Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4890A.49.0% 486.1 / inf 473intergenic (+267/‑408)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (139/102);  new base . (32/200);  total (171/302)
Fisher's exact test for biased strand distribution p-value = 4.75e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-03

Only 100 of 501 total aligned reads displayed.

ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAATATATATCTAATATATATAATACTATA  >  CP016816/202370‑202616
                                                                                                                           |                                                                                                                                  
ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCAC‑‑Ttaaaaattaaaa                                                                                                                          >  1:1581529/1‑128 (MQ=255)
ataataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAAAAAACCCAtt‑‑‑aaaaataaaaa                                                                                                                         >  1:2384500/1‑128 (MQ=255)
 taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTT‑ATCTTAAC‑AAAT‑AAAAAAAAAACAATAACC‑‑ttaaaaattaaaaa                                                                                                                         >  2:3692022/1‑128 (MQ=255)
   ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAATAAAAAAAAAAACAAACACC‑‑Ataaaaaataaaaaa                                                                                                                        >  1:979832/1‑128 (MQ=255)
    aaaacttaattttaattaaAATAAAATAAAATTAATAATTA‑TAAAAATTTATATCCTCGTTATATATTTTTTTATTTTTTT‑ATATTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaa                                                                                                                      <  2:729832/117‑1 (MQ=255)
     atttttAAAGTTAAATTAAAAAAAATAAAATTTATAATTC‑TTATAATTTTTCACCTGTTTTTTTTTTTTTTTTTTTTTTT‑TTTTTCAC‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaa                                                                                                                     <  1:3610623/124‑1 (MQ=255)
     agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTAATTTTTTATTTTTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaa                                                                                                                     >  1:353619/1‑128 (MQ=255)
       atttAAGGTTAATTAAAATACAATAAAATTAAAAAATA‑TTAAAAATAATAACCTCGTGTACTATTTTTTTATTTTTTT‑ATAATCAC‑AAAA‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaa                                                                                                                   <  2:2085019/126‑1 (MQ=255)
        gTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAAAAATCCAC‑‑‑TAAAAAATtaaaaaaaaaaaa                                                                                                                 >  1:1516758/1‑128 (MQ=255)
         ttaattttaattaaAATAACAAAAACTTAAAAATAT‑TTTTAAATTTTATCCACGTTATATATTTTTTTATTTGTTT‑ATCTTCAA‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaa                                                                                                                 <  2:3492774/122‑1 (MQ=255)
         ttAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTATTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAAACCC‑‑‑ATAAAAAATaaaaaaaaaaaaaa                                                                                                                >  1:4281435/1‑128 (MQ=255)
          tAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATAACC‑‑TTAAAAAATaaaaaaaaaaaaaa                                                                                                                >  2:213672/1‑128 (MQ=255)
          tAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaa                                                                                                                >  2:4445037/1‑128 (MQ=255)
          tAAGGTTAATTAAAATACCATAAACATAATAATTA‑TTAAAATGTAGATCCTCGAGATCTATATTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaata                                                                                                                >  1:754399/1‑126 (MQ=255)
          tAAGGTTAATTAAAATACAATAAAATTAATAATTA‑TTATAATGTATATCATCTATATCTATTTTTATATATGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATAAC‑‑ATTAAAAATTaaaaaaaaaaaaaa                                                                                                                >  2:2810787/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                               >  2:3638807/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCC‑‑‑AATAAAAAATtaaaaaaaaaaaaaaa                                                                                                              >  1:1387722/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCAAC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                               >  2:1898792/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaa                                                                                                               >  2:4252759/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTA‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCACC‑‑ATAAAAAATaaaaaaaaaaaaaaa                                                                                                               >  2:2986540/1‑128 (MQ=255)
           aaGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTCATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAAATaaaaaaaaaaaaaaa                                                                                                               >  1:468665/1‑128 (MQ=255)
            aGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                              >  2:3651072/1‑128 (MQ=255)
            aGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                              >  1:2322825/1‑128 (MQ=255)
            aGGTTAATTAAAAAACCATAAAATTAATAATTA‑TAATAATGTATATACTCGTGATCTATTTTTTTAATTTTTT‑ATATTCAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaa                                                                                                              <  2:328096/128‑1 (MQ=255)
             ggTTAATTAAACTACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTCTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑‑TAAAAAATtaaaaaaaaaaaaaaaaa                                                                                                            >  2:3938873/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                             >  2:3532233/1‑128 (MQ=255)
             ggTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaa                                                                                                             >  1:4303933/1‑128 (MQ=255)
               ttaattaaAAAACAATAAAATTAAATATTT‑TTTTAATTTATATACTTTTTTTTATTTTTTTTATTTTTTT‑ATTTTAAA‑AAAT‑AAAAAAAAAAAAAACCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaac                                                                                                           <  1:3797956/128‑2 (MQ=255)
                taattaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                                          >  2:3890043/1‑128 (MQ=255)
                taattaaAATACCATAAAAATATAAATTA‑TTATAAAGTTTTACCTAGGGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAA‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaac                                                                                                          <  1:3557710/128‑2 (MQ=255)
                 aattaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                                         >  1:3771777/1‑128 (MQ=255)
                 aattaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTATATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaata                                                                                                         >  2:1533141/1‑126 (MQ=255)
                  attaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCACC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                                        >  1:1302124/1‑127 (MQ=255)
                  attaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATATGTTT‑ATCTAAAC‑AAAT‑AAAAAAAAAACAAACAACTTAAAAATAaaaaaaaaaaaaaaaaaaaaaa                                                                                                          >  1:2853383/1‑128 (MQ=255)
                  attaaAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTAATTTATTATTTGTAT‑ATCTTCAC‑AAAT‑AAAAAAAAAAAAATCCAC‑‑AAAAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                                        >  2:2398110/1‑127 (MQ=255)
                   ttaaAATACCATAAAATTAATAATTA‑ATAAAATTTATATACAAGTTATCTATTTTTTTATTTTTTT‑ATATTCAA‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaactaa                                                                                                       <  2:1229369/128‑5 (MQ=255)
                     aaaaTACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  1:2980086/1‑123 (MQ=255)
                     aaaaTACCATAAACTTAATAATTC‑TAATAATGTAGATACTCGTTATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAAAACCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaacaaaaa                                                                                                     >  1:1946110/1‑122 (MQ=255)
                      aaaTACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                    >  1:4212074/1‑123 (MQ=255)
                       aaTACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                   >  1:3866567/1‑128 (MQ=255)
                       aaTAACATAAAAATAATAATTA‑TTATAAATTATATACTCGTTATCTATTTTTTTTTTTTTTT‑ATATTAAA‑AAAA‑AAAAAAAAAAAAAAACCC‑‑TTAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTaaaaa                                                                                                  <  2:3759612/128‑1 (MQ=255)
                        aTAACATAAAATTAATAATTA‑TTATAATTTATATACTTTTGATATATTTTTTTTTTTGTTT‑TTTTTAAA‑AAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                                 <  1:3398008/128‑1 (MQ=255)
                          aacATAAAATTAATAATTA‑TTATAATTTATATAATCGTTATTTATTTTTTTATTTTTTT‑ATCTTAAA‑AAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                              <  2:1594418/126‑1 (MQ=255)
                          aaaaTAAAATTAATAATTA‑TTATAATTTATAACCTAGTTATCTATTTTTTTTTTTTTTT‑ATATTAAA‑AAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAaaacaaa                                                                                              <  2:4161195/125‑1 (MQ=255)
                            taaaaaataaaaaatTA‑TTAAAATTTATTTCCTAGTTATCTATTTTTTTATTTTTTT‑AAATTCAC‑AAAT‑AAAAAAAAAAAAATAACC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAAct                                                                                            <  2:523251/116‑1 (MQ=255)
                             aTAAAATTAATAATTA‑ATATAATTTATAACCACTTGATAAATTTTTTTATTTTTTT‑ATAATAAA‑AAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                          <  2:4338561/128‑1 (MQ=255)
                               aaaaataataaTTA‑TTAAAATTTATATAATAGTTTTATATTTTTTTATTTTTTT‑ATATTAAA‑AAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                         <  2:3668872/123‑1 (MQ=255)
                               aaaCTTAATAATTA‑TTATATTGTATTTACTTGTTTTATATTTTTTTATTTTTTT‑AACTTAAA‑AAAT‑AAAAAAAAAAAAAACCCC‑‑TTAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                                          <  1:3934040/128‑1 (MQ=255)
                               aaaCTTAATAATTA‑TTAAAATGTATATACTAGTGATCTATTTTTTTATTTTTTT‑ATATTAAC‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                         <  2:3453706/128‑1 (MQ=255)
                                aaattAAAAATTA‑TTATAATGTATATACTCGTTATATATTTTTTTATTTTTTT‑ATTTTAAA‑AAAT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                                         <  2:1670437/125‑1 (MQ=255)
                                 aattAATAATTA‑TTATAAATTATATACTTTTTAATTATTTTTTTTTTTTTTT‑ATATTAAA‑AAAT‑AAAAAAAAAACAATGCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                                      <  2:3557814/126‑1 (MQ=255)
                                    taataaATCTTTAAAATG‑AGATACCCCTGTTCTATTTTTTTTTTTTTTA‑ATAAACACAAAAA‑AAAAAAAAAACAAACCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAtt                                                                                       <  2:4201006/128‑1 (MQ=255)
                                         atTC‑TTATAATTTATAAAATCTTTATTTTTTTTTTTATTTTTTT‑ATCTTAAA‑AAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑TTAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAt                                                                              <  2:575911/128‑1 (MQ=255)
                                                 taATTTAGATCCTCGTGATATATTTTTTTATTTTTTT‑ATCTTCAA‑AAAT‑AAAAAAAAAACAATACCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtat                                                                       <  2:4572629/128‑1 (MQ=255)
                                                  aaTGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑TAAAAAACAACTATATTTGATAAAAATGCCttt                                                                       >  1:1167506/1‑126 (MQ=255)
                                                    tttAGATCATTGTTAACTATTTTTTTTTTTTTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt‑at                                                                   <  2:177831/126‑1 (MQ=255)
                                                    attttataatCGTTATTTTTTTTTTTATTTTTTT‑ATTTTTAA‑AAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt‑a                                                                    <  1:4540869/119‑1 (MQ=255)
                                                    attatctCCTCGTGATCTATTTTTTTTTTTTTTT‑ATCTTAAC‑AAAT‑AAAAAAAAAACAAACCCC‑‑ATAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt‑a                                                                    <  2:4489606/122‑1 (MQ=255)
                                                       tgataatttttttCTTTTTTTATATTTTTTT‑AATTTAAA‑AAAA‑AAAAAAAAAACAATCCCATTTTAAATTA‑AAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt‑a                                                                    <  2:3069635/116‑1 (MQ=255)
                                                       tcttCCTCGTGATGTTTTTTTTTTTTTGTTT‑ATATTCAC‑AAAA‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACca                                                               <  1:4432224/125‑1 (MQ=255)
                                                       aGATCCTAGTTATATATTTTTTTATTTTTTT‑ATCATCAA‑AAAT‑AAAAAAAAAAAAAACCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACca                                                               <  2:3719525/128‑1 (MQ=255)
                                                         ataatttttatttatttttttattttttT‑TTTTTAAA‑AAAT‑AAAAAAAAAAAAATAACATT‑‑‑AAAAAT‑‑TTAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACcac                                                              <  1:2116515/116‑1 (MQ=255)
                                                           tatagtttttatttttttttttttttT‑ATATTAAA‑AAAA‑AAAAAAAAAAAAATACACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACcac                                                              <  1:1012030/116‑1 (MQ=255)
                                                           attctttttttatttttttattttttT‑ATATTAAA‑AAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACcaca                                                             <  1:3721246/118‑1 (MQ=255)
                                                              cGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAACAATATTTATTAAAAATCACTATT‑AAAcccaatata                                                         >  1:4056372/1‑121 (MQ=255)
                                                                 tttaaaattttttAATTTTTT‑ATATTAAA‑AAAA‑AAAAAAAAAAAAATCAAC‑‑TTAAAAATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAAATCCCTATT‑ATACCACatttt                                                         <  2:2746129/121‑1 (MQ=255)
                                                                  ttttttttttttattttttt‑aTAT‑‑AA‑AAAA‑AAAAAAAAAAAAATACCCTTT‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                    <  1:2362556/123‑1 (MQ=255)
                                                                  ttCTATTTTTTTATTTTTTT‑ATATTTAA‑AAAA‑AAAAAAAAAAAAAACAACTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatt                                                      <  2:2889747/127‑1 (MQ=255)
                                                                  atatttttttttATTTTTAT‑AAATTAAA‑AAAA‑AAAAAAAAAAAAATCCC‑‑‑ATAAAAAAT‑‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                    <  2:1292978/123‑1 (MQ=255)
                                                                  atatATTTTTTAAATTTTTT‑ATATTAAA‑AAAT‑AAAAAAAAAAAAATTCCATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttattt                                                     <  2:865694/125‑1 (MQ=255)
                                                                    aattttttttattttttt‑aTATTAAA‑AAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACattttatttt                                                    <  1:1149412/125‑1 (MQ=255)
                                                                     taatttattATTTTTTT‑ATAATATA‑AAAT‑AAAAAAAAAACAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTAttttt                                                   <  2:3360331/125‑1 (MQ=255)
                                                                      tattttttATTTGTTT‑ATATTAAA‑AAAA‑AAAAAAAAAAAAAATCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTa                                                  <  1:1094247/126‑1 (MQ=255)
                                                                      aattttttATTTTTTTAATTTTAAA‑AAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTAttttt                                                   <  1:1663211/126‑1 (MQ=255)
                                                                      aTTTTTTTAATTTTTT‑ATATTCAA‑AAAT‑AAAAAAAAAAAAATAACCAT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTa                                                  <  2:2213937/128‑1 (MQ=255)
                                                                       tttttttattttttt‑aTCTTAAA‑AAAT‑AAAAAAAAAAAAAACCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTaa                                                 <  2:1132564/128‑1 (MQ=255)
                                                                        ttttttttttttTA‑TTATTAAA‑AAAT‑AAAAAAAAAAATATCACCTT‑‑‑AAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTaaa                                                <  1:3695390/128‑1 (MQ=255)
                                                                        ttttttATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑TAAAAAAAAAATTAATTTATT‑AAATTACAAATTAAAACAAAATTTTATTTTTaa                                                 >  1:3992599/1‑128 (MQ=255)
                                                                        ttttttATT‑‑TTT‑ATATTAAA‑AAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                             <  1:2441229/128‑1 (MQ=255)
                                                                           ttttttTGTTT‑ATATTCAA‑AAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                             <  1:3006469/128‑1 (MQ=255)
                                                                           tttATTTTTTT‑TTTTTAAC‑AAAA‑AAAAAAAAAAAAATCCCATT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATa                                             <  1:4401669/128‑1 (MQ=255)
                                                                            ttATTTTTTT‑ATCTTAAC‑AAAT‑AAAAAAAAAAAAATCACCTT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAg                                           <  2:4073829/128‑1 (MQ=255)
                                                                            ttATTTTTTT‑AAATTAAA‑AAAT‑AAAAAAAAAAAAAT‑‑AAAATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAAt                                              <  2:4019490/128‑1 (MQ=255)
                                                                            atATTTTTTT‑ATAATAAA‑AAAA‑AAAAAAAAAACAATACCATT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAAATCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATaa                                            <  2:4528116/127‑1 (MQ=255)
                                                                              aTTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAACTAAATTTATAAAAATTCCCTATT‑AAACCAATTTTTTTTTTTAAAAAAAAat                                         >  1:3424900/1‑128 (MQ=255)
                                                                                   ttt‑ATCTTAAC‑AAAT‑AAAAAAAAAAAAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑g                                   <  2:3402555/128‑1 (MQ=255)
                                                                                   ttt‑ATATTCAC‑AAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑‑‑TAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACa                                <  2:1422018/128‑1 (MQ=255)
                                                                                    tt‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACAAAAAACAAACTCAATTTATTAAAATAACCTATT‑ATCACACAATTAATTATTAAAAAAATATAttaa                                    >  1:4171638/1‑126 (MQ=255)
                                                                                    tt‑ATATTTAA‑AAAA‑AAAAAAAAAACAATACCCTT‑‑‑‑AAAAT‑TTAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GAc                                 <  2:230556/128‑1 (MQ=255)
                                                                                       taatttaaaaaaT‑AAAAAAAAAACAATCCAATT‑‑‑AAAAAT‑‑TAAAAAAAAAAAAAAAAAACAAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACa                                <  1:3507974/119‑1 (MQ=255)
                                                                                       aaatatac‑aAAA‑AAAAAAAAAAAAAAACCC‑‑ATAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GAc                                 <  2:2122535/122‑1 (MQ=255)
                                                                                         aataac‑aAAT‑AAAAAAAAAAAAATCACCTT‑‑‑AAAAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACata                              <  2:2465492/124‑1 (MQ=255)
                                                                                           ataaaaaaa‑aaaaaaaaaaCAATCCCCTT‑‑‑AAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACataa                             <  2:3974733/123‑1 (MQ=255)
                                                                                                aaaT‑AAAAAAAAAAAAATACCATT‑‑‑AAAAATT‑‑AAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATAACACATTTTATTTTTAAAATAAGATATT‑‑GACATAatatata                       <  1:17349/128‑1 (MQ=255)
                                                                                                  aT‑AAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAatatata                       <  2:4126298/128‑1 (MQ=255)
                                                                                                   aaaaaaaaaaaaaaaaACCC‑‑TTAAAAAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAatatata                       <  2:1057692/126‑1 (MQ=255)
                                                                                                   aaaaaaaaaaaaaaaaACCC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAatatatat                      <  2:3821220/126‑1 (MQ=255)
                                                                                                     agaaaaaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAAATCAATTTATTAAAATTAAATATT‑AAACCAATATATATTTTTAAAATAAGATATTTAAAAATAatatatat                      >  2:4392601/3‑128 (MQ=255)
                                                                                                          aaaaaCAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAA‑‑AAAAACAAAAACTATTAATAAAAAAAACAATTT‑ATAAAACATTTTTTTTTTAAAATAATATATA‑‑GACAAAATAAATATATaaatt               >  1:4097246/1‑125 (MQ=255)
                                                                                                                        cttaaaaattaaaaaAAAAAAAAAAATAAAACTAATAATAAACTCTATTTCTTAAAATTACCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAATATATATCTAATATATATAATACt     <  2:2575707/127‑1 (MQ=255)
                                                                                                                           |                                                                                                                                  
ATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTC‑TTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTT‑ATCTTCAC‑AAAT‑AAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATT‑ATACCACATTTTATTTTTAAAATAAGATATT‑‑GACATAATATATATCTAATATATATAATACTATA  >  CP016816/202370‑202616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑