Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.8% 6907.0 / ‑4.2 2366intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (13/40);  new base T (1493/820);  total (1506/860)
Fisher's exact test for biased strand distribution p-value = 6.02e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2428 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑TAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347
                                                                                                                                |                                                                                                                                   
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTtaa                                                                                                                                   <  2:2220396/128‑1 (MQ=255)
 ttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTtaaa                                                                                                                                  <  2:473176/128‑1 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTtaaaa                                                                                                                                 >  1:5668866/1‑127 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTT‑TATGCAAA‑TTTTTAAAAaat                                                                                                                              >  2:5035665/1‑128 (MQ=255)
     tttAATTGAATAACTATACTAGTTTATAATATTAAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTATTAGATA‑TTTTTTGAA‑AATGCAAA‑TTTTTAAAAaat                                                                                                                              >  2:3760759/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAATAaattaat                                                                                                                          >  1:6294650/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAaattaat                                                                                                                          <  1:6736077/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                        <  2:2316273/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                     >  1:1650123/1‑128 (MQ=255)
                  ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                                 <  1:3394513/128‑1 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                              <  2:4855066/128‑1 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTtaaaa                                                                                                           >  2:5796830/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                           >  2:2900441/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttt                                                                                                  >  2:1696354/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATCAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttt                                                                                                 >  2:2001623/1‑128 (MQ=255)
                                  tatttattTTAATTAATTTATTGTACTTTTATTTATTATTAATAAAATAAAAATATTATATTTTTAGTTA‑TTTTTTTTA‑TATGAAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGC‑ttttt                                                                                                <  1:1533576/128‑1 (MQ=255)
                                  tatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttt                                                                                                >  1:25887/1‑128 (MQ=255)
                                     ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttt                                                                                             >  2:6814070/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttt                                                                                          >  1:273906/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttt                                                                                          >  1:4364836/1‑128 (MQ=255)
                                         tttCATTAATTTTTCCCAATTTTATTTAATTTTAATTAAATAAAAATATTATCTTTTTTTATA‑TTTTTTTTT‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑tttttttttttt                                                                                         <  2:3006915/128‑1 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGC‑tattttttacaagt                                                                                       <  2:6620593/128‑7 (MQ=255)
                                             atttatttTTAGCAATTTTATTTATTATTTATAAAATAAAAATTTTTTATTTTTATATA‑TTTTTTTTA‑TATGCAAA‑ATTTTTAAAAATTAATTTGAACTTGTAAAAAAAATC‑tttttttttttttttt                                                                                     <  2:594234/128‑1 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttttttttttt                                                                                     >  1:6082202/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATAATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAAATTGAACTTGTAAAAAATAGC‑tttttttttttttttt                                                                                     >  2:340903/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttt                                                                                    >  1:1344460/1‑128 (MQ=255)
                                                 aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑tatttttttttttttttaaa                                                                                 >  1:5000415/1‑125 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑tttttttttttttttattaaa                                                                                >  2:3510461/1‑125 (MQ=255)
                                                   ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAAATAATTTGAACTTGTAAACAATAGC‑tttatgttttttgtttttaaat                                                                               >  2:4482439/1‑124 (MQ=255)
                                                    tATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAt                                                                             >  1:5445311/1‑128 (MQ=255)
                                                     ttagttaatGTATTTATTTGTAACAAAATTAAAATATTAATTTATTTTAAA‑TTTTTTTTT‑TTTGGAAA‑TTTTTAAAAAATTTATTTTTAAATGTAAAAAAAA‑‑‑TGTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:6629263/120‑1 (MQ=255)
                                                     atttcaattGTATTAATTATTAATAAACAAAAAATAATATATTTTTATATA‑TTTTTTTTA‑TATTCAAA‑TTTTTTAAAAATTAATTTTTAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATg                                                                            <  2:4053006/121‑1 (MQ=255)
                                                      tggcaatttattGAATTTCTAAGAAACTAAAACTATTATTTTTTTACTTA‑TTTTTTTTT‑TATGCAAA‑TTTTTTAATAATTAATTTTAACTTGTATAAAATAGC‑‑‑GTTTTTTTTTTTTTTTTATAATGGa                                                                          <  1:315021/117‑1 (MQ=255)
                                                      tcccACTTTTTTTAATTATTTAGAAACTAAAAATTTTATTTTTTTATATT‑TTTTTTTTA‑TATTCAAA‑TTTTTAAAAAATTAAATTTAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:2462367/125‑1 (MQ=255)
                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGC‑ttttttttttttttttttaaaattaaat                                                                         >  1:2241061/1‑118 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAata                                                                      >  2:1403168/1‑128 (MQ=255)
                                                           attttattaattattaatAAACTAAAAATATTATATTTTTATATA‑TTTTTTTTA‑TATTCAAA‑TTTTTAAAAAATTAAATTGAACTTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                    <  1:3929934/124‑1 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTTATAAtttaaaata                                                                     >  1:1876306/1‑120 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTT‑‑TAATTGAAAtatat                                                                   >  1:6838813/1‑127 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATaa                                                                   >  2:6732300/1‑128 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTTTAAaaataaat                                                                 >  1:5191187/1‑128 (MQ=255)
                                                                    attttttataaaataaaaataTTTTTATTTTATATA‑TTTTTTTTA‑TTTTTAAA‑TTTTTTAAAAATTTATTTTTAATTTTTAAAAATATC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                              <  1:1143239/119‑1 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTT‑‑TAATGTAAAATTAAATTTTat                                                            >  1:5918667/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑TTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTat                                                            >  1:2361229/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTTAAAATTTAAAtttttgt                                                           >  2:1503451/1‑125 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTAAAATAAAAATTTTatt                                                           >  2:883239/1‑126 (MQ=255)
                                                                       attaataaACAAAAACTATTATATTTTTATTAA‑TTTTTTTTATTATCAAAA‑TTTTTAAA‑AATTAATTTTAAATTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  1:4509164/126‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAATTTTTAGTTa                                                         >  1:4699492/1‑128 (MQ=255)
                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAAattttttt                                                          >  2:4615774/1‑124 (MQ=255)
                                                                         taataaACTAAAAAATTTAT‑TTTTTAATTA‑TTTTTTTTT‑TTAGTAAATTTTTTAAAAAATTAATTTTAAATTTTAAAAAA‑ATG‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  1:2896075/128‑1 (MQ=255)
                                                                          aaGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTtatttatt                                                       >  1:4176547/1‑128 (MQ=255)
                                                                           aGAAAATAAAAATTTTATATTTTTATATA‑TTTTTTTTT‑TTTTTAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                      <  1:4598768/128‑1 (MQ=255)
                                                                            taaactaaaactaTTATTTTTTTTTTTA‑TTTTTTTTA‑TTTTCAAA‑TTTTTAAAAAAATAATTTGAACTTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                      <  1:6409723/127‑1 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTtcttgt                                                >  2:209568/1‑128 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttt                                                 >  1:6512890/1‑127 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tt                                                                                                   >  2:6186251/1‑78 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑‑TTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTGTATaat                                           >  2:3103870/1‑127 (MQ=255)
                                                                                   aaactaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTATTTATTttgtttta                                               >  2:5475991/1‑125 (MQ=255)
                                                                                    atattttttgtttttATATA‑TTTTTTGTT‑TATTCAAA‑TTTTTAATATATTAATTTGTACTTTTAAAAAATA‑‑‑TCATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                            <  1:5378731/118‑1 (MQ=255)
                                                                                    aattattttTTTTTTTTATA‑TTTTTTGTA‑TATTCAAA‑TTTTTAAAAAATTATATTTAAATTGTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  2:4342302/125‑1 (MQ=255)
                                                                                     actaTTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATaa                                            >  2:1067847/1‑128 (MQ=255)
                                                                                     aatattttTTTTATAGATATTTTTTTGAA‑TA‑GCTAA‑TTTTTAAAAATATTATTTGATATTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                            <  1:3621897/126‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATaatt                                          >  1:176240/1‑126 (MQ=255)
                                                                                       tattatCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTTATTATTGAAATATAAATTTTATGTttttttttttattatta                                         >  2:2408922/1‑119 (MQ=255)
                                                                                        attatCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGtttttttttttatatctga                                         >  1:3251221/1‑119 (MQ=255)
                                                                                        attatCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTGTGTTTTTTGTTGTATAATTAt                                        >  2:6066784/1‑128 (MQ=255)
                                                                                         ttatTTTTTTTTTTA‑TTTTTTGTA‑TTTTCAAA‑TTTTTAAAAAAATAATTTTTACTTGT‑‑AAAATAAT‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  2:6475008/128‑1 (MQ=255)
                                                                                         ttatTTTTTTATATA‑TTTTTTGTA‑TATTGAAA‑TTTTTAAAAAATTAATTTTTAATTTTTAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:3043652/128‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAAATTAAATTTTTGTTATTtttttgaaaaatttt                                        >  2:1854427/1‑119 (MQ=255)
                                                                                          tattattttAGTTA‑TTTTTTTTA‑TATTCAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  1:838175/123‑1 (MQ=255)
                                                                                          tatTTTTTTATTTA‑TTTTTTGTA‑TTTTCAAA‑TTTTTAAAAAATTAATTTTTACTTGTAAAAAATTGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                       <  2:5237418/128‑1 (MQ=255)
                                                                                          tatTTTTTTATATT‑TTTTTTTTA‑ATTTCAAA‑TTTTTAAAAAAATAAATTTTACATTTAAAAAAAAAC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                        <  1:5295266/128‑1 (MQ=255)
                                                                                           atttttttATATT‑TTTTTTTTT‑TTTTTTAA‑TTTTTAAATAATTTTTTTTTATTTTTAAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAATTATCt                                      <  1:935706/125‑1 (MQ=255)
                                                                                              tttttAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTATTTATTTTTTTTTATAATTaaaaaaa                                   >  1:3523525/1‑122 (MQ=255)
                                                                                               ttttAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTATGTTATTTTTTTTTATATTTATATaaa                                   >  1:6171930/1‑128 (MQ=255)
                                                                                               ttttAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TAATGAAAATATAAATTTTAGTTATTTTTTTTTATAAGttaataaaa                                  >  2:1228464/1‑120 (MQ=255)
                                                                                               ttttAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTAATTTTTTTTTATAATTATATaaaa                                  >  1:1001558/1‑128 (MQ=255)
                                                                                               ttttAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAAATAAAAATTAAAATTTTAGTTATATTTTTTAATAATTATATaaaa                                  >  1:3706966/1‑128 (MQ=255)
                                                                                                  ttttta‑tTTTTTTTT‑TTTTTAAA‑TTTTTTAAAAATTAAATTTAAATTTTTAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                               <  1:1997321/124‑1 (MQ=255)
                                                                                                   ttata‑tTTTTTTTT‑TATTAAAA‑TTTTTAAAAAATTATTTTTTAATTTTTAAAAATA‑‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑Ta                            <  2:513005/126‑1 (MQ=255)
                                                                                                     ata‑tTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATGGAAATAAAAATTTTAGTTATTTTGTTGTATAAGTTTATAAAAAAA‑Ta                            >  2:5678559/1‑128 (MQ=255)
                                                                                                      ta‑tTTTTTTTA‑TATTAAAA‑TTTTTAAAAAATTTATTTTAATTTTTAAAAAAT‑‑T‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑TAGaa                         <  2:2506476/128‑1 (MQ=255)
                                                                                                            tttGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAATTTGAAATTAAAATTTTAAGTATTTTTTTGTAAAATTATATAAAAAAA‑TAGAAAGGa                     >  2:5352289/1‑128 (MQ=255)
                                                                                                            tttGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATATATTTTATTTATTTTTTTTTCTAATTATATAAAAAAA‑TAGaaattaa                    >  1:4050536/1‑124 (MQ=255)
                                                                                                             tttta‑taTTTAAA‑TTTTTAAAAAATAAATTTTAAATTTTAAAAAATATG‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑TAGAAAGGa                     <  1:5558608/125‑1 (MQ=255)
                                                                                                               tttttattttaa‑TTTTTAAATAATTTATTTTTAATTTTTAAAAATTTT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑TAGAAAGGaa                    <  1:3821070/118‑1 (MQ=255)
                                                                                                               gTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTATTTATTTTTTTGTATAAGTAAATAAAAAAA‑TATAAATTaaaaaa                >  2:4194154/1‑128 (MQ=255)
                                                                                                                   tatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAATTTTATAAAAAAA‑TATAAAGTAAAAAatat             >  2:3928884/1‑128 (MQ=255)
                                                                                                                    atGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAT‑TATAAAGTaaaaaaat              >  1:1239471/1‑126 (MQ=255)
                                                                                                                     tGCAAA‑TTTTTAAAAAATTAATTTGCACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATGTAATTATAATTTTTATGATTTTTGTTGTATAAGTTTATATAAAAA‑TTGATAGGAAAAAat               >  1:5803686/1‑128 (MQ=255)
                                                                                                                     tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATTATGGAAATTTAAATTTTTTGTATTTTTTTTTGTAATTATATAAAAAAA‑TATAAAGTAAAAAattt             >  2:755657/1‑126 (MQ=255)
                                                                                                                     tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑TTTTTTTTTTTTTTTTTTTA‑AATTTAAATATTAATTTAATTTATTTATTTATATAA‑TATTAAAAAAAATTAAAATGGTAAAAattt             >  1:510712/1‑126 (MQ=255)
                                                                                                                      gCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTTTAAATATAATTTTAATTTATTTTTTTTTATAATTTTTTATAAATA‑TATAAATaaaaaattttt            >  1:4852504/1‑123 (MQ=255)
                                                                                                                      gCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAAAATAAATTTTAGGTATTTTTTTTTATAATTATAAAAAAAAA‑TAAAAAATAAAAAATAtt            >  2:3282925/1‑128 (MQ=255)
                                                                                                                       cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAAAAAGTAAAAATAATTTTTATTTATTTTTTTGTAAAAATTTATAAAAAAA‑AAAAAAGTAAAAAAAAtt            >  2:5556998/1‑128 (MQ=255)
                                                                                                                        aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATTAAATTATAATTTTTATGTATTTTTTTGTATATGTTTATAAAAAAA‑TATAAATAAAAAAAtttttta         >  1:2098785/1‑125 (MQ=255)
                                                                                                                         aa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATAAAAATA‑TAGTAATGAAAAAGTAttaaat        >  1:1429520/1‑124 (MQ=255)
                                                                                                                          a‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTTGTTATTTTTTTTTATAATTATAATAAAAAA‑AATAAAGTAAATAATAtttt          >  2:2687417/1‑126 (MQ=255)
                                                                                                                            ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATAAAAATTTTAGTTATTTTGTTTTCTAATTATCTAAAAAAA‑ATAATATGAATAAAAATTGTTaaa      >  2:6315570/1‑128 (MQ=255)
                                                                                                                            ttttAAAA‑AATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTAGTTTTTTTGTTTTATAATTATAAAAAAAAA‑AAAAAATAAAAAAATTTTATTaaaa     >  2:366237/1‑128 (MQ=255)
                                                                                                                                |                                                                                                                                   
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTA‑TATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAA‑TAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑