Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,544,022	C→A	100%	L13F (TTG→TTT)	narU ←	nitrate/nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,544,022	0	C	A	100.0%	239.5 / NA	74	L13F (TTG→TTT)	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/39);  total (35/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA  >  NC_000913_3_pae_tpiA/1543879‑1544157                                                                                                                                                  |                                                                                                                                           ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaa                                                                                                                                     >  1:851144/1‑148 (MQ=255) ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGAAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaa                                                                                                                                     <  1:110136/149‑2 (MQ=255)  tGAGATTAACGGTAACTGCGCTAAATATCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaag                                                                                                                                    <  1:179891/149‑3 (MQ=255)  tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaag                                                                                                                                    <  1:180247/149‑3 (MQ=255)   gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATATTTTCATTTATTTTCCTAGAAGACAGGATATTCTTATATTCAGTCG‑CG‑A‑a‑aaaaca                                                                                                                                   >  2:428768/1‑146 (MQ=255)    agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCACAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑a‑aaaagat                                                                                                                                  >  1:923045/1‑145 (MQ=255)       tcaaCGGTAACTGCGCTAAATAGCATCCACACACAGAAGGCAAGAAATAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAAc                                                                                                                                <  2:887653/147‑1 (MQ=255)           cGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACt                                                                                                                            >  2:426672/1‑149 (MQ=255)                 cTGCGCTAAATCGCATCCAGACCCAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTAttttt                                                                                                                      <  1:384252/149‑1 (MQ=255)                  tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtc                                                                                                                     >  2:1180533/1‑149 (MQ=255)                  tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCAAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtc                                                                                                                     >  2:412705/1‑149 (MQ=255)                       tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:1420304/1‑149 (MQ=255)                       tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:645217/1‑149 (MQ=255)                       tAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtt                                                                                                                >  1:645065/1‑149 (MQ=255)                        aaaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTtctcctct                                                                                                               >  2:1109037/1‑145 (MQ=255)                        aaaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAttt                                                                                                               <  1:119670/149‑1 (MQ=255)                         aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCAtttt                                                                                                              <  1:1102009/149‑1 (MQ=255)                           tAGCATCCAGACACAGAAGGCAAGAAGGAGACAAATGACTTATATCCAAAGATTTCTTCGAACAATATGTTTTCCTTTATTTTCCCAGAAAGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGc                                                                                                            <  2:620505/149‑1 (MQ=255)                            aGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCa                                                                                                           <  1:422166/149‑1 (MQ=255)                             gCATCCAGACACAGAATGCAATAAGTAGACAACTGACTGATATCCAGAGATTCCTTCGAGCAATATGTTTCCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAg                                                                                                          <  1:1167453/149‑1 (MQ=255)                                 ccAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGGTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTGTTTTTCTCATTTTGCAGTGcc                                                                                                      >  2:366864/1‑149 (MQ=255)                                  cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCGTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑GGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCa                                                                                                     >  1:35163/1‑149 (MQ=255)                                         aGAAGGCAAGAAGTAGACATCTTACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              <  2:226852/149‑1 (MQ=255)                                         aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              >  2:1304167/1‑149 (MQ=255)                                         aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              <  1:99179/149‑1 (MQ=255)                                         aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                              >  1:1101704/1‑149 (MQ=255)                                          gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                             >  1:862756/1‑149 (MQ=255)                                            aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                           <  2:1383710/149‑1 (MQ=255)                                                aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                       <  2:130468/149‑1 (MQ=255)                                                  gaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATg                                                                                     <  1:721239/149‑1 (MQ=255)                                                   aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGc                                                                                    >  1:349579/1‑149 (MQ=255)                                                        gACAACTGACTGATATCCAGATATTTCTCCGAGCACTAGGTCTTCCATTATTTGCACAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCCTTTTGCAGTGCCATATTATTCCTCAAATGcac‑aca                                                                              >  2:671650/1‑149 (MQ=255)                                                        gACAACTGACTGATATCCAGAGATTTCTTGGAACCAGAGAGTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGACATATTGTTCCTCACATGtac‑cca                                                                              >  2:671694/1‑149 (MQ=255)                                                         aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACAt                                                                             >  1:95161/1‑149 (MQ=255)                                                           aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  2:182061/1‑149 (MQ=255)                                                           aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  2:1159595/1‑149 (MQ=255)                                                           aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTg                                                                           >  1:1021711/1‑149 (MQ=255)                                                                actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAAt                                                                      <  2:839389/149‑1 (MQ=255)                                                                        ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGaaaaaaa                                                              >  2:217161/1‑149 (MQ=255)                                                                        ccAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTGTCCACAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGaaaaaaa                                                              <  1:264734/149‑1 (MQ=255)                                                                        ccAGAGATTTCTTCGAGCAATATGCTTTCGTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTTACATGCAC‑ACATTGGTAATGaaaaaaa                                                              <  2:1359971/149‑1 (MQ=255)                                                                            agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACa                                                          >  1:722291/1‑149 (MQ=255)                                                                                    tcGAGCAATATGTTTTCCCTTCTTTGCCCAGAAGGCCTGATTTTCTGGTTTCCAGCCG‑CGAA‑A‑AAAGATAACGACTATTTCTCTCACTGTGCAGTGACATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGa                                                  <  1:631096/149‑1 (MQ=255)                                                                                      gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAgg                                                <  1:31092/149‑1 (MQ=255)                                                                                       ggCAATAGGTTTTCCTTTATTGTCCCAGAAGGCCGTATTTTCTGCTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGt                                               <  2:851144/148‑1 (MQ=255)                                                                                         gaaTTTGTTTTCCTTTATTTTTCTAGAAGGTCGGATTTTCTGGTTTCCAGTCA‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                             <  2:1209819/148‑1 (MQ=255)                                                                                         cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                             <  1:1166511/149‑1 (MQ=255)                                                                                         cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGCaa                                             >  2:687391/1‑149 (MQ=255)                                                                                            tatGTTTTCCTTTATTTTCCCAGGAGGCCGGATTTTCGGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                          <  2:1272656/149‑1 (MQ=255)                                                                                            gatGTATTCCTTTATTTGCCCAGGAGGCCGGATTTTCTGTTTTCCAGTCG‑TGAA‑A‑AAAGATAACGACTATTTTTGTCATTTTGCAGTGCCATATTGTTCCTCACAGTTAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                          <  2:566266/148‑1 (MQ=255)                                                                                                ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                      >  2:1215696/1‑149 (MQ=255)                                                                                                    ccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                  <  1:795013/149‑1 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                >  1:646193/1‑149 (MQ=255)                                                                                                           tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACACTGGTAATGAAAAAAAGACAAAACACGAGGAAAGGCGCAATAGCCAGttat                           >  2:911891/1‑149 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACGTGTAT‑TGAGGTTTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCAAGTTATTAGaa                      >  1:695114/1‑149 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      <  1:374359/149‑1 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      >  2:931951/1‑149 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCAATTGCATAAAAGTGGT‑ATGAAAAAAAGACAAAACACGAGGTAAGGGGCAATAGCCAGTTATTAGaa                      >  1:694702/1‑149 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCCGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATGTTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                      <  1:310009/149‑1 (MQ=255)                                                                                                                    agtccGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                  <  2:952800/146‑1 (MQ=255)                                                                                                                    aGGCCGCAGTTTGTGGTTCCATGTCG‑CC‑A‑ATAAAGATAACTACTATTTTTTTAATTTTGCAGTACCATATTGTTCCTCCCATTCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                  <  2:318451/149‑1 (MQ=255)                                                                                                                     ggCCGGATTTTCTTGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                 <  1:683867/149‑1 (MQ=255)                                                                                                                     ggCCGGATTTTCTGGTTTCCAGTCGCCA‑A‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                 <  1:176930/149‑1 (MQ=255)                                                                                                                        cGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATCTTTTTCGTCAAATGGGT‑ACATTGGTAATGAAAAAAAGACAAAACAGGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt              >  1:1402052/1‑149 (MQ=255)                                                                                                                        cGGATTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTAAGATATTGTTCCTCAAATGTAC‑AGAGTGGTAATGAAAAAAAGACAAAACACAAGGTAAGGCGCAATAGCCAGTTCTTAGAATTAAGGAt              >  1:1401906/1‑149 (MQ=255)                                                                                                                           aTTTTTGGGTGTTCAGTCG‑CGAA‑A‑AAAAATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa           <  2:91567/149‑1 (MQ=255)                                                                                                                           aTTTTCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa           <  2:236580/149‑1 (MQ=255)                                                                                                                               tCTTGTTTCCAGTCG‑AGAA‑A‑AAACATAACGACTATTTTTCGCATTTTGCGGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg       <  1:964677/149‑1 (MQ=255)                                                                                                                               tCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCACAATAGCCAGTTATTAGAATTAAGGATGAATTgg       <  2:349579/149‑1 (MQ=255)                                                                                                                               tCTGGTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAAAACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTgg       >  1:656778/1‑149 (MQ=255)                                                                                                                                  ggTTTCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg    >  1:130984/1‑149 (MQ=255)                                                                                                                                  ggTTTCCAGTCG‑CG‑AAA‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg    <  1:366864/149‑1 (MQ=255)                                                                                                                                    tttCCAGTCG‑CGAA‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa  <  1:1304167/149‑1 (MQ=255)                                                                                                                                    tttCCAATCG‑CGAA‑A‑AAAGATAACGTCTAGTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑AAAATGGAAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa  <  2:778488/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                           TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑A‑AAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCAC‑ACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAA  >  NC_000913_3_pae_tpiA/1543879‑1544157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑