BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,253	0	C	A	42.8%	1190.1 / inf	1594	intergenic (‑536/+307)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base C (303/571); new base A (547/107); total (862/732)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1621 total aligned reads displayed.

AAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACTTTTTTGTTTTTA  >  CP016816/420128‑420380

   actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTA‑‑‑aaaaaat                                                                                                                               >  2:580385/1‑127 (MQ=255)

   actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTA‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑Aaaaaaa                                                                                                                               >  2:1860877/1‑128 (MQ=255)

    caaaaagtaattttttAAAAAATTTTTAATTTTAAAAAATTAATTTATTTTAAAA‑ATTTTAAATTATTTTAAAAT‑AATTA‑AAAATTAATAAA‑TTTTATTCCC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACaaaaa                                                                                                                               <  2:3861563/118‑1 (MQ=255)

     ttattttatttttttTAAAAATTTTTTATTTTTATAAATTTATTTTTTTTTAAATTTTTTAAATTTTTTTTAAAT‑‑ATTATTATTTTAATAAA‑TTTTTTTTCG‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAa‑t                                                                                                                             <  2:175768/119‑1 (MQ=255)

     tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAa‑ta                                                                                                                            >  1:1166593/1‑128 (MQ=255)

     tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTA‑‑‑ACAAAAa‑ta                                                                                                                            >  1:4117814/1‑128 (MQ=255)

     taattttaattttttAAAAATTTTTTAATTTTAAAAAATTAATTATTTTTAAAA‑TTTTTAAATTTTTTTAAAAT‑AATTA‑TAAATTAATAAA‑TTTTATTCTCTT‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAa‑t                                                                                                                             <  2:4081661/119‑1 (MQ=255)

      actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAa‑ttt                                                                                                                           >  1:2532027/1‑126 (MQ=255)

      aatataatttttttAAAATATTTTTAATTTTTATTAATTAATTTTTTTTAAAA‑TTTTTAAATTATTTTTAAAT‑CTTTA‑TTTATTTATAAA‑TTTTTTTCTC‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAa‑tat                                                                                                                           <  1:3005001/120‑1 (MQ=255)

        tttaaatttttaAAAAATTTTTTATTTTTAATAAATTAATT‑TTTTTTAAA‑TTTTTTAAATATTTTTAAATAAATTT‑TTTTTTATAAAA‑TTTTATGATC‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATaa                                                                                                                         <  1:3992538/122‑1 (MQ=255)

        ataaatttttttAAAAATTTTTTATTTTTAATAAATTAATTATTTTTAAAA‑TTTTTAAATTATTTTAAAAA‑AATTT‑TATTTTAAAAAA‑TTTTATGTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATaa                                                                                                                         <  1:2959367/122‑1 (MQ=255)

          gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑AATAatt                                                                                                                       >  2:2459235/1‑128 (MQ=255)

            aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAattt                                                                                                                      >  2:1678974/1‑128 (MQ=255)

            aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAatta                                                                                                                      >  2:3556733/1‑127 (MQ=255)

            aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTA‑‑‑ACAAAAA‑TATAattta                                                                                                                     >  2:2292823/1‑128 (MQ=255)

            aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAattttt                                                                                                                    >  1:3678170/1‑126 (MQ=255)

             cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TTTAatttat                                                                                                                    >  1:1606109/1‑128 (MQ=255)

             cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAatatat                                                                                                                    >  1:2248549/1‑128 (MQ=255)

             attttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAA‑TTTTTAAATTATTTTAAAAT‑AATTA‑TATATTAATAAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAatttat                                                                                                                    <  2:3241251/127‑1 (MQ=255)

              ttttttAAAAAATTTTTAATTTTTATTAATTATTTATTTTTAAAA‑TTTTTAAATTATTTTTAAAT‑TATTT‑TATATTTATAAA‑TTTTTTTCTT‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAatttat                                                                                                                    <  1:4293664/128‑1 (MQ=255)

              ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTCTT‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAatttat                                                                                                                    >  2:758525/1‑128 (MQ=255)

                ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGATC‑G‑‑‑TTTTTTTTTTTTTTTTTTA‑AAAAAAA‑TAAAatttatt                                                                                                                   >  2:869432/1‑128 (MQ=255)

                  ttAACAAATTTTTAATTTTAATAAATTACTATTTTTGCAAA‑TTGTTAAAATATTTACAAAT‑CATTA‑TCTATTAATAAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGAt                                                                                                               <  1:453186/128‑1 (MQ=255)

                  ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TAAAATTTAATTGa                                                                                                                >  2:4391897/1‑128 (MQ=255)

                      aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTaaa                                                                                                           >  2:24183/1‑128 (MQ=255)

                      aaaaTTTTTAATTTTAATAAATTTATTATTTTTTAAA‑TTTTTAAATTATTTTTAAAT‑TATTA‑TATATTATTAAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTaaa                                                                                                           <  1:4186558/128‑1 (MQ=255)

                       aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTA‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTATAc                                                                                                          >  1:502557/1‑128 (MQ=255)

                             ttaattttaatAACTTAATTATTTTTAAAA‑TTTTTAAATTATTTTAAAAT‑AATTA‑TATATTAATAAA‑TTTTATTATC‑‑‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATAtt                                                                                                   <  2:2641518/120‑1 (MQ=255)

                               aatttaaatAA‑TTAATTATATTTAAAA‑TATTTAAAATATTTTTAAAT‑TATTT‑TATTTTATTTTA‑TTTTTATTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTa                                                                                                 <  1:1903444/128‑1 (MQ=255)

                                  tttaatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑AGAAAAA‑TATAATTTATTTGATTAAACTTATTTTAATGt                                                                                              >  1:561513/4‑128 (MQ=255)

                                   ttaatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTAAACTTATATTTATGt                                                                                              >  2:2744038/3‑128 (MQ=255)

                                   ttaatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATAAA‑TTTTATGATC‑GTT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TAAAATTTATTTTATTAAAAGTATAttttt                                                                                                >  2:3136735/3‑126 (MQ=255)

                                    taattaatttatttttTTTTAAT‑TTTTTTATTTATTTTAAAAT‑AATTA‑TATATTAATATA‑TTTTTT‑‑TT‑T‑‑‑TGTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTta                                                                                           <  1:638073/118‑1 (MQ=255)

                                    taatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GTT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTTTTTGATTAAActtttttttttt                                                                                               >  2:1525246/2‑117 (MQ=255)

                                    taatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTTATTAAACGTATATTTATGtt                                                                                             >  1:1271422/2‑128 (MQ=255)

                                    taatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTTATTAAAATTATATTTAtttt                                                                                             >  2:4333484/2‑128 (MQ=255)

                                     aatatatttattattTTTAAAA‑TTTTTAAATTTTTTTTAAAT‑TATTA‑TATTTTTATAAA‑TTTTTTTTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGttt                                                                                            <  1:2049503/119‑1 (MQ=255)

                                     aatAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAT‑TATATTTTATTTAATTAAACGT‑TATttttttttt                                                                                           >  1:3528665/1‑127 (MQ=255)

                                       tatttttttttttttttaat‑ttttttaatTTTTTTATAAT‑AATTT‑TTTATTTTTTAA‑TTTTTTTTTCTT‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTta                                                                                           <  1:953530/117‑1 (MQ=255)

                                       ataattaaTTATTTTTAAAA‑ATTTTAAATTTTTTTCAAAT‑AATTT‑TATATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTtaa                                                                                          <  1:3781202/122‑1 (MQ=255)

                                        aaCTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑‑TTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATTTTtaat‑aa                                                                                      >  1:3846718/1‑128 (MQ=255)

                                            taaTTTTTTTTTAAA‑TTTTTTAATTTTTTTTAAAT‑AATTA‑TATATTAATAAA‑TTTTTTTTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑aaaa                                                                                    <  2:2123510/127‑1 (MQ=255)

                                               ttattTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATg                                                                                 >  1:3616892/1‑128 (MQ=255)

                                                  ttttttaat‑tttttaaTTTTTTTTAAAAT‑‑ATTT‑TTTTTTAATTAA‑TTTTTTTCTT‑GTTTTTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATgg                                                                                <  1:4411130/128‑1 (MQ=255)

                                                  tttttCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTAAACTTATATTTATTTTTAAT‑AAAAATTGaa                                                                              >  2:667315/1‑128 (MQ=255)

                                                    tttCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATTATATTTTTTTTTATTAAA‑ATATTTTTTTTTTTTAT‑TAAAATTGaaaa                                                                            >  1:1693159/1‑128 (MQ=255)

                                                        aaa‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑AATAATTTATTTTATTAAAAGTATATTTAATTTTAAT‑AAAAATGTAaaagaaag                                                                       >  1:2432524/1‑128 (MQ=255)

                                                            ttGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑AATAATTTATTTTATTAAAATTATTTTTTTTTTTAAT‑AAAAATGaaaataaattt                                                                      >  2:3773577/1‑121 (MQ=255)

                                                             ttttattttattTTTATAT‑TATTA‑TTTTTTAATAAA‑TTTTTTTCTT‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGa                                                                    <  2:2905353/121‑1 (MQ=255)

                                                                tAAATTTTTTTTAAAT‑AATTA‑TCTATTAATTAA‑TTTTATTCT‑‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTg                                                                <  1:4473087/128‑1 (MQ=255)

                                                                 aaaTTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTTTTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGAAAAATAAATTTAATTGt                                                               >  1:3718515/1‑128 (MQ=255)

                                                                   tttattaTAAAAT‑ATTTA‑TCTATTAATTAT‑TTTTAT‑TTT‑A‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAATA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCa                                                             <  2:2354052/127‑1 (MQ=255)

                                                                      tttttAAAAT‑TATTA‑TTTTTTAATAAA‑TTTTAT‑‑TA‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCataat                                                         <  2:3705301/127‑1 (MQ=255)

                                                                      attTTCAATT‑AATTT‑TATTTTTATAAT‑TTTTTTGGTA‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAATGTTAATTGTCata                                                           <  1:3195757/128‑1 (MQ=255)

                                                                       ttttCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTTTTTAATTAAAATTAATTTTATTTTTTAT‑AAAAATGTAAAAAAAATTTAATTTTAata                                                           >  2:4248943/1‑128 (MQ=255)

                                                                       ttttCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TAAAAATTATTTTATTAAAAATATATTTATTTTTAAA‑ATAAATCTAAAATAAATTTAAttataaaaa                                                          >  2:1957259/1‑121 (MQ=255)

                                                                             aat‑aatTA‑TTTATTAATAAATTTTTTTTTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAAtaatt                                                     <  2:527690/128‑1 (MQ=255)

                                                                             aaT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTTTAATAAtatttt                                                    >  2:4003785/1‑128 (MQ=255)

                                                                             aaT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTGATTAAACGTATATTTATTTTTAAT‑AAAAATTAAAAATAAATTTAATTGTCATAAtaattt                                                    >  2:2216143/1‑128 (MQ=255)

                                                                                 cATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATATATTTAATTAAACGAATATTTATTTTTAAT‑AAAAATTGAAAATAAATATAATTTTCATAAaaatttaa‑tt                                               >  2:3352640/1‑128 (MQ=255)

                                                                                   tta‑tttatTAATAAA‑TTTTTTTTTT‑T‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATttaa‑tta                                              <  2:4154445/128‑1 (MQ=255)

                                                                                   ttA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTATTTTTATTAAACTTTTTTTTATTTTTAAT‑AAAATTGTAAAAAAATTTTATTTTAAATAAtaatttaa‑taa                                              >  2:94033/1‑126 (MQ=255)

                                                                                     a‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATTTTTAAT‑AAAAATTGAAAATAAATTGAATTGTAATAATAATTTAA‑TTaaa                                            >  2:103220/1‑128 (MQ=255)

                                                                                         tATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTTA‑‑AAAAAAA‑TATAATTATTTTTTTTAAAATTATATTTTTTTTTAAT‑AAAATTGTAAATTAAATTTATTTTAAATATAATTTTAA‑TTAAatt                                          >  1:3097596/1‑127 (MQ=255)

                                                                                         tATTAATCAA‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTTATTAAACGTATATTTATGTTTAAT‑AAAAATGTAAAAGAAATTTATTTGTAATAATAATTTAA‑TTAAatata                                        >  2:1282478/1‑128 (MQ=255)

                                                                                            tAATCAA‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TAAAATTTTTTTGATTAAACTTATATTTATTTTTAAT‑AAAAATTGAAAATAAATTAAATTGTCATAATAATTTAA‑TTAAatatat                                       >  2:4204615/1‑128 (MQ=255)

                                                                                             aaTCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTTTTAAAAAAAA‑AATAATTTATTTAATTAAAATTATATTT‑TTTTTAATAAAAAATTAAAAATAAAGTAAATTTAAATAAAAATTAAA‑TTTAatata                                        >  2:3058689/1‑128 (MQ=255)

                                                                                             aaTCAA‑TTTTATGCTC‑G‑‑‑‑‑‑TTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTTATTAAACGTATATTTATTTTTAAT‑AAAAATTAAAAATAAATTAAATTTTAATAAAAATTTAA‑TTAAATATATAaaaa                                  >  2:1624556/1‑128 (MQ=255)

                                                                                               taaa‑TTTTTTGCTC‑‑‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACaa                                  <  2:3954557/126‑1 (MQ=255)

                                                                                                  a‑TTTTATGCTC‑GT‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTAAAATTATATTTTTTTTTAAT‑AAAAATTTAAAATAAATTTAATTTTCATAATAATTTAT‑TTAAATATATTAAAAt                                 >  2:1196000/1‑128 (MQ=255)

                                                                                                  a‑TTTTATGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATTTTTAAT‑AAAAATGTAAAATAAATTGAATTGTAATAATAATTTAA‑TTAAATATATAACAAttt                               >  2:2037461/1‑126 (MQ=255)

                                                                                                   attttttttttag‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAAt                                 <  1:2664268/116‑1 (MQ=255)

                                                                                                     tttATGCTC‑G‑‑‑‑‑TTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTAAACGTATATTTATTTTTAAT‑AAAAATGAAAAATAAATTTAATTGTAATAATAATTTAA‑TTAAATATATAACAATTTAAg                            >  1:3918190/1‑128 (MQ=255)

                                                                                                         ttttttttttttttttttttttttttt‑‑‑tCAATAT‑TATAATTTATTTGTTTAATCGTATTTTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTa                              <  2:485758/118‑1 (MQ=255)

                                                                                                         tGCTC‑G‑‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTTATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAATAAAGTTATTTGTAATAAAAATTTAA‑TTAAAAATATAAAAATTTAAtttt                         >  1:1077823/1‑128 (MQ=255)

                                                                                                           cTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAAAGTATTTTTATTTTTAAT‑AAAAATGTAAAAGAAAGTTAATTGTCATAATAATTTAA‑TTAAATATATAAAAATTTAAGtttt                        >  2:4589125/1‑128 (MQ=255)

                                                                                                            tttg‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTTTTTGATTATACGTATATTTATGTTTAAT‑AAAAATGTAAAAGAAAGTGAATTGTAATAATAATTTAA‑TTAAATATATAACAATGTAAGtttt                        <  1:3475624/125‑1 (MQ=255)

                                                                                                             c‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑A‑AAAAA‑AATAATTTTTTTTTTTTAAAGTATATTTTTTTTTAAA‑AAAAATTGAAAAAAAAATTAAATTTAATTAAAATTTAATTTAAATATTAAAAAATTTAA‑TTTTaaa                     >  1:460486/1‑128 (MQ=255)

                                                                                                              tttttttttttttttttttttt‑‑‑AAAAAAA‑TATTATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGttt                         <  2:3665279/123‑1 (MQ=255)

                                                                                                               ttttttttttttttttttttt‑‑‑AAAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGtttt                        <  1:2348968/124‑1 (MQ=255)

                                                                                                               g‑‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAA‑TATAATTTATTTGATTAAACGTTTTTTTATTTTTAAT‑AAAAATGGAAAAGAAATTGAATTTTAAAAATAATTTAA‑TTAAATATATAACAATTAAATTTTTaaa                     >  2:2565502/1‑128 (MQ=255)

                                                                                                                tttttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTTAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTa                       <  2:1364072/125‑1 (MQ=255)

                                                                                                                tttttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTa                       <  1:1415436/125‑1 (MQ=255)

                                                                                                                tttttttttttttttttttt‑‑‑AAAATAA‑TATAATTTATTTGATTAAAAGTATATTTATTTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTa                       <  2:2957746/125‑1 (MQ=255)

                                                                                                                tatttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTa                       <  1:822882/125‑1 (MQ=255)

                                                                                                                cgtttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTTATTAAACTTATATTTATTTTTAAT‑AAAAATGAAAAATAAATTGAATTTTAATAATAATTTAA‑TTAAATATTTAAAAATTTAATTTTTa                       >  2:2809311/4‑128 (MQ=255)

                                                                                                                agtttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTa                       <  1:4356851/125‑1 (MQ=255)

                                                                                                                   ttttttttttttttttt‑‑‑ACAATAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATTGAAAAGAAAGTGAATTGTAATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTaaaa                    <  2:4466348/128‑1 (MQ=255)

                                                                                                                   ttttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATTTTTATGTTTATT‑AAAAATGGAAAATAAAGTGAATTGACATATTAATTTAA‑TTAAATATATAAAAATTAAATTTTTaaaa                    >  2:1461577/1‑128 (MQ=255)

                                                                                                                    tttttttttttttttt‑‑‑ATAAAAA‑TATAATTTATTTGATTAAAATTATATTTATTTTTAAT‑AAAAATGTAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAac                   <  1:2837056/128‑1 (MQ=255)

                                                                                                                     ttttttttttttttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAact                  <  2:3614362/128‑1 (MQ=255)

                                                                                                                      tttttttttttttt‑‑‑AAAAAAA‑TATAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAactt                 <  2:453003/128‑1 (MQ=255)

                                                                                                                        tttttttttttt‑‑‑ACAAAAA‑TTTAATTTATTTGATTAAAAGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAacttac               <  2:1122326/128‑1 (MQ=255)

                                                                                                                        tttttttttttt‑‑‑A‑AAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGATTTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAacttact              <  1:100117/128‑1 (MQ=255)

                                                                                                                         ttttttttttt‑‑‑ATATTAA‑TTTAATTTTTTTGATTAATAGTATATTTATTTTTAAT‑AAAAATTGAAAAGAAAGTTAATTGTAATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAacttact              <  1:2124262/128‑1 (MQ=255)

                                                                                                                           ttttttttt‑‑‑ATATTTA‑TATATTTTATTTTATTATAAGTATATTTATTTTTAAT‑AAAAATGTAAAAGAAATTTTATTTTAATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACttt            <  1:15562/128‑1 (MQ=255)

                                                                                                                            tttttttt‑‑‑ATATAAA‑TATAATTTATTTGATTAATAGTATATTTATGTTTAAT‑TAAAATTGAAAAGAAAGTGAA‑TGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACttttt          <  1:1822036/128‑1 (MQ=255)

                                                                                                                             ttttttt‑‑‑ATATATA‑TATTATTTTTTTGATTTATTGTATTTTTATGTTTAAT‑AATAATGGAAAAGAAAGTTATTTGTAATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACttttt          <  1:3352640/128‑1 (MQ=255)

                                                                                                                                 ttt‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACTTTTTTGtt      <  1:4040824/128‑1 (MQ=255)

                                                                                                                                      ttcAAAAA‑TATAATTTATTTGATTAAAATTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACTTTTTTGtttt    <  2:3479917/126‑1 (MQ=255)

AAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAA‑TTGTTAAATTATTTTCAAAT‑CATTA‑TCTATTAATCAA‑TTTTATGCTC‑G‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAA‑TATAATTTATTTGATTAAACGTATATTTATGTTTAAT‑AAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAA‑TTAAATATATAACAATGTAAGTTTTAAAACTTACTTTTTTGTTTTTA  >  CP016816/420128‑420380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`