| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_tpiA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_tpiA | 2,173,361 | 0 | C | . | 95.8% | 203.8 / 0.4 | 48 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base . (24/22); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
| * | NC_000913_3_vch_tpiA | 2,173,362 | 0 | C | . | 95.8% | 203.1 / ‑0.3 | 48 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (24/22); minor base G (1/1); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC > NC_000913_3_vch_tpiA/2173215‑2173505 || gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGCCACACAAACGGCGTACAAAATGGTGAATGGGAGA‑AAAAGCAGGCTTACCGATaccaccg > 1:607743/1‑148 (MQ=255) gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATaccaccgccgta < 1:727321/149‑7 (MQ=255) aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca < 1:870432/149‑1 (MQ=255) aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTCGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca < 1:67631/149‑1 (MQ=255) ggCGACCGCCATCGCCACTAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCAAACAAGCTGCAATCAGAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACg > 2:899179/1‑149 (MQ=255) gCGACCGGCATAGCCACGAAGAAGCCGATGGTGGCAAGAGCGACAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGCATA‑AAAATCAGGCTTGACGACACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc < 1:567201/149‑1 (MQ=255) gCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc < 2:333743/149‑1 (MQ=255) ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa < 2:310372/149‑1 (MQ=255) ccGCCACAGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa < 1:985601/149‑1 (MQ=255) cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt > 2:69862/1‑149 (MQ=255) cATCGCCACGAAGAAGCCGATGGTGGCAATATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTTGGATA‑AAAATCAGGCTTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCaa < 2:764100/149‑1 (MQ=255) tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg > 1:367599/1‑149 (MQ=255) aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCACGGCCAAt > 2:607636/1‑149 (MQ=255) aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCCCATTTCCTAGCAGCAACGCCGGATCAAGGCCAATCAg < 1:1031912/149‑1 (MQ=255) aagCCGATGGTGGCAAGAACGCCAAACGGCAGCACCTGACTACCCGGTACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCTCCGTATGTCCCAGCAGCCACGCCGGATCAAGGCCAATCAGGaa < 1:798584/149‑1 (MQ=255) gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTAATTACCCGGCACACAAACAGCAATTAAAACGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCCGCAACGCCGGATCACGGCCAGTCAGGAACt > 1:296005/1‑149 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACGCAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc > 1:942078/1‑149 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc > 1:1011218/1‑149 (MQ=255) tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg < 1:69862/149‑1 (MQ=255) aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGaa < 2:1011218/149‑1 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAGATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc > 1:758331/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc > 2:45549/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc > 1:452115/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc > 1:370560/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCAcc > 1:452116/1‑149 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAACTCCTGACCGCCGAACTTCGcc > 1:192497/1‑149 (MQ=255) gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa < 2:370560/149‑1 (MQ=255) cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGCTA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCACGCCGCACCTCCCCTGTACACAACTAc > 2:401442/1‑149 (MQ=255) cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAc > 2:531826/1‑149 (MQ=255) aTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg > 2:394694/1‑149 (MQ=255) cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATACAAGATGCTG‑ATGGCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGGCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTg < 1:607636/149‑1 (MQ=255) ggCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACAACTACTAACCTGCtt > 1:62225/1‑149 (MQ=255) cacacaAACAGCAATTAAAATGGTTAGTGGGATA‑AAAATCAGGCCTGCCGATACCA‑‑CCGCCGTATGTCCCGGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAg < 2:551942/149‑1 (MQ=255) acacaAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc < 2:367599/149‑1 (MQ=255) acaAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa > 2:916726/1‑149 (MQ=255) aaaCAGCAATTAAAATGGTGAGAGGGATA‑AAAATCAGGATTGCCGATACCA‑‑CCGCCGTATGTCAAAGAAGCAAAGCAGGATAAAGGCCAATAAGGAACTCCTGACGGCCGAACTTCGCCGGTAAACGACAAAGAGCATGATCAGCGATg > 2:188965/1‑149 (MQ=255) ttAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa > 2:331415/1‑149 (MQ=255) tAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc < 1:1647/149‑1 (MQ=255) tAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc < 2:520843/149‑1 (MQ=255) aaTGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat < 1:331415/149‑1 (MQ=255) gAGTGGGATA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga < 1:45549/149‑1 (MQ=255) aTA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt > 1:495587/1‑149 (MQ=255) tA‑AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCGGCAGCAACGCCGGATCAGGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGCGCCTGCTTAGCGATGCACGTTACACACTCCATGATggttgta > 1:270067/1‑144 (MQ=255) a‑aaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 2:648271/149‑1 (MQ=255) a‑aaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa > 2:862092/1‑149 (MQ=255) a‑aaaaTCAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 2:212215/149‑1 (MQ=255) aTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAAGCGCCCGCTTAGCGATGGGCGTTAAACCCTCCACGATGGGTTTAATCAc > 2:375643/1‑149 (MQ=255) gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc < 1:1014961/71‑1 (MQ=255) gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc > 2:1014961/1‑71 (MQ=255) gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGTCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg < 1:675044/149‑1 (MQ=255) atacccccGCCGTATTTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGACCCTGCTTCGCGATGGGCGTTAAACCACCCATGATGGGTTTAATCACCCGTGGCATTagc < 2:927525/145‑1 (MQ=255) || GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATA‑AAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC > NC_000913_3_vch_tpiA/2173215‑2173505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |