Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.72.8% 394.4 / inf 386intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (12/78);  new base . (108/133);  total (139/247)
Fisher's exact test for biased strand distribution p-value = 4.06e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-01

Only 100 of 415 total aligned reads displayed.

GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTTTTAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAA  >  CP016816/202357‑202609
                                                                                                                                  |                                                                                                                              
gAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑‑AAAAAAAAACAATCCCCt                                                                                                                                <  2:304611/128‑1 (MQ=255)
  attttttgtAAATAATAGAGTTAATGTTAATTAAAATTCCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCtt                                                                                                                               <  1:1068333/122‑1 (MQ=255)
  aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAAAAAAACCCtt                                                                                                                               >  1:2423656/1‑128 (MQ=255)
   tttATTGTAAATAATAGAGTTAAGGTTAATTAAAATAACATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATATTCACAAAT‑AAAAAAAAAACAATCCCCtta                                                                                                                              <  1:1889196/127‑2 (MQ=255)
         gtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCACTTaaaa‑a‑at                                                                                                                      >  1:4230726/1‑127 (MQ=255)
            aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATTTATATACTATATATATATTTTATTAA‑TTTTTTATATTCACAAAT‑AAAAAAAAAACAATACCAttaaaaattaaa                                                                                                                     >  2:92315/1‑118 (MQ=255)
            aataatAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAAAAATACCAttaaaaattaaa                                                                                                                     >  1:4519748/1‑118 (MQ=255)
                 taatgttttgtttaattaaAATTAAAAAAAATTTATTATTATTTTAATGTATTTCACTGTTTTATATTTTTTTTT‑TTTTTTATATTAAAAAAA‑AAAAAAAAAACAATCCCCTTT‑‑A‑A‑AATTaaaaaaaa                                                                                                            <  1:3528670/117‑1 (MQ=255)
                 taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAATAAAAAAAAAAACAATCACC‑‑ttaa‑a‑aattaaaaata                                                                                                             >  2:3890094/1‑126 (MQ=255)
                  agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATACTCGTGATCTATTATTTTAT‑TTGTATATCTTCACAAAT‑AAAAAAAAAACAATCCAC‑‑‑AAA‑A‑AAATtaaaaaaaaa                                                                                                          >  2:203308/1‑128 (MQ=255)
                  agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaa                                                                                                           >  1:4366171/1‑128 (MQ=255)
                     gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAAAAATCACCTT‑‑‑A‑A‑AAATaaaaaaaaaaaaa                                                                                                       >  2:3453644/1‑128 (MQ=255)
                     gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTAACTTCACAAAT‑AAAAAAAAAAAAATAAAC‑‑ATAA‑A‑AAATaaaaaaaaaaaa                                                                                                        >  2:2240283/1‑128 (MQ=255)
                     gTTAAGGTTAATTAAAATACCATAAACTAAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAATAAAAAAAAAAACAATCCC‑‑‑AAAA‑A‑AAATaaaaaaaaaaaa                                                                                                        >  1:3107526/1‑128 (MQ=255)
                      ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaa                                                                                                       >  2:3702939/1‑128 (MQ=255)
                      ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATAAC‑‑ATTAA‑A‑AATTaaaaaaaaaaaaa                                                                                                       >  2:4456498/1‑128 (MQ=255)
                      ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑ATGTTTATCTTCACAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaa                                                                                                       >  1:4492178/1‑128 (MQ=255)
                       tAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaa                                                                                                      >  1:3776911/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTAAT‑ATGTTTATCTTCACAAAA‑AAAAAAAAAAAAATACAC‑‑ATAA‑A‑AATTaaaaaaaaaaaaaaa                                                                                                     >  2:4217448/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCACC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaa                                                                                                     >  1:3933503/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑ATGTTTATCTTCACAAAT‑AAAAAAAAAACAATCACC‑‑‑TAA‑A‑AAATtaaaaaaaaaaaaaaa                                                                                                    >  1:1726380/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTATTTAT‑TTGTTTATCTTCACAAAT‑‑AAAAAAAAACAATCCAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaa                                                                                                    >  1:3568338/1‑128 (MQ=255)
                        aaGGTTAATTAAAATACAATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTTTTTATCTTCACAAAT‑AAAAAAAAAAAAATCCAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaa                                                                                                     >  1:2363633/1‑128 (MQ=255)
                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCAAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaa                                                                                                    >  2:2353481/1‑128 (MQ=255)
                         aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCAAAAAT‑AAAAAAAAAAAAAAACCC‑‑TTAA‑A‑AAATaaaaaaaaaaaaaaaa                                                                                                    >  1:1014407/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAATTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAAACCCC‑‑‑TAA‑A‑AAATttaaaaaaaaaaaaaaaa                                                                                                  >  1:4113231/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTTATCTAAATTATTAT‑TATAATATCTACACAAAT‑AAAAAAAAAAAAAAAAAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaa                                                                                                   >  1:924567/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTGT‑ATTTTTATCTTCACAAAT‑AAAAAAAAAAAAAATAAC‑‑ATAA‑A‑AAATtaaaaaaaaaaaaaaaa                                                                                                   >  2:1270883/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTAT‑TTTTTTATATTAAAAAAT‑AAAAAAAAAAAAA‑‑AAAAATTAA‑A‑AATTaaaaaaaaaaaaaaaaa                                                                                                   >  2:1358843/1‑128 (MQ=255)
                          ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAATAAAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaa                                                                                                    >  1:3868150/1‑128 (MQ=255)
                           gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTTTTTATATTAACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaa                                                                                                  >  2:470120/1‑128 (MQ=255)
                           gTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaa                                                                                                  >  2:3982529/1‑128 (MQ=255)
                           gTTAATTAAAATAACATAAAAATAAAAAATAAAAAAAATAATAACCCCGTTAACTAATTTTTTAT‑TTTTATATATACACAAAA‑AAAAAAAAAACAAACCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaa                                                                                                  <  2:1231570/128‑1 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATAACC‑‑ATAA‑A‑AATTaaaaaaaaaaaaaaaaaaaa                                                                                                >  1:2607028/1‑128 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAAAACCCTaaaaa‑a‑aaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  1:2907970/1‑128 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATATTCACAAAT‑AAAAAAAAAACAATCCCC‑‑‑TAA‑A‑AAATtaaaaaaaaaaaaaaaaaaaa                                                                                               >  1:3611916/1‑128 (MQ=255)
                             taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAA‑TTATTTATATTCACAAAT‑AAAAAAAAAAAAAAACCCAaaaaa‑a‑aaaaaaaaaaaaaaaaaaaaaa                                                                                                  >  2:2400814/1‑128 (MQ=255)
                             taattaaAATACCATAAACTTAATAAATCTTATAATGTATATACTCTTGATCTATATTTTTAT‑TTGTTTATCTTAACAAAT‑AAAAAAAAAAAAAAAAAATT‑‑‑A‑A‑AAATaaaaaaaaaaaaaaaaaaaaa                                                                                               >  2:2720797/1‑128 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTATATCCTCTTGATCTATTTTTTTAA‑TTGAATATCATCAAAAAT‑AAAAAAAAAAAAATCAC‑‑‑ATAA‑A‑AAATaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:1332652/1‑127 (MQ=255)
                              aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTATAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAAACACC‑‑TAAA‑A‑AATTaaaaaaaaaaaaaaaaaaaaa                                                                                               >  1:566975/1‑128 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAACCAATACAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaacaaaca                                                                                              >  1:2308361/1‑126 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:4026748/1‑127 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                              >  1:4192268/1‑127 (MQ=255)
                               attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAAACACAATAAAA‑A‑TAaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  1:2155504/1‑128 (MQ=255)
                                  aaaaaaACAAAAACTTAAAAATTTTTAAATATTATATACTCTTTTTATTTTTTTTTTT‑TTTTTTAATTTAAAAAAA‑AAAAAAAAAAAAAAACCC‑‑ATAA‑A‑AAAT‑AAAAAAAAAAAAAAAAAAAACTaaa                                                                                          <  1:3851820/128‑1 (MQ=255)
                                  aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTATTATAT‑TTTTATATCTTCACAAAT‑AAAAAAAAAACAATAAC‑‑ATAAA‑A‑AATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:2957417/1‑124 (MQ=255)
                                   aaaTACCATAAACTTAATAATTCTTATAAAGTAGATCCTCGTGATCTATTTTTATAT‑TTGTATATCTTCACAAAT‑AAAAAAAAAACAAAACAC‑‑TTAA‑A‑AATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:2647244/1‑123 (MQ=255)
                                    aaTACAATAAAATTAATAATTTTTTTAATTTTTATTATTTTTTTTTTTTTTTTTAT‑TTTTTTTTTTTAAAAAAT‑ATAAAAAAAAAAATACCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTaaaa                                                                                         <  1:671225/128‑1 (MQ=255)
                                      tACCATAAAATTAATAAATATTAAAATTTTTATCCTTGTTTTATATTTTTTTAT‑TTTTTTATATTAAAAAAA‑AAAAAAAAAAAAAACCCC‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTaaaaa                                                                                        <  2:3801282/128‑1 (MQ=255)
                                        acATAAAATTAATAATTCTTATAATTTTTATACTATTTTTATTTTTTTTTAT‑TTTTTTTTCTTAAAAAAT‑AAAAAAAAAACAATCTCATT‑‑‑A‑A‑AAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAActc                                                                                 <  1:4087675/127‑1 (MQ=255)
                                        aaaaaaaaTTAAAAATTTTTATAATTTTTATCATATTTTTATATTTTTTTTT‑TTTTTTTTATTCAAAAAA‑AAAAAAAAAAAAATCCCC‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                      <  1:1654674/124‑1 (MQ=255)
                                        aaaaaaaCTTAAAAATTATTATAATTTATATAATCTTTTATTATTTTTTTAT‑TTTTTTAAATTAAAAAAA‑AAAAAAAAAAAAAACCCC‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                      <  2:2506967/124‑1 (MQ=255)
                                             aaaataataaATATTATAATGTATATCCACCTGATATATTTTTTTAT‑TTTTTTATATTAACAAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                               <  2:4038178/124‑1 (MQ=255)
                                              aattAATAATTATTATAATTTATATAATATTGTTATATTTTTTTAT‑TTTTTTATATTAAAAAAA‑AAAAAAAAAACAAACCCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                               <  2:1073690/126‑1 (MQ=255)
                                                      aatattATAATTTATATCCTCGTTTTTTATTTTTTTAT‑TTTTTTATCATCACAAAA‑AAAAAAAAAAAAAACCCCTTAAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCtt                                                                         <  2:4195977/124‑1 (MQ=255)
                                                       tttttATAATTTAGATACTTGTTTTTTATTTTTTTAT‑TTTTTTATCTTAAAAAAT‑AAAAAAAAAAAAATACCCTT‑‑‑A‑A‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                    <  2:4122518/125‑1 (MQ=255)
                                                       ttattATAATTTATATAATCGTTTTTTATTTTTTTAT‑TTTTTTATATTAAAAAAT‑AAAAAAAAAAAAAAACCC‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                                       <  1:3467847/125‑1 (MQ=255)
                                                          taaaaaTTTAGAAACTTGTGATATTTTTTTTTTT‑TTTTTTATATTAAAAAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTc                                                                  <  2:1525284/124‑1 (MQ=255)
                                                           tataATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACACTCCCC‑‑‑TGA‑A‑AAAT‑TAAAAAAAAAAAAAAAAAAACAAAAAACAAACAATATTTCTAAAAAATcac                                                                >  1:4262252/1‑126 (MQ=255)
                                                             taAAGTAGATCATATTTATATATTTTTTTAT‑TTTTTTTTCTTAACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCt                                                               <  2:1615650/128‑1 (MQ=255)
                                                                   ttaTCCTCGTGTTATTTTTTTTTAT‑TTTTTTAAATTAACAAAA‑AAAAAAAAAAAAAAACCC‑ATAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatta                                                           <  2:579645/126‑1 (MQ=255)
                                                                   atataatCGTTATATATTTTTTTAT‑TTTTTTAAATTAAAAAAT‑AAAAAAAAAAAAATACCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtattat                                                          <  2:1141826/122‑1 (MQ=255)
                                                                       ccTCGTTATCTTTTTTTTTAT‑TTTTTTTTCTTAAAAAAA‑AAAAAAAAAACAAACCCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                      <  2:3758911/128‑1 (MQ=255)
                                                                       atttttatttattttttttat‑tt‑tttttatTAAAAAAA‑AAAAAAAAAAAAATACCCTTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                       <  2:4358255/116‑1 (MQ=255)
                                                                        cattttatatttttttttat‑ttttttaTATTAACAAAA‑AAAAAAAAAAAAATAACC‑‑TTAA‑A‑AATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                     <  2:1584294/117‑1 (MQ=255)
                                                                        atctttattatttttttttt‑tttttTAAAATTAAAAAT‑AAAAAAAAAAAAATACCCTTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                       <  2:2449379/117‑1 (MQ=255)
                                                                          tgtttattttttttttAT‑TT‑TTTAAAATAAAAAAA‑AAAAAAAAAAAAAACACCTTAAAA‑ATAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                     <  2:4264790/119‑1 (MQ=255)
                                                                           tttttaaatttttttat‑ttttttATATTAAAAAAA‑AAAAAAAAAAAAAAACCCTTAAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                                    <  2:251996/121‑1 (MQ=255)
                                                                           tttatatatttttttat‑tttttTAAATAAAAAAAA‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                  <  2:3338378/122‑1 (MQ=255)
                                                                            ttatttatttttttat‑tttttTAAATTAACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                 <  2:1768176/123‑1 (MQ=255)
                                                                            cttatttattttttATATTTTATATATTCTAAAAA‑AAAAAAAAAAAAAAACCCATT‑‑A‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                                  <  1:460843/120‑1 (MQ=255)
                                                                              atatatttttttat‑tttttAATAATAAAAAAA‑AAAAAAAAAAAAATACCCTTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                 <  2:4230726/125‑1 (MQ=255)
                                                                               tatatttttttat‑tttttTATATTAACAAAA‑AAAAAAAAAAAAAACCATTAAAAATA‑AAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                                 <  2:4077076/126‑1 (MQ=255)
                                                                                 tatttttttat‑tttttTATATTAACAAAT‑AAAAAAAAAAAAATACCATT‑‑‑A‑A‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                          <  2:519172/128‑1 (MQ=255)
                                                                                 attttttttAT‑TT‑TTTATATTAAAAAAA‑AAAAAAAAAAAAAAACCCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                          <  2:81244/126‑1 (MQ=255)
                                                                                  ttttttttAT‑TT‑TTTATATTAAAAAAA‑AAAAAAAAAAAAATTCCCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                         <  2:41814/127‑1 (MQ=255)
                                                                                  ttttttttAA‑TTTTTTATCTTAACAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                           <  2:3590036/127‑1 (MQ=255)
                                                                                    ttttttat‑ttttttatTTAAAAAAAT‑AAAAAAAAAAAAAAACCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                         <  1:3486265/128‑1 (MQ=255)
                                                                                     tttttAT‑TTTTTTATATTAAAAAAA‑AAAAAAAAAAAAA‑‑AACAATTAA‑A‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                        <  1:3414657/128‑1 (MQ=255)
                                                                                      tttttt‑ttttttATTTTAAAAAAA‑AAAAAAAAAAAAAAACCC‑‑ATAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                       <  1:1096133/128‑1 (MQ=255)
                                                                                      tttttt‑ttttttATATTTA‑AAAA‑AAAAAAAAAAAAAAAAACATTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                        <  2:454185/128‑1 (MQ=255)
                                                                                      ttttAT‑TTTTTTATATT‑AAAAAA‑AAAAAAAAAAAAATACCC‑‑TTAA‑A‑AAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAAt                                     <  1:3717520/128‑1 (MQ=255)
                                                                                       tttAT‑TTTTTTATATTAAAAAAT‑AAAAAAAAAAAAAAACACCTTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                        <  2:3520599/128‑1 (MQ=255)
                                                                                             ttttttATCTTAAAAAAA‑AAAAAAAAAAAAATCACCTTAAAA‑A‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                                   <  2:3611916/125‑1 (MQ=255)
                                                                                             ttttttATATTAAAAAAA‑AAAAAAAAAAAAAAACCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                                 <  1:3657769/125‑1 (MQ=255)
                                                                                                ttaaaaataaaaaaa‑aaaaaaaaaaaaATCAC‑‑ATTAA‑A‑AAA‑AAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                             <  2:2182362/117‑1 (MQ=255)
                                                                                                  ttaattaaaaaaa‑aaaaaaaaaaaaATAACC‑TTAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                             <  2:3229023/119‑1 (MQ=255)
                                                                                                  tatttaaaaaaaT‑AAAAAAAAAA‑AATAATCTATAAA‑A‑ATAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                            <  1:3602353/119‑1 (MQ=255)
                                                                                                  tataataacaAAA‑AAAAAAAAAAAAAAACC‑‑ATTAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                            <  1:1588807/121‑1 (MQ=255)
                                                                                                   tttttaaaaaaa‑aaaaaaaaaaaaATACC‑‑ATTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                           <  2:1710309/120‑1 (MQ=255)
                                                                                                    atttttaaaaT‑AAAAAAAAAAAAAATTCCAATAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTg                            <  1:518498/121‑1 (MQ=255)
                                                                                                    acttaaaaaaa‑aaaaaaaaaaaaATCACCTT‑‑‑A‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                         <  2:1466731/121‑1 (MQ=255)
                                                                                                     ttataaaaaa‑aaaaaaaaaaCAAAAACCTAAAAA‑A‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                           <  1:4149535/122‑1 (MQ=255)
                                                                                                     cttataaaaT‑AAAAAAAAAAAAATAGAC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAAAAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                         <  2:1953107/122‑1 (MQ=255)
                                                                                                      tttaaaaaT‑AAAAAAAAAAAAATATCC‑TTTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                         <  1:2720797/123‑1 (MQ=255)
                                                                                                        aaaaaaa‑aaaaaaaaaaaaaaACCC‑‑TTAA‑A‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                      <  1:3999052/125‑1 (MQ=255)
                                                                                                          caAAA‑AAAAAAAAAAAAAACCCC‑‑TTAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata                    <  2:3728044/128‑1 (MQ=255)
                                                                                                           aaaa‑aaaaaaaaaaaaaaaaCACTTAAA‑A‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataat                     <  1:2986514/128‑1 (MQ=255)
                                                                                                             aaaaaaaaaaaaaaaATCCCC‑ATAAA‑A‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatat                   <  2:3857355/125‑1 (MQ=255)
                                                                                                                 aaaaaaaaaaaaTACCC‑‑TTAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATc              <  2:1906583/128‑1 (MQ=255)
                                                                                                                                  |                                                                                                                              
GAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAT‑TTGTTTATCTTCACAAAT‑AAAAAAAAAACAATCCCCTTTTAA‑A‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATATATAA  >  CP016816/202357‑202609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑