| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 2,679,525 | G→T | A1074D (GCT→GAT) | yphG ← | DUF4380 domain‑containing TPR repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 2,679,525 | 0 | G | T | 96.0% | 70.7 / NA | 27 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (13/11); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGG > NC_000913_3_pae_tpiA/2679385‑2679667 | ttttATTAACAACGGCTTATTCTAATTATATTGTGATGAGCGGCAGCGCATGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGAGAAAAATCCCGCCTTCGGCCAACCCCGATTa > 2:23108/1‑149 (MQ=255) aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCa > 2:353908/1‑149 (MQ=255) ttCTTATTAGTTTGTTATGTTCTGCAGCGAGTGCCGCTCATATATTACAGTTAATAGGGTTTAGTGAATGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAgcgc < 2:168597/149‑1 (MQ=255) cTAATGATTGTGTTAGTAGCGTCAGCGCGTGCCGCACAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATCATGCAGTTAGAGGCCAAACCGGATTAATCGCCG‑TCAATTCAGCGCGa > 2:366544/1‑149 (MQ=255) tAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAg > 2:396966/1‑149 (MQ=255) tttGTGATGAGTGGTAGCGTGTTATTCGCTGATATTATATTTAAAATGGTTTAGTTAACGCGTTTAAAAATAAAAGGCTTCTCGGTGGTGAAGAATGCCGCTTTCGGCCAATCCGGATTATTCGCCG‑TCAATTCAGCGCGAGCAGAcgc < 2:492032/149‑3 (MQ=255) tgaGCGGAAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTGAAAAATAAATGGCATCACGATGGTGAATAATTACGCTTTCGGCCAAGCGGGATACATCGCCT‑GCGATGTCGCGGGAGCGGCCTCATGTTgc > 2:86923/1‑149 (MQ=255) gCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTCAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc > 1:448791/1‑149 (MQ=255) aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCACCCTCCCCGAGTCCCGCGGACGCCAgac > 2:275689/1‑147 (MQ=255) ttAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc < 1:300510/149‑1 (MQ=255) ttAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc > 1:332003/1‑149 (MQ=255) aTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGcc > 1:319809/1‑149 (MQ=255) aTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGGGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAATCCCGCGGACGCCAGCATCgac > 2:393887/1‑147 (MQ=255) ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccacca > 2:198683/1‑149 (MQ=255) aatccgGTTAAAAAAAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TAAATTCAGCGCGAGCAGACTCATATTGCGCACCCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtt < 2:165180/145‑1 (MQ=255) ggCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtgttg < 1:396966/149‑1 (MQ=255) gCATCACGGTGGTTAATAATGCCGCTGTCGGCCAATCCGGACTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCGGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtgttgt < 1:493139/149‑1 (MQ=255) cATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTa > 2:203665/1‑149 (MQ=255) aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaa < 1:251735/149‑1 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCg < 1:515948/43‑1 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCg > 2:515948/1‑43 (MQ=255) gtggtgAATCATGCCGGTTTCGGCCAATCCGGCTTACTCCATGCTCCA‑TCAGCTCGAGTCGACTCAACTTTCGTTACTCCCCCAAAATGCCCCGACGTCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGcc < 1:122650/149‑1 (MQ=255) gtggtgAATAATGCCGCTTTCGGTCAATCCGGATTAATCGCCG‑TCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGcc < 2:304783/149‑1 (MQ=255) atGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTCTAAATCGCCATTCAGCGac > 1:165320/1‑149 (MQ=255) tGCCGCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGaca > 1:214757/1‑149 (MQ=255) gCTTTCGGCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 1:504380/149‑1 (MQ=255) ggCCAATCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGTAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAgg > 1:359694/1‑149 (MQ=255) | TTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCG‑TCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGG > NC_000913_3_pae_tpiA/2679385‑2679667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |