| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 3,815,810 | Δ1 bp | intergenic (‑42/+24) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 3,815,809 | 0 | G | . | 97.1% | 144.0 / ‑3.2 | 35 | intergenic (‑41/+25) | pyrE/rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (14/20); minor base A (0/1); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACT > NC_000913_3_vch_tpiA/3815664‑3815953 | gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAgcga < 1:379867/149‑4 (MQ=255) gtgCTTTTGTGCGTGTATTTCAGCGTAAACGCGCCAAACTTTCACACCTGCTTGCTAAGCGTAAATTCAATAAACTGGCGCTTATATGGTTTCATGCCTTTGCGCCTCATCTTATTTTTATACAGACAAAAAAAA‑GCGACTCATCAGTc < 2:270946/147‑1 (MQ=255) cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCCACCCATCTGCCGCCGTAAAAAt > 1:286205/1‑149 (MQ=255) ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTTCTTGCTAAGCGCAAATTCAATAAACTGGAGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTCAAAATc > 1:35554/1‑149 (MQ=255) ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTTTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAATAATc > 1:42570/1‑149 (MQ=255) tttCAGCGTAAACTCGCCAAACTTTAACACCTTTTTGCTAAGCGCAAATTTAATTATCTGGCGCTTATATGGTTTTATGCCTTCGCTTCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGttt < 1:234297/149‑1 (MQ=255) aGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCa < 1:90634/149‑1 (MQ=255) gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCGGTTTGCCAgcc > 2:153436/1‑148 (MQ=255) aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTGATATGATTTAATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCGTAAAAATCAGTTTGCCACcgccgc > 1:68530/1‑149 (MQ=255) aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc > 1:55636/1‑149 (MQ=255) aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc < 1:463519/149‑1 (MQ=255) aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAATGGCGCTGATATGGTTTCATGGCTTCGCTCCTCATCTTACTTTTCTACAGACATAAAAAA‑GAGACTCATCCATTGCCTTAAAATTCAGTTTGCAAGCGCCGCCTTTTGc > 2:235907/1‑149 (MQ=255) cggtAACACCTGCTTGCTAAGCGCAAATTCAAGAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc < 2:42570/146‑1 (MQ=255) ttcaaCACCTGCTTGCTAAGCACAAATACAATACACTGACGCTAACAAGGGAACAGGCCGTTTCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑CCGACACATAAGCCGCCCTCACACTCAGCTTCCCACCCCCCCCCCCTGCCTCg > 2:229477/4‑149 (MQ=255) cTGCTTGCTCAGCGCAAATGCAATAAACTGGCTCTGATTTGGTTTCATGCCTTCTCTCCTCATCTTTCTTTTCTACATACAATTAATA‑TTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg < 1:30767/149‑1 (MQ=255) cttgctAAGCGCAAATTCAATAATCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat < 1:443425/149‑1 (MQ=255) gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTACCTACAATGgcttcc > 2:8188/1‑148 (MQ=255) ctagtcgcACATTCAATTAACTGTCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACATACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga < 1:261146/144‑1 (MQ=255) ctACGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga < 1:153436/149‑1 (MQ=255) ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga < 1:187702/149‑1 (MQ=255) tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGGGCCCCTACAATGgatccgat > 2:45849/1‑149 (MQ=255) tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGGCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCaga < 1:494133/149‑1 (MQ=255) cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCTAGCGCCGCTTTCTGCCGCTCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa < 1:545265/149‑1 (MQ=255) atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt < 1:441390/149‑1 (MQ=255) ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctc < 1:51904/149‑1 (MQ=255) gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt < 2:55636/149‑1 (MQ=255) cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCTTTAAAAATTTGTTTGCCAGCGCCGTCTTCTGCGTGGTTACGATGGATTCGATTCCCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg < 1:120677/149‑1 (MQ=255) cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg < 1:397937/149‑1 (MQ=255) cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg > 2:33631/1‑149 (MQ=255) cTTCGCTCCTCATCCTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTTGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg < 1:448236/149‑1 (MQ=255) ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat > 2:358083/1‑101 (MQ=255) ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat < 1:358083/101‑1 (MQ=255) ttACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGTGAAGTCTCCTACGTCGCTACAATGGATTCGATTCCCCTCGGGCCAAAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc > 1:104088/1‑149 (MQ=255) ttttCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTTGATTTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt < 1:348801/149‑1 (MQ=255) ttttCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAAAAAGATAGGTAGCTCTTCATGGGGGAACGGCTCGCCTTCt > 2:216883/1‑149 (MQ=255) aaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt < 2:286205/149‑1 (MQ=255) | GAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACT > NC_000913_3_vch_tpiA/3815664‑3815953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |