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                 <  2:957586/149‑1 (MQ=255)                                                                                agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGATTAAATGCA‑GG‑A‑A‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACa                                                               <  1:763086/149‑1 (MQ=255)                                                                                 aaTTACTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCAGGGTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTGTCTCATGTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                              >  2:789338/2‑149 (MQ=255)                                                                                  aTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCCCATGGGCACAAGGGTAGTTGAAAGAAAACaaa                                                             >  2:430279/1‑149 (MQ=255)                                                                                        tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                       <  1:953678/149‑1 (MQ=255)                                                                                        tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                       <  1:29557/149‑1 (MQ=255)                                                                                                    ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                           >  1:17149/1‑149 (MQ=255)                                                                                                    ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                           >  2:144580/1‑149 (MQ=255)                                                                                                     tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAAt                                          >  1:633307/1‑149 (MQ=255)                                                                                                     tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAATCGCAAt                                          >  2:343892/1‑149 (MQ=255)                                                                                                        ccTTTCTTCTCCCAGACGGCTGGATTTTCTGGTTTCCA‑CTC‑GC‑C‑A‑AATA‑AGATA‑ACGAATCTTTTTCTCATGTTGCAGTGCCATTTTGTTCCCCACTTGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                       <  1:430279/149‑1 (MQ=255)                                                                                                        ccTTTATTGTCCCAGGAGGCCGGATTTTCTGGTTTCCG‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                       <  2:186953/149‑1 (MQ=255)                                                                                                         ctttctttTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                      <  2:930573/149‑1 (MQ=255)                                                                                                          tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                 >  1:468879/1‑89 (MQ=255)                                                                                                          tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                 <  2:468879/89‑1 (MQ=255)                                                                                                          tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                         <  1:905852/97‑1 (MQ=255)                                                                                                          tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                         >  2:905852/1‑97 (MQ=255)                                                                                                           cgagtatCCCAGAGGGCCGGATCTACTAGTTTACA‑GTC‑TCGG‑A‑AA‑A‑AGATA‑AGGACCATTTTTCACATTTTGCAGTGCCATATTGTTCCTCACATGCTAACATTGGAAATGAAAAAAAGACAAGACACGAGGTAAGGCGCAATAGCCAg                                    <  2:994459/143‑1 (MQ=255)                                                                                                                   ccAGAAGGGCGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                            <  1:437501/149‑1 (MQ=255)                                                                                                                        aGGCAGGAGTTTGTGGTTGCCA‑GTCTGG‑A‑A‑AA‑A‑AGATA‑ACGAATATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCAGATGCACACATTGGTAATGAAAAAAAGACAAAAGACGAGCTACGGTGCAATAGCCAGTTATTAGAATTaa                       <  2:178432/149‑1 (MQ=255)                                                                                                                         ggCCGGATTTTCTGGTTTCCA‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ATCACTATTTTTCTCATTTTGCAGTGCCTTATTGCGCCGCACATCCCGGATTTTGTAATTAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                      <  1:1021680/149‑1 (MQ=255)                                                                                                                           ccGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAACAAGGAGAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa                    >  1:808662/1‑149 (MQ=255)                                                                                                                           ccGGATTTTCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa                    >  1:785584/1‑149 (MQ=255)                                                                                                                           acGGAGTTTCTGGTTTCCA‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACCTTGGTAATGAAAAAAAGACAAAACACGACGTAAGGCACAATAGCCAATTATTAGAATTAAggc                    >  2:958120/2‑148 (MQ=255)                                                                                                                             ggATTTTCTGGTTTCCA‑GTC‑GC‑G‑A‑AA‑A‑AAAAATCCCCCAATCGTTCGGATGTTGCAGGACCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGACAGTTATTAGAATTAGGGATg                  <  2:480234/149‑1 (MQ=255)                                                                                                                                 tttCTGGTTTCCA‑GTC‑GA‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAAtt              <  1:781823/149‑1 (MQ=255)                                                                                                                                   tCTGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg            <  1:829129/149‑1 (MQ=255)                                                                                                                                    cTGGGTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg           <  2:860262/149‑1 (MQ=255)                                                                                                                                     tGGTTTCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGt          >  2:579804/1‑149 (MQ=255)                                                                                                                                         ttCCA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTGACATGAAGGATGAATTGGGTGaat      >  2:396046/1‑148 (MQ=255)                                                                                                                                           ccA‑GTC‑GC‑GAA‑AA‑A‑AGATA‑ACGACCATTTTTCTCATTTTGCAGTGCCATATTGTCCCCCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTg    <  1:166493/149‑1 (MQ=255)                                                                                                                                            cA‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCCTCCTGCTCACCACATGCCCACATTGGTAATGCAACAAACACAAAACACCAAGTAAGGCGCAATAGCCAGATATTAGAATTAAGGGTGAATTGGGTGAAGtca   >  2:870004/1‑147 (MQ=255)                                                                                                                                             a‑GTC‑GC‑G‑AAAA‑A‑AGATA‑CCGATTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCt  <  1:652091/149‑1 (MQ=255)                                                                                                                                             a‑GTC‑GC‑G‑AAAA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTTGGTGAAGTGCt  <  1:653284/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                  TTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCA‑GTC‑GC‑G‑C‑AA‑A‑AGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCT  >  NC_000913_3_hsa_tpiA/1543875‑1544162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑