New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3908495 =	43 (0.980)	6 (0.140)	6/272	5.0	13.7%	intergenic (‑129/+54)	pstB/pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease
	?	NC_000913_3_pae_tpiA	3908518 =	34 (0.800)					intergenic (‑152/+31)	pstB/pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCGCAAACGGG  >  NC_000913_3_pae_tpiA/3908379‑3908663                                                                                                                                             |                                                                                                                                                  cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                          <  2:108220/149‑1 (MQ=255)    aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACTGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                                       >  2:617845/1‑149 (MQ=255)                    ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                                  >  1:166649/1‑138 (MQ=255)                    ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                                  <  2:166649/138‑1 (MQ=255)                           aTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                                                >  2:293658/1‑149 (MQ=255)                               ggCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGAGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCG‑CCACCCACAAGACATTCTTCGGGGCAGGGGAGAACCGTGTCAGCAATATCAACCgtg                                                                                                            >  1:760585/1‑149 (MQ=25)                                       gTAGTCCGGATCAGGGGTTGCGGTCGCTTCCGGCAAGGTGTTAACGGTGTACAGGGTCAAAAGGGCGCTCGGTCGGCCCCCTCG‑CCCCTCTGGGGGGGGGGTTAGGGTGAGGGGAAAACTGTGTCAGCAATATCAACCGTGTTTAttct                                                                                                    <  1:657895/149‑1 (MQ=25)                                                tacgggCGTTCCCGCCTCTCCCGGAAAAGGGTTAACCGTGTACAGAGCCAAAAAGGCGCTGGGTCGGTCCCCCCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaaa                                                                                           <  1:564217/145‑1 (MQ=37)                                                    ggCTTTCTCGCGGCACCCGGCAAAGTGTTACCCGTGCACAGAGTCAAAAAGGCGCGCGCTCGGCCCCCTCG‑CCCCTCGGGGGGGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                                       <  1:559797/149‑1 (MQ=37)                                                             gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAACACAACGCGCGCCac                                                                              >  2:9873/1‑148 (MQ=255)                                                             gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAg                                                                              >  1:255386/1‑149 (MQ=255)                                                              ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGACGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGATCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACACGACCCAGa                                                                             >  2:641696/1‑149 (MQ=38)                                                                 cttCCGGCAAAGTGTGAACTGTGTCGAGAGTAAAAAAGTCGCTCGGTCGGCCCCCTCG‑CCCCTCGGGGGAGGGGGTTGGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATg                                                                          <  1:570549/147‑1 (MQ=255)                                                                   gccGACAAAGTGTAAACCGTGTACAGAGCCAAAACGGCGCCCGGTCGGTCCCCTCC‑CCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                                        <  1:534191/148‑1 (MQ=38)                                                                    ccGGCAAAGTGTTAACGGTGTACAGGGTCAAAAAGGCGCCGGGCCGGTCCCCGCC‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                                       <  2:343952/149‑1 (MQ=38)                                                                                     gtgtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                                      >  1:424167/1‑149 (MQ=255)                                                                                      tttACAGAGTAAAAAAAGGGCATGGGCGGCCCCCACG‑CCCCTAAGGGAAAGGGGTTAGGGTAAGGGGAAAACCGTATCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                                     <  2:575065/147‑1 (MQ=255)                                                                                      tgtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                                     >  1:63541/1‑149 (MQ=255)                                                                                              gTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                             >  1:106585/1‑149 (MQ=255)                                                                                               tCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGGGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                                 <  1:490714/112‑1 (MQ=255)                                                                                               tCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGGGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                                 >  2:490714/1‑112 (MQ=255)                                                                                                 aaaaaGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCaa                                          <  1:293658/149‑1 (MQ=255)                                                                                                 aaaaaGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCAAAAACACCGCGCGCCAGAATGTTAAGCCGCAGTACGCCCAGGGGAAGCATCaa                                          >  2:40523/1‑149 (MQ=37)                                                                                                    aaGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAc                                       >  1:391069/1‑149 (MQ=255)                                                                                                          cTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGGTAGGGTGAGGAGAAAACAGTGACAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCACTACGCACAGGGTAATGATCAATACCCCgac                                 >  2:341640/1‑147 (MQ=39)                                                                                                           tcggtcggTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                                >  1:632993/1‑149 (MQ=255)                                                                                                             ggtcggtcCCCTCG‑CCCCTCTGGGGGGAGGGTTAGGGTGAGGCGAAAACCGTGACAGCAATAGCAACGGTGTTTATTGTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCa                              >  2:631082/1‑149 (MQ=38)                                                                                                               tcggtcCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACCCACAGGGTAATGATCAATACCCCGGCCCAgg                            >  2:131046/1‑149 (MQ=255)                                                                                                               tcggt‑cCCTCGCCCCCCCTGGGCGGAGGGTTAGGGTGGGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAgg                            <  1:127530/149‑1 (MQ=255)                                                                                                                 ctaacccTCT‑CCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGcc                          >  2:754435/5‑149 (MQ=255)                                                                                                                  gtcCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa                         >  1:369482/1‑149 (MQ=255)                                                                                                                  gtcCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa                         >  1:369600/1‑149 (MQ=255)                                                                                                                       cTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                           >  1:600474/1‑142 (MQ=255)                                                                                                                       cTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                           <  2:600474/142‑1 (MQ=255)                                                                                                                             cccagaggggCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGCTCAATACCCCGGCCCAGGCCAATTGCTGccc               >  2:486333/7‑148 (MQ=255)                                                                                                                               ctctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt             >  1:487481/1‑149 (MQ=255)                                                                                                                                          gggTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCGCAAACggg  <  2:424167/149‑1 (MQ=255)                                                                                                                                          gggTTAGGGTGAGGGGAAAACCGGGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCGCAAACggg  <  1:177000/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  CTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCG‑CCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCGCAAACGGG  >  NC_000913_3_pae_tpiA/3908379‑3908663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.