New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	4139396 =	29 (0.740)	4 (0.110)	4/270	5.2	11.7%	coding (229/663 nt)	fsaB	fructose‑6‑phosphate aldolase 2
	?	NC_000913_3_pae_tpiA	4139434 =	33 (0.900)					coding (191/663 nt)	fsaB	fructose‑6‑phosphate aldolase 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCA  >  NC_000913_3_pae_tpiA/4139291‑4139578                                                                                                                                                |                                                                                                                                                  ataCCGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCgg                                                                                                                                              <  1:140231/149‑1 (MQ=255)  taCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGc                                                                                                                                             >  1:486871/1‑149 (MQ=255)     agcagTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTGCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCCCa                                                                                                                                          >  2:188247/1‑149 (MQ=255)         gTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAGCCCCTGCGCGTCGCGGCTCATGGt                                                                                                                                      <  2:278182/149‑1 (MQ=255)          tGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTc                                                                                                                                     >  1:476836/1‑149 (MQ=255)          tGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGACAGTCACCGGGATTGTCCCCACAATACCCGGAATAGCGTCGCGCGGGCGCTGCGCTTCTTCCACCATCCCCAGCGCGTCGCGGCTCATggac                                                                                                                                     >  1:147895/1‑147 (MQ=255)                                ttttttCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCtcat                                                                                                              >  2:668897/1‑149 (MQ=255)                                  gtttCAGTATTTTAATTGCTGCCAGACCTTAGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCtcatca                                                                                                            <  2:525700/148‑1 (MQ=255)                                        gTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GGGCAAACAGAATGCCCTCATCACCAATc                                                                                                      <  2:670742/149‑1 (MQ=255)                                               aaTTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCACCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCttttt                                                                                               <  1:304839/149‑1 (MQ=255)                                                     tgcCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGaa                                                                                         >  1:217954/1‑148 (MQ=255)                                                       ccAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAACCGCTTTTTGAAGAcgc                                                                                       >  2:200443/1‑149 (MQ=255)                                                              ttCGGAAGTCACCGGGATTTTCCCCACAATGCCCGGAATAGCGTCGCGCAGGCGGTTCGCTACTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGggagca                                                                                <  2:519122/149‑1 (MQ=255)                                                                        aCCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCACGTCGCGGCTCCTGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCatat                                                                      >  2:640957/1‑149 (MQ=255)                                                                         ccGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATg                                                                     <  1:140089/149‑1 (MQ=255)                                                                         ccGGGATTGTCACCACAACAGCCGGCATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGATTTTTGCAGACGCGGCAGCACTTCCCATATg                                                                     <  2:529624/149‑1 (MQ=255)                                                                          cGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATgg                                                                    >  1:277676/1‑149 (MQ=255)                                                                           gggATTTTCACCACACTACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGa                                                                   <  1:128432/149‑1 (MQ=255)                                                                                      cacaATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTgg                                                        <  2:573051/149‑1 (MQ=255)                                                                                      cacaATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTgg                                                        >  2:355312/1‑149 (MQ=255)                                                                                             cccGCAATAGGGTCGCGCAGGCGCTTCGCTGCTTCCACCATCGCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGTTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGAt                                                 <  2:102245/149‑1 (MQ=255)                                                                                                     aGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCg                                         <  2:44625/149‑1 (MQ=255)                                                                                                             gcAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTAGCAGCGATAATGCTCGggttggtt                                 >  1:181801/1‑149 (MQ=255)                                                                                                                    cttcgcttcTTCCACCATCCCCTGCGCGTCGCGGCTCCTGGTATGGGGGCGACCCCGTCGGCT‑GTCTGTTATCGCTTTTTGCCGACGCGGCAGCACGTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCAccc                          <  2:486871/149‑1 (MQ=255)                                                                                                                            cctcCACCACCCACTGCGCGTCGCGGCTCATGGTAT‑GAGCAAACAGAATGCTCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGTACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATg                  <  2:227124/147‑1 (MQ=255)                                                                                                                              tcCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTTCAGACTCGGCAGCCCTTCCCCTATGCACTCCTTGCTGCCACCTATAATGCTCGGTCTACTGGTCACCCCGGctacgcg                >  2:569003/1‑143 (MQ=255)                                                                                                                              tcCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATggg                <  1:588198/149‑1 (MQ=255)                                                                                                                                caccaTCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCCTCCACATTCGCGTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGa              <  1:668897/149‑1 (MQ=255)                                                                                                                                caccaTCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCGCGGGTGAGTTGTCACGCCGGCACTggggc              >  1:656055/1‑148 (MQ=255)                                                                                                                                 accaccCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGaa             >  2:597012/1‑149 (MQ=255)                                                                                                                                 accaTCCCCTGCGCGTCGCGGCTCATGGTCC‑CTGCAATCATCATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGaa             <  1:359631/149‑1 (MQ=255)                                                                                                                                    aTCCCCGGCGCGTCGCGGCTCATAGGAT‑GAAAAAACAGAATGCCCGCATCACCAACCGCTTTGTGCAGACGCGGCAGCAATTCCCATATGGACTCCTTTACGGCAGCGATAATGCTCAGGTTAGTTGTCACCCCGGCAAGGGGGActat          >  2:636377/1‑149 (MQ=255)                                                                                                                                     tCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCCATGGGGAATATg         >  2:660511/1‑149 (MQ=255)                                                                                                                                         cTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTg     >  2:356490/1‑149 (MQ=255)                                                                                                                                         cTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGCGGAATACGccgg     >  2:512821/1‑146 (MQ=255)                                                                                                                                          tGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGc    <  1:356490/149‑1 (MQ=255)                                                                                                                                            cgcgTCGCGGCTCATGGTCG‑GAGCAATCAGTATGCTCTCAGCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCa  <  1:188247/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  ATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCT‑GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCA  >  NC_000913_3_pae_tpiA/4139291‑4139578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.