New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3250260	34 (0.930)	4 (0.120)	4/270	4.6	11.2%	coding (184/300 nt)	yqjK	uncharacterized protein
	?	NC_000913_3_pae_tpiA	= 3250270	31 (0.900)					coding (194/300 nt)	yqjK	uncharacterized protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCGGCCTTA  >  NC_000913_3_pae_tpiA/3250112‑3250407                                                                                                                                                      |                                                                                                                                                    gcacgtGCGTAGCCAGATCCAGCAACAACGGGTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGa                                                                                                                                                     <  2:97867/144‑1 (MQ=255) gaaaCTGCGTAGCCAGAAGCAGCAACAACGGCTGGATCTATCCGCCATTCGTCGTGAATGGCTGGAGACAACAGGCGCTGACGATCGTCGCTGGAATATGCTGCTGAGTCTGCGCTCCTGGGCTCTGGTTGGCAGTAG‑CGTGATGGCGa                                                                                                                                                     <  1:490600/147‑1 (MQ=255)        cTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGTGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGAc                                                                                                                                              <  2:562664/149‑1 (MQ=255)         ttAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCAAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGAAATATGCTGGTAAGTCTGGGCTCCTGCGCGCCCCCTGACA‑TAGACGTGATGGCGGTCTGGACg                                                                                                                                             >  2:585639/1‑149 (MQ=255)              cAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCGTGGGCGCAGGTTGGCAGTAG‑CGTGATGGCGAGCTGGCCGATGCg                                                                                                                                        >  2:134590/1‑149 (MQ=255)               aGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGGTCGGCGCGTGAATATTCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGc                                                                                                                                       <  2:633733/149‑1 (MQ=255)                       caacaaCGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGTCGATTCGCCATCCTaa                                                                                                                               <  1:561717/149‑1 (MQ=255)                        aacaacGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAat                                                                                                                              <  2:142159/149‑1 (MQ=255)                              ggCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCTCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCTCCATCCTAATATGCTg                                                                                                                        <  1:560094/149‑1 (MQ=255)                              ggCTGGACCTTTCCGCCAGTCGTCGTGAATCGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTg                                                                                                                        <  1:433675/149‑1 (MQ=255)                                       tttCCGCCAGTCGTAGTGAAGGGCTGGAGACAACAGGCGCGTACGATCGGCGGTAGAATATCCTGCTACGACGGCGCGCTTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTgg                                                                                                               <  2:394052/149‑1 (MQ=255)                                                    tcgtGAATGGCTGGAGACAATAGGCGCTTAGGATCGTCGCTGGAGTATGCTGCTAAGTCTGCGCTGCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGTACGATTCGCAATCCTACTAGGCTGGTCAGCTGGGCGCGACGTGGtt                                                                                                  >  2:325597/1‑149 (MQ=255)                                                         aaTGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCAGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGc                                                                                             <  1:425829/149‑1 (MQ=255)                                                            ggCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCGTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTa                                                                                          <  2:299545/149‑1 (MQ=255)                                                                 gagaCAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAg                                                                                     >  1:446345/1‑149 (MQ=255)                                                                             gcTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTAGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCt                                                                         >  1:30053/1‑149 (MQ=255)                                                                              cTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGGGCGCCTGGCGTCTg                                                                        >  2:61624/1‑149 (MQ=255)                                                                                   gATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTGCTGGGCGGTGGTTGGCAGTAG‑CGTGATGGCGGTCTGGACGATTGGCCGTCCTAATATGCTGGTCCGCTGGGGCAGAGGCGGTTTTGGCATATGGAGCGCGTGGCGTCTGGTGaa                                                                   >  1:407055/1‑149 (MQ=255)                                                                                    aTCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTaaa                                                                  >  1:575142/1‑149 (MQ=255)                                                                                          gCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGAcc                                                            <  1:361275/149‑1 (MQ=255)                                                                                              gAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCa                                                        <  2:94372/149‑1 (MQ=255)                                                                                               aaTATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCaa                                                       >  1:6256/1‑149 (MQ=255)                                                                                                       gctAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAAc                                               >  1:213408/1‑149 (MQ=255)                                                                                                             tCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTc                                         >  1:386828/1‑149 (MQ=255)                                                                                                             tCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTc                                         <  2:6256/149‑1 (MQ=255)                                                                                                               tGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTcgc                                       >  2:30872/1‑149 (MQ=255)                                                                                                                      cTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCgg                                     <  1:179130/144‑1 (MQ=255)                                                                                                                      cTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCgg                                     >  2:179130/1‑144 (MQ=255)                                                                                                                           cgcTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCCTCCTAAGATGCTGGTCCGCTTGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCCCGGTTAACTTccc                           >  1:152386/1‑149 (MQ=255)                                                                                                                             cTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCctc                         >  1:399690/1‑149 (MQ=255)                                                                                                                               ggTTGGCGGTAG‑CGTGAGGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTg                       <  2:455951/149‑1 (MQ=255)                                                                                                                               ggTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCAGCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTg                       <  2:47266/149‑1 (MQ=255)                                                                                                                                          g‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTACCTATGGCCGGAACCAt            >  1:93240/1‑149 (MQ=255)                                                                                                                                             gTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTc          <  2:213408/149‑1 (MQ=255)                                                                                                                                                aTGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCgg       >  2:208033/1‑149 (MQ=255)                                                                                                                                                     gATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAAGGCCTTGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCCGCGCCATTCCGGCCttg  >  2:251638/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GCAACTGCTTAGCCAGATCCAGCAACAACGGCTGGATCTTTCCGCCAGTCGTCGTGAATGGCTGGAGACAACAGGCGCTTACGATCGTCGCTGGAATATGCTGCTAAGTCTGCGCTCCTGGGCGCTGGTTGGCAGTAG‑CGTGATGGCGATCTGGACGATTCGCCATCCTAATATGCTGGTCCGCTGGGCCAGACGCGGTTTTGGCGTATGGAGCGCCTGGCGTCTGGTTAAAACGACCCTCAAGCAGCAACAGCTTCGCGGTTAACTTCCCTCTGGCCGGAGCCATTCCGGCCTTA  >  NC_000913_3_pae_tpiA/3250112‑3250407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.