| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 1,544,021 | 1 | . | A | 84.2% | 45.2 / 3.6 | 19 | coding (40/1389 nt) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base A (7/9); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| * | NC_000913_3_vch_tpiA | 1,544,022 | 0 | C | A | 100.0% | 48.7 / NA | 19 | L13F (TTG→TTT) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/9); total (10/9) | |||||||||||
AACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAG > NC_000913_3_vch_tpiA/1543887‑1544171 ||| aaCGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGa < 1:347572/149‑1 (MQ=255) tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTtc > 2:284096/1‑149 (MQ=255) ggcggTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGCCTATTTTtc > 2:382748/2‑149 (MQ=255) aTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTg < 1:284096/149‑1 (MQ=255) aGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCCATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTACTGTGGGG‑A‑AGAT‑AGTTAACGACTATTTTTCTCATTTTGTAGTGCGat > 1:13129/1‑149 (MQ=255) gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc < 1:475708/149‑1 (MQ=255) gCAAGAAGTAGACAACTGACTGATATCAAGCGCTTACTTCTAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCtaa > 1:508847/1‑147 (MQ=255) aagaagTAGACAACTGACTGATATCCAGAGATTTCATCGCACAATATGTTTTCATTTATTTTCACAGAAGTCCGGCTTTTGTGGTTTCCTGTCGCG‑A‑A‑AATAGATACCGACTATTTTTCTCATGTTGAAATGACAAAATGTCCCGcaca > 2:229802/1‑149 (MQ=255) aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCAAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAA‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTg > 1:340016/1‑149 (MQ=255) tgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACGAATTTTCGCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa < 1:230975/149‑1 (MQ=255) atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaa > 1:78486/1‑149 (MQ=255) gcGAGCAATAGTGTTTCCTCTATTTTCCCAGATGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa < 2:96991/148‑1 (MQ=255) cAATATGTTTTCCTTTATTTTCCTAGAAGGGCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAAAGACTAATTTTCACATTGTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTaa < 2:213951/149‑1 (MQ=255) aaTATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca < 1:321533/111‑1 (MQ=255) aaTATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca > 2:321533/1‑111 (MQ=255) tatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc < 1:208687/149‑1 (MQ=255) tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAACGACAAAAAACGAGATAAGGCGCAATAGTCa > 2:126663/1‑149 (MQ=255) cccAGAAGGCCGGATTTTCTGGTTTTCAGTCGAGAA‑A‑AA‑AAATAACGAATAGTTTTCTCATTTTGCAGTGCCATATTGTTCCTCCCCGGTACAGTTTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAg < 1:45078/149‑1 (MQ=255) cGGATTTTCTGGTTTCCAGTCGCGAA‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCATATGCACACATTGGTAATGAAAACAAGACAAAACACGAGGTAAGGCTCAATAGCCAGTTATTAGAATTAAGGAt > 2:122397/1‑149 (MQ=255) tcgcgaaa‑aa‑aGATAACGACTATTTTTCTAATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGa > 1:153273/8‑149 (MQ=255) gcgaaa‑aa‑aGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAtt > 2:289201/6‑149 (MQ=255) a‑aa‑aGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAg > 1:126091/1‑149 (MQ=255) ||| AACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑A‑AA‑AGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAAG > NC_000913_3_vch_tpiA/1543887‑1544171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |