New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3123452 =	37 (0.940)	5 (0.130)	5/270	4.7	11.9%	coding (354/2172 nt)	glcB	malate synthase G
	?	NC_000913_3_pae_tpiA	3123480 =	39 (1.050)					coding (326/2172 nt)	glcB	malate synthase G
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAA  >  NC_000913_3_pae_tpiA/3123337‑3123627                                                                                                                                                |                                                                                                                                                     cGCTGACCATCACCCCTTCCTGCGGGATGATGTCGCTGCCGTATCACGCATCGTACAGTGTGCCCGAGCGAGCGTTCGCCGCGTTCAGCGCGTGGCGGGCGTTCATTGCCGGAATCACCAGCTGCGGCGCCGCCTGGCTCGTGATTTCg                                                                                                                                                 <  2:548955/149‑1 (MQ=255)    tGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGTTGATTTCGCTg                                                                                                                                              <  1:436993/149‑1 (MQ=255)             ccccTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                                                     <  2:561938/149‑1 (MQ=255)                cTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCCGTCTGGTGATTTCGCTGTCCATGCCCGTg                                                                                                                                  >  2:319546/1‑149 (MQ=255)                cTTCCTGCGGGATGATGGCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTg                                                                                                                                  <  2:379928/149‑1 (MQ=255)                 ttCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGAGAGCCACAGCGAGAGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGACCCACCAGCTGCGGCCCCGCCTGGCCGGTGCTTTCGCCGTCAATGCCCgggg                                                                                                                                 >  2:298271/1‑149 (MQ=255)                         ggATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                                                     >  2:600631/1‑137 (MQ=255)                         ggATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGccc                                                                                                                                     <  1:600631/137‑1 (MQ=255)                              atgTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CAc                                                                                                                   <  1:409500/149‑1 (MQ=255)                                   gCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGCGCGTTCCCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGGTTCCACCGT‑CAAGCGCt                                                                                                              >  1:298402/1‑149 (MQ=255)                                    cTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTc                                                                                                             >  2:444087/1‑149 (MQ=255)                                     tGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTcc                                                                                                            >  1:245272/1‑149 (MQ=255)                                               cGCATCGTACCGTGTGCCCCAGCGAGCGTTCGCCGCGTTCCGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCCGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGTTTGcttca                                                                                                  <  2:84554/149‑5 (MQ=255)                                               cGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCa                                                                                                  >  1:258150/1‑149 (MQ=255)                                                     gTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCCCCGGTTGCGGCACCAGGt                                                                                            >  2:240939/1‑149 (MQ=255)                                                           tGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATGTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCa                                                                                      <  2:502361/149‑1 (MQ=255)                                                                 ccAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCAc                                                                                >  1:630937/1‑149 (MQ=255)                                                                      gagcgTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGG‑CACGACCTTACACTCTCTCCGGTTGCGGCACCAGGAAGCCCAGTTCACGCAgg                                                                           >  1:402125/1‑149 (MQ=255)                                                                          gTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGGGCTCCGGGTGCGGCCCCCGGTGGCCCACTTCACGCAGGAAAg                                                                       >  2:285902/1‑149 (MQ=255)                                                                              gccgcGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCACGAAAGAttt                                                                   >  1:103804/1‑149 (MQ=255)                                                                               ccgcGTTCAGCGCGTAGCGGGCGGTCATTGCCGGGACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCGCCGT‑CACGCGCCCCGGTTGCGGCACCAGGTAGCCAAGTTCACGCAGGCAAGAtttg                                                                  >  2:368237/1‑148 (MQ=255)                                                                                  cgTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAg                                                               >  1:403289/1‑149 (MQ=255)                                                                                   gTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAgg                                                              <  2:658812/149‑1 (MQ=255)                                                                                   gTTCAGCGCGTAGCGGGCGTTAATTGCCGGAACCACCAGCTGCGGCCACGACTGGGTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCCACAGGTAGCACAGTTCACGCGGGAAAGGTTtatgag                                                              >  2:221535/1‑146 (MQ=255)                                                                                   gTTCAGCGCGGAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCAACAGGTAGCCCAGTTCACGCAGGAAAGATTTATAgg                                                              <  2:238759/149‑1 (MQ=255)                                                                                                 gggCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCtttt                                                <  1:375298/149‑1 (MQ=255)                                                                                                   gCGTTCCTTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTAc                                              <  1:436750/149‑1 (MQ=255)                                                                                                     gTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCg                                            <  1:473977/149‑1 (MQ=255)                                                                                                            gCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATCGGCCGCTTTATCTTTTACCGGCCCCgg                                     >  2:159359/1‑149 (MQ=255)                                                                                                             ccGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtat                                                                 >  2:429449/1‑120 (MQ=255)                                                                                                             ccGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtat                                                                 <  1:429449/120‑1 (MQ=255)                                                                                                                  accaccAGCTGCGGCCCCGCCTGGCCGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATtgct                               <  1:127959/149‑1 (MQ=255)                                                                                                                       catcTGCGGTGCCGTCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCCCCGG‑CTCGCTCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGAt                          <  1:336372/146‑1 (MQ=255)                                                                                                                            gccgCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CCCGCGCTCCGGTTGCGGCACCAGGTAGTCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCAc                     <  2:577045/146‑1 (MQ=255)                                                                                                                              ggCCCCGCCTGGCTGGTTATTTCGCTGTCGATGCCCTTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATTGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACtc                   <  2:210544/149‑1 (MQ=255)                                                                                                                                ccccGCCTGGCTGGTGGTTTCGCTGTCAATGGCCGTGGGTTCCACCGT‑CACGCGCTGCGGTTGTGGCACCAGGTGGTCTAGTTCACGGAGGAAAGATTTATAGGCCGGTTTATCTTTTTCCGACGCCGGATTGCTGCGATGcccgtctt                 >  2:228338/1‑143 (MQ=255)                                                                                                                                ccccGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CGCGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCTATGCCACgcat                 >  2:575642/1‑149 (MQ=255)                                                                                                                                    gCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGCTGCCCCTCATCAAg             >  2:189802/1‑149 (MQ=255)                                                                                                                                    gCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAg             <  2:258150/149‑1 (MQ=255)                                                                                                                                            gttGATTTCGCTGTCACTGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGTAAGATTTCTATGCCTCTTTATCTCTTACCGGCCCCGGATTGCTGCGATGCCACACATCAAGTGCTGCCt     <  2:568523/147‑1 (MQ=255)                                                                                                                                               gATCTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCCCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGaa  <  1:645/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGT‑CACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAA  >  NC_000913_3_pae_tpiA/3123337‑3123627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.