| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 482,230 | G→A | P725L (CCG→CTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 482,230 | 0 | G | A | 95.7% | 62.9 / NA | 23 | P725L (CCG→CTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/10); minor base C (0/1); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGG > NC_000913_3_pae_tpiA/482087‑482371 | catAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCTTGGTCGATATCAATCTTAAACTGCAGGGTa > 1:146995/1‑149 (MQ=255) ttcACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTACAGAGTATCTTCCAGAcc > 2:123647/1‑149 (MQ=255) cacGACCGTGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGttt < 2:285445/149‑1 (MQ=255) cgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCATAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTAc < 1:7899/149‑1 (MQ=255) gTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTGATGTCGTTGATAGAAACACACAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTCAAGGGTATCTTCCAGACCGTTTG‑GAcattcag > 2:497242/1‑143 (MQ=255) ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATc > 1:412661/1‑149 (MQ=255) gccgccCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCg > 2:492481/1‑149 (MQ=255) aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa < 1:492481/149‑1 (MQ=255) ttAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTACACTGCAGGGTATCTTCCAGACCGTCTGACAGCAATACGGATTAGCATA‑CAGGGTTCTTCTCTCCTTCTGCGCGCAACTGGTTACgcgc > 2:106531/1‑149 (MQ=255) ggcGTTGATAGAAACACCCAGCGCCTGCGCTTTCTCCTGTTCTAGTTCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGATTATGCAAGCATCTGGTCACGCGCCTGAg < 2:232702/147‑1 (MQ=255) ttGATAGAAACCCCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAg < 2:447219/149‑1 (MQ=255) aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAGACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:51525/1‑149 (MQ=255) acacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTg > 1:220060/1‑149 (MQ=255) cacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa < 1:497242/149‑1 (MQ=255) gcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg > 1:205302/1‑149 (MQ=255) cTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCCGCCTGGCCAATCa > 2:194860/1‑149 (MQ=255) cTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa < 1:442722/149‑1 (MQ=255) ggTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCACGGCTAGCCTGGTCAATCAGc < 2:187940/149‑1 (MQ=255) gATATCAATCTTAGACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 2:51525/149‑1 (MQ=255) atacCAATCTTAAACTGCCGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa < 1:123471/149‑1 (MQ=255) atCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt > 2:15926/1‑149 (MQ=255) tCTTAAACTGCAGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 2:339732/1‑149 (MQ=255) aaaCTGGAGCGTATCCTCCAGACCGTTTG‑GACGTACGCGTGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGCGTCAGGCTTGCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg < 2:457085/149‑1 (MQ=255) | CATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTG‑GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGG > NC_000913_3_pae_tpiA/482087‑482371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |