| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,296,950 | 1 | . | T | 20.0% | 47.8 / 13.0 | 20 | coding (1172/2676 nt) | adhE | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase |
| Reads supporting (aligned to +/- strand): ref base . (6/10); new base T (2/2); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCG > NC_000913_3_pae_tpiA/1296816‑1297089 | tCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGa < 1:211247/149‑1 (MQ=255) ccAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCACAACCATTTAAGTGGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGaa < 1:268627/149‑1 (MQ=255) cGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑Taa < 1:151422/149‑1 (MQ=255) gAGTTACCACCCCAAGTACCACAACCCAGAGTCAGGGCAGGTGCGAGTTTGAAGTTATAGAGGTCACCGATACCACCGTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCgc < 2:467218/149‑2 (MQ=255) ccAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCt < 2:9226/149‑1 (MQ=255) gAACCACAACCCAGAGTCGGGGAAGGTGCGAGTTTGAAGTTATGCATGGCCCAGATCACACGCTCCGAAGCTGGGGTGTTAATCAGGATACGCGCCGTTTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGt > 2:360295/1‑149 (MQ=255) gAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTAACCGATACCACCCTTAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGt > 2:107887/1‑149 (MQ=255) aaCCACAACCCAGGGTCAGGGAAGGGGCGAGTTTGAAGTTATGCAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCa < 2:128736/149‑1 (MQ=255) cacaACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACACTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGGGCCGGTTGGTTATCCTGGTCAgtg > 1:214699/1‑149 (MQ=255) aGGGGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGAc < 2:330459/149‑1 (MQ=255) tGCGAGTTTGAAGTTATACAGGCCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGa < 1:401913/149‑1 (MQ=255) gTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTc < 1:284264/55‑1 (MQ=255) gTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTc > 2:284264/1‑55 (MQ=255) cGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACCGGTAAGAGGGATGACCGATACCGCCCATAGCACCCCGTTTatct > 1:347139/1‑149 (MQ=255) caccCTGAGACGCTGGGGTGTTAATCGGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATCCCGCCCATAGCAACCAGTTTCTCTGCtttt > 2:123273/1‑149 (MQ=255) tGAGACGCTGGCGTGTTAATCAGGATACGCGCCG‑TTTTCATTTT‑TGTGTGGAGTTAAGCAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGCGGTATGACCGATACCGCCCATACCATCCAGTTTCTCTGCGTTTTTTAc < 2:358752/149‑1 (MQ=255) tCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTa > 1:406775/1‑149 (MQ=255) gATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATAACGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcgc > 1:478402/1‑149 (MQ=255) tACGCGCCGTTTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg < 1:107887/149‑1 (MQ=255) tACGCGCCGTTTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTACGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg < 1:360295/149‑1 (MQ=255) | TCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCG‑TTTTCATTTTCTGACCGAAG‑TAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCG > NC_000913_3_pae_tpiA/1296816‑1297089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |