Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,679,525	0	G	T	100.0%	59.5 / NA	22	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/6);  total (16/6)

GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_hsa_tpiA/2679382‑2679669                                                                                                                                                 |                                                                                                                                                 gttttttAGTATCAGCGTCTTATTGTAATGGTTTGGTGATGGGCGTGGGGTTTTGCCGATAAAATATTATAGTTGATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                             <  2:30812/147‑1 (MQ=255) ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGa                                                                                                                                             >  1:417841/1‑149 (MQ=255)  gtatttACTATCAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAACAATGCCGCTTTCGGCCAATCCGGAt                                                                                                                                            >  2:367408/4‑149 (MQ=255)  gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                                                                                                            >  1:90878/1‑149 (MQ=255)                           aaTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTAGGGTTAGTAGGGTTTAGTGGACTCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGACCAATCAGGATTAATCGCCGTCAATTCAgagcgagc                                                                                                                   >  2:195964/1‑149 (MQ=255)                                    gcggcgcgCGGTAGCGCGTGACGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata                                                                                                          <  1:195964/142‑1 (MQ=255)                                       atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCACTTCAGCGCGAGCAGACTCATATTg                                                                                                       >  2:369998/1‑149 (MQ=255)                                        tgaGAGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCCTTCGGCCAATCCGGATTAATCGCCGTCAATTCCGCGCGAGCAGACCCATATTgc                                                                                                      >  2:319903/1‑149 (MQ=255)                                                        ccGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc                                                                                      >  1:313133/1‑149 (MQ=255)                                                         cGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAATcc                                                                                     >  2:353158/1‑149 (MQ=255)                                                            tCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCGCGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCcgc                                                                                  >  2:418747/1‑149 (MQ=255)                                                                           aaTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCAGATTTAATCGCCTTCAATTCAGCGCGATCCGACTCATATTGCGCAACCTCCCCGAGTCCCGCCGACGCCAGCATCGc                                                                   >  2:212354/1‑149 (MQ=255)                                                                                  tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCTGCGGACGCCAGCATCGcccccaac                                                            >  2:254160/1‑147 (MQ=255)                                                                                      gTGAACGCGGTTAAAAATAAAAGAAAAGA‑GTTGTGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGc                                                        >  2:302699/1‑149 (MQ=255)                                                                                               atgaaaaaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTCCtc                                               <  2:323187/146‑2 (MQ=255)                                                                                                ttAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGACCAATCCGGATTAATCGCCGTCACTTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCCTCAGGCATTTTTCttt                                              >  2:139008/1‑149 (MQ=255)                                                                                                    aaaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGACCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgc                                          >  2:28559/1‑149 (MQ=255)                                                                                                     aaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgct                                         >  2:181269/1‑149 (MQ=255)                                                                                                        aaaaGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATGGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATgctgct                                      <  1:418747/149‑1 (MQ=255)                                                                                                                                  gCCGCTTTCGGCCAGTCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaa             <  1:346360/149‑1 (MQ=255)                                                                                                                                     gCTGTCGGGCAATCCGGATTAGTCGCCGGCAATTGGGCGCGTGTACACTTTTTTTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa          <  2:299192/149‑1 (MQ=255)                                                                                                                                             ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGCCAACAAGTCAGGtt  >  1:411498/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT  >  NC_000913_3_hsa_tpiA/2679382‑2679669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑