| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,544,021:1 | +A | 78.1% | coding (40/1389 nt) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,544,021 | 1 | . | A | 78.1% | 74.4 / 18.7 | 32 | coding (40/1389 nt) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base . (4/3); new base A (11/13); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCG‑C‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAG > NC_000913_3_pae_tpiA/1543874‑1544158 | ttgtATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATGTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTc‑gcga < 2:72740/146‑2 (MQ=255)ttgtATTGAGATTAACGGTAACGGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATAGCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTc‑gcga < 2:72833/146‑2 (MQ=255) ttGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAAGCAAGAAGGAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATTTTTTCCTTTATTTTCCCACAAGGCCGGATTTTCTGGTTTCCAGTC‑CCG‑A‑aaa‑aa > 2:557105/1‑148 (MQ=255) tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTtc > 1:506092/1‑149 (MQ=255) gcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCG‑A‑AAACAGATAACGACTATTTTTCTCa > 1:666407/1‑149 (MQ=255) tAGCATCAAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGCTGACTTCGAGCAAGAAGTTTTCCTTTATTTTCCCATAAGGCCGGATTTTCTGGTTTCCATTG‑GCGTA‑AAA‑AGATAACGACTATTTTTCTCATCTTGc > 2:708838/1‑149 (MQ=255) gtgtCCAGACGCAGATGTCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTACTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAg < 2:281470/146‑1 (MQ=255) tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGc > 2:603081/1‑149 (MQ=255) cAGACACAGAGGGGAAGAAGGAGACAACGGACGGATCTCCAGAGATTTCAGCGAGCAATATGTTTTCCTTTATTTTACCAGAAGGCCGGATTTTCTGGATTCCAATC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCa < 2:282663/149‑1 (MQ=255) aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCATTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTACAGTC‑GCGAA‑AAA‑AGATAACCACTATTCTTCTATTTTTGCAGTGCCat > 2:426551/1‑149 (MQ=255) caGAAGGCAAGAAGGAGACAACTGACGGATAACCAGAGATTTCTTCGAGCAATATGTTTGCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt < 1:113996/149‑1 (MQ=255) aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca < 1:133968/149‑1 (MQ=255) tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGCTTTTCTGGTTTCCAGTC‑TCG‑AAAAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGACATATTGTTCCTCACATGCACACTATGGTAATGa > 2:162538/1‑149 (MQ=255) cAGAGATTTCTTCGAGCAATATGTTTTCCTTTCTTTTCCCGGAAGGCAGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGTATATTTTTCTCATTTTGCAGTGCACTATTGTTACTCTCATGCACACATATGTGATAaaaataat > 2:551858/1‑148 (MQ=255) aagagATTTCTCCAAGCAACATGCCTTCCCTTCTTTACCCAGAAGACCCCATTATCTAGTTACCACAC‑AAGAA‑AAA‑AGAGAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACCTGCACACATTGGTAATGAAAAAAAg < 2:528636/148‑1 (MQ=255) agagCTTTCCTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCATATGCACACATTGGTAATGAAAAAAAGa < 1:87248/149‑1 (MQ=255) gagaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc > 1:135646/1‑149 (MQ=255) tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 1:494769/149‑1 (MQ=255) gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAgg > 1:129884/1‑149 (MQ=255) gAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAgg > 1:130389/1‑149 (MQ=255) cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa > 2:662880/1‑149 (MQ=255) tatGTTTGCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCG‑AAAAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc < 1:169927/149‑1 (MQ=255) tttCCTTTATTTTCTCGGAGGGCCGGATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTGCCTCACATGCACACAGTGGTAATGAAAAAAAGACAAAACACGGGGTAAGGCGCAAt < 2:512371/149‑1 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCG‑AAAAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGccg > 1:55246/1‑148 (MQ=255) aTTGTCCGAGAAGGACGGTTTTTCTGGTTTCGAGTC‑GGGAA‑AAA‑AGATAACGACGATTGTTCTGATTTTGCAGTGCCATATGGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtt < 2:116081/149‑1 (MQ=255) gCCGGGTTTTCTGGTTTCCAGTCGGTA‑A‑AAA‑AGATAACGACTATTTTTCTCATTTTGTAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAgg < 2:61135/149‑1 (MQ=255) taggttttcCTGTTTTCCAGTCTACA‑A‑AAA‑AGACAACGACTATGTTTCGAAATTTGCAGTGCCATTTTGTTCCTCACATGCACACATTGGTAAGGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGa < 2:291915/144‑1 (MQ=255) ggATTTTCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaaaaaag > 1:145301/1‑144 (MQ=255) ggATTTTCCGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTCGAATTAAGGagg > 1:91114/1‑147 (MQ=255) ttCTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTg < 1:588047/149‑1 (MQ=255) cTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg < 1:475788/149‑1 (MQ=255) cTGGTTTCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAAGAATTggg > 2:293888/1‑149 (MQ=255) cTGGTTTCCAGTA‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg < 2:135646/149‑1 (MQ=255) ttCCAGTC‑GCGAA‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGATATTAGAATTAAGGATGAATTGGGTGAAg < 1:603081/149‑1 (MQ=255) | TTTTATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTC‑GCG‑C‑AAA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAG > NC_000913_3_pae_tpiA/1543874‑1544158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |