BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	20,023	2 bp→TA	intergenic (‑744/‑392)	JCVSYN2_00065 ← / → JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	20,023	0	A	T	99.6%	12565.0 / NA	4234	intergenic (‑744/‑393)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base A (9/10); new base T (2336/1877); total (2347/1887)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	20,024	0	T	A	99.6%	12715.3 / NA	4232	intergenic (‑745/‑392)	JCVSYN2_00065/JCVSYN2_00070	hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand): ref base T (9/7); new base A (2335/1880); total (2345/1887)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4288 total aligned reads displayed.

GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19899‑20145

||

   cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      >  2:145387/1‑128 (MQ=255)

    aGGAAGAGCAAGTAAAAAGGTAGTGTTTGTTGGCGATGCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     <  1:6626556/128‑1 (MQ=255)

    aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCTCCTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     <  2:4268560/128‑1 (MQ=255)

     ggAAGATCAAGAGAAAACGTAGTATTTGTTGGCGCTCCCCCTAGAGTCTTTTACATCATCGGAAAACAAAAACTATTTTTTCTTTAACTTC‑TTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    <  1:8417079/128‑1 (MQ=255)

       aaGAGCAAGATAAAAGGTAGTATTTGTTTGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTAATTTTACTTTAATTTC‑TTTTATTACTTTCTATTTTTAATTTATATATTTataata                                                                                                                  >  2:6520593/1‑128 (MQ=255)

           gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaa                                                                                                              >  1:3648394/1‑128 (MQ=255)

            cAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaaa                                                                                                             >  2:674358/1‑128 (MQ=255)

               gATAAAAGGTAGTATTTGTTGGCGACCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                          <  2:2298249/128‑1 (MQ=255)

                 tAAAAGGTCGTATTTGGTGGCGAGCCCCCTAGAGTCTATTACATCATCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        <  1:7542136/128‑1 (MQ=255)

                     aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                    >  2:7546376/1‑128 (MQ=255)

                      ggTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAATCAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAatttat                                                                                                   >  2:3589592/1‑128 (MQ=255)

                       gtattaGTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattata                                                                                                  <  1:1551956/128‑1 (MQ=255)

                       gtagtaTTTGTTGGCGATCCACCTAGAGTCTTTTAAATATTCGTAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattata                                                                                                  <  1:10362037/128‑1 (MQ=255)

                       ggagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattata                                                                                                  <  1:5583460/126‑1 (MQ=255)

                        tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 >  2:1649342/1‑128 (MQ=255)

                        tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 <  1:1747082/128‑1 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  2:7997834/1‑128 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  1:2357177/1‑128 (MQ=255)

                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATATTCGGAAAACAAAAAATATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  2:5166401/122‑1 (MQ=255)

                             tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            >  2:1888314/1‑128 (MQ=255)

                             tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            <  2:4889846/128‑1 (MQ=255)

                              ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttt                                                                                           >  2:8575515/1‑128 (MQ=255)

                               tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAtttt                                                                                          <  1:8020344/128‑1 (MQ=255)

                                    gCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                     <  1:5106019/128‑1 (MQ=255)

                                    gCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                     >  1:5254157/1‑128 (MQ=255)

                                    gCGATCCCCCTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                     <  2:10330084/128‑1 (MQ=255)

                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:9372567/1‑128 (MQ=255)

                                     cGATCCCCCTAGAGTCGTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  1:7857974/128‑1 (MQ=255)

                                      gATCCCCCTAGAGTCTTTTTCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  2:4463129/128‑1 (MQ=255)

                                      gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    <  2:8730628/128‑1 (MQ=255)

                                      gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                   <  2:815316/128‑1 (MQ=255)

                                        tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                 <  1:8623512/128‑1 (MQ=255)

                                            tgtAGATTGTTTAAGATGTTCTGAAAAAAATTTCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCAATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  2:3095392/126‑1 (MQ=255)

                                            ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             >  1:6309470/1‑128 (MQ=255)

                                            ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             <  1:7705370/128‑1 (MQ=255)

                                               agagTCTTTTACATCTTCTGAAATCAAAATCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATAATAATAATTTTAATAGCACGTGATAAt                                                                          >  1:5438281/1‑128 (MQ=255)

                                                  gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  2:692499/104‑1 (MQ=255)

                                                    cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     >  2:7252324/1‑128 (MQ=255)

                                                     ttttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAg                                                                    <  1:1368272/128‑1 (MQ=255)

                                                        tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 <  2:10172954/128‑1 (MQ=255)

                                                        tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTa                                                                 >  1:4310047/1‑128 (MQ=255)

                                                        tACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGATa                                                                 >  1:3468098/1‑128 (MQ=255)

                                                         aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  1:9060987/128‑1 (MQ=255)

                                                          cATCTTCGGAAAACAAATACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                               <  2:2898252/128‑1 (MQ=255)

                                                          cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATAAAAAGTTAGt                                                               >  2:2507625/1‑128 (MQ=255)

                                                           atattaggAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTg                                                              <  2:2097351/122‑1 (MQ=255)

                                                           aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTATTATAGCACGTGATAATTAAAAGTTAGTg                                                              >  1:9958722/1‑128 (MQ=255)

                                                            tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                             <  1:486115/128‑1 (MQ=255)

                                                             cTTCGGAAAACAAAAACTATTTTTTCTTTATTTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGaa                                                            <  1:9600382/128‑1 (MQ=255)

                                                             cTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAATAGTTAGTGaa                                                            >  2:467591/1‑128 (MQ=255)

                                                               tCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          <  1:4144610/128‑1 (MQ=255)

                                                               tCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                          >  2:898708/1‑128 (MQ=255)

                                                                cGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                         <  1:1187349/128‑1 (MQ=255)

                                                                 ggAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                        <  1:6140536/128‑1 (MQ=255)

                                                                 ggAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                        >  1:5742925/1‑128 (MQ=255)

                                                                   aaaaCAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAATTTATTGAACAAGaa                                                      >  2:4155529/1‑128 (MQ=255)

                                                                   aaaaCAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      <  2:3289621/128‑1 (MQ=255)

                                                                     aaCAAAAACTATTTTTTCTTTAATTTC‑TATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAACTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaaa                                                    <  1:9756146/128‑1 (MQ=255)

                                                                         aaaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  1:10211942/1‑128 (MQ=255)

                                                                         aaaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  1:8221343/1‑128 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCGCGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               >  1:6800965/1‑128 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               >  1:10000339/1‑128 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  2:6377152/128‑1 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAAATTAAAGTTAGAGAAAAAGAAAACAGTg                                               >  2:4003625/1‑128 (MQ=255)

                                                                          aaaCTATTTTTTCTTTAATTTC‑TTTATTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTAAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                               <  1:4501841/128‑1 (MQ=255)

                                                                           aaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              >  1:9429951/1‑128 (MQ=255)

                                                                           aaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              <  1:1048588/128‑1 (MQ=255)

                                                                           aaCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              >  1:307287/1‑128 (MQ=255)

                                                                            aCTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGATAACAGTGaa                                             >  2:1063814/1‑128 (MQ=255)

                                                                            aCTATTTTTTCTTTAATTTA‑TTTTTTTACTTTCTATTTTTAATTTATAAATTTATATTAAAAAATTTAATTTATAATTATTTATATAGAAATTGATAATTAAAAGTTAGTGAAAAATAAAACAGTaaa                                             >  1:2527317/1‑128 (MQ=255)

                                                                             cTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            >  2:5490968/1‑128 (MQ=255)

                                                                             cTATTTTTTCTTTAAATTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  2:2695404/128‑1 (MQ=255)

                                                                              tATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           <  2:5953846/128‑1 (MQ=255)

                                                                                ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                         >  1:4661143/1‑112 (MQ=255)

                                                                                ttttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  1:290393/1‑128 (MQ=255)

                                                                                 tttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                        >  2:9731279/1‑128 (MQ=255)

                                                                                  ttttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                       <  2:5300714/128‑1 (MQ=255)

                                                                                    ttCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGt                                     <  2:1280179/128‑1 (MQ=255)

                                                                                     tCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTTAACAAGAAAACAGTGAAGCACCTGtt                                    >  1:6983570/1‑128 (MQ=255)

                                                                                      cTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:8374341/1‑128 (MQ=255)

                                                                                         tattttA‑TTTTTTTAATTTCTATTTTTAATTTATATATTTATATTAAAAAATTTATATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                <  1:2137204/125‑1 (MQ=255)

                                                                                         tAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTg                                >  2:6170486/1‑128 (MQ=255)

                                                                                          aaTTTC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGa                               <  2:37839/128‑1 (MQ=255)

                                                                                            tttC‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                             >  1:7161404/1‑128 (MQ=255)

                                                                                               c‑TTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACTAGAAAACAGTGAAGCACAAGTTTCTGAACCaa                          >  2:7078158/1‑128 (MQ=255)

                                                                                                    ttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaaga                      >  1:383102/1‑128 (MQ=255)

                                                                                                      ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    >  1:8889881/1‑128 (MQ=255)

                                                                                                      ttACTATCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                    <  2:573152/128‑1 (MQ=255)

                                                                                                       tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagcaga                   >  1:590304/1‑128 (MQ=255)

                                                                                                        aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTTAACAAGAAAACAGTGAAGCACCAGTTACTGAACCAAAAGAAGAc                  >  2:3450016/1‑128 (MQ=255)

                                                                                                        aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAACAAAAGAAGAc                  >  1:1943279/1‑128 (MQ=255)

                                                                                                         cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 <  1:9239964/128‑1 (MQ=255)

                                                                                                         cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 <  1:6463174/128‑1 (MQ=255)

                                                                                                          tttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGa                <  2:9182702/128‑1 (MQ=255)

                                                                                                              tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGAACCAGTTTCTGATCCAAAAGAAGACGaaaaa            >  2:7776861/1‑128 (MQ=255)

                                                                                                               aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           >  1:4000701/1‑128 (MQ=255)

                                                                                                                tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc          >  1:2729352/1‑128 (MQ=255)

                                                                                                                 ttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAATAACCAGTTTCTGAACCAAAAGAAGACGAAAAAACa         >  1:3292290/1‑128 (MQ=255)

                                                                                                                   ttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaa       >  2:9560029/1‑128 (MQ=255)

                                                                                                                       tttATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaaa   <  1:7357609/128‑1 (MQ=255)

||

GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19899‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`