Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,111,482	G→T	intergenic (+797/+115)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,111,482	0	G	T	100.0%	196.7 / NA	64	intergenic (+797/+115)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/34);  total (30/34)

CGAAGTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAT  >  NC_000913_3_hsa_tpiA/4111336‑4111621                                                                                                                                                     |                                                                                                                                            cGAAGTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTTAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTgc                                                                                                                                           <  1:322457/149‑1 (MQ=255)      gcGATATAGTCAGTAGGTGGAGCACAGACCACCTCGGTGTCGGCCGTCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CGCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCAt                                                                                                                                      <  2:770463/148‑1 (MQ=255)        gATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCAttt                                                                                                                                    <  2:741204/149‑1 (MQ=255)          tataGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTa                                                                                                                                  >  1:972940/1‑149 (MQ=255)          tataGGCAGTAGGGGGACCACAAACCACCTCGGTGTCGGAACGAAGCGTAGCCGCCCTGCCCCCGGCGGTTAT‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCACCGAAGAACTTCCTGGATTGCGCCATTTTa                                                                                                                                  >  2:224937/1‑149 (MQ=255)           atgggCAGGAGGCGGCGCACAACCCACGTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCCGCTTCCGTCCTTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTaa                                                                                                                                 <  2:61073/146‑1 (MQ=255)           ataGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTaa                                                                                                                                 <  1:760761/149‑1 (MQ=255)            taGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAAt                                                                                                                                >  1:168915/1‑149 (MQ=255)             aagcagcaGGGGGAGCACAAACCACCCCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAAtt                                                                                                                               <  2:527861/147‑1 (MQ=255)             aGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAAtt                                                                                                                               >  1:479201/1‑149 (MQ=255)                  gTAGGGAGAGCACAAACCCCCTCGCTGTCGCGCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCa                                                                                                                          <  1:873080/149‑1 (MQ=255)                    aGGGGGAGCACGAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGGTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACg                                                                                                                        <  2:489928/149‑1 (MQ=255)                        ggAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTta                                                                                                                    >  1:577675/1‑149 (MQ=255)                               aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGa                                                                                                             >  1:92441/1‑149 (MQ=255)                               aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCACA‑‑GCCAGTGAGTGGGCCCGAGGACTCTGCTGCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGa                                                                                                             >  1:516442/1‑149 (MQ=255)                                  ccaccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCAGCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATa                                                                                                          >  1:153237/1‑149 (MQ=255)                                        cgctgtCGGCCGGCACCTTGCCCTCTTTCAGAGTGCCGATGCG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGaa                                                                                                    <  1:899609/146‑1 (MQ=255)                                        cGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGaa                                                                                                    <  1:187009/149‑1 (MQ=255)                                          gtgtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGa                                                                                                  <  2:727897/149‑1 (MQ=255)                                            gtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCTATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATg                                                                                                <  1:941946/149‑1 (MQ=255)                                               ggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGgcc                                                                                             >  1:290047/1‑149 (MQ=255)                                                    gcACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATACGCGAATAAAGAAAGACGGCCGcccc                                                                                        >  2:612947/1‑149 (MQ=255)                                                    gcACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGcccc                                                                                        >  2:628363/1‑149 (MQ=255)                                                       cacgggCCGAGTTTAGAGTGCGCATGAG‑CTCCCTCT‑GATTCTGCTGCGGCCCGTTCATCTTCCATTTTTCCCCATCGAAGTACTTCCTGTAGTGCGCCATTTTTATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCTGCa                                                                                     <  2:37990/145‑1 (MQ=255)                                                        cTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGTTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAg                                                                                    >  2:295423/1‑149 (MQ=255)                                                        cTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAg                                                                                    >  2:190170/1‑149 (MQ=255)                                                        cTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAg                                                                                    >  2:183197/1‑149 (MQ=255)                                                        cTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAg                                                                                    <  1:183197/149‑1 (MQ=255)                                                             ccGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcag                                                                               >  1:898678/1‑149 (MQ=255)                                                              cgcgTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagc                                                                              <  2:972940/149‑1 (MQ=255)                                                               gcgTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagca                                                                             >  2:614840/1‑149 (MQ=255)                                                                            cGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                <  2:290047/149‑1 (MQ=255)                                                                               tGAT‑CTCCCCCA‑GACTCTTCTTCCGCCCGTTCATCTTCCAGTTTCACCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGta                                                             <  1:612947/149‑1 (MQ=255)                                                                                     tCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                        >  2:113346/1‑149 (MQ=255)                                                                                         ccA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTc                                                    >  2:115219/1‑149 (MQ=255)                                                                                             gACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                 <  2:2278/149‑1 (MQ=255)                                                                                             gACTCTGCGTCCGCCCGGTGATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTTTGTGGAACAGTATAGAGATTCATc                                                 <  2:747779/149‑1 (MQ=255)                                                                                                    cTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaa                                          >  2:744498/1‑149 (MQ=255)                                                                                                     ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                         >  2:62053/1‑149 (MQ=255)                                                                                                         gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgct                                     >  1:132846/1‑149 (MQ=255)                                                                                                                cATCCTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCACCGCCGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgcttt                              <  1:734263/149‑1 (MQ=255)                                                                                                                 aTCTTCCAGTTTCCCCCAACGAAGACCTTCCTGGAGTGCGCCATTTTATTTCTCCACGCTTATAAGCGAATAAAGGTAGATGGCCGCCCAGCAGGGCAGCAGGTATGTGAAACAGTATAGGAATTCAACGGCCCAAAGGGTTTGCtttt                             >  2:171633/1‑149 (MQ=255)                                                                                                                 aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCtttt                             <  2:479201/149‑1 (MQ=255)                                                                                                                  tCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                            <  2:101682/149‑1 (MQ=255)                                                                                                                  tCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                            <  1:113346/149‑1 (MQ=255)                                                                                                                  tCTTCCAGTCTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCGGGGCAGCAGGTCTGTCACACAGCATAGAGACTCATCGGCACAAAGGCGTTGCttttt                            >  2:501239/1‑149 (MQ=255)                                                                                                                    ttCCAGTTTCCCACAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                            <  2:335481/147‑1 (MQ=255)                                                                                                                    ttCCAGTTTCCCACAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                            >  1:335481/1‑147 (MQ=255)                                                                                                                     tgcggTTTCCCCCGACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTTATCGGCACAAAGGCTTTGCTTTTTGTc                         <  2:202508/145‑1 (MQ=255)                                                                                                                      ccAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCa                        >  1:541553/1‑149 (MQ=255)                                                                                                                        aGTTTCCCCCAACGAAGAACGTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAtt                      <  1:496120/149‑1 (MQ=255)                                                                                                                          tttCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCt                                                                        <  1:191226/97‑1 (MQ=255)                                                                                                                          tttCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCt                                                                        >  2:191226/1‑97 (MQ=255)                                                                                                                               cccAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                 >  1:610054/1‑115 (MQ=255)                                                                                                                               cccAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                 <  2:610054/115‑1 (MQ=255)                                                                                                                               cccAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa               <  2:132846/149‑1 (MQ=255)                                                                                                                               cccAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGATTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa               <  2:581636/149‑1 (MQ=255)                                                                                                                                ccAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa              >  2:371663/1‑149 (MQ=255)                                                                                                                                 cAACGAAGAACTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAAc             <  1:115219/149‑1 (MQ=255)                                                                                                                                     gaggatcTTGCTGGGGTGCGGCCTTGTAATTCTCCACGCTTATAAGCGAATCAAGGCAGTGGGCCGCCTCGCAGGGCAGCAGGTCTGGTAATTAGTATAGAGATTCATCTGCACCAAGTCTTTGCTTTTTGTCATTTATTCAAACCTTc         <  2:197307/143‑1 (MQ=255)                                                                                                                                     gaagaaCTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTc         <  1:57386/149‑1 (MQ=255)                                                                                                                                       agaaCTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa       <  1:190170/149‑1 (MQ=255)                                                                                                                                           cTTCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa   >  1:761746/1‑149 (MQ=255)                                                                                                                                            ttCCTGGAGTGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAt  >  1:823011/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                            CGAAGTCGATATAGGCAGTAGGGGGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAG‑CTCCCCCA‑GACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAT  >  NC_000913_3_hsa_tpiA/4111336‑4111621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑