New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2530197 =	29 (1.170)	3 (0.130)	3/268	3.1	9.2%	intergenic (‑21/+50)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
	?	NC_000913_3_pae_tpiA	2530218 =	32 (1.370)					intergenic (‑42/+29)	ligA/zipA	DNA ligase, NAD(+)‑dependent/FtsZ stabilizer
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCA  >  NC_000913_3_pae_tpiA/2530070‑2530352                                                                                                                                                     |                                                                                                                                         tCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTc                                                                                                                                          >  1:420607/1‑149 (MQ=255)         aGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAgagga                                                                                                                                  >  2:12589/1‑149 (MQ=255)         aGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAgagga                                                                                                                                  <  1:361051/149‑1 (MQ=255)             acGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGTCAAGCGGGGGTTCGAAGAGGAGTTa                                                                                                                              >  2:328430/2‑149 (MQ=255)                          gcgcATCCATCACATGATAAAGTTATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAgt                                                                                                                 <  1:63372/149‑1 (MQ=255)                                 cgtccactGATAAAGTTACTCATGATGGCGAAGCGTCGTTCGCAGGTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAg                                                                                                         <  2:327211/142‑1 (MQ=255)                                 catcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGGGTTATTTTGCCTTAAGTGT‑ATCAg                                                                                                         >  1:267280/1‑149 (MQ=255)                                  atcACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAgg                                                                                                        <  2:271385/149‑1 (MQ=255)                                     acaTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCAACAAGCGGGGCTTCAAAGAGGAGTTAATTTGCCTTCAGTGT‑ATCAAGCGt                                                                                                     >  1:52340/1‑149 (MQ=255)                                       aTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTg                                                                                                   >  1:352983/1‑149 (MQ=255)                                       aTGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTg                                                                                                   <  1:12589/149‑1 (MQ=255)                                           tAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGt                                                                                               <  1:196983/149‑1 (MQ=255)                                               gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCttt                                                                                           <  2:331061/149‑1 (MQ=255)                                               gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCttt                                                                                           <  2:136444/149‑1 (MQ=255)                                                           ggCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGgat                                                                               <  2:300809/149‑1 (MQ=255)                                                                    tcgtTCGCAGTTCTGTCTGTGGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGTTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                      <  2:278831/149‑1 (MQ=255)                                                                    tcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTGATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                      <  1:120741/149‑1 (MQ=255)                                                                         cGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACt                                                                <  2:352983/149‑1 (MQ=255)                                                                          gCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCCGGCGCATTCCGCTTGGACTTCGCGGATGATGTCCTGGT‑ACTc                                                               <  1:416993/149‑1 (MQ=255)                                                                                             ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCttt                                                                                           >  2:328499/1‑103 (MQ=255)                                                                                             ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCttt                                                                                           <  1:328499/103‑1 (MQ=255)                                                                                                         cATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGGGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGgcgc                                >  1:397485/1‑149 (MQ=255)                                                                                                          atatCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCt                               >  1:138507/1‑149 (MQ=255)                                                                                                                     tCACTGCTAAAAACCCCCGACAAGCGGGGGGTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGc                    <  1:125192/149‑1 (MQ=255)                                                                                                                       aaTGCTAAAACCCCCCAACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCAc                  <  2:264130/149‑1 (MQ=255)                                                                                                                         tGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGa                <  2:220622/149‑1 (MQ=255)                                                                                                                            tAAAAACCCCCGACAAGCGGGGGTGCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAAGCGTTTGCGTATTTGACTTCGCGGATGATGTACTGGT‑ACTCGCGCAATTTCTGCGTAGCCATCATGCCGCACTGCTCGTCCAGCACGccac             >  2:185056/1‑149 (MQ=255)                                                                                                                                accacccGGCTAGCGGGGGTTGGAAGCTGAGTTAATTTGCCTTAA‑TGTGAGCAGGCGTTGGCGTCTTTGACTTCGCGGTGGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATGATACGGCGCGGATCGTCAAGGGCGAGACCGcc         <  2:189669/145‑1 (MQ=255)                                                                                                                                     ccGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGa                <  2:190960/137‑1 (MQ=255)                                                                                                                                     ccGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGa                >  1:190960/1‑137 (MQ=255)                                                                                                                                     ccGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACtt    >  2:124888/1‑149 (MQ=255)                                                                                                                                       gACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGATCGCTTG‑ATAGTTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCa  >  1:310601/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                         TCGTATTCAGCGTCGGGAATTTCCGGCGCATCCATCACATGATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGT‑ATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGT‑ACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCA  >  NC_000913_3_pae_tpiA/2530070‑2530352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.