Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2530T.44.2% 433.1 / inf 345intergenic (+217/‑325)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (92/76);  new base . (107/26);  total (201/144)
Fisher's exact test for biased strand distribution p-value = 2.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-02

Only 100 of 471 total aligned reads displayed.

ATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTAAAAGAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGTTAATTAAAACTTA  >  CP016816/165126‑165380
                                                                                                                                   |                                                                                                                                
  atttatttTTATTAATTTATAGATCTTTTATTTATTAGTTAGAAACTAAAAATATTATATTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTTAAAAATTAA‑TTTTAACTTGTAAAAAATTTCtttttt                                                                                                                                <  1:1842795/128‑1 (MQ=255)
  aattACTTTTATTAATTTATAGCAATTGTATTAATTAGTAACAAAATAAAAATATTATCTTTTTACA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATATCtttttt                                                                                                                                <  1:2172598/126‑1 (MQ=255)
     tattttCATTAATTTATAGCACTTGTATGAATTAGTAAGAAAAAAAAACTATTATCTTTTTAGA‑TA‑TTTTTTTTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttt                                                                                                                             <  1:1305931/125‑1 (MQ=255)
     tAGGTTCATTAATTTATCGCACTTGTATTTATTACTAATAAAATAATAATTATATATTTTTTTA‑TA‑TTTTTTTTAAATTCAAA‑TTTTTAAAAAATTAA‑TTTGCACTTGTAAAAAATACCttttttttt                                                                                                                             <  1:1001110/128‑1 (MQ=255)
      aggttCAATAAATTTTCTCACTTGTATGAATTATTAAGAAACTAAAACATTTATCTTTTTAGA‑TA‑TTTTCTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACCTGTAAAAAATAGCtttttttttt                                                                                                                            <  2:469565/125‑1 (MQ=255)
       tttaaaTTAATTTTTCTCAATTTTATTAATTATTAACAAAAAAAAAATATTATATTTTTATA‑TA‑TTTTTTTTATATGAAAA‑TTTTTAAAAAATTAA‑TTTTAACTTGTAAAAAATAGCttttttttttt                                                                                                                           <  1:873459/123‑1 (MQ=255)
            aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                                                      >  2:981985/1‑128 (MQ=255)
              tAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttttttttta                                                                                                                    >  1:650788/1‑127 (MQ=255)
               aaTTTATTGCAATTTTATGAATTATTAATAAAATAAAACTATTATATTTTTATA‑TT‑TTTTTTTTATATGAAAA‑TTTTTAAAAAATTAA‑TTTGAACTTTTAAAAAATATCttttttttttttttttata                                                                                                                   <  1:1158714/128‑4 (MQ=255)
                 tttATCTCAATTTTATTAATTATTAATAAAATAAAACTATTATCTTTTTATA‑AA‑TTTTTTGTATATTCAAA‑TTTTTAAAAAATTAA‑TTTTAACTTGTAAAAAATAGCtttttttttttttttataatg                                                                                                                 <  2:2246823/128‑7 (MQ=255)
                 tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttttttttataat                                                                                                                 >  2:2109351/1‑123 (MQ=255)
                 tttAACGCACTTGTATTAATTAGTAACAAAATAAAAATTATATCTTTTTATA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATATCtttttttttttttttataatg                                                                                                                 <  1:2112243/128‑7 (MQ=255)
                  ttATTGCACTTGTATTAATTAGTAATTAACTAAAAAT‑TTATCTTTTTTTT‑TA‑TTTTTTGTATATTCAAA‑TTTTTAAAAAATTAA‑TTTTTACTTTTAAAAAATAGCtttttttttttttttataatgga                                                                                                               <  1:70276/128‑9 (MQ=255)
                  ttATAGCACTTTTATGAATTATTAATAAACAAAAAATATTATATTTTTATA‑TA‑TTTTTTTTATATTCAAA‑TTTTTAAAAAATTAA‑TTTTAACTTGTAAAAAATAGCtttttttttttttttataatgg                                                                                                                <  1:1405393/128‑8 (MQ=255)
                    aTCGCCCTTGTATGAATTAGTAATAAAAAAAAAATATTATATTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAAATAA‑TTTTAAATTTTAAAAAATATCttttttttttttttttataatgga                                                                                                              <  1:1696364/128‑9 (MQ=255)
                         atttttATTAATAATTAAAAAAATAAAAAAA‑TATATTTTTATA‑TATTTTTTTTTATATTAAAT‑TTTTTAAAAAATTAA‑TTTTAAATT‑TAAAAAAAA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                                                    <  2:1261546/123‑1 (MQ=255)
                          cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAatat                                                                                                      >  2:2659206/1‑128 (MQ=255)
                           ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttttttttatttgaaatattaat                                                                                                       >  1:618740/1‑116 (MQ=255)
                            tttattaattattaatAAACTAAATATATTATCTTTTTTTA‑TA‑TTTTTTTTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                                   <  1:835946/126‑1 (MQ=255)
                            tGTTTGAATTTTTAATTAAATAAAACTATTATATTTTTATT‑TA‑TTTTTTGTATATGAAAA‑TTTTTAAAAAATTAA‑TTTGAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                                   <  1:578191/128‑1 (MQ=255)
                             ttttttattattAATAAAATAAAAATATTTTATTTTTATA‑TT‑TTTTTTTTTTATGCAAA‑TTTTTAAAAAATTAA‑TTTTAAATTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                 <  1:3096884/122‑1 (MQ=255)
                             tttttaaTTATTAATTAAATAAAACTTTTTACTTTTTATT‑TA‑TTTTTTTTATATTCAAA‑TTTTTAAAAAATTAA‑TTTTAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                 <  1:785937/123‑1 (MQ=255)
                              tttttattattAATTAAATAAAACTTTTATATTTTTATT‑TT‑TTTTTTTTATATTCAAA‑TTTTTAAAAAAATAA‑TTTTAAATTTTTAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                 <  1:3183336/123‑1 (MQ=255)
                                agtaTTAGTAATAAACTACAACTTAAATATTTTTAGA‑TA‑ATTTTTGTATTTTAAAA‑ATTTTAAAAAATTAA‑AATTAACTTTGAAAAAATA‑‑‑‑TCCTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                              <  2:827626/125‑1 (MQ=255)
                                   attattAATAAACTAAAACATTTATCTTTTTTTA‑TA‑TTTTTTGTATATTCAAA‑TTTTTAAAAAAATAA‑TTTTAACTTGTTAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                           <  1:419775/128‑1 (MQ=255)
                                     tattaataaAATAAAAATATTTTATTTTTTTT‑TT‑ATTTTTTTATATTAAAA‑TTTTTTAAAAATTAA‑TTTTTACTTGTAAAAAATTGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                         <  1:1242751/125‑1 (MQ=255)
                                     tattaataaAATAAAAATATTTTATTTTTATA‑TA‑TTTTTTTTTTATTCAAA‑TTTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                         <  2:2897156/125‑1 (MQ=255)
                                       ttaataaAATAAAAATATTTTATTTTTATT‑TT‑TTTTTTGTTTTTGCAAA‑TTTTTTAAAAATTTA‑TTTTAACTTGTTAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                                                       <  2:109616/127‑1 (MQ=255)
                                       ttAAGAAACTAAAACTATTATCTTTTTATA‑TA‑TTTTTTTTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                         <  2:2786499/127‑1 (MQ=255)
                                          ataaaataaaattaTTTTTTTTTTTTA‑TA‑TTTTTTTTATATTAAAA‑TTTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                     <  1:953944/126‑1 (MQ=255)
                                          ataaaaaaaaaataTTTTATTTTTATA‑TA‑TTTTTT‑TAAATTTAAATTTTTTATAAAAATTA‑TTTTAAATTGTAAAAAATA‑‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                     <  1:895724/121‑1 (MQ=255)
                                           taaaataaaaataTTTTTTTTTTATT‑TA‑TTTTTTTTATATTAAAA‑TTTTTAAAAAATTAA‑TTTTAACTTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgt                                                                                    <  1:2495727/127‑1 (MQ=255)
                                                taaaaaatttattttttttta‑tt‑tttttTTTTTATTTAAA‑TTTTTTAAAAATTAA‑TTTTATCTTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                                                              <  2:3038059/120‑1 (MQ=255)
                                                 aaaactaTTTTATTTTTTTT‑TA‑TTTTTTGTTTTTTGAAA‑TTTTTAAAAAATTAATTTTTAAATTTTAAAAAA‑‑‑‑‑TTCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATaa                                                                             <  2:154558/128‑1 (MQ=255)
                                                  aaattaTTATTTTTTTTTT‑TT‑TTTTTTGTATATTTAAA‑TTTTTTAAAAATTAA‑TTTTTACATGTTAAAAA‑‑‑‑TTGGTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                            <  1:2016412/128‑1 (MQ=255)
                                                  aaactaTTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTA‑GTATtttttttgtaa                                                                              >  2:168115/1‑123 (MQ=255)
                                                   aaaattttattttttATATTA‑TTTTTTTTATATTCAAA‑TTTTTTAAAAATTAA‑TTTTTAATTTTAAAAAATTGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                            <  1:527494/118‑1 (MQ=255)
                                                    attattttGTTTTTTTA‑TA‑TTTTTTTTTATTGCAAA‑TTTTTTAAAAATTAT‑TTTTAACTTGAAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                                                          <  1:2050684/126‑1 (MQ=255)
                                                    aatattttatttTTATA‑TT‑TTTTTTTGTTATGCAAA‑TTTTTTAAAAATTAA‑TTTTAAATTTTAAAATATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                                                          <  1:1617777/126‑1 (MQ=255)
                                                      tattatCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTATTTTTTTTTATaatttt                                                                         >  2:1818556/1‑124 (MQ=255)
                                                       attattTTTTTATT‑TA‑TTTTTTTTTTATTAAAATTTTTAAAAAAATAAA‑TTTAAAATTTAAAAAAAT‑‑‑TCGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                                                         <  2:588630/128‑1 (MQ=255)
                                                       attatCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATTTAAATTTTTTTTTTTTTGTTGtatattttta                                                                        >  1:2254123/1‑122 (MQ=255)
                                                        ttatCTTTTTGGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTAATAATTGAAATTTAAATTTTAGGTATTTTGTTTTATAATTTTCTaa                                                                     >  2:694240/1‑128 (MQ=255)
                                                        ttatATTTTTAGA‑TT‑ATTTTTGTTAATTCAAA‑TTTTTAAAAAATTAA‑ATTGAACTTTTAAAAAA‑AAC‑‑‑GTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTa                                                                      <  1:1372045/128‑1 (MQ=255)
                                                         tatCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATTTATTTTTATGTATaataaacaaa                                                                     >  1:1977739/1‑120 (MQ=255)
                                                           tCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATAAAAATTTAAGTTATTTTTTTTTATAATTTTATaaaa                                                                   >  2:1353436/1‑128 (MQ=255)
                                                            cTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATaaaaaaaa                                                                 >  2:3096888/1‑128 (MQ=255)
                                                             tttttATA‑TT‑TTTTTTTTATATTAAAA‑ATTTTAAAAAATTTA‑TTTTTACTTTTAAAAAAT‑‑‑TTATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                                                                  <  2:2141242/128‑1 (MQ=255)
                                                             tttttAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATGTAAATTTAAATTTTAGGATTTTAGTTTACTAATTTTATaaaaaa                                                                 >  2:1703635/1‑128 (MQ=255)
                                                              ttttAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTT‑‑‑‑TAATTGAAATTAAAATTTTATTTTTTTTTTTGTAAAATTATATaaaaaat                                                                >  1:2184718/1‑127 (MQ=255)
                                                               tttAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTAGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTAATAATTGAAATATAAATTTTAGATAATTTGTTGTATAAATATATaaaaataa                                                               >  1:1443285/1‑125 (MQ=255)
                                                               tttAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACATGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAAATAAAATTTTAGTAATTTTTTTTTATAATTATCAAAAAAAATa                                                              >  1:1808029/1‑128 (MQ=255)
                                                                ttATA‑TA‑TTTTTTGTTTATTCAAA‑TTTTTAAAAAATTAA‑TTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                                <  1:2540094/128‑1 (MQ=255)
                                                                ttAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATGTAAATAAAAAATTTATTTATTTTTTTTTATAATTATATAAAAAAATat                                                             >  1:1725977/1‑127 (MQ=255)
                                                                 tAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAATat                                                             >  2:1141539/1‑127 (MQ=255)
                                                                 tAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGTTTTTTTTTTTTATAATTATTAAAAAAAATaa                                                             >  2:799523/1‑127 (MQ=255)
                                                                   gA‑TA‑TTTTTTGTTTTTTCAAA‑TTTTTTAAAAATTAA‑TTTGTAATTTTAAAAAA‑A‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                                                            <  1:2611392/128‑1 (MQ=255)
                                                                    ttta‑tTTTTTTTATTTTTAAA‑TTTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                             <  2:3194132/126‑1 (MQ=255)
                                                                       a‑tTTTTTGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTAAAATATAAATTTAAGTAATTTTTTTTTATAATTATATAAAAAAATATaaataa                                                       >  2:2038549/1‑125 (MQ=255)
                                                                        tttttttttttATTAAAA‑TTTTTAAAAAATTAA‑TTTTTATTTTTAAAAAA‑A‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTTGTTTTTTTTTTGTTTAATTATATAAAAAATTATaatga                                                        <  1:163382/127‑4 (MQ=255)
                                                                         ttttttGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTAATTTTTATGTAAAAGAAAATAAAAAAATAGAAAGGa                                                       >  1:2373413/1‑128 (MQ=255)
                                                                           ttttttttATGTAAA‑TTTTTAAAAAATTTT‑TTTTAAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAGTTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                                                     <  1:1537178/128‑1 (MQ=255)
                                                                           ttttGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATaaatta                                                       >  2:1995777/1‑125 (MQ=255)
                                                                            tttttttatTCAAA‑TTTTTTAAAAATTAA‑TTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                                                     <  1:2826815/122‑1 (MQ=255)
                                                                            tttGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAATTaaaa                                                    >  2:415079/1‑128 (MQ=255)
                                                                              tGTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTT‑‑‑‑‑‑TAATGTAAATAAAAATTTTAGTTTTTTTTTTTAAAAATAAACTAAAAAAATAAAAAGAaaaaaaaa                                                >  1:1588983/1‑126 (MQ=255)
                                                                               gTATATGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATATAAATTTTAGGTATTTTGTTGATAAATTATTAAAAAAAATTAAAAGTaaaaaa                                                  >  1:3211547/1‑128 (MQ=255)
                                                                                tataTGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATATTGGAAAATTAAATTTTTGGTTTTTTGTTGTAAAATTAACTAAAAAAATAGAAATCAAAAAata                                                >  1:2859558/1‑128 (MQ=255)
                                                                                   atGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTGTATAATTATATAAAAAAATATAAAGGAAAAAAtttttt                                            >  1:276101/1‑126 (MQ=255)
                                                                                    tGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTCTAATTTAATAAAAAAATATAAATAaaaaaaattttt                                            >  2:644359/1‑122 (MQ=255)
                                                                                    tGCAAA‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTGAAATATAAATTTTTAGTATTTTTTTTTTTTATTTTATAAAAAATAATAAATGAATAAATATtttt                                           >  2:1393764/1‑128 (MQ=255)
                                                                                       aaa‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTTTTAAATTATAAAAAAAAATAAAAATAAAAAAATATTTTTa                                          >  1:246735/1‑128 (MQ=255)
                                                                                       aaa‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTATTTTGTTTTAAAATTATATAAAAAAATATAAAGTAAAAAATATTTTTaa                                         >  1:2299966/1‑128 (MQ=255)
                                                                                       aaa‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTAAGTTATTTTGTTTTATAATTATCAAAAAAAAAATAATGTAAAAAAAATTATTaa                                         >  1:1186826/1‑128 (MQ=255)
                                                                                         a‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTGTATAAGTATGAAAAAAATTAGAAATAAAAAAATTATATTaaa                                        >  1:1745272/1‑128 (MQ=255)
                                                                                              tttaaaaattaa‑ttTTAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGAAAATATAAATTTTATGTATTTTTTTTTATAATTAACTAAAAAAATAGAAAGAATAAATAATTTTTAAAAATaa                                   >  2:1614677/7‑128 (MQ=255)
                                                                                              ttaaaaaaTTAA‑TTTGAACTTGTAAATAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATAAAAAAATAGAAAGTAAAAAATATTGTTAAAAGTaa                                   >  2:2446867/3‑128 (MQ=255)
                                                                                              ttaaaaaaTTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAATTATATAAAAAAATATAAATTAAAATATATTGTTAAAAGTa                                    >  2:1499531/3‑128 (MQ=255)
                                                                                               taaaaaaTTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAT‑‑TGTAAATATAATTTTTTGTTTTTTTTTTTTATAAGTAAAAAAAAAAATAAAAATAAAAAAATTTTTTTaaattta                                    >  2:2535949/2‑124 (MQ=255)
                                                                                                aaaaaaTTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTTTTTTTTTTTTTTTATAATTATATAAAAAAATATAAATTTAAATAAATTTTTaaattaaata                                 >  2:2361749/1‑121 (MQ=255)
                                                                                                 aaaaattaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAAAAAAAATTTTAGGTATTTTGTTTTCTAATTAAATAAAAAAAAATAAATGAAAAAATATTTTTAAAATTaaaaa                                >  1:2965985/4‑125 (MQ=255)
                                                                                                    aattaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATTATGTAAATAAAAATTTTTGTTATTTTGTTGTAAAATTATAAAAAAAAATAAAAATTAAAAAATTTTGTTAAAATTAAA‑ACaaa                            >  2:2981609/1‑128 (MQ=255)
                                                                                                    aattaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTAAAATTGAAATAAAAATTTTAGGTATTTTTTTGTATAAGTAACTAAAAAAAAAGAAAGTAAAAAATATTTATAAATGTAAA‑ACTaaa                           >  1:120635/1‑128 (MQ=255)
                                                                                                     attaa‑ttTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGGTATTTTGTTTTATAATTATATAAAAAATTATAAATGAAAAAATATTGTTAAATTtaat‑aataa                            >  2:1889605/1‑128 (MQ=255)
                                                                                                     attaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTAAAATATAAATTTTATGTATTTTTTTTAATAAGTAAATAAAAAAATAGAAAGGAAAAAATATTTTTaaaataaaaaataaa                            >  1:728464/1‑117 (MQ=255)
                                                                                                     attaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTATTTTTGTTGTATAATTATATAAAAAAATAAAAAGTAAAAAAAATTTTTAAaataaat‑aaaaaa                           >  1:2900794/1‑123 (MQ=255)
                                                                                                     attaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTGGAAAAGAAACAAAAAAAATACAAAGGAAAAAATATTGGTAAAAGAAAT‑AATaaaa                          >  1:1921292/1‑128 (MQ=255)
                                                                                                     attaa‑ttTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTT‑‑‑‑TAATTTAAATATAAATTTTTTTTTTTTTTTTTAAAAATTATATAAAAAAATAAAAATTAAAAAAATTTTTAAAAATTAAT‑AATaaa                           >  1:2240560/1‑128 (MQ=255)
                                                                                                      ttaa‑ttTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATTGAAATTTAAATTTTTTGTTATTTTTTTTATAATTTTATTAAAAAATTAAAATGAAAAAATTTTGTTAAAAAGAaa‑aataaaat                         >  2:2837459/1‑128 (MQ=255)
                                                                                                      ttaa‑ttTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTA‑AATGGAAATTTAATTTTAAGGTATTTTTTTTAATATGTATATAAAAAAATTTAATGGAAAAAATTTTTTTAAAAGTAAT‑ATTaaa                           >  2:886141/1‑128 (MQ=255)
                                                                                                      ttaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTATGTATTTTTTTGTATAAATATATAAAAAAAAAAAAATGAAAAAATATTTTTAAAATTAAA‑AATaaaa                          >  1:3021243/1‑128 (MQ=255)
                                                                                                      ttaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTCTAATTTTATAAAAAAATATAAATTAAAAAATATTGTTAAAATTAAT‑AATAAAat                         >  2:1197892/1‑128 (MQ=255)
                                                                                                                     aattttaaaaaatttgttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATg                <  1:1065882/116‑1 (MQ=255)
                                                                                                                          aaaatttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGttaat           <  1:2147182/122‑1 (MQ=255)
                                                                                                                            aattttattttttttttttttttATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAATTATATAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGttaatta         <  2:2326528/121‑1 (MQ=255)
                                                                                                                            aaatagcttttttttttttttttATAAATGAAATATAAATTTTAGGTATTTTTTTTTATAATTATAATAAAAAATATAAAGGAAATATTATTGTTAAAATTAAT‑AAATAAATTTTTTAATTttaatta         >  2:1648122/8‑128 (MQ=255)
                                                                                                                            aaaatttttttttttttttttttATAATTGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGttaatta         <  2:1743969/124‑1 (MQ=255)
                                                                                                                               agctttttttttttttttttATAATGTAAATAAAAATTTTAGTTATTTTGTTGTAAAAGTTTCTAAAAAAAAATAAAGGAAAAAAT‑TTTTTTAAATTAATAAATAAAATTATTTAATTTTTATTaaaa      >  1:2681741/4‑128 (MQ=255)
                                                                                                                                 atttttttttttttttttATAATGGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGTTAATTAAAACt    <  2:1917043/127‑1 (MQ=255)
                                                                                                                                   ttttttttttttttttATAATGGAATTATTATTTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGTTAATTAAAACTta  <  1:473395/128‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                
ATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TA‑TTTTTTGTATATGCAAA‑TTTTAAAAGAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT‑ACTAAAATTATTTAATGTTAATTAAAACTTA  >  CP016816/165126‑165380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑