BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	6267.3 / NA	1966	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (4/5); new base T (1055/899); total (1059/907)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.6%	5988.9 / ‑3.2	1967	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (5/22); new base T (1054/886); total (1059/908)
Fisher's exact test for biased strand distribution p-value = 2.73e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2059 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

||

     aaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                                  >  2:1285321/1‑127 (MQ=255)

      aattaattCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttt                                                                                                                                 <  1:2024900/128‑3 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                                <  1:1343119/128‑4 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                                >  2:1851875/1‑125 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                                >  2:1495020/1‑125 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                                <  1:441349/128‑4 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                                <  1:4078600/128‑4 (MQ=255)

         tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                              <  1:3989971/128‑6 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          <  2:1963836/128‑1 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                          <  1:3034828/128‑1 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                        <  1:1116294/128‑1 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                        >  2:4035951/1‑128 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                        <  2:3098639/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  2:802077/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  2:491459/128‑1 (MQ=255)

                tACTTTTAGGATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  1:2757412/128‑1 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                      <  2:3231308/128‑1 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                     <  2:3513972/128‑1 (MQ=255)

                  cTTTTAGTATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                     <  2:3827383/128‑1 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                   <  2:5436523/128‑1 (MQ=255)

                    tttAGTCTAATAAATCTAAGTTTCTAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                   <  2:2426327/128‑1 (MQ=255)

                        gTCTCATATATCTATGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                               <  2:2996397/128‑1 (MQ=255)

                        gTCTCATAAATCTAAGGTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                               >  2:2103143/1‑128 (MQ=255)

                        gTCACATAAATATAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                               <  2:1720543/128‑1 (MQ=255)

                         tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAATTTTc                                                                                                              >  1:282277/1‑128 (MQ=255)

                         tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTATAATAACTGAATTATTTTTc                                                                                                              >  2:1400166/1‑128 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                             <  2:688544/128‑1 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  1:2235730/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  1:2561721/128‑1 (MQ=255)

                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                        >  1:4323343/1‑128 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                       >  2:4738305/1‑128 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAGAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     >  1:1689121/1‑128 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     >  1:4901805/1‑128 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     <  2:1601318/128‑1 (MQ=255)

                                    tAAGTTTCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   <  2:2640334/128‑1 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                   >  1:3543751/1‑125 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                                >  1:3408911/1‑128 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                                >  2:2778640/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  1:482600/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  2:4329169/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  2:4513429/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  1:603972/1‑128 (MQ=255)

                                           cTAAAATGATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACCGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  2:2867675/1‑128 (MQ=255)

                                            tAAAATTATTTTATCATTTAAAAAAATAATACTATTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:3721996/128‑1 (MQ=255)

                                            tAAAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:1000427/128‑1 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTAGAACTTTTATAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                         <  1:2668268/128‑1 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                         <  1:5012536/128‑1 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTAGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                         <  2:1123614/128‑1 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                        <  1:1892436/128‑1 (MQ=255)

                                                attattTTATCATTTAAAATAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGTACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  2:5508231/128‑1 (MQ=255)

                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                     >  1:5441782/1‑128 (MQ=255)

                                                   attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                    >  1:2934749/1‑128 (MQ=255)

                                                   attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                    <  1:3208521/128‑1 (MQ=255)

                                                     tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  <  1:985170/128‑1 (MQ=255)

                                                      ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                 >  1:4237125/1‑128 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  2:4999221/128‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               >  1:4640834/1‑128 (MQ=255)

                                                        aTCATTTAAAAAAATAATACATTTCTTAGATGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  2:5200355/128‑1 (MQ=255)

                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATAATTTTAAAATCATTATCTAt                                                                             >  2:4809380/1‑128 (MQ=255)

                                                               aaaaaaaTATTACTTTTATTAGTTGATATATTAGAAAACTACAAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                        <  2:526847/128‑1 (MQ=255)

                                                               aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                        >  2:3348027/1‑128 (MQ=255)

                                                                 aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                      <  1:3312872/128‑1 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                   <  2:2273997/128‑1 (MQ=255)

                                                                     ataataGATTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATAATTTTCAAATCATAATCTATTAATCAAtttt                                                                  <  1:2315732/128‑1 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                  >  1:238941/1‑128 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                  <  2:4238356/128‑1 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                  >  1:4733851/1‑128 (MQ=255)

                                                                     ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                  >  1:1786185/1‑128 (MQ=255)

                                                                        ataCATTTATTAGCTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAAATATTTTTAAAATTGTTAAATTAATTTCAAATCATTATGTATTAATCAATTTTA‑tt                                                              <  2:4672174/128‑2 (MQ=255)

                                                                           cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTc                                                           >  1:4474633/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttt                                                    >  2:4434731/1‑128 (MQ=255)

                                                                                  taGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             <  1:3616534/104‑1 (MQ=255)

                                                                                      tGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑tttttttttt                                             >  1:1600707/1‑128 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttttttttttttt‑t                                        >  2:5090956/1‑128 (MQ=255)

                                                                                           tattttaaaaATACTATCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTAAAATAATTATATATTAATCAAATTTA‑TGCTC‑G‑‑ttttttttttttt‑tt                                       <  1:1429147/122‑1 (MQ=255)

                                                                                           tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttttttttttttt‑tt                                       >  1:1202173/1‑128 (MQ=255)

                                                                                            atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttttttttttttt‑ttt                                      >  1:1951581/1‑128 (MQ=255)

                                                                                            atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TTCTC‑G‑‑ttttttttttttt‑ttt                                      >  1:3857642/1‑128 (MQ=255)

                                                                                            atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttttttttttttt‑ttt                                      >  1:2134918/1‑128 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCGTTATCTATTAATCAATTTTA‑TGCTC‑G‑‑ttttttttttttt‑tttttaaaa                                >  2:535883/1‑123 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTATCAAATCATTATCTATTAATCAATTATA‑TGCTC‑G‑‑TTTTTTTTTTTTTAATTT‑ACaa                                >  1:5324381/1‑128 (MQ=255)

                                                                                                   aaattattttaattttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAATTTTTTAAATTATTTTAAAATAATTTTATATTAATTAATTTTT‑TGTTG‑GT‑TTTTTTTTTTTTT‑TTTT‑ACaaa                               <  2:2526381/116‑1 (MQ=255)

                                                                                                   aaatactaGCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTCAAATCATTATATATTAATCAATTTTA‑TGCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑ACaaa                               <  1:3103181/125‑1 (MQ=255)

                                                                                                     ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTT‑AAAAAAat                            >  2:4160073/1‑128 (MQ=255)

                                                                                                     ataatttcaattttttAAAAAAATTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTTAAATTTTTTAAAATAATTATAAATTAATAAATTTTA‑TGCTCTT‑‑TTTTTTTTTTTTT‑TTTT‑ACaaaaa                             <  1:869926/118‑1 (MQ=255)

                                                                                                     aaaattacaattttttTAAAAATTTATAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTA‑TGCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑ACaaaaa                             <  1:2476767/118‑1 (MQ=255)

                                                                                                      tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTT‑AAAAAAatt                           >  1:5087861/1‑127 (MQ=255)

                                                                                                         ttgtttagttttTAAAATTTTTTAATTTTAATAAATGTATTTTTTTTTAAATTTTTTTATTTTTTTTAAATATTTATCTTTTTATCAATTTTA‑TTGTG‑G‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATaa                        <  1:5167539/120‑1 (MQ=255)

                                                                                                          ttaaCTTTTATAAAAAATTTTTTATTTTAAAAACTGAATTATTTTTAAAATTTTTAAATTATTATAAAATAATTATCAATTAATAAATTTTT‑TGCTCGG‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATaa                        <  2:3633862/125‑1 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑AAAAAAATATaa                        >  1:4550336/1‑128 (MQ=255)

                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATAAATCAATTTTA‑TGCTC‑GTTTTTTTTTTTTTTT‑TTTT‑ACAAAAATATaa                        >  1:4164832/1‑128 (MQ=255)

                                                                                                            cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAatt                      >  1:77293/1‑128 (MQ=255)

                                                                                                              attttttTAAAAATTTTTTATTTTTATAAATTAATTATTTTTTAATTTTTTAAATTTTTTTATAATTATTTTATTTTAATTAATTTTATTTTTT‑T‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAattta                    <  1:52562/127‑1 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAAAAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTAAAATAATTAAATATTAATAAATTTTA‑TTCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAatttat                   <  1:680893/128‑1 (MQ=255)

                                                                                                               ttttttAAAAAAATTTTAATTTTAAAAACTTTATTATTTTTCAAATTTTTAAATTATTTTAAAATAATTAAATATTAATAAATTTTA‑TTCTCGG‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAatttat                   <  1:5511964/128‑1 (MQ=255)

                                                                                                                 ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑GTTTTTTTTTTTTTTT‑TTTT‑AAAAAAAAAAAatttatt                  >  2:4595887/1‑128 (MQ=255)

                                                                                                                    tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTT‑AAAAAAATAAAATttattttatt             >  1:1500413/1‑128 (MQ=255)

                                                                                                                       aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑GT‑TTTTTTTTTTTTT‑TTTT‑AAAAAAATATATTTTATTTTATTaa           >  1:1276398/1‑128 (MQ=255)

                                                                                                                        taaTTTTTAATTTTAATAACTGTATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAAATAATTTTA‑TTCTC‑G‑‑‑TTTTTTTTTTTT‑TTTT‑ACAAAAATATAATTTATTTGATTAAACg        <  1:347260/127‑1 (MQ=255)

                                                                                                                         aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTTAAAAAAAATAAATATTATTTTATTAAAc         >  2:595063/1‑128 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑TGCTC‑G‑‑TTTTTTTTTTTTT‑TTTT‑ACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`