Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	100.0%	95.4 / NA	36	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/14);  total (22/14)

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263952‑264238                                                                                                                                                   |                                                                                                                                              cGGGCTCCCAGGCGCAGTTTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTCCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                             <  1:366913/149‑1 (MQ=255) cGGGCTCCCAGGCGCAGTGTAATCTCCGTCTGCAGGCCCTTTGTAGGGTTGCTCATTACAGTGCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                             <  2:604337/149‑1 (MQ=38)    gCTCCCAGGTGGTGGTTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGGGATGCTCATTAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                                                                                                          <  1:723918/149‑1 (MQ=255)         cAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGGCATGACGGCGTTCGGGGTATACAGCTAGGACtaagg                                                                                                                                     >  1:637113/1‑145 (MQ=255)              gcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                                                                                                >  1:956328/1‑149 (MQ=255)                 gtgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatatac                                                                                                                             >  2:715415/1‑147 (MQ=255)                    tAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTAGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGa                                                                                                                          >  1:8514/1‑149 (MQ=255)                           ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCCGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACTTccc                                                                                                                   >  1:414611/1‑149 (MQ=255)                                    gCCTCTGGTAGGGTTGCTCATTACAGTTCTTCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                          <  1:111826/149‑1 (MQ=255)                                      cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                        <  2:361799/149‑1 (MQ=255)                                      cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAACGCTGCGACTCCGGCGATATGCGGGACGTCCCTGCCGGAGTaa                                                                                                        >  2:793442/1‑149 (MQ=37)                                           gTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                                         <  1:810983/127‑1 (MQ=255)                                           gTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCAGaa                                                                                                                         >  2:810983/1‑127 (MQ=255)                                                       aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCggg                                                                                       >  1:145849/1‑147 (MQ=255)                                                        ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTATTGACAGACGCTAcccttc                                                                                      >  1:387662/1‑145 (MQ=255)                                                            aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAAAGACGCTACCGTGC‑CACt                                                                                 >  1:369036/1‑149 (MQ=255)                                                                 tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTTCCTGCCGGCGCAATGACAGACCCAGCCTTGC‑CCCTGGTAt                                                                            >  1:870858/1‑149 (MQ=255)                                                                   cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                        >  2:598886/1‑120 (MQ=255)                                                                   cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                        <  1:598886/120‑1 (MQ=255)                                                                    aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGAAACGTCCCTGCCGGTGTAATGACAGACGCTACTCTGC‑CACCGGTATTTg                                                                         >  1:119046/1‑149 (MQ=25)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGA‑CACTGGTATTTGCCGGTGCAGTa                                                              >  1:426848/1‑149 (MQ=255)                                                                                  aGGGTGATGCTCATCAGGCACGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTCCCGTGC‑CACTGGTATTTGCCGGTGCCGCAGCg                                                           >  2:25662/1‑149 (MQ=21)                                                                                              atcaGTCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCAAACAACTATGT‑TCGGTGCAGTAGCGAAAGATTCGGGa                                               <  1:510517/149‑1 (MQ=18)                                                                                                  ggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCa                                           >  1:36493/1‑149 (MQ=255)                                                                                                    caggTATAGACGCCATTGCAGTCCTGGCTGCGCTCGGCATATACCGCTAGGACTCCTGCGATATCCGGAACGTCCCTGCCAGTGTAATGACAGACGCTACCTTGC‑CACTTGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAgg                                         <  2:425555/149‑1 (MQ=17)                                                                                                        tataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGATACCGTGC‑AACTAGTATCTTCGGTTACAGGAGCGAAAGATTTGGGACTCAGGATGc                                     >  1:545453/1‑149 (MQ=17)                                                                                                          taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTTGCCGTAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTg                                   <  1:501196/149‑1 (MQ=18)                                                                                                          taGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAAAGAAGCTACCGTGC‑CAATGGTATTTGCCGGTGCAGTAGCGAAAGATTCGCGACACAGGGTACTg                                   >  1:376725/1‑149 (MQ=18)                                                                                                          taGGGCCCATTGCGGTCCTGGCGGCGAACAAACTATACCGCTAGGACTCCGGCGATATCAGGGACGTGCCTGGCGGTGTGATGGCAGACGCTGCCGTGC‑CACTGGTATTTGCCCGTGCAGGAGCGGAAGATTCGGGACTCCGGATGCTg                                   >  2:354499/1‑149 (MQ=255)                                                                                                             ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTCCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                                >  2:255011/1‑149 (MQ=255)                                                                                                                      cGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGt                       >  2:277005/1‑149 (MQ=255)                                                                                                                              gcggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTTccc               >  1:932346/1‑149 (MQ=255)                                                                                                                                 gcgTTCGTCGGATACCGCTAGGACTCCGGCGATATCCGTAACGTCCCTGCCGGTTTAATGACAGACGTTGCCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCTAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGc            <  2:352573/149‑1 (MQ=17)                                                                                                                                      cGGCGTATCCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa       <  1:683906/149‑1 (MQ=255)                                                                                                                                       ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTAc      <  2:760038/149‑1 (MQ=255)                                                                                                                                           tatcCCGCTAGTCCTCCGGCGATATTGTGAACGTCGCTGCCGGTTGAATGACAGACGCTACCGTGC‑TACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAt  <  1:599917/149‑1 (MQ=14)                                                                                                                                                   |                                                                                                                                              CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGC‑CACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263952‑264238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑