Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA482,2300GA96.9% 87.7 / NA 33P725L (CCG→CTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (19/12);  minor base C (1/0);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCG‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_pae_tpiA/482089‑482375
                                                                                                                                             |                                                                                                                                                  
tAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGACTGCGCTTTTTACTGGCCAATATCAGTACTGAATTGCA‑GGGTATc                                                                                                                                            >  2:108835/1‑149 (MQ=255)
           cacaCGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTGCAGGTCGATGTCAGTCTTAAACTGCA‑GGGTATCTTCCAGACCGt                                                                                                                                 >  1:482171/1‑149 (MQ=255)
            acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGtt                                                                                                                                >  2:211985/1‑149 (MQ=255)
             cacGGCCGCGGTCGATAAAAGCGTTCACATAGCTGGCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGTTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGttt                                                                                                                               <  2:581046/149‑1 (MQ=255)
              acGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTg                                                                                                                              <  1:26666/149‑1 (MQ=255)
                gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGa                                                                                                                            <  1:251052/149‑1 (MQ=255)
                          gATAAAGTCGTTCACATAGCTGCCGCACCATGCAGCGCCCAGAGTGGTGTTAAGGTCGTTGATAGGAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTCAACTGCC‑GGGTATCTCCCAGACCGTGTGCACGTCCGCTgg                                                                                                                  >  2:174757/1‑149 (MQ=255)
                            tAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGACTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATGTCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGCTc                                                                                                                >  2:588682/1‑149 (MQ=255)
                              aaGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCaa                                                                                                              <  1:211985/149‑1 (MQ=255)
                                    ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                                                        >  1:540573/1‑149 (MQ=255)
                                       acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACGGTTTGGACGTACGCTGGTCAACatagaagg                                                                                                     >  1:254846/1‑144 (MQ=255)
                                         aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGATGTACGCTGGTCAACACATCAgtgt                                                                                                   >  2:239339/1‑149 (MQ=255)
                                         aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                                   >  1:331050/1‑149 (MQ=255)
                                               gccgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCg                                                                                             <  1:502516/149‑1 (MQ=255)
                                                ccgccCCATGCAGCGCCCAGAGTGGCGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCgc                                                                                            <  1:333326/149‑1 (MQ=255)
                                                   ccccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTTGTCTTTATCAATCTTAAACTGCA‑GGCTATCTTCCAGACCGTTTCGACGTACGCTGCTCAACATATCACCGTCCTTCcctcc                                                                                         >  2:369485/1‑147 (MQ=255)
                                                     acATGCAGCCGCCAGGGTTGTGTTAATGTCGGTGATAGAAACACCCAGCGCCTGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGCACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCtt                                                                                       <  2:404506/148‑1 (MQ=255)
                                                           aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                                 <  1:445230/149‑1 (MQ=255)
                                                                          gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgcg                                                                  >  1:48181/1‑149 (MQ=255)
                                                                          gtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGCTTGGACGTACGCTGGTCGACATATCAGGGTGCTTCGCTGCTTCGGCAAGCAACTGGTTAcgcg                                                                  >  1:339432/1‑149 (MQ=255)
                                                                            tAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGcc                                                                >  1:14073/1‑149 (MQ=255)
                                                                             aaTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTAAGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt                                                               <  2:367532/149‑1 (MQ=255)
                                                                              aTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCACCTGGTTACGCGCCTg                                                              >  2:571663/1‑149 (MQ=255)
                                                                               tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGAGCCTGa                                                             >  1:163637/1‑149 (MQ=255)
                                                                                       aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                     >  1:602341/1‑149 (MQ=255)
                                                                                                        cTGCGCTTTTTCCTGGTCGATATC‑ACAACAACTTGAGAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAg                                    >  2:598780/1‑149 (MQ=255)
                                                                                                                     tGGTCGATATCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                       >  1:189548/1‑149 (MQ=255)
                                                                                                                           atatCAATCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                 <  2:163637/149‑1 (MQ=255)
                                                                                                                                  tCTTAAACTGCA‑GGGTATCTTCCGGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCAAGCCTGGTCAATCAGCtcaaagtcaaag          >  2:317792/1‑149 (MQ=255)
                                                                                                                                  tCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAGGGCCAGCCTGGGCAATCAGCtcaaagtcaaag          >  1:148983/1‑149 (MQ=255)
                                                                                                                                  tCTTAAACTGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag          <  2:167391/149‑1 (MQ=255)
                                                                                                                                        aCTGCA‑GGGAATCTTCCAGACAGATTGGACGTACGCTGGCAAACAAACCACGATGCTCCACCAGCTGGACGTGCAGCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCACGCTCGCCAACCAGCCCAAAGTCAAACCCgctc    >  2:132116/1‑146 (MQ=255)
                                                                                                                                          tGCA‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc  <  1:409242/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCG‑GGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGC  >  NC_000913_3_pae_tpiA/482089‑482375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑