Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,123,229	C→A	L15L (CTG→CTT)	yceB ←	lipoprotein, DUF1439 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,123,229	0	C	A	100.0%	100.6 / NA	31	L15L (CTG→CTT)	yceB	lipoprotein, DUF1439 family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/14);  total (17/14)

GTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAA  >  NC_000913_3_pae_tpiA/1123089‑1123373                                                                                                                                              |                                                                                                                                                 gTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGAAGGCGACGGGCT‑ACCACTGGAGGTGGTGTTGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACt                                                                                                                                          >  1:539527/1‑149 (MQ=255)       tttGTCAGCACAATATGTGCGTCAGCCACGCCGGGTAAACCGAGATCTTTTGAGAAATTATTATGTTTCGCAAGCGCCTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGAt                                                                                                                                    <  2:318223/149‑1 (MQ=255)           tCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAAt                                                                                                                                <  1:502895/149‑1 (MQ=255)            cAGAACAAGATGGGCGTCAGCAACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTCAGTGTGTATTGGGTTAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATg                                                                                                                               <  2:382554/149‑1 (MQ=255)              gAACAATATGGGCGTCAGCCACGCCCGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGAAGGTGTATTGGGTGAGCTGATTACAGCCCACGAGAAGGCCACTGACGATCAAAGCa                                                                                                                             >  2:264823/1‑149 (MQ=255)               aaCAATATGGGCGTCACCCACGCCGGGTAAACCGATACCTTTTGAGAAATTATTATGTTACGCTAGCGACTGGTTAAATTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGACAAGGCCACTGACGATCAATGCAg                                                                                                                            <  2:530760/149‑1 (MQ=255)                        ggCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGATGTATTGGGAGAAATGATTACAGCCAACGAGAAGGCCACTGAAAATCAATGCAGCGGCAAATa                                                                                                                   >  1:743/1‑149 (MQ=255)                               gCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACtt                                                                                                            <  1:112366/149‑1 (MQ=255)                               gCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACtt                                                                                                            <  1:62934/149‑1 (MQ=255)                                      cGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATg                                                                                                     <  1:26815/149‑1 (MQ=255)                                       gggTAAACCGGTATCTTTTGAGAAATTAGTATTTTTCGCAAGCGACTTGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATgg                                                                                                    <  2:34196/149‑1 (MQ=255)                                                      ttttGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGt                                                                                                          >  1:479137/1‑128 (MQ=255)                                                      ttttGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGt                                                                                                          <  2:479137/128‑1 (MQ=255)                                                            gaAATTATTATGTTTCGCAAGCGACTGGTTAATCTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAAGTTGTTCATGGTAGTCCTCGACATCAAATCtc                                                                               >  2:328547/1‑148 (MQ=255)                                                                 tattatGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAACACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCa                                                                          >  1:502390/1‑149 (MQ=255)                                                                   ttatGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAACCTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAat                                                                        >  1:371133/1‑149 (MQ=255)                                                                        tttCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTAGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGGTCATGATAGTCCTCGACATGCACTCTGCGTCAATATcat                                                                   >  2:180709/1‑147 (MQ=255)                                                                                       gTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCAt                                                    >  1:461054/1‑149 (MQ=255)                                                                                         tAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCAAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCAtgt                                                  <  2:421310/149‑1 (MQ=255)                                                                                            tttCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATCCAGCGGCAAATAAAAACTTGTTCCTGGTAGTCCTCGCCATGAAACCAACGGTCATATCCTGACACAACGCAGCAtgccac                                               >  2:158394/1‑145 (MQ=255)                                                                                                ttG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCa                                           >  1:358570/1‑149 (MQ=255)                                                                                                 tG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAg                                          <  1:476625/149‑1 (MQ=255)                                                                                                     tgggTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGa                                       <  1:370276/147‑1 (MQ=255)                                                                                                      cGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGCGTCACGAGCGac                                      >  2:185340/1‑148 (MQ=255)                                                                                                                   ttGGGTGAGTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCcagca                         >  1:389552/1‑149 (MQ=255)                                                                                                                           gTTGATTACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAAc                 >  1:397844/1‑149 (MQ=255)                                                                                                                            ttGATTACAGCCAACGAGAAGGCCACGGACGATCACTGCAGCGGCAAATAAAAACATTCTCAGGGTATTCCTCAACATGAAATCTGCGTCAATATCCTGAAACAACGCAGCAGAAGTCAACCACGATAAACACGCCACGCGAAAAAACt                >  2:552995/1‑149 (MQ=255)                                                                                                                                ttACAGCCAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGATAAAACTGaca            <  1:288331/149‑3 (MQ=255)                                                                                                                                      ccAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAAcggcg      >  1:6043/1‑149 (MQ=255)                                                                                                                                       cAACGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAAcggcgg     <  1:440548/149‑1 (MQ=255)                                                                                                                                          cGAGAAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCaa  >  1:415254/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                 GTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTG‑TTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCCTCGACATGAAATCTGCGTCAATATCCTGACACAACGCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAA  >  NC_000913_3_pae_tpiA/1123089‑1123373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑