BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.8%	1939.3 / NA	615	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (341/272); total (342/273)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	99.4%	1894.3 / NA	615	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (0/4); new base T (342/269); total (342/273)
Fisher's exact test for biased strand distribution p-value = 3.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 644 total aligned reads displayed.

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTAAACGT  >  CP016816/420035‑420280

||

 taattCAGTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                           <  2:1798446/127‑2 (MQ=255)

    ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                        <  2:716273/128‑5 (MQ=255)

     tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                       <  1:421139/128‑6 (MQ=255)

      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                      <  1:1129299/128‑1 (MQ=255)

       attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                        <  2:1927513/125‑5 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    <  1:660964/128‑1 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    <  1:847900/128‑1 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTATTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  2:737046/1‑128 (MQ=255)

        ttcatACTTTTAGTCTAATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    <  2:1590677/128‑1 (MQ=255)

           atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 >  2:858307/1‑128 (MQ=255)

           atAATTTTAGACTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 <  2:435832/128‑1 (MQ=255)

            tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                <  2:1763329/128‑1 (MQ=255)

             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                               >  1:1111101/1‑128 (MQ=255)

              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  1:151278/128‑1 (MQ=255)

              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  1:1569568/128‑1 (MQ=255)

              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATACATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  2:76453/128‑1 (MQ=255)

               ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                             <  2:1669692/128‑1 (MQ=255)

               ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                             <  2:1458865/128‑1 (MQ=255)

               ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                             <  1:599435/128‑1 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           >  1:791314/1‑128 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           <  2:838800/128‑1 (MQ=255)

                  tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                          <  2:1706575/128‑1 (MQ=255)

                   aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAATTTTAATTTTAATAACTCAAATAtttt                                                                                                         >  1:1279182/1‑128 (MQ=255)

                    gTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                        <  2:1725977/128‑1 (MQ=255)

                      ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCa                                                                                                      >  2:924763/1‑128 (MQ=255)

                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  2:534350/128‑1 (MQ=255)

                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  1:389976/128‑1 (MQ=255)

                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     >  1:1776374/1‑128 (MQ=255)

                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  2:472070/1‑128 (MQ=255)

                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                               >  2:1898747/1‑128 (MQ=255)

                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                               >  1:1320197/1‑128 (MQ=255)

                               cTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATTCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                             <  1:92795/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  2:453132/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  1:267547/128‑1 (MQ=255)

                                   gTTTCTAAAATTATTTTATGATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         >  2:133598/1‑128 (MQ=255)

                                    tttCTAAAATTATTTTATCATTTAAATAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                        <  2:263266/128‑1 (MQ=255)

                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:1215795/1‑128 (MQ=255)

                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTGTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:1010542/1‑128 (MQ=255)

                                      tatataaTTATTTTATAATTTAAAAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      <  1:509648/123‑1 (MQ=255)

                                      tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      >  2:514475/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:594954/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:1508060/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:1172611/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:1318943/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  1:1750106/1‑128 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  2:1013624/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    >  2:675587/1‑128 (MQ=255)

                                           aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                 <  1:997522/128‑1 (MQ=255)

                                                 tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                           >  2:1258075/1‑128 (MQ=255)

                                                  ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          >  2:344649/1‑128 (MQ=255)

                                                   tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                         <  1:337511/128‑1 (MQ=255)

                                                     tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                           >  1:1882269/1‑124 (MQ=255)

                                                      cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                      >  2:232466/1‑128 (MQ=255)

                                                      cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                      >  1:728475/1‑128 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                    >  1:550193/1‑128 (MQ=255)

                                                         ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                   >  1:469727/1‑128 (MQ=255)

                                                               aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑tt                                                           <  2:10964/128‑1 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑ttt                                                          >  1:1541909/1‑128 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑ttt                                                          <  2:797817/128‑1 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑ttt                                                          <  1:1907723/128‑1 (MQ=255)

                                                                 ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑tttt                                                         >  2:1556803/1‑128 (MQ=255)

                                                                  taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTa                                                        >  1:1414611/1‑128 (MQ=255)

                                                                  taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTa                                                        >  1:203763/1‑128 (MQ=255)

                                                                   aataCTTTTATTAGTTGATATATTATAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTAt                                                       <  2:1077973/128‑1 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATTCt                                                    >  2:1840595/1‑128 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑tttt                                                         <  1:1666417/123‑1 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCt                                                    >  1:1684954/1‑128 (MQ=255)

                                                                       cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTAAGCTc                                                   >  2:414887/1‑128 (MQ=255)

                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCg                                                  >  1:1479393/1‑128 (MQ=255)

                                                                          ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGATCt‑tt                                               >  2:390527/1‑128 (MQ=255)

                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGATCt‑ttt                                              >  2:564767/1‑128 (MQ=255)

                                                                            attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑tttt                                             >  1:1629631/1‑128 (MQ=255)

                                                                                   gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑ttttttttttt                                      >  1:1926407/1‑128 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑tttttttttttttt                                   >  2:1970816/1‑128 (MQ=255)

                                                                                       tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑ttttttttttttttt                                  >  2:1873394/1‑128 (MQ=255)

                                                                                         ttAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑ttttttttttttttttt                                >  1:195542/1‑128 (MQ=255)

                                                                                          tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑tttttttttttttttttt                               >  1:1706884/1‑127 (MQ=255)

                                                                                            taaaaATAATATCAATTTTTTAAAAAATTTTTAATTTTAATAATTTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATCTATTAATTAA‑‑TTTTTTGCTCG‑ttttttttttttttttacaa                             <  1:1080944/127‑5 (MQ=255)

                                                                                               aaataatataaattttttAAAAAATTTTTAATTTTTATAAATTTATTATTTTTCAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAA‑‑TTTTATTCTTGTTTTTTTTTTTTTTTTTT‑ACaaa                          <  1:124176/116‑1 (MQ=255)

                                                                                               aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCGTTTTTTTTTTTTTTTTTT‑ACaaa                          >  2:1039922/1‑128 (MQ=255)

                                                                                               aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAAAAACTTTATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAA‑‑TTTTATGCTCGTTTTTTTTTTTTTTTTTT‑ACaaa                          <  2:1630064/128‑1 (MQ=255)

                                                                                                 ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑ACAAAAat                       <  2:1790672/128‑1 (MQ=255)

                                                                                                   aataGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTTTTAAATTTTTTTAAAATCATTATCTATTAATAAA‑‑TTTTATGCTCG‑‑‑TTTTTTTTTTTTTTT‑ACAAAAATATaa                   <  2:664058/126‑1 (MQ=255)

                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑AAAATAATAAAatt                 >  2:1246361/1‑128 (MQ=255)

                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTAG‑TTTTTTTTTTTTTTTTT‑ACAAAAAAATAatt                 >  2:970723/1‑128 (MQ=255)

                                                                                                        cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCGTTTTTTTTTTTTTTTTTT‑AAAAAAAAATAatt                 >  2:1410107/1‑128 (MQ=255)

                                                                                                          cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑AAAAAAATATAatttat              >  2:111442/1‑128 (MQ=255)

                                                                                                          cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTT‑ACAAAAATATAatttatt             >  1:1369895/1‑128 (MQ=255)

                                                                                                          attttttATAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTAAATTATTTTTAAATAATTATATATTAATTAA‑‑TTTTATTATC‑‑TTTTTTTTTTTTTTTTT‑ACAAAAATATAatttatt             <  2:320000/127‑1 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑ACAAAAATATAatttatt             >  2:629527/1‑128 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑‑ATTTTTTTTTTTTTTA‑AAAAAAATAAAattaattt            >  2:1137981/1‑128 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑‑‑‑‑TTTTTTTTTTTTT‑ACAAAAATATAAtttattttat         >  1:1024573/1‑128 (MQ=255)

                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGATCG‑‑TTTTTTTTTTTTTTTT‑ACAAAAATATAattaattt            >  1:1649866/1‑128 (MQ=255)

                                                                                                            tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTTAAAAAAAAAAAAatttatt             >  1:482692/1‑128 (MQ=255)

                                                                                                            tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑AAAAAAATATAatttattt            >  1:180427/1‑128 (MQ=255)

                                                                                                               ttATAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATTATTTTATATTAATAAATTTTTTTTTGTTT‑TTTTTTTTTTTTTTTTT‑ACAAAAATATAATTTATTTg           <  1:233814/128‑1 (MQ=255)

                                                                                                                 aaaaaaTTTTTAATTTTAATAAATGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTATATATTAATCAA‑‑TTTTATTCTCG‑‑‑TTTTTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTaaa     <  2:1655775/128‑1 (MQ=255)

                                                                                                                  taaaaTTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTAAAATAATTATATATTAATAAA‑‑TTTTATGCTTG‑‑TTTTTTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTaaa     <  1:441866/127‑1 (MQ=255)

                                                                                                                  aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑‑TTTTTTTTTTTTTTTT‑AAAAAAATATAATTTATTTTATaaaa     >  2:1376295/1‑128 (MQ=255)

                                                                                                                    aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCGTTTTTTTTTTTTTTTTTT‑AAAAAAAAATAATTTATTTTATTaaa     >  1:1851398/1‑128 (MQ=255)

||

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑TTTTATGCTCG‑TTTTTTTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTAAACGT  >  CP016816/420035‑420280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`