Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.45.6% inf / inf 999intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (306/221);  new base . (321/120);  total (634/365)
Fisher's exact test for biased strand distribution p-value = 2.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-01

Only 100 of 1237 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTAAAAGAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                    |                                                                                                                                  
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttt                                                                                                                                  >  1:228357/1‑128 (MQ=255)
  aataTTTAGTTTCATTAAATTATAGCAATTGGATGTAATTGTAATAAAAAAAAACTATTATCTTTTTATATATTTTTTGTATA‑TGCAAATTTTTAAAAAAA‑TAATTTTAAATTGT‑AAAAAATAGC‑‑ttt                                                                                                                                  <  2:1812682/128‑1 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑tttt                                                                                                                                 >  2:944232/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑tttt                                                                                                                                 >  2:395949/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑tttt                                                                                                                                 >  1:2969390/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑t                                                                                                                              >  1:3815889/1‑128 (MQ=255)
      tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑tt                                                                                                                             >  2:2970399/1‑128 (MQ=255)
        tattttCATTAAATTATAGCACTTTTTTTAATTAGTAACAAAATAAAAATAATATCTTTTTATATATTTTTTTTATA‑TGCAAATTTTTTAAAAAT‑TAATTTTAACTTTT‑AAAAAATAGC‑‑ttttt‑tttt                                                                                                                           <  1:3449709/125‑1 (MQ=255)
        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑tttt                                                                                                                           >  1:1148259/1‑128 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑tttttt                                                                                                                         >  1:1043048/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑ttttttt                                                                                                                        >  2:1875736/1‑128 (MQ=255)
           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑ttttt‑ttttttt                                                                                                                        >  1:2354649/1‑128 (MQ=255)
                     ttttCGCACTTTTATTTAATAGTAATTAAATAAAAATATTATCTTTTTATATTTTTTTTTTATA‑TGCAAATTTTTAAAAAAT‑TAATTTTAACTTTT‑AAAAAAAAGC‑‑ttttt‑tttttttttttttatat                                                                                                              <  1:3153638/125‑5 (MQ=255)
                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATATC‑‑TTTTT‑TTTTTTTTTTTTTtaaa                                                                                                              >  2:3837962/1‑126 (MQ=255)
                       aTCGCAGTTGTTTGAATTATTAATAAACAAAAACTAATATCTTTTTAGTAATTTTTTGTATA‑TTGAAATTTTTAAAAAAT‑TAATTTGAACTTTT‑AAAAAAAAGC‑‑TTTTT‑TTTTTTTTTTTtataatgg                                                                                                            <  1:311809/128‑5 (MQ=255)
                        ttgCACTTGTTTGAATTATTAATAAAATAAAATTATTATCTTTTTTTTTATTTTTTTTATA‑TGCAAATTTTTTAAAAAT‑TAATTTTAAATTTT‑AAAAAATTG‑‑‑GTTTT‑TTTTTTTTTTTTTTATAATgg                                                                                                          <  2:514181/126‑1 (MQ=255)
                        tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TTCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTtaataaat                                                                                                           >  1:1591967/1‑126 (MQ=255)
                         cggatttttttGAATTAGTAATAAAATAAAATTTTTATATTTTTATATATTTTTTTTATT‑TGTAAATTTTTAAAAAAT‑TAATTTTAACTTGT‑AAAAAATATC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGa                                                                                                         <  2:2172952/118‑1 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTTAACTTGT‑AAAAAATAGC‑‑ttttt‑ttttttttttttaaaattaaa                                                                                                          >  1:505778/1‑119 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCATATTAGATATTTATTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAATATATC‑‑ATTTT‑TTATTTTATTATTTaaaagaa                                                                                                          >  1:765246/1‑125 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATATC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATTTAAata                                                                                                     >  1:2060753/1‑128 (MQ=255)
                              ttttATTAATTATTTATAAAATAAAAATATTATATTTTTTTATATTTTTTTTATA‑TTTAAATTTTTAAAAAAT‑TAATTTTAAATTTT‑AAAAAATATC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAata                                                                                                     <  1:881909/125‑1 (MQ=255)
                               tGTATTAATTAATTATAAACAAAAAATATTATTTTTTTAGTTATTTTTTGTATA‑TGCAAAATTTTAAAAAAT‑TAATTTTAACTTGT‑AAAAAAAAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGGAAATA‑Taa                                                                                                 <  1:1294737/128‑1 (MQ=255)
                                 tATTAATTATTTATAAAATAAAACTATTATATTTTTTTATTTTTTTTTTATA‑TTTAAATTTTTATAAAAT‑TTATTTTAAATTTT‑AAAAAATATC‑‑TTTTTATTTTTTTTTTTTTTATAATGGAAAta‑ta                                                                                                  <  2:2851469/128‑1 (MQ=255)
                                 tATGAATTATTAATAAAATAAAAATATTATTTTTTTAGATATTTTTTGTATA‑TTCAAATTTTTAAAAAAT‑TAATTTGAACTTTT‑AAAAAATAGC‑‑‑‑‑TT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAAtt                                                                                              <  1:1409667/128‑1 (MQ=255)
                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTAGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTAATAATTAAAAAA‑Taaa                                                                                                >  1:2486160/1‑128 (MQ=255)
                                  aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATtaaaata‑aaat                                                                                                >  2:2765429/1‑127 (MQ=255)
                                   ttaaTTAGTAATAAAATAAAAATATTATCTTTTTATTTATTTTTTGTATA‑TTCAAATTTTTAAAAAAT‑TAATTTTAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAAtt                                                                                              <  1:3349412/126‑1 (MQ=255)
                                    ttatttttaattaacaaaaaataTTTTTTTTTTATATTTTTTTTTTTTA‑TTCAAATTTTTAAAAAAT‑TTATTTTAAATTTT‑AAAAAATATC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAAtt                                                                                              <  1:3911621/116‑1 (MQ=255)
                                    ttatatttataaaaataaaaataTTATTTTTTTTTTTATTTTTTTTTTA‑TTCAAATTTTTAAAAAAA‑TAATTTTAATCTTT‑TAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAAtt                                                                                              <  2:346784/117‑1 (MQ=255)
                                     aattttttaGAAAATAAAAATTTTTTCTTTTTTTATATTTTTTTTATT‑TTAAAATTTTTAAAAAAT‑TAATTTTTAATTTT‑AAAAAATA‑‑‑‑TCTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑a                                                                                          <  2:1411919/120‑1 (MQ=255)
                                     aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAAtttt                                                                                            >  1:3529887/1‑128 (MQ=255)
                                       ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTAATAATAAAAAAA‑TAAATTTT‑a                                                                                          >  2:1016795/1‑128 (MQ=255)
                                        ttttaattaaataaaaattTTTTTTTTTTTTATTTTTTTTTTTTA‑TTAAAATTTTTAAAAAAT‑TAATTTTTAATTTT‑AAAAAATTTT‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑Ag                                                                                         <  2:3084553/117‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGTTAATA‑TAAATTTT‑att                                                                                        >  1:3659624/1‑126 (MQ=255)
                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTTAAATTTA‑AAAAAAAAGA‑‑TTTTT‑TTTTTTTTTTTTT‑‑TAATTGAAATATTAAATTTT‑Agtt                                                                                       >  2:1284220/1‑126 (MQ=255)
                                            aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTAAAATTTAAATA‑AAAATTTT‑attaatt                                                                                    >  1:1827511/1‑122 (MQ=255)
                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTA‑AATTTAAAAA‑AAAAATTT‑ATTTAtttt                                                                                  >  2:3685281/1‑128 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAGT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATTTAATTA‑TATAtttttttttttttg                                                                                  >  2:1628010/1‑118 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATATCT‑TTTTT‑TTTTTTTTTTTTTTATAATGTAAA‑A‑TAAAtttttttgttttt                                                                                   >  2:2061177/1‑119 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATTAAAATT‑AAAATTTT‑attatttttt                                                                                 >  2:1131141/1‑119 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGCT‑TTTTT‑TTTTTTTTTTTTTTATAATTAAAAAA‑AAAATTTA‑ATTTAtttt                                                                                  >  1:1478013/1‑128 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTTTTttgtttta                                                                            >  1:3237350/1‑125 (MQ=255)
                                                      aataattattTTTTTTTATTTTTTTTTTTTA‑TTTAATTTTTTAAAAAAT‑TATTTTTAATTTTT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTaa                                                                         <  1:1649230/124‑1 (MQ=255)
                                                       actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTT‑T‑ATGGAAATA‑TAAATTTT‑ATGTATTTTTTTGTCTaat                                                                        >  1:3754941/1‑127 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGCT‑TTTTT‑TTTTTTTTTTTTTTATAATTTAAATA‑TAATTTTT‑ATTTATTTTTTTTTATaa                                                                         >  1:657927/1‑128 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAATTTT‑ATGTATTTTTTTTTATaat                                                                        >  1:3289185/1‑127 (MQ=255)
                                                          tttatTTTTTTTGTTATTTTTTTTTTA‑TGCAAATTTTTAAAAAAT‑TAATTTTAAATTTT‑AAAAAATATC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTa                                                                      <  2:2525662/127‑1 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGCT‑TTTTT‑TTTTTTTTTTTTTTAAAATTTAAATA‑TAAATTTT‑ATTTATTTTTTTTTATaatt                                                                       >  1:2795026/1‑126 (MQ=255)
                                                            tatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTAAAAATATAAATA‑AAAATTTA‑ATTTATTTTTTTATATaataaaa                                                                    >  2:2438891/1‑123 (MQ=255)
                                                            tatCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑‑TT‑TTTTTTTTTTTTTTATAATTGAAATA‑TAAATTTT‑AGGTATTTTTTTGTATAAGtatata                                                                  >  1:827457/1‑128 (MQ=255)
                                                               cTTTTTAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑‑‑T‑TTTTTTTTTTTTTTATAATGTAAATA‑AAAATTTT‑AGTTATTTTGTTTTATAAGTATATaaaaa                                                              >  2:146725/1‑128 (MQ=255)
                                                                tttttAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTTTTTTATAATTATATaaa                                                                >  1:2738657/1‑128 (MQ=255)
                                                                  tttAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATTTAAATA‑TAAATTTT‑ATTTATTTTTTTTTATAATTATATaaaaa                                                              >  1:2660234/1‑128 (MQ=255)
                                                                   ttATATTTTTTTTTTATT‑TTTAAATTTTTAAAAAAT‑TAATTTTAAATTTTAAAAAAATAG‑‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                            <  2:1343282/128‑1 (MQ=255)
                                                                   ttAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAATTTT‑ATTTTTTTTTTTTTATAATTATaaaaaaaa                                                             >  2:72715/1‑128 (MQ=255)
                                                                   ttAGATATTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGTAAATA‑AAAATTTT‑TGTTTTTTTTTTTTATAATTATGaaaaaaaa                                                            >  2:3889830/1‑128 (MQ=255)
                                                                      taatttttttGTATATTTAAAATTTTAAAAAAATATATTTTAATTTTAT‑AAAAAACTCT‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTaaaaaa                                                             <  1:3062579/124‑1 (MQ=255)
                                                                      gATTTTTTTTGTTTA‑TGTAAATTTTTAAAAAAT‑TTATTTTTACTTGT‑AAAAAATTG‑‑‑‑GTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                         <  2:3542044/128‑1 (MQ=255)
                                                                       ttatTTTTTTTATT‑TTTAAATTTTTAAAAAAT‑TATTTTTAAATTTT‑TAAAAAT‑‑T‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                        <  1:2453844/127‑1 (MQ=255)
                                                                        tatTTTTTTTATT‑TTAAAATTTTTAAAAATA‑TAATTTTAACTTTT‑AAAAAAATG‑‑‑‑GTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaa                                                       <  1:2014065/128‑1 (MQ=255)
                                                                         atTTTTTGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATTTAAATA‑TAAATTTT‑AGTTATTTTTTTGTATAATTATATAAAAAAATATaaa                                                      >  1:1004419/1‑128 (MQ=255)
                                                                          ttttttGTATA‑TGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAATTTT‑TTGTATTTTTTTTTATAATTATCTAATAAAAAATAAAg                                                     >  2:211544/1‑128 (MQ=255)
                                                                              tttttttatttaaaTTTTTAAAAATT‑TAATTTTAAATTTT‑AAAAAATA‑T‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                                                   <  2:2678483/117‑1 (MQ=255)
                                                                                tttttatttaaaTTTTTAAAAAAT‑TTATTTTAAATTTT‑AAAAAATA‑‑‑‑TCTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                                                <  2:31377/119‑1 (MQ=255)
                                                                                    a‑tGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGAAATTA‑TAAATTTT‑AGGTTTTTTGTTAAATAAGTATCAAAAAAAATAGAAAGTaaataaat                                            >  1:2126754/1‑126 (MQ=255)
                                                                                    a‑tGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTA‑AAT‑GAAAAA‑TAAATTTTAAGTTATTTTTTTTAAAAATTATATAAAAAAAAAAAATTAAAAAAATAtt                                          >  2:1559681/1‑128 (MQ=255)
                                                                                     tttttaaTTTTTTAAAAAT‑TTATTTTTAATTTT‑AAAAAATATA‑‑TTTTT‑TTTTTTTTTTTTTTATAATTGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                                             <  1:3730119/123‑1 (MQ=255)
                                                                                      tGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTT‑TAATGGAAAAT‑TAAATTTA‑TGTTTTTTTTTTTTAAAATTAAAAAAAAAAATAAAAAGTAAAAAatat                                           >  2:851565/1‑128 (MQ=255)
                                                                                      tGCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAATTTT‑TTGTTTTTTTTTTTGTAAGTATATAAAAAAATTTAAAGAAAAAAATAtt                                          >  2:1698290/1‑128 (MQ=255)
                                                                                       gCAAATTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGTAAATA‑TAAATTTT‑ATTTATTTTGTTTTATAATTATATAAAAAAATAAAAATTaaaaataattt                                         >  1:1506320/1‑122 (MQ=255)
                                                                                         aaaTTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑ATGTATTTTTTTTTTTAAGTATATAAAAAAATATAAAGAAAAAAatatat                                         >  1:3584810/1‑126 (MQ=255)
                                                                                         aaaTTTTTAAAAAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑CTTTT‑TTTTTTTTTTTTTTTTAATGGTAATA‑TAAATTTT‑ATGTATTTTTTTTTATTATTATATAAAAAAATATAAATTaaaaatttttt                                         >  1:1212057/1‑122 (MQ=255)
                                                                                          aaTTTTTAAATAAT‑TAATTTTTAATTTT‑AAAAAATA‑‑‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa                                      <  2:2279374/128‑1 (MQ=255)
                                                                                            ttttAAAA‑AAT‑TAATTTTAAATTTT‑AAAAAAT‑TT‑‑ATTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa                                    <  1:944232/128‑1 (MQ=255)
                                                                                                  tttttaaaaattTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTT‑TATTGGAAATA‑TAAATTTT‑AGGTTTTTTTTTGTATAATTAAATAAAAAAATAAAAAGGAAAAAATATTTTTAAAATTa                                >  2:3259922/9‑128 (MQ=255)
                                                                                                    aaat‑taattTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTATAATTTTATAAAAAAATATAAAGTAAAAAATATTTTTAAATGTAATAc                            >  1:2603504/2‑128 (MQ=255)
                                                                                                     aat‑taattTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTAAAATGGAAATA‑TAAATTTT‑AGGTATTTTTTTTTATAATTATATAAAAAAATATAAATGAAAAATTATTTTTAAAATTAATa                             >  2:3191198/1‑128 (MQ=255)
                                                                                                      at‑taattTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTATTAATGGAAAAA‑AAATTTTT‑TGGTTTTTTTTTGTCTATGTATCAAAAAATATATAAAGTAAAAAATATTTTTTAAAGTATTag                            >  1:1743217/1‑127 (MQ=255)
                                                                                                       t‑taattTGAACTTGT‑AAAAAATAGC‑‑‑‑TTT‑TTTTTTTTTTTTTTATAATTGAAATA‑TAAATTTT‑AGGTATTTTGTTGTATAAGTATATAAAAAAATATAAAGTAAAAAATATTTTTAAATTtaataataa                         >  1:2410813/1‑128 (MQ=255)
                                                                                                         taattTGAACTTGT‑AAAAAATAGCTTTTTTT‑TTTTTTTTTTTTTTATAATGAAAATA‑TAAATTTT‑AGGT‑TTTTTTTGTATAATTTTAAAAAAAAATAGAAATGAAAAAATTTTTTTaaattttaataa                           >  1:2213852/1‑119 (MQ=255)
                                                                                                         taattTGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGTAAATA‑AAATTTTT‑AGTTTTTTTTTTTTATATTTATATAAAAAAATTTAAAGTAAAAAATTTTTTTAAAATTATtaataa                         >  1:578544/1‑128 (MQ=255)
                                                                                                              tGAACTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTAAAATGTAAATT‑AAAATTTT‑AGTTTTTTTTTTTTAAAATTAAAAAAAAAAAAAAAATGAAAAAAAAATTTTTAAAATTAAAAAAAAAattt                    >  2:64484/1‑127 (MQ=255)
                                                                                                                taatttt‑AAAAAATATT‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAattat                   <  1:1356659/122‑1 (MQ=255)
                                                                                                                 aCTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATTGAAATT‑TAAATTTT‑AGTTTTTTTTTTGTCTAAGTATATAAAAAATTATAAAGTAAAAAATATTTTTAAATTTAATAATAAAAtttttt                 >  2:3025652/1‑128 (MQ=255)
                                                                                                                  ttttt‑AAAAAATTAT‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                 <  2:3345002/124‑1 (MQ=255)
                                                                                                                  cTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTT‑TATTTGAAATA‑AAAATTTT‑AGGTTTTTTTTTTTCAATTTATAAAAAAAAAAAAAAAGGAAAAAATATTTTTAAAAATAATAAAAAAATTATTTa                >  2:443098/1‑128 (MQ=255)
                                                                                                                  cTTGT‑AAAAAATAGC‑‑‑TTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTATATTTTTATAAAAAAATATAAATGAAAAAATATTTTTAAAATTAATAATAAAATTATTTa                >  1:2553643/1‑128 (MQ=255)
                                                                                                                        ttttaaaattttttttt‑ttttttttttttttATAATTTAAATA‑TAAATTTT‑AGTTATTTTTTTGTCTAAGTATCTAAAAAAATAGAAAGTAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  1:1922536/116‑1 (MQ=255)
                                                                                                                        tttaaaaattttttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt              <  2:3724745/116‑1 (MQ=255)
                                                                                                                           taataatttttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTATAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtt           <  2:2383212/119‑1 (MQ=255)
                                                                                                                           aaaTAGC‑‑TTTTT‑TTTTTTTTTTTTTTA‑AATGGAAATT‑TAAATTTT‑AGGTATTTTGTGGTATAATTATCTAAAAAAATAGAAAGGAAAAAATATTTTTAAAATTAATATAAAAATTATATAATGttaat        >  1:3397602/1‑128 (MQ=255)
                                                                                                                            aaatttttttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtta          <  2:1209090/120‑1 (MQ=255)
                                                                                                                            aaaaaatgttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtta          <  2:3424589/120‑1 (MQ=255)
                                                                                                                                tgttttttt‑ttttttttttatttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  2:3768508/124‑1 (MQ=255)
                                                                                                                                atttttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  1:417996/124‑1 (MQ=255)
                                                                                                                                aattttttt‑ttttttttttttttATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta      <  2:3624151/124‑1 (MQ=255)
                                                                                                                                 tttttttt‑ttttttttttttttATAATTGAAATA‑TAAATTTT‑ATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaattaa     <  1:288468/125‑1 (MQ=255)
                                                                                                                                 c‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑AAAATTTT‑AGTTTTTTTTTTTTGTAATTATAAAAAAAAATAAAATGTAAAAAATATTTTTAAAATAAATAAAAAAATTATTTAATTTAAATAaaaa   >  2:1963469/1‑128 (MQ=255)
                                                                                                                                    ttttt‑ttttttttttttttATAATGTAAAAA‑TAAATTTT‑TGTTATTTTTTTGTATAAATATCAAAAAAAATATAAAGTAAAAAATATTTTTAAAAATAATAAAAAAATTATTTAATTTTaaaaaaaaa  >  2:1083984/1‑127 (MQ=255)
                                                                                                                                    |                                                                                                                                  
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAAATTTTAAAAGAAT‑TAATTTGAACTTGT‑AAAAAATAGC‑‑TTTTT‑TTTTTTTTTTTTTTATAATGGAAATA‑TAAATTTT‑AGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑