Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	482,230	0	G	A	100.0%	212.0 / NA	63	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/30);  total (33/30)

ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_pae_tpiA/482088‑482373                                                                                                                                                |                                                                                                                                                atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                                           >  1:754603/1‑149 (MQ=255)   aaaCTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCt                                                                                                                                         >  1:126022/1‑149 (MQ=255)       tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                     <  2:87908/149‑1 (MQ=255)       tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                                     <  1:821842/149‑1 (MQ=255)              acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAGTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCCATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                              >  2:459093/1‑149 (MQ=255)               cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                             <  1:154091/149‑1 (MQ=255)                       cgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAc                                                                                                                     >  1:420438/1‑149 (MQ=255)                         gTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGc                                                                                                                   >  2:335570/1‑149 (MQ=255)                            gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                                >  1:687284/1‑149 (MQ=255)                            gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                                >  1:26778/1‑149 (MQ=255)                                  gccGTTCACATTGCTGCCGTCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGGCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACa                                                                                                          <  2:21969/147‑1 (MQ=255)                                  gTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACa                                                                                                          >  1:279811/1‑149 (MQ=255)                                       tCACATAGGTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCa                                                                                                     <  1:711036/149‑1 (MQ=255)                                         acaTCGCGGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGGCGTTGATAGAAACACCCAGCGCCTGCACTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   <  1:119089/149‑1 (MQ=255)                                         acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   >  1:269858/1‑149 (MQ=255)                                         acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   <  2:420438/149‑1 (MQ=255)                                         acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   >  1:565630/1‑149 (MQ=255)                                          caTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAggg                                                                                                  <  1:100061/149‑1 (MQ=255)                                           aTAGCTGCCGCCCCATGCAGCGCCCAGGGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                 <  2:66086/149‑1 (MQ=255)                                            tAGGTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACCCCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTg                                                                                                <  2:584853/149‑1 (MQ=255)                                               cTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATCGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCtt                                                                                             >  2:474115/1‑149 (MQ=255)                                               cTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCtt                                                                                             <  2:126022/149‑1 (MQ=255)                                                  ccgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                          <  1:45617/149‑1 (MQ=255)                                                   cgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CATGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgct                                                                                         <  2:735212/149‑1 (MQ=255)                                                         aTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCt                                                                                   >  1:61087/1‑149 (MQ=255)                                                             aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                               <  1:364762/149‑1 (MQ=255)                                                             aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑ACTGGTCGCTATCAATCTTAAACTGCAGGGTATCTTCGAGACCGTTTGGACGTGAGCTGGTCACCATGTCAGGGTGGTTCGCTGCTTCTgcgc                                                                               >  1:651100/1‑147 (MQ=255)                                                             aGCGACCAGAGTGGTGTTGATGTCGTTGAAAGAACGGAGAGGCGCCTGCGCGTTGT‑CCTGGGCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTCGGCCGTACGCTGGTCAACATATCAGGGTGCTTCGAGGCTTATgcag                                                                               <  2:176048/149‑2 (MQ=255)                                                              gcgcCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACCTATCAGGGTGCTTCGCTGCTTCTgcaag                                                                              >  2:789484/1‑149 (MQ=255)                                                                     gagTGGTGTTAATGCCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTgg                                                                       <  2:579445/149‑1 (MQ=255)                                                                           tgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCATGTTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgc                                                                 >  1:157295/1‑149 (MQ=255)                                                                                aTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGCGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATGTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTAGTTACTCGCCtt                                                            >  1:592188/1‑148 (MQ=255)                                                                                 tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCCTCTCGACCGTTCGAACGTGCGCTGTTCAACATCGTAACAATTTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                           <  2:818354/149‑1 (MQ=255)                                                                                     gTTGATAGAAGCACCCAGCGCCGGCGCTGTTT‑CCTGGTCGATATCAATCTGAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATCACCGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                       <  2:217860/149‑1 (MQ=255)                                                                                     gTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                       <  2:441097/149‑1 (MQ=255)                                                                                     gTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCa                                                       <  1:180270/149‑1 (MQ=255)                                                                                      ttGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTGCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAg                                                      <  1:335570/149‑1 (MQ=255)                                                                                      tggtgaGAAACCGCCAGCTCCTGCGCTGTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAg                                                      <  2:283233/144‑1 (MQ=255)                                                                                           aGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttttt                                                 <  1:810895/149‑1 (MQ=255)                                                                                             aaaCACCCAGCGCCTGCGCTTTTTACAAGTTTCATAT‑TATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCTCCTGAGTCAGTTTTTCg                                               >  2:822667/1‑149 (MQ=255)                                                                                                cacCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                            <  2:188185/149‑1 (MQ=255)                                                                                                   ccAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTCCGCGCCTGAGTCAGTTTTTCGTGACCa                                         >  2:109325/1‑149 (MQ=255)                                                                                                   ccAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                         >  1:266315/1‑149 (MQ=255)                                                                                                   ccAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                         >  1:425773/1‑149 (MQ=255)                                                                                                     aGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                                       <  1:162849/149‑1 (MQ=255)                                                                                                               cTTTTT‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                             >  1:112930/1‑149 (MQ=255)                                                                                                                ttttt‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcccgct                            >  2:737549/1‑145 (MQ=255)                                                                                                                ttttt‑CCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                            <  2:157295/149‑1 (MQ=255)                                                                                                                    t‑CCTGGTCGTTATCAATCGTAAATTTCACGGTATCTTCCAGTCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                        <  2:717748/149‑1 (MQ=255)                                                                                                                        tGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGCCGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCGGTTTTTCGTGACCAAGGCCAGCCTGGTCAACCAg                     >  2:155763/1‑149 (MQ=255)                                                                                                                         ggTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc                    <  1:474115/149‑1 (MQ=255)                                                                                                                          gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCCATCAGCt                   >  2:762960/1‑149 (MQ=255)                                                                                                                          gTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                   >  1:703019/1‑149 (MQ=255)                                                                                                                             gATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGTTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGATTCAGTTTTTCGTGATCAAGGCAAGCCTCGTCAATCAGCtcaa                >  1:256392/1‑149 (MQ=255)                                                                                                                                atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCCGGGTCCTTCGCTGCTTCTGCAACCAACTGGTTACGCGCCTGCGTCAGCTTTTCGTGACCAAGGCCAGCCTGGTCACTCAGCtcaacgt             >  2:777006/1‑149 (MQ=255)                                                                                                                                 tCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGCTCAATCAGCtccaagtc            >  2:376299/1‑149 (MQ=255)                                                                                                                                   aaTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGTTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCCAGGCCAGCCTGGCCAATCAGCtcacagtcaa          >  2:475869/1‑149 (MQ=255)                                                                                                                                     tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   <  1:440775/58‑1 (MQ=255)                                                                                                                                     tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                                   >  2:440775/1‑58 (MQ=255)                                                                                                                                     tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag        <  1:589040/149‑1 (MQ=255)                                                                                                                                     tCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaac        >  1:122031/1‑148 (MQ=255)                                                                                                                                       ttAAACTGCAGGGTATCTTCCAGACCGTTTGGACCTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTATCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc      >  2:672975/1‑149 (MQ=255)                                                                                                                                           aCTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGtt  >  1:693020/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTT‑CCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTT  >  NC_000913_3_pae_tpiA/482088‑482373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑