BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.4%	3891.2 / NA	1245	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (5/3); new base T (671/561); total (677/568)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.3%	3495.9 / NA	1242	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/19); new base T (673/548); total (675/567)
Fisher's exact test for biased strand distribution p-value = 2.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1248 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420041‑420281

||

cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                      <  1:2540259/128‑1 (MQ=255)

cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTCTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                      <  2:180623/128‑1 (MQ=255)

  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  1:336746/1‑128 (MQ=255)

  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  2:878955/1‑128 (MQ=255)

  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  2:3703711/1‑128 (MQ=255)

   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                   >  1:2509584/1‑128 (MQ=255)

      tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                <  1:3246478/128‑1 (MQ=255)

        cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  2:1393864/128‑1 (MQ=255)

        cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  2:433767/128‑1 (MQ=255)

           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           <  2:2465575/128‑1 (MQ=255)

           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATCAattatt                                                                                                           >  1:1005681/1‑128 (MQ=255)

            tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                          <  2:2611652/128‑1 (MQ=255)

             attctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                         <  2:4004348/126‑1 (MQ=255)

             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                         >  1:830771/1‑128 (MQ=255)

                atcATAAATCTAAGTTTCTAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                      <  2:1573512/127‑1 (MQ=255)

                 tcATAAATCTAAGTTTCTGAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  1:3756363/128‑1 (MQ=255)

                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  2:1100114/128‑1 (MQ=255)

                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  2:2992623/1‑128 (MQ=255)

                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  1:3579559/128‑1 (MQ=255)

                     aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                 >  1:606662/1‑128 (MQ=255)

                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                >  2:2914934/1‑128 (MQ=255)

                       atatAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                               <  2:1689652/125‑1 (MQ=255)

                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                               <  1:1757108/128‑1 (MQ=255)

                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                              <  1:2410642/128‑1 (MQ=255)

                        tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                              <  1:401365/128‑1 (MQ=255)

                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  1:1169115/128‑1 (MQ=255)

                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  1:1114367/128‑1 (MQ=255)

                             gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         >  2:3832386/1‑128 (MQ=255)

                              tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                        <  2:643707/128‑1 (MQ=255)

                              tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                        <  1:1432706/128‑1 (MQ=255)

                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                <  2:578256/119‑1 (MQ=255)

                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  2:2236070/1‑128 (MQ=255)

                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:3523177/1‑128 (MQ=255)

                                 cTAAATTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:1466906/1‑128 (MQ=255)

                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           >  1:2988399/1‑122 (MQ=255)

                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  1:1829913/1‑128 (MQ=255)

                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  1:3423758/1‑128 (MQ=255)

                                 cTAAAATTATTTTAACATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  1:1051076/1‑128 (MQ=255)

                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  1:265257/128‑1 (MQ=255)

                                  tAAAATTATTATATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  1:3947039/128‑1 (MQ=255)

                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                   >  1:1220284/1‑128 (MQ=255)

                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                   <  1:1605029/128‑1 (MQ=255)

                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                   <  1:922324/128‑1 (MQ=255)

                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                  <  2:1874934/128‑1 (MQ=255)

                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                 >  2:2572857/1‑128 (MQ=255)

                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTATAATTTTAATAACTGACTTATTTTTCTAATTTTTAAATTATTTTCaaa                                                                                 >  2:2127675/1‑128 (MQ=255)

                                        tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                              <  2:3169059/128‑1 (MQ=255)

                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          <  1:1888024/128‑1 (MQ=255)

                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          >  1:1275593/1‑128 (MQ=255)

                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                             <  1:2195876/124‑1 (MQ=255)

                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                         <  2:3644926/128‑1 (MQ=255)

                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                         <  1:3884092/128‑1 (MQ=255)

                                             aaTCATTT‑AAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                        <  2:3465350/127‑1 (MQ=255)

                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                      >  2:2817234/1‑128 (MQ=255)

                                                 aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                     >  1:960140/1‑128 (MQ=255)

                                                  tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                    >  1:2348917/1‑128 (MQ=255)

                                                      aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACCGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                >  2:759870/1‑128 (MQ=255)

                                                         aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAATAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                             <  1:2028712/128‑1 (MQ=255)

                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  2:2694190/88‑1 (MQ=255)

                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCTAATCATTATCTATTAATCAAtttt                                                           >  2:1282704/1‑128 (MQ=255)

                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           <  2:2033850/75‑1 (MQ=255)

                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTCTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑Attat                                                     >  1:755874/1‑125 (MQ=255)

                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTGTTAAATTAATTTCAAATCATTATCTATTAATCAATTTT‑ATTCt                                                     >  1:2583502/1‑128 (MQ=255)

                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     <  1:228345/95‑1 (MQ=255)

                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTATTCAATTTT‑ATGCTCg                                                   >  2:1193768/1‑128 (MQ=255)

                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCg                                                   >  2:237327/1‑128 (MQ=255)

                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTCAAATAATTATCTATTAATAAATTTT‑ATgatatt                                                  >  1:1963983/1‑123 (MQ=255)

                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑t                                                >  2:1015671/1‑128 (MQ=255)

                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑t                                                >  2:3751419/1‑128 (MQ=255)

                                                                    ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑tt                                               >  2:3536768/1‑128 (MQ=255)

                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATTCTCG‑‑tta                                              >  1:2896097/1‑127 (MQ=255)

                                                                        tattttatatatTATAAAACTAATATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCGCG‑‑tttttt                                           <  1:525063/122‑1 (MQ=255)

                                                                        tatttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑tttttt                                           <  1:878955/125‑1 (MQ=255)

                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                           >  1:2720450/1‑107 (MQ=255)

                                                                                  atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑tttttttttttttttt                                 >  1:1681835/1‑128 (MQ=255)

                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAATTTT‑ATGCTCt‑‑tttttttttttttttttta                              >  2:2305433/1‑126 (MQ=255)

                                                                                       aaaaCTACTATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTAATTTAAAATCATTATCTAATAATAAATTTT‑ATTCTCG‑‑ttttttttttttttttttaca                            <  1:2250190/128‑5 (MQ=255)

                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑ttttttttttttttttttaaaa                           >  2:2754015/1‑123 (MQ=255)

                                                                                        aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTAT‑ATGATCT‑‑TTTTTTTTTTTTTTTTTAaaaa                           >  1:1514209/1‑128 (MQ=255)

                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTTTTaaaaaaa                       >  1:459516/1‑126 (MQ=255)

                                                                                            taatatcACTTTTTTATAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAata                       <  2:941839/122‑1 (MQ=255)

                                                                                             actaGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTTTTTTTAAAATTTTTAAATTATTTTTAAATCATTATATATTAATCAATTTT‑ATGCTCG‑‑ttttttttttttttttttttacaaaaa                      <  1:3146696/128‑11 (MQ=255)

                                                                                              ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCGT‑TTTTTTTTTTTTTTTTTAAAAAAatat                      >  1:3817035/1‑128 (MQ=255)

                                                                                               taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCGT‑TTTTTTTTTTTTTTTTTAAAAAAAtata                     >  1:1323138/1‑128 (MQ=255)

                                                                                               atcaaattttttAAAAATTTTTAAATATAAAAAATTTAA‑TATTTTTAAAATTTTTAAAATATTTTTAAAAAATTATATTTTAATCAATTTTAATGCCGG‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                    <  1:3048795/122‑1 (MQ=255)

                                                                                                tgaaattttttAAAATATTTTTAATTTTAATAACTGAATATTTTTTCAAATTTTTAAATTAATTTCAAATCATTATATATTAATCAAATTT‑ATGCTCGT‑TTTTTTTTTTTTTTTTTACAAAAATATaa                    <  1:3626042/123‑1 (MQ=255)

                                                                                                 ttaattttttAAAAAAATTTTAATTTTAATAAATGAATTATTTTTAAAATTGTTAAATTTTTTTCAAATCATTATCTATTAAACAATTTT‑ATGCGCGT‑TTTTTTTTTTTTTTTTTACAAAAATATAat                   <  1:3199076/124‑1 (MQ=255)

                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATTCTCG‑‑TTATTTTTTTTTTTTTTAaaaaaaattaaat                  >  1:2103355/1‑121 (MQ=255)

                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑ttttt                                            <  1:3692074/101‑1 (MQ=255)

                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCGTTTTTTTTTTTTTTTTTTTACAAAAATATAatt                  >  2:3273173/1‑128 (MQ=255)

                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCGT‑TTTTTTTTTTTTTTTTTACAAAAATATAtttt                 >  2:3318040/1‑128 (MQ=255)

                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTAACAAAAATAAAattta                >  2:3109570/1‑128 (MQ=255)

                                                                                                         ttAAAATATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTTTTTTCAAATAATTATCTTTTAATTAATTTT‑ATGGTGGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa           <  2:358601/128‑1 (MQ=255)

                                                                                                         ttAAAATATTTTTAATTTATATAAATTAATTATTTTTAAAATTTTAAAATTATTTTAAAATCATTATATATTAATAAATTTT‑TTGCTGGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa           <  1:347598/128‑1 (MQ=255)

                                                                                                         ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAAtttattttat          >  1:683617/1‑128 (MQ=255)

                                                                                                         taaaaaaaTATTAAATT‑TAATAAATTAATTTATTTTCAAATATTTAAATTATTTTATAATCTTTATCTTTTAATCAATTTT‑ATGCTCGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAt          <  2:3235455/126‑1 (MQ=255)

                                                                                                          tAAAAAATTTTTAATTTTAATTAATTATTTTTTTTTTAAATTTTTTATTTTTTTTAAAATAATTATTTATTTATAATTTTT‑TTTTTGT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt         <  1:2784886/128‑1 (MQ=255)

                                                                                                           aaaaaaTTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTTTTTTAAAATAATTATATATTAATAAATTTT‑TTTCTCGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt         <  1:1394141/128‑1 (MQ=255)

                                                                                                             aaaaTTTTAATTTTAAAAAATTAAATT‑TTTTTAAAAATTTTAAATTATTTTAAAATAAAAATTTATTAATAAATTTT‑ATTCTTGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa      <  2:3379922/128‑1 (MQ=255)

                                                                                                              aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑‑‑TTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGt   >  1:3311875/1‑128 (MQ=255)

||

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTT‑ATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420041‑420281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`