Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,544,022	C→A	L13F (TTG→TTT)	narU ←	nitrate/nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,544,022	0	C	A	98.9%	299.2 / NA	91	L13F (TTG→TTT)	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (49/40);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC  >  NC_000913_3_pae_tpiA/1543879‑1544161                                                                                                                                                  |                                                                                                                                              ttGAGATTAATGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         <  1:1498569/149‑2 (MQ=255)   gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaaga                                                                                                                                       <  1:1325449/149‑4 (MQ=255)    agaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaagat                                                                                                                                      <  2:2005271/149‑5 (MQ=255)     gaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaagata                                                                                                                                     <  1:732946/149‑6 (MQ=255)       ttAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑Ac                                                                                                                                   <  1:311915/149‑1 (MQ=255)                    cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTtctc                                                                                                                      <  2:289634/149‑1 (MQ=255)                    cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         <  2:1899377/130‑2 (MQ=255)                    cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑A‑aaaaa                                                                                                                                         >  1:1899377/1‑129 (MQ=255)                           tAGCATCCATACACAGAAGGTAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGc                                                                                                               <  1:8418/149‑1 (MQ=255)                           tAGCATCCAGACACAGAAGGAAAGCAGTAGACGACTAAGTGATATCCAGAGATTTCTTCGAGCAAGATGGTTTCCTTTATTGTCCCAGTAGGGCGGATTTTCTGGTTTCAAGTCG‑CG‑CAAAAAGTGA‑ACGACTATTTTTATCCGTTTGc                                                                                                               >  2:1804303/1‑149 (MQ=255)                            aGCATCCATACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  2:1422662/149‑1 (MQ=255)                            aGCATCCAAACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  1:251644/149‑1 (MQ=255)                            aGCAACCAGACTCAGAAAGTAAGAAGTAGACAACTGACTCATATGCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGCAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCa                                                                                                              <  2:2038725/149‑1 (MQ=255)                               aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCGATGAGGAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGcgttg                                                                                                           >  1:939265/1‑145 (MQ=255)                                acaaGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAAAAA‑A‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGc                                                                                                          <  2:1424984/146‑1 (MQ=255)                                   aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGTAATATGATTTCCTTTGTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTCTTTTTCTCATTTTGCAGAGCCat                                                                                                       >  1:1758291/1‑149 (MQ=255)                                   aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCAttt                                                                                                                  <  2:61399/138‑1 (MQ=255)                                   aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCAat                                                                                                       >  1:900726/1‑149 (MQ=255)                                   aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCAttt                                                                                                                  >  1:61399/1‑138 (MQ=255)                                   aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                       >  2:1689120/1‑149 (MQ=255)                                     acacAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCatat                                                                                                     >  2:201601/1‑149 (MQ=255)                                          gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                <  2:1139827/149‑1 (MQ=255)                                          gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                <  1:1333673/149‑1 (MQ=255)                                                aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  1:10618/1‑149 (MQ=255)                                                aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          <  1:201601/149‑1 (MQ=255)                                                aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:22206/1‑149 (MQ=255)                                                aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTGCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:132615/1‑149 (MQ=255)                                                aagaagTAGACAACTGACTGATATAAAGAGAGTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCGGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                          >  2:1803161/1‑149 (MQ=255)                                                 agaagTAGACAACTGACTGATATCCAGAGATTTCGTCGAGAACTTTCGGTATCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                         <  1:1526070/149‑1 (MQ=255)                                                     gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac                                                                                     >  2:161023/1‑149 (MQ=255)                                                        gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcccaca                                                                                  >  2:557252/1‑149 (MQ=255)                                                        gACAACTGACTGATATCCAGAAATTCGTTTGATCTATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTACCATATTGTTCCTCACAtcccacca                                                                                  >  2:770018/1‑142 (MQ=255)                                                           aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                               >  1:39436/1‑149 (MQ=255)                                                           aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg                                                                               <  1:41340/149‑1 (MQ=255)                                                             ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                             <  1:1839796/149‑1 (MQ=255)                                                             ctgactaaTATCCAGAGATTTCTTCCAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                             <  1:66143/149‑1 (MQ=255)                                                              tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCAAATGCACACATTGGTa                                                                            >  1:500342/1‑149 (MQ=255)                                                              tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGGGCTATATTGTTCCTCACCTGCCCACATTGGTa                                                                            >  1:688544/1‑149 (MQ=255)                                                                  tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATATACCA‑AAGGGTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                        >  1:2040828/1‑149 (MQ=255)                                                                  tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                        >  1:1457055/1‑149 (MQ=255)                                                                    atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCCCACATTGGTAATGaaa                                                                      >  2:1603872/1‑149 (MQ=255)                                                                       tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                   >  2:344991/1‑149 (MQ=255)                                                                        caaaagagTTCTCGGAGAAATATGTTTTCCTTTATTTACCCAGGATGCCGGATTTTCTGGTTAAAAAAAA‑AGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                  <  2:1758291/145‑1 (MQ=255)                                                                         cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                 >  1:1537224/1‑149 (MQ=255)                                                                          agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAACGa                                                                >  1:1145772/1‑149 (MQ=255)                                                                          agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                >  1:1782910/1‑149 (MQ=255)                                                                             gaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                             <  1:875321/149‑1 (MQ=255)                                                                               tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCATTGCCATATTGTTCCTCACATCTACACATTGCTAATGAAAAAAGAACaaaa                                                           >  2:1368087/1‑149 (MQ=255)                                                                                  cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                        >  1:883874/1‑149 (MQ=255)                                                                                   ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  2:2063686/1‑149 (MQ=255)                                                                                   ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  1:1167167/1‑149 (MQ=255)                                                                                   ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAGAGACAAAACACg                                                       >  1:571945/1‑149 (MQ=255)                                                                                   ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                       >  2:1673251/1‑149 (MQ=255)                                                                                    tcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                      <  2:559025/149‑1 (MQ=255)                                                                                         cAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa                                                 <  1:1488436/149‑1 (MQ=255)                                                                                              tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                  >  2:859892/1‑95 (MQ=255)                                                                                              tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                  <  1:859892/95‑1 (MQ=255)                                                                                                 tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAAt                                         >  1:865899/1‑149 (MQ=255)                                                                                                   tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAg                                       <  1:1976177/149‑1 (MQ=255)                                                                                                   cccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAg                                       <  1:768149/148‑1 (MQ=255)                                                                                                     cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                     >  1:197575/1‑149 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                >  1:1250452/1‑121 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                <  2:1250452/121‑1 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                    <  2:1368581/149‑1 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACAAGAGGTAAGGCGCAATATCCa                                    >  1:140526/1‑149 (MQ=255)                                                                                                      tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGGCG‑CGAA‑AAAAGAAC‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCa                                    <  1:280804/149‑1 (MQ=255)                                                                                                       ttATTTTCCCAGAAGGCCGGATTTTCCGGTTCCCAGTCGCCA‑T‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATCGTGACTCTCATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                   <  1:710653/149‑1 (MQ=255)                                                                                                         aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑TGAA‑AAAAGATA‑ACGACTATTTTTCTGATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTGATGAAAAAAAGAAAAAACACGAGGCAAGACGCAATACCCAGtt                                 >  2:944710/1‑149 (MQ=255)                                                                                                         aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                            >  2:8511/1‑90 (MQ=255)                                                                                                         aTTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                            <  1:8511/90‑1 (MQ=255)                                                                                                          ttttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGtta                                >  1:2010574/1‑149 (MQ=255)                                                                                                            ttacctgAAGGCGGGATTTTCGGTTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                              <  2:670889/143‑1 (MQ=255)                                                                                                            ttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                              >  1:1770924/1‑149 (MQ=255)                                                                                                             tCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAACCAGttatta                             >  1:949806/1‑149 (MQ=255)                                                                                                               ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAAGACGCGGTAAGGCGCAATAGCCAGTAATTAGa                           >  2:955121/1‑149 (MQ=255)                                                                                                               ccAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCAATTTGCAGTGCCATATTGTTCCTCACATGCACACAGTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGataataaa                           >  2:1579158/1‑147 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                          >  2:1232080/1‑149 (MQ=255)                                                                                                                cAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGaa                          >  1:1494611/1‑149 (MQ=255)                                                                                                                    acgCCGGAGTTTCTGGTTTCCAGTAG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                      <  2:310432/147‑1 (MQ=255)                                                                                                                    aGGCCGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                      <  2:39436/149‑1 (MQ=255)                                                                                                                       ccGGAATTTCTGGTTTCAAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGGAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGCGGTAAGGCGCATTAGCCAGCTATTAAACTCAAGGa                   >  2:2082106/1‑149 (MQ=255)                                                                                                                        cgaaTTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt                  <  1:1980888/146‑1 (MQ=255)                                                                                                                        cGGATTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt                  >  2:162676/1‑149 (MQ=255)                                                                                                                           aTTTTCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa               <  1:1705044/149‑1 (MQ=255)                                                                                                                            ttttCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTTTTTTGCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAAt              <  1:389120/149‑1 (MQ=255)                                                                                                                              ttCTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTg            <  1:661041/149‑1 (MQ=255)                                                                                                                                cTGGTTTCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg          <  1:1686021/149‑1 (MQ=255)                                                                                                                                    tttCCAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTACAGGTCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGaa      <  1:1437058/149‑1 (MQ=255)                                                                                                                                       ccAGTCG‑CGAA‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTg   >  1:324716/1‑149 (MQ=255)                                                                                                                                        cAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGc  >  1:1157101/1‑149 (MQ=255)                                                                                                                                        cAGTCG‑CG‑AAAAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGc  >  2:1851529/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                              TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCG‑CG‑C‑AAAAGATA‑ACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGC  >  NC_000913_3_pae_tpiA/1543879‑1544161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑