Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,3030CA34.9% 2230.2 / 227.7 1309intergenic (+267/‑275)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (263/545);  new base A (342/91);  total (666/643)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1321 total aligned reads displayed.

AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGTTTGA  >  CP016816/165175‑165430
                                                                                                                                      |                                                                                                                                 
aaatatattatATTTTTTTTTTTTTTTTTTTTATTTATA‑TTTTTAAAAAA‑TTAATTTTAAATTTTTAAAAAT‑‑TT‑TTTTTTTTTTTTTTTTTTTATA‑ATTGAAAT‑ATAAATTTTAGGTATTTTGTTGTc                                                                                                                                   <  1:660199/123‑1 (MQ=255)
    ctaTTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑ATAAATTTTATTTATTTTTTTTTATaa                                                                                                                                >  2:857997/1‑128 (MQ=255)
       ttatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTTTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGTAAAT‑ATAAATTTTAGGTATTTTTTTGTATAAGTAta                                                                                                                           >  1:3668587/1‑127 (MQ=255)
        tttattttttTATTTTTTTTGTTTATGCAAA‑TTTTTAAAAAA‑TTTATTTTTACTTTTAAAAAATT‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                                                                                                            <  2:3458019/124‑1 (MQ=255)
        tatCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTAAA‑ATTTAAAT‑ATAATTTTTATGTTTTTTGTTGTATAATTAtat                                                                                                                          >  2:710397/1‑126 (MQ=255)
          tttttttttttatTTTTTTTATTTTTATA‑TTTTTAAATAATTTAATTTTAATTTTTAAAAATAT‑TT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                                                                                                           <  2:1693658/118‑1 (MQ=255)
             ttttttatatTTTTTTTATATTTAAA‑TTTTTTAAAAT‑TTTATTTTAAATTTTAAAAAATA‑TT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑aaa                                                                                                                      <  1:867427/122‑1 (MQ=255)
             ttttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑ATAAATTTTATTTATTTTTTTTTATAATTAtaaaaa                                                                                                                       >  1:231959/1‑123 (MQ=255)
             ttttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTAAA‑ATTGAAAT‑AAAAATTTTAGTTATTTTTTTGTCaaaatcgtaaaag                                                                                                                      >  1:3017639/1‑118 (MQ=255)
              tttttttatTTTTTTTTTATGTAAATTTTTAAAAAAT‑TTAATTTTAA‑TTTTAAAAAATA‑TC‑‑TTTTTTTTTTTTTTTTATATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑aaaaa                                                                                                                    <  2:2738577/122‑1 (MQ=255)
               ttAGATATTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑G‑‑ATTTTTTTTTTTTTTTTATAAA‑ATTTAAAT‑ATAAAATTTATTAATTTTTTTTTATAATTATAT‑aaaaaa                                                                                                                   >  1:719889/1‑128 (MQ=255)
                   atatTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATGTAAAT‑ATAAATTTTAGGTATTTTTTTTTCTAATTATAT‑AAAAAAATaa                                                                                                               >  1:3532185/1‑127 (MQ=255)
                     atTTTTTGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑ATAAATTTTAGTTATTTTTTTGTATAATTATAT‑AAAAAAATA‑Taaa                                                                                                           >  1:1004419/1‑128 (MQ=255)
                       tttttGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTATAAGTAACT‑AAAAAAATA‑GAAAg                                                                                                          >  2:3969593/1‑128 (MQ=255)
                       tttttGTATATGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGTAAAT‑ATAAATTTTAGGTATTTTTTTGTATATGTATAT‑AAAAAAATA‑GAAAgt                                                                                                         >  1:411629/1‑127 (MQ=255)
                         tttttttatttaaa‑TTTTTAAAAAT‑TTAATTTTAAATTTTAAAAAATA‑‑T‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGa                                                                                                        <  2:2678483/117‑1 (MQ=255)
                             ttaatgtaaatatttAAAAAAT‑TAAATTTAAATTTTTAAAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTTTA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGaaa                                                                                                      <  2:3079804/116‑1 (MQ=255)
                             tataTGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑AAAAATTTTATTTATTTTTTTTTATAATTTAAT‑AAAAAAATA‑TAAATTaaaaa                                                                                                    >  2:464833/1‑128 (MQ=255)
                                attcAAA‑TTTTTTAAAAA‑TTAATTTTAAATTTTAAAAAATA‑TT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAat                                                                                                  <  2:3747340/125‑1 (MQ=255)
                                 tGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATTGAAAT‑ATAAATTTTAGTTTTTTTGTTTTATAATTTAAA‑AAAAAAATA‑TAAATAAAAAAata                                                                                                 >  2:3879616/1‑128 (MQ=255)
                                 tGCAAA‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑‑‑TTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATTAATTTTAGGTATTTTGTTTTATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGt                                                                                             >  1:2681060/1‑128 (MQ=255)
                                  ttaaa‑TTTTTTAAAAA‑TTAATTTTTATTTTTATAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAatat                                                                                                <  1:3757204/126‑1 (MQ=255)
                                    aaa‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑ATAAATTTTATATTTTTTTTTTTATAAATATAT‑AAAAAAATA‑TAATTAAAAAAATATtttt                                                                                            >  1:38935/1‑128 (MQ=255)
                                    aaa‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTAAA‑ATTAAAAT‑ATAAATTTTATTTATTTTTATGTATAATAATATAAAAAAAATA‑aaaaaaaaaaaaatattt                                                                                             >  2:320040/1‑122 (MQ=255)
                                      a‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTAAAA‑ATGTAAAT‑ATAAATTTTAGGTATTTTTTGGTAAATGTAAAA‑AAAAAAATA‑GAAAGAAAAAAAATTTGTTa                                                                                           >  2:3284552/1‑128 (MQ=255)
                                      a‑TTTTTAAAAAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGTAAAT‑ATAAATTTTTGTTTTTTTTTTTTATAATTATAT‑AAAAAAATA‑AAAAGGAAAAATTTTTTTTaa                                                                                          >  2:1111022/1‑128 (MQ=255)
                                           tttaaaaa‑ttaattTTAATTTTTTAAAAATT‑G‑‑‑TTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGt                                                                                      <  1:1265673/122‑1 (MQ=255)
                                            ttaaaaa‑tttattTTAAATTTTTAAAAATA‑TC‑‑TTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGt                                                                                      <  1:1398174/123‑1 (MQ=255)
                                             taataa‑tttattTTATATTTTAAAAAATA‑G‑‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTa                                                                                     <  1:2022328/124‑1 (MQ=255)
                                             aaaaaa‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTTTATATTGTAATAAAAAATTTTATGTTTTTTGTTTTATATTTATTT‑AAAAAAATA‑AAAAATATAAAATTTTTTTaaaa                                                                                        >  1:519781/1‑128 (MQ=255)
                                              aaaaa‑ttaattTGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTATAAA‑ATTAAAAA‑AAAAATTTTAATTAATTTTTTTTATAAAAAAAT‑AAAAAAAAA‑TAAATTAAAAAATATTTATaaataaat                                                                                    >  2:1494657/4‑123 (MQ=255)
                                               aaaa‑tttattTTTAATTTTAAAAAATATTT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTa                                                                                     <  2:367671/126‑1 (MQ=255)
                                                aaa‑ttaattTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑TAAAGGAAAAAATATTTTTAAATGTAATAc                                                                                 >  1:3152177/2‑128 (MQ=255)
                                                  a‑ttaattTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTT‑‑‑TA‑ATTTAAAT‑TAAAATTTTATTTTTTTTTTTTTATAATTTAAT‑AAAAAAATA‑TAAATTAAAAAATTTTTTTaaaatatataataa                                                                              >  1:1210819/1‑118 (MQ=255)
                                                  a‑ttaatgTGAACTTGTAAAAAATA‑GCTTTTTTTTTTTTTTTTTTTTTTA‑ATGTAAAT‑ATAATTTTTTTGTTTTTTTTTGTATAAGTTAAT‑AAAAAATTA‑AAAAGTAAAAAATTTTTTTAAATGTaaaa                                                                                  >  1:2216210/1‑126 (MQ=255)
                                                    ttaattTGAACTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTATAAGTATCT‑AAAAAAATA‑TAAAGGAAAAAATATTTTTaaaatttgatagga                                                                              >  1:2149480/1‑118 (MQ=255)
                                                          ttaaCTTTTAAAAAAT‑‑TT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTATGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAatta                                                                         <  1:258917/126‑1 (MQ=255)
                                                          tGAACTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTAAA‑ATGTAAAT‑TAAAATTTTAGTTTTTTTTTTTTAAAATTAAAA‑AAAAAAAAA‑AAATGAAAAAAAAATTTTTAAAATTAAAAAAAAAattt                                                                         >  2:64484/1‑127 (MQ=255)
                                                             aCTTGTAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTTTTTTGTTGTCTAAGTATAT‑AAAAAAATA‑GAAAGGAAAAATTATTTTTAAAAGTAATACTAAAATTAttt                                                                      >  2:3628230/1‑128 (MQ=255)
                                                             aCTTGTAAAAAATA‑GC‑‑‑TTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTTTTGTATAAGTATAT‑AAAAAAATA‑AAAAGGAAAAAATATTGTTAAAATTAATACTAAAATTATTTa                                                                     >  2:1379252/1‑128 (MQ=255)
                                                              tttttAAAAAATT‑AT‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTAttt                                                                      <  2:3345002/124‑1 (MQ=255)
                                                                  tAAAAAATA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑AAAAATTTTAGGTATTTTGTTGTATAATTATTT‑AAAAAAATA‑GAAAGTAAAAAATATTGTTAAATGTAATAATAAAATTATTTAATGt                                                                 >  1:580357/1‑128 (MQ=255)
                                                                    ttaataatatttttttttttttttttttttATA‑ATTGAAAT‑ATAAATTTTAGATATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATg                                                                  <  1:1454663/117‑1 (MQ=255)
                                                                     ttaaaaaatttttttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTATGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGt                                                                 <  2:1218966/118‑1 (MQ=255)
                                                                      aaaTA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATGTAAAT‑AAAAATTTTTGTTTTTTTTTTTTGTAATTATAT‑AAAAAAATA‑TAAATTAAAAAATATTTTTAAAATTAATAATAAAATTTTTAAATTttaa                                                              >  1:1501318/1‑128 (MQ=255)
                                                                       aaTA‑GC‑‑TTTTTTTTTTTTTTTTTTATA‑ATTTAAAT‑ATAATTTTTAGTTTTTTTTTTGTAAATTTATAA‑AAAAAAAAA‑TAAATAAAAAAATATTTTAAAAAATAAAAAAAAAATTTTTTTAGTttaatt                                                            >  2:814960/1‑128 (MQ=255)
                                                                          aatattttttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatt                                                            <  2:1157142/123‑1 (MQ=255)
                                                                           atagtttttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta                                                           <  2:3411733/124‑1 (MQ=255)
                                                                           aatttttttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta                                                           <  1:2770715/124‑1 (MQ=255)
                                                                            gC‑TTTTTTTTTTTTTTTTTTTATA‑TTTAAAAA‑AAAAATTTT‑‑TTTTTTTTTTTTTAAAATATTT‑AAAAAAAAATAAAAATAAAAAAATTTTTTAAAAATAATAATAAAAATATTTaaatttatatttaa                                                        >  2:1599397/1‑116 (MQ=255)
                                                                               tctttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTATGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAc                                                       <  1:3430898/126‑1 (MQ=255)
                                                                                 ttttttttttttttttttTA‑ATGGAAAT‑ATAAATTTTAGGTTTTTTGTTTTGTAAGTTTAT‑AAAAAAATA‑TAAAGAAAAAATTATTTTTAAAAGTAATAATAAAATTTTTTAATTTTAATTTAAAAtt                                                     >  1:945648/1‑128 (MQ=255)
                                                                                  ttttttttttttttttATA‑ATGGAAAT‑ATAAATTTTTGGTATTTTTTTGTATAATTTTAA‑AAAAAAATA‑AAAAGTAAAAAATTTTTTTTAAATTAAAAATAAATTTATTTAATTTTAATTAAAAATta                                                    >  1:1156129/1‑128 (MQ=255)
                                                                                   tttttttttttttttATA‑ATGTAAAT‑ATAAATTTTATTTATTTTGTTTATTAATTATAT‑AAAAAATTT‑AAAATAAAAAAATATTTTTAAAATTAATAATAAATTTATTTAATTTTATTTAAAAATtaa                                                   >  1:383259/1‑128 (MQ=255)
                                                                                     tttttttttttttATA‑ATGGAAAT‑ATAAATTTTTTGTTTTTTGTTTTATAATTATAT‑AAAAAAATA‑AAAAGTAAAAAATATTTTTAAAATAAATAATAAAATTATTTAATTTTAATTAAAACTaaaat                                                 >  2:315578/1‑128 (MQ=255)
                                                                                       tttttttttttATA‑ATGGAAAT‑ATAAATTTTTGGTATTTTTTTTTATAAGTATAT‑AAAAAAATA‑GAAAGGAAAAAATTTTGTTAAAATTAATAATAAAATTTTTTAATTTTAATTAAAAATtaaataa                                               >  1:48255/1‑128 (MQ=255)
                                                                                        ttttatttttatA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTtaaataaa                                              <  2:3014117/128‑1 (MQ=255)
                                                                                         ttttttttttTA‑ATTGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATaaaa                                             <  2:1780580/128‑1 (MQ=255)
                                                                                            ttttttATA‑ATTGAAAT‑ATAAATTTTAGGTATTTTGTTGTATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACAt                                          <  1:1551840/128‑1 (MQ=255)
                                                                                                   ta‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATta                                   <  1:3083973/128‑1 (MQ=255)
                                                                                                      aTGTAAAT‑ATAAATTTTAGTTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATtaat                                 <  1:245185/128‑1 (MQ=255)
                                                                                                      aTGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATtaat                                 <  2:1007126/128‑1 (MQ=255)
                                                                                                       tGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATtaata                                <  2:2493873/128‑1 (MQ=255)
                                                                                                        ggAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATAT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATtaataa                               <  2:2437606/128‑1 (MQ=255)
                                                                                                         gAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaa                              <  2:918388/128‑1 (MQ=255)
                                                                                                         gAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaa                              <  1:3270595/128‑1 (MQ=255)
                                                                                                           aaT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAATGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaaaa                            <  2:1886973/128‑1 (MQ=255)
                                                                                                           aaT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTATTaaaaa                            >  1:1688598/1‑128 (MQ=255)
                                                                                                           aaT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaaaa                            >  2:382165/1‑128 (MQ=255)
                                                                                                           aaT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaaaa                            >  1:177664/1‑128 (MQ=255)
                                                                                                           aaT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATaaaaa                            >  1:2858425/1‑128 (MQ=255)
                                                                                                             t‑ataAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATa                          >  2:225215/1‑128 (MQ=255)
                                                                                                               ataAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAg                         >  1:3724397/1‑128 (MQ=255)
                                                                                                                taAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGAAATACTAAAATTATTAAATGTTAATTAAAACTTAAATAAAACATATAATAAAAAAAAATatt                        >  2:560959/1‑126 (MQ=255)
                                                                                                                  aaTTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGttt                      >  2:2183554/1‑128 (MQ=255)
                                                                                                                   aTTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTtta                     <  2:7520/128‑1 (MQ=255)
                                                                                                                    ttttAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAATTAGTttaa                    <  2:1068720/128‑2 (MQ=255)
                                                                                                                     tttatttATTTTGTTGTATAAGTATAT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatt                   <  1:2391419/122‑1 (MQ=255)
                                                                                                                     tttAGGTATTTTGTTTTCTAAGTATCT‑AAAAAAATA‑GAAATGAAAAAATATTGTTAAAAGTAATAATAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatt                   <  1:1842298/128‑1 (MQ=255)
                                                                                                                     tttAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAACCATAAAATTAATAAAAATAGTttatt                   >  2:2898814/1‑128 (MQ=255)
                                                                                                                     tttAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatt                   <  2:2116474/128‑1 (MQ=255)
                                                                                                                     tttAGGTATTTTGTTGTATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatt                   <  2:1300367/128‑1 (MQ=255)
                                                                                                                     tttAGGTATTTTGTTGTATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatt                   <  2:3556603/128‑1 (MQ=255)
                                                                                                                      ttAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAATGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatta                  <  1:3593156/128‑1 (MQ=255)
                                                                                                                      ttAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAATAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTttatta                  >  2:2045703/1‑128 (MQ=255)
                                                                                                                       tAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTaa                 >  1:1608861/1‑128 (MQ=255)
                                                                                                                        atgTATTTTGTTTTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTaaa                <  2:2597180/126‑1 (MQ=255)
                                                                                                                            aTTTTTTTGTATAAGTATAT‑AAAAAAATA‑GATAGTAAAAAATATTGTTAAAATTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTATACTaa            <  2:2323002/128‑1 (MQ=255)
                                                                                                                            aTTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAATGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTaa            <  1:813226/128‑1 (MQ=255)
                                                                                                                             ttttGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAATAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAAt           >  1:2075572/1‑128 (MQ=255)
                                                                                                                             ttttGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAAt           <  1:1334177/128‑1 (MQ=255)
                                                                                                                             ttttGTTGTATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAAt           <  1:3197313/128‑1 (MQ=255)
                                                                                                                             ttttGTTGTAAAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAAt           <  2:1420507/128‑1 (MQ=255)
                                                                                                                              tttGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAAtt          >  1:2277033/1‑128 (MQ=255)
                                                                                                                                 gttttCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAATCTTAATTAAAACATAAAATTAATATAAATAGTTTATTAAAATAAtttag       >  2:2799206/1‑126 (MQ=255)
                                                                                                                                  ttgtCTAAGTATAT‑AAAAAAATA‑GAAAGGAAATAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGt      <  1:1083984/128‑1 (MQ=255)
                                                                                                                                  ttgtATAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTTTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGt      <  1:2951490/128‑1 (MQ=255)
                                                                                                                                    gtCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGttt    <  1:1931579/128‑1 (MQ=255)
                                                                                                                                     tCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGTTTg   <  1:2514412/128‑1 (MQ=255)
                                                                                                                                     tCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGTTTg   <  1:3353802/128‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAA‑TTTTAAAAGAA‑TTAATTTGAACTTGTAAAAAATA‑GC‑TTTTTTTTTTTTTTTTTTTATA‑ATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCTAAGTATCT‑AAAAAAATA‑GAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTTAAATAAAACATAAAATTAATAAAAATAGTTTATTAAACTAATTTCGTTTGA  >  CP016816/165175‑165430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑