| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,544,021 | 1 | . | A | 77.1% | 84.6 / 21.3 | 35 | coding (40/1389 nt) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base . (3/5); new base A (12/15); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_pae_tpiA | 1,544,022 | 0 | C | A | 97.1% | 100.1 / NA | 35 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (13/20); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑G‑C‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA > NC_000913_3_pae_tpiA/1543879‑1544170 ||| ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTCGACAACTGACTGATGTCCAGAGATTTCTTCGAGCTATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑G‑A‑a‑aa‑at < 2:643198/149‑2 (MQ=255) gaTTAACGGTAACTGCGCTAAATAGAGTCCTAACACACAAGGGAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTATGGTTTCC‑ATTCG‑C‑G‑A‑a‑aa‑atgtta > 2:823000/1‑144 (MQ=255) ggaccaaaGAGCAGCCAGACACAGAAGGTAAGTAGTAGACATTGGACCGTTAGACAAAGATTTTTTCTAGCAATATGTTTCCCTTTATTTTTCAATAAGGCTGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTtct < 2:58776/144‑1 (MQ=255) gcgtTAAATGGCATCCAGACAAAGAAGGCAAGAAGTAGACAATTGACTGATATCTAGAGAGTTCTTCGAGCAATATGTTTTCCTTTATTTTCCTAGAAGGCCGGATTTTCTGGTTTCC‑AGTAG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTtct < 2:91220/149‑1 (MQ=255) tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCAtt < 1:129520/149‑1 (MQ=255) tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCAtt > 2:790792/1‑149 (MQ=255) tAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCATTTATTTTCCCAGAAGGCCGGATTTTATGGTTTCC‑AGTCG‑A‑GAA‑A‑AA‑AGATAACGACTCTTTTTCTCAtttttc > 2:543937/1‑147 (MQ=255) tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCC‑C‑GAC‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGc > 2:469092/1‑149 (MQ=255) acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGACGTGCCATATTg > 2:33487/1‑149 (MQ=255) aGAAGGCAAGAAGTAGACAACTGAATGATATGCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt < 2:562077/149‑1 (MQ=255) aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAAGTAC‑G‑A‑A‑A‑AA‑AGATAACGACTATTTTTATCAGTTTGCAGTGCAATATTGTTCCTcaca > 1:834460/1‑149 (MQ=255) agaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt < 1:790792/149‑1 (MQ=255) agagAACTGACTTATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGCAGCC‑A‑A‑A‑AA‑AGATCACGATTAGTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacac < 1:200728/149‑1 (MQ=255) aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGCCG‑C‑GAA‑A‑AA‑AAATACCGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg < 2:353935/149‑1 (MQ=255) gactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa < 1:255650/149‑1 (MQ=255) acttaTATCCATAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCC‑C‑C‑A‑ACAA‑AGATACCGACTCTTTTCCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGTTAAt < 2:202959/149‑1 (MQ=255) gaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑G‑AAA‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGAGATATTGTTCCTGACATGCACAAATTGGTAATGaa > 1:277962/1‑149 (MQ=255) taggcAGAGATTTGTTAGAGCAATATGTGTTCGTTTATTCTCCCAGAAGGCTGGTTTTTCTTGTTTCC‑AGTAG‑C‑G‑A‑A‑AAGAGCGAACGACTGTTTTTCTCATTTTACTGTCCGATATTGTTCCTCACATGCACAAATTGGTAATGaata < 2:112584/145‑3 (MQ=255) tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGGACACATTGGGAATGaaaaaa > 1:172408/1‑149 (MQ=255) tcAGAGATTTTTTCGAGCAATTTGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa < 1:1294/148‑1 (MQ=255) tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCCCAATTTCACACATTGGTAATGAAAAAAAGACaaaa < 1:204770/149‑1 (MQ=255) cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCAGTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac > 1:624789/1‑149 (MQ=255) tcGAGGAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 2:809418/149‑1 (MQ=255) tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 2:68728/149‑1 (MQ=255) aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGt > 1:271370/1‑149 (MQ=255) atGTGTTACTTTAATTTCCCAGAAGGTCGGATTTTCTGGGGTCC‑AGTCG‑CGG‑A‑A‑AA‑AGATAACGTCTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACTTGCAAACAATGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcg < 2:631838/149‑1 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa < 1:82429/149‑1 (MQ=255) gAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAtt > 2:245554/1‑149 (MQ=255) gCCGGATTTTCTGGTTTCC‑AGTCG‑C‑GAG‑A‑AA‑AGATAACGACTATTTTTCTCCTTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGAGCAATAGCCAGTTATTAAAATTAAgg > 2:827191/1‑149 (MQ=255) ccGGATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa > 1:459280/1‑149 (MQ=255) gATTTTCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGa < 1:235002/149‑1 (MQ=255) tCTGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg > 1:346126/1‑149 (MQ=255) tCTGGATTCC‑AGTAG‑C‑GAA‑A‑AA‑AAATAACGACTATTTTCATTAATTTGCAGTGCTATATTGTGCCTCACATGCACAAAAAAAAAAAAAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg < 2:479388/149‑1 (MQ=255) tGGTTTCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGTAATggat < 2:8917/149‑3 (MQ=255) ttCC‑AGTCG‑C‑GAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAg > 1:312143/1‑149 (MQ=255) tcgcgaaa‑aa‑aGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGa > 1:777777/8‑149 (MQ=255) cgcgaaa‑aa‑aGCTAACGACTATTTGTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACAAATTGGTAATGAAAAACAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAt < 2:700422/143‑1 (MQ=255) aa‑aa‑aGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa < 1:245554/148‑1 (MQ=255) aa‑aa‑aGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa < 1:47757/148‑1 (MQ=255) ||| TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCC‑AGTCG‑C‑G‑C‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA > NC_000913_3_pae_tpiA/1543879‑1544170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |