BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_021214	1,577,174	C→T	E979K (GAA→AAA)	TMARI_RS08125 ←	peptidase C11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_021214	1,577,174	0	C	T	95.7%	849.0 / 13.4	234	E979K (GAA→AAA)	TMARI_RS08125	peptidase C11
Reads supporting (aligned to +/- strand): ref base C (7/3); new base T (117/107); total (124/110)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 235 total aligned reads displayed.

TAGCTCAGGGGAAGAGCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTCTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAATATACTTACAGGAT  >  NC_021214/1576938‑1577413

tggcgcAGGGTAAGCGCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTC‑AAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTgg                                                                                                                                                                                                                                              <  1:673795/234‑1 (MQ=255)

    tCAGGGGAAGAGCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCTCCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGaa                                                                                                                                                                                                                                            <  2:960436/238‑1 (MQ=255)

         ggAAGGCCGCCTGCCTTACAAGCAGGAGGTGGTAGGTTCAATTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGAtct                                                                                                                                                                                                                                       <  1:231650/238‑1 (MQ=255)

         ggAAGAGCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCAGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGAtc                                                                                                                                                                                                                                        <  1:1193788/237‑1 (MQ=255)

            agagCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCCGTTTCCCCCCCGGCCCCCCGTTCTGTTCAAAAGATATTCAGCCC‑GGGCAAAAAAGCCGGGCGTTTTTTATTTTTCTTGGTTTTAATTTTTG‑TT‑ACTATTTTTGGAGATTTTGTa                                                                                                                                                                                                                                   >  1:892027/1‑239 (MQ=255)

               gcgcCTTCCTTACATGCAGGTGCTCTTAGGTTACAGTCCTGCCGCTCCCACCAGCGACGTCAGGTGGCCAGGTAGCTCAGTTTGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGTGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTAGTTTGGAAGATCTCGTAGCa                                                                                                                                                                                                                                <  1:1008096/239‑1 (MQ=255)

               gcgcCTGCCTTACAAGCAGGGGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCa                                                                                                                                                                                                                                <  1:13101/239‑1 (MQ=255)

                 gcctgcctTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCCATTCCCCCCCCGGCCACCAGTTCTGGTTAAAAGATATTCAGCCC‑GGTCCAAAAGGCCGGGGATTTTTTATTTTTT‑TGGGTTTAGATTTTG‑TTCACTATTTTTGAAGATTTCGGAGCaa                                                                                                                                                                                                                               >  1:652336/1‑238 (MQ=255)

                  cctgcctTACAATCGGGGGGTCGTAGTTTGAATTCCTGCCGCTCCCACCAGAGATGTCAGGGGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCt                                                                                                                                                                                                                            <  1:747655/240‑1 (MQ=255)

                     gcctTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCt                                                                                                                                                                                                                         <  2:233187/240‑1 (MQ=255)

                       ctTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTg                                                                                                                                                                                                                        <  2:210460/239‑1 (MQ=255)

                          ccAAGCAGGAGGTCTTGGGTTCAAGTCCGTCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTg                                                                                                                                                                                                                    <  1:45038/239‑1 (MQ=255)

                          aCAGGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTg                                                                                                                                                                                                                    <  2:492463/240‑1 (MQ=255)

                                aggaggTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGGTCTGTTCAAAAGATATTCAGCCC‑GGGCAAAAAGGCCCGGCTTTTTTTTTTTTTT‑TTGATTTAGATTTTG‑TTCACTATTTTTGAAGATCTCGTAACAACCTTCTGAGTGGTAGGa                                                                                                                                                                                                              >  2:644489/1‑240 (MQ=255)

                                 ggaggTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTTGATTCCGCCCCTGGCCACCAGGTCTGGTTAAAAGATATTCAGCCC‑GGGCAAAAAGGCCGGGCCTTTTGTATTATTT‑TGGATTTAGATTTTG‑TTCACTATTTTTGAAGATCTCGTAGCCACCTTTCGTGTGATTGGaa                                                                                                                                                                                                             >  1:1058909/1‑240 (MQ=255)

                                  ggggTCTTAGGTTCAAGGCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGaa                                                                                                                                                                                                             <  1:1022518/237‑1 (MQ=255)

                                       cGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGgag                                                                                                                                                                                                        <  2:167414/239‑1 (MQ=255)

                                                      tGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTTAGCCC‑GGGCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCCCCATTTTGGGAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTTCATTCCttttt                                                                                                                                                                                        >  2:259733/1‑240 (MQ=255)

                                                            gccccccAGGGATGTATGTTGGCCTGGTTGCTCAGTTGGTAGAACACTGGATTGAAAAGCCACGTGTCGGGGTGTCGAGTACGCCCCTAGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGACAAAAAGGCCGAGCTTTTTATATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCACCCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTag                                                                                                                                                                                  <  1:660621/239‑1 (MQ=255)

                                                                     agaTGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCCGGCCACCCGTTCTGTTTCAAAGATATTCAGCCC‑GGGCAAAAAAGCCGGGCGTTTTGTATTATTC‑TGGGTTTAGATTTTG‑TTTACTATTCTGGAAGATCTCGTAGGAAACTTCTTAGGGATAGGGAAGGAGATTTTAATTCCTTTTTTTTTAGAGAAttt                                                                                                                                                                           >  1:1171724/1‑237 (MQ=255)

                                                                      gaTGTCAGGAGGCCAGGTAGCTGAGTTGGTAGAGAACTGGCCTGAAAAGCCAGGTGTCGGCGGTGCGATTCCGCCCCTGGCCGCCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGc                                                                                                                                                                          <  1:1081249/238‑1 (MQ=255)

                                                                           cAGGTGGCCAGGTATCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCGGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGTGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGTAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGaa                                                                                                                                                                   <  1:393267/240‑1 (MQ=255)

                                                                           cAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGGTCTGTTCAAAAGATATTTAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTTTTT‑TGGATTTTGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGAATTCAATTCCTTTTTTTTTTGAGAATTTTCTAAGaa                                                                                                                                                                   >  2:1042696/1‑240 (MQ=255)

                                                                           cAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCCGGCCCCCAGTTCTGTTCAAAAGATATTTAGCCC‑GGGCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGGTTTAGATTTTG‑TTCACTCTTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGaa                                                                                                                                                                   >  2:541331/1‑240 (MQ=255)

                                                                                ggCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGGTTCCCCCCCCGGCCACCCGTTCTGTTTAAAAGATAATCAGCCCGGGCCAAAAAGGCC‑GGCTTTTTTTATTATTC‑TTGATTTTGCTTTTG‑TTCACTCTTTTGGAAGATCTCGCAGCAACCTTCTGAGGGATAGGAAAGGAGATTTTAATTCCTTTTTTTTTATAGAATTGCCTTAGAAAACAg                                                                                                                                                              >  1:756478/1‑240 (MQ=255)

                                                                                 gCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAg                                                                                                                                                              <  1:988659/239‑1 (MQ=255)

                                                                                  ccAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGt                                                                                                                                                             <  2:122834/239‑1 (MQ=255)

                                                                                     ggTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCTATTTCGCCCCCGGCCACCCGGTCTGTTCCAAAAGTATTCAGCCC‑GGTCCAAAAGGCCGGGCTTTTTGTATTTTTC‑TGGGTTTATATTTTG‑TTCCATATTTTTGAAGATCTCGTAGCAAACTTCTTAGTGATAGGGAAGGAAGTTTCAATTTCTTTTTTTTTATTGAATTGTCTAAAAAAACAGTTgt                                                                                                                                                          >  1:51072/1‑238 (MQ=255)

                                                                                        aGCTCAGTTGGCAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGTTTCTCCCCCCGGCCACCCGTTCTGTTCCAAAGCTATTTATCCC‑CGACAAAAAGCCCGGGCACTTTGTGTTATTT‑TCGGTTTTGATTTTGTTTCCCTTTTTTGGAA‑ATCTCGTAGCTACCCTATGGCTGGTTGGAAAGGGGAATTTCATTCCTTTTTATTTAGAGAATTGCGTAAGaaaatcagtgcgc                                                                                                                                                        >  1:1011706/1‑228 (MQ=255)

                                                                                         gCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGGTCTGTTCAAAAGATATTTAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTTATAGGAAAGGAGATTTTAATTCCTTTTTTTTTAGAGAATTGCCTAAGACAACAGTGTcccccg                                                                                                                                                     >  1:43411/1‑238 (MQ=255)

                                                                                              gTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTTGATTCCGCCCCCGGCCACCAGTTTTGTTCCAAAAATATTCAGCCC‑GGTCAAAAAAGCCGGGCTTTTTGTATTATTC‑TGGGTTTAGATTTTT‑TTCCCTCTTTTGGGAGATCTCGTAGCAAACTTTTGCGTGGTAGGGAAGGAGAGTTTCATTTCTTTTTCTTTTGAGAATTTGCTTAGAAAACAGTTGCCCCTGCTTTg                                                                                                                                                >  1:765464/1‑240 (MQ=255)

                                                                                                tGGTAGAGCCTTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCATTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGt                                                                                                                                               <  2:1051088/239‑1 (MQ=255)

                                                                                                tGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGt                                                                                                                                               <  2:68287/239‑1 (MQ=255)

                                                                                                tGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGt                                                                                                                                               <  2:828238/239‑1 (MQ=255)

                                                                                                         aCTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACaaa                                                                                                                                       <  2:89001/238‑1 (MQ=255)

                                                                                                            ggACTGAAAATCCAGGTGTCGGCGGTTCGATTCCCCCCCTGGCCACCAGTTCTGTTTAAAAGATATTTAGCCC‑GGTCCAAAAGGCCGGGCTTTTTGTGTTCTTT‑TGGATTTAAATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGGGATTTCAATTCCTTTTTTTTTTGAGAATTGCCTAAGAAAGAAGTTGCCCCTGATTTGTTGGCAAAAACGTc                                                                                                                                  >  2:872958/1‑240 (MQ=255)

                                                                                                                 gAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCCGGCCACCCGGTCTGTTCAAAAGATATTTAGCCC‑GGTCAAAAAAGCCGGGCTTTTTTTATTATTC‑TGGATTTAGATTTTT‑TTCACTATTTTGGAAGATCTCCTAGCAACCCTTTTTGTGTTAAGAAAAGAGATTTCAATTCCTTTTTCTTTTGAGAATTGCCTAAGAAAACAGTTTCCCCTGATTTGTAGGACAAAACGTCACCtt                                                                                                                             >  2:926860/1‑239 (MQ=255)

                                                                                                                    aaTCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAAGCCGGGCTTTTTGTATTATTC‑TGGATTTTGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTTAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGGTGCCCCTGATTTGTAGGACAAAAAGTCACCTGttc                                                                                                                          >  1:1208247/1‑238 (MQ=255)

                                                                                                                      tCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCCCCAGATCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGGTTTAGATTTTG‑TTCACTATTTTGGATGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTTTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAAAGTCACCTGTCTGa                                                                                                                        >  2:624019/1‑240 (MQ=255)

                                                                                                                      tCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTTCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTTTAGGACAAAACGTCACCTGTCTGa                                                                                                                        >  2:80846/1‑240 (MQ=255)

                                                                                                                            tgtCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCa                                                                                                                   <  1:31640/239‑1 (MQ=255)

                                                                                                                                          ttCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAAtt                                                                                                    <  2:421757/240‑1 (MQ=255)

                                                                                                                                              gCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGa                                                                                                 >  2:399757/1‑239 (MQ=255)

                                                                                                                                                  cTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACcaca                                                                                            >  1:255780/1‑240 (MQ=255)

                                                                                                                                                    ggCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAgg                                                                                          <  1:407908/240‑1 (MQ=255)

                                                                                                                                                        accaGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGAcc                                                                                               <  2:748703/231‑1 (MQ=255)

                                                                                                                                                         ccaGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGtt                                                                                      >  1:1122277/1‑239 (MQ=255)

                                                                                                                                                            gTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTg                                                                                  >  1:869659/1‑240 (MQ=255)

                                                                                                                                                              tCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTggg                                                                                <  1:157068/240‑1 (MQ=255)

                                                                                                                                                                tGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGgag                                                                              <  1:64241/240‑1 (MQ=255)

                                                                                                                                                                   tCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGaaa                                                                           <  2:316634/240‑1 (MQ=255)

                                                                                                                                                                   tCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGaaa                                                                           <  1:479065/240‑1 (MQ=255)

                                                                                                                                                                     aaaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaag                                                                         >  2:333172/1‑240 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaaga                                                                        >  2:1024942/1‑240 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaaga                                                                        >  2:279670/1‑240 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaaga                                                                        >  1:570620/1‑240 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaag                                                                         <  1:347004/239‑1 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaag                                                                         >  2:347004/1‑239 (MQ=255)

                                                                                                                                                                      aaaGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTCTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAaaga                                                                        <  2:1171724/240‑1 (MQ=255)

                                                                                                                                                                           tatTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGGGAAacgaaagga                                                                   >  1:924467/1‑236 (MQ=255)

                                                                                                                                                                           tatTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAagaaga                                                                     <  2:946839/238‑1 (MQ=255)

                                                                                                                                                                           tatTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAgaga                                                                   >  2:175218/1‑240 (MQ=255)

                                                                                                                                                                           tatTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACAACAGGAGTTCCTGGGAGAAAAGAAgaga                                                                   >  1:1107358/1‑240 (MQ=255)

                                                                                                                                                                             ttCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCTCCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAAt                                                                 <  2:43411/240‑1 (MQ=255)

                                                                                                                                                                                aGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGt                                                              >  1:581283/1‑240 (MQ=255)

                                                                                                                                                                                 gCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTa                                                             >  1:809884/1‑240 (MQ=255)

                                                                                                                                                                                  ccc‑GGTCAAAAAGGCCGGGATTTTTGTATTATTG‑TGGCTTTAGATTTTG‑TTAACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGACTTTCCTAAGCAAGCAGTTGGCCCTGATTTGTAGGACAAAACGTCACCTGTATGATACCAAGATTAAAGGAAATGGGAACACAGGAGTACCTGGGAGAAATGAAGAGAAATGTa                                                             >  1:937370/1‑239 (MQ=255)

                                                                                                                                                                                      ggTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCg                                                         <  2:476433/240‑1 (MQ=255)

                                                                                                                                                                                            aaaGGCCTGGCTTTTTGTAGTATTC‑TGGATTTAGATTTTG‑TTCACTAGTTTGGAAGATCGCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTTGACCACAGGAGTTCCTGGGAGAAAAGAAGTGAATTGTATGCGCTTccc                                                   <  1:621269/240‑1 (MQ=255)

                                                                                                                                                                                            aaaGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGCAGTCGCTTcc                                                    >  1:628532/1‑239 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCaa                                                <  2:897600/238‑1 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCaa                                                <  2:1121958/238‑1 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCaa                                                <  1:17514/238‑1 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCaa                                                <  2:51072/238‑1 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCaa                                                <  1:931091/238‑1 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATa                                              >  2:283537/1‑240 (MQ=255)

                                                                                                                                                                                                 ccGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATa                                              >  1:14627/1‑240 (MQ=255)

                                                                                                                                                                                                        ttttGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAgg                                       <  2:1138357/240‑1 (MQ=255)

                                                                                                                                                                                                        ttttGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAgg                                       <  2:756478/240‑1 (MQ=255)

                                                                                                                                                                                                         tttGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAggg                                      <  2:635176/240‑1 (MQ=255)

                                                                                                                                                                                                              attattC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGaa                                    >  2:593820/1‑237 (MQ=255)

                                                                                                                                                                                                                tattC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAGCCTTCTGAGTGATAGGaa                                                                                                                                                                                                             <  2:271440/66‑1 (MQ=255)

                                                                                                                                                                                                                tattC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTgg                               >  2:925657/1‑240 (MQ=255)

                                                                                                                                                                                                                  ttC‑TGGATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGAt                             <  2:581283/240‑1 (MQ=255)

                                                                                                                                                                                                                       ggATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGAt                                                                                                                                                                                                                  <  1:27979/55‑1 (MQ=255)

                                                                                                                                                                                                                       ggATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAACGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGCCCACAGGAGTTCCTGGGAGCAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGAtag                           >  1:1137132/1‑236 (MQ=255)

                                                                                                                                                                                                                       ggATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCg                           >  2:653089/1‑238 (MQ=255)

                                                                                                                                                                                                                       ggATTTAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCg                           >  2:1205753/1‑238 (MQ=255)

                                                                                                                                                                                                                           ttAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGc                     <  1:401079/240‑1 (MQ=255)

                                                                                                                                                                                                                            tAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAACACGTCACCTGTCTGATGCCAAGATTCAAGGAAGTTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGTAATCTGGATCGTCTGGCa                    >  1:835741/1‑240 (MQ=255)

                                                                                                                                                                                                                            tAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCa                    >  2:55903/1‑240 (MQ=255)

                                                                                                                                                                                                                            tAGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCa                    <  2:525469/240‑1 (MQ=255)

                                                                                                                                                                                                                             aGATTTTG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCAt                   <  1:541331/240‑1 (MQ=255)

                                                                                                                                                                                                                                 tttG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTATGGAATCTGGATCGTCTGGCATGAat               >  1:492576/1‑240 (MQ=255)

                                                                                                                                                                                                                                 tttG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAat               >  2:567181/1‑240 (MQ=255)

                                                                                                                                                                                                                                 tttG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAat               >  1:796289/1‑240 (MQ=255)

                                                                                                                                                                                                                                   tG‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAatat             <  1:154211/240‑1 (MQ=255)

                                                                                                                                                                                                                                    g‑TTCACTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGTGATTTCAATTCCTTTTTCTTTAGAGAATTGGCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAAtata            <  2:1075746/240‑1 (MQ=255)

                                                                                                                                                                                                                                         aCTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTg                                >  2:174088/1‑216 (MQ=255)

                                                                                                                                                                                                                                         aCTATTTTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAATATACTTa        <  2:130927/240‑1 (MQ=255)

TAGCTCAGGGGAAGAGCGCCTGCCTTACAAGCAGGAGGTCGTAGGTTCAAGTCCTGCCGCTCCCACCAGAGATGTCAGGTGGCCAGGTAGCTCAGTTGGTAGAGCACTGGACTGAAAATCCAGGTGTCGGCGGTTCGATTCCGCCCCTGGCCACCAGTTCTGTTCAAAAGATATTCAGCCC‑GGTCAAAAAGGCCGGGCTTTTTGTATTATTC‑TGGATTTAGATTTTG‑TTCACTATTCTGGAAGATCTCGTAGCAACCTTCTGAGTGATAGGAAAGGAGATTTCAATTCCTTTTTCTTTAGAGAATTGCCTAAGAAAGCAGTTGCCCCTGATTTGTAGGACAAAACGTCACCTGTCTGATGCCAAGATTCAAGGAAATTGGACCACAGGAGTTCCTGGGAGAAAAGAAGAGAATTGTAGTCGCTTCCCCAATAAATTAGGGAATCTGGATCGTCTGGCATGAATATACTTACAGGAT  >  NC_021214/1576938‑1577413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`