BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_021214	712,650	+A	coding (1199/1437 nt)	TMARI_RS03505 →	DNA polymerase III subunit gamma/tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_021214	712,650	1	.	A	98.7%	539.9 / ‑1.0	157	coding (1199/1437 nt)	TMARI_RS03505	DNA polymerase III subunit gamma/tau
Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (68/86); total (68/89)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 160 total aligned reads displayed.

TCGCCGAAGAAAAACGACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAG‑AAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑T‑CTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTGAGATTGTTTGAACAGGA  >  NC_021214/712413‑712881

tCGCCGAAGAAAAACGACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑t                                                                                                                                                                                                                                          >  1:1119590/1‑239 (MQ=255)

tCGCCGAAGAAAAACGACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑t                                                                                                                                                                                                                                          <  1:1184113/239‑1 (MQ=255)

tCGCCGAAGAAAAACGACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑t                                                                                                                                                                                                                                          <  1:620907/239‑1 (MQ=255)

               gACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAg                                                                                                                                                                                                                          <  2:232787/240‑1 (MQ=255)

                 cTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGc                                                                                                                                                                                                                         <  1:608196/239‑1 (MQ=255)

                 cTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGc                                                                                                                                                                                                                         <  1:443042/239‑1 (MQ=255)

                  tCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGAAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGcc                                                                                                                                                                                                                        >  2:779006/1‑239 (MQ=255)

                    gTCTGTAAATTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCctc                                                                                                                                                                                                                      <  2:241887/239‑1 (MQ=255)

                    gTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCctc                                                                                                                                                                                                                      >  1:241887/1‑239 (MQ=255)

                      cTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCctc                                                                                                                                                                                                                      <  1:722130/237‑1 (MQ=255)

                      cTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCctc                                                                                                                                                                                                                      >  2:722130/1‑237 (MQ=255)

                      cTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCa                                                                                                                                                                                                                   >  2:70400/1‑240 (MQ=255)

                        gTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCaga                                                                                                                                                                                                                 <  1:1052802/240‑1 (MQ=255)

                        gTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCaga                                                                                                                                                                                                                 <  2:1245380/240‑1 (MQ=255)

                             gTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGc                                                                                                                                                                                                            >  1:1054973/1‑240 (MQ=255)

                               gggTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAg                                                                                                                                                                                                          >  1:955150/1‑240 (MQ=255)

                               gggTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAAGTGCAg                                                                                                                                                                                                          >  2:830910/1‑240 (MQ=255)

                               gggTTCGGCTTACATAGAGACGAGGTTCTCCACCACAAACGTGCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAGCTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGc                                                                                                                                                                                                            <  2:625405/238‑1 (MQ=255)

                                   tCGGCTTACAGAGCTACGAAGTTCTCCACCACAAACGTGCAGGAAAGCGATGTCAGAGAAAAAAAACGATAAGTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAGACGCTTCAAAGAACTCAAGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGttt                                                                                                                                                                                                       <  2:1288325/239‑1 (MQ=255)

                                     ggCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGAc                                                                                                                                                                                                    <  1:648060/240‑1 (MQ=255)

                                            aTAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGaaa                                                                                                                                                                                             <  2:464604/240‑1 (MQ=255)

                                            aTAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGaaa                                                                                                                                                                                             <  1:414709/240‑1 (MQ=255)

                                            aTAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGaaa                                                                                                                                                                                             <  1:850610/240‑1 (MQ=255)

                                               gCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAgg                                                                                                                                                                                          >  1:1032048/1‑240 (MQ=255)

                                                 gacgaGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAgg                                                                                                                                                                                          <  1:298120/238‑1 (MQ=255)

                                                         tctcCACCACAAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTAttt                                                                                                                                                                                 <  2:753300/239‑1 (MQ=255)

                                                                cacaAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGAc                                                                                                                                                                                                    >  1:132200/1‑213 (MQ=255)

                                                                 acaAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGAtt                                                                                                                                                                        <  1:612702/240‑1 (MQ=255)

                                                                 acaAACGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGAt                                                                                                                                                                         >  1:1085576/1‑239 (MQ=255)

                                                                    aaCGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCa                                                                                                                                                                      >  1:132356/1‑239 (MQ=255)

                                                                      cGTTCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCg                                                                                                                                                                   <  2:914757/240‑1 (MQ=255)

                                                                         tCAGGAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGaa                                                                                                                                                                 <  1:385295/239‑1 (MQ=255)

                                                                            ggAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCa                                                                                                                                                                                                                   >  2:1305358/1‑186 (MQ=255)

                                                                            ggAAAACGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCt                                                                                                                                                             >  1:782097/1‑240 (MQ=255)

                                                                              aaaaCGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGc                                                                                                                                                              <  1:242730/237‑1 (MQ=255)

                                                                              aaaaCGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGc                                                                                                                                                              <  2:1032048/237‑1 (MQ=255)

                                                                              aaaaCGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTa                                                                                                                                                            <  1:710820/239‑1 (MQ=255)

                                                                               aaaCGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTAt                                                                                                                                                           <  1:33597/239‑1 (MQ=255)

                                                                                 aCGATGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCa                                                                                                                                                        <  1:1170961/240‑1 (MQ=255)

                                                                                    aTGTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCAtt                                                                                                                                                      >  2:1307604/1‑239 (MQ=255)

                                                                                      gTCAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAgg                                                                                                                                                                                          >  1:1163461/1‑201 (MQ=255)

                                                                                        cAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGa                                                                                                                                                  <  1:901393/239‑1 (MQ=255)

                                                                                        cAGAGAAAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGa                                                                                                                                                  <  2:1143145/239‑1 (MQ=255)

                                                                                        aagagaaaaaaaaCGATAATTCAAAAGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACg                                                                                                                                                   <  2:1218272/232‑1 (MQ=255)

                                                                                          gagaaaaaaaaCGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGtt                                                                                                                                               >  1:598542/5‑240 (MQ=255)

                                                                                              aaaaaaaCGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTtgatg                                                                                                                                           <  1:847436/240‑1 (MQ=255)

                                                                                                    aCGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGaaa                                                                                                                                     <  1:1307604/240‑1 (MQ=255)

                                                                                                        tAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGaaaaaa                                                                                                                                  <  1:779006/239‑1 (MQ=255)

                                                                                                               aaTGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGa                                                                                                                          <  1:858699/240‑1 (MQ=255)

                                                                                                                 tGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGc                                                                                                                        <  1:1062883/240‑1 (MQ=255)

                                                                                                                       gcagAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTtgatg                                                                                                                                           <  2:1080579/215‑1 (MQ=255)

                                                                                                                       gcagAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGaaa                                                                                                                  >  1:371827/1‑240 (MQ=255)

                                                                                                                         agaaagaaGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAACGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGAtt                                                                                                                                                                        >  1:197815/1‑184 (MQ=255)

                                                                                                                         agaaagaaGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAAc                                                                                                                >  1:484852/1‑240 (MQ=255)

                                                                                                                            aagaagAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACAtt                                                                                                             >  2:851387/1‑240 (MQ=255)

                                                                                                                                 gagaagaAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGc                                                                                                                                                                                                                         >  1:542194/1‑127 (MQ=255)

                                                                                                                                 gagaagaAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGc                                                                                                                                                                                                                         <  2:542194/127‑1 (MQ=255)

                                                                                                                                 gagaagaAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACAttttt                                                                                                          >  2:628752/1‑238 (MQ=255)

                                                                                                                                 gagaagaAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACAttttt                                                                                                          >  2:497132/1‑238 (MQ=255)

                                                                                                                                   gaagaaAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTAt                                                                                                                                                           <  2:1280662/187‑1 (MQ=255)

                                                                                                                                             aCGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCTTGAGCTGGAAAACATTTTTTCTAGAAAACTCgg                                                                                            >  2:792067/1‑240 (MQ=255)

                                                                                                                                              cGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCggg                                                                                           <  1:561296/240‑1 (MQ=255)

                                                                                                                                                gTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGaa                                                                                         >  2:919589/1‑240 (MQ=255)

                                                                                                                                                gTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGaa                                                                                         >  1:699562/1‑240 (MQ=255)

                                                                                                                                                 tGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGaaa                                                                                        >  1:1315238/1‑240 (MQ=255)

                                                                                                                                                   aaGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGaaaa                                                                                       <  1:590457/239‑1 (MQ=255)

                                                                                                                                                    aGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGattatt                                                                                                                                                                                  >  2:462889/1‑147 (MQ=255)

                                                                                                                                                    aGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGattatt                                                                                                                                                                                  <  1:462889/147‑1 (MQ=255)

                                                                                                                                                    aGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGaaaaa                                                                                      <  2:321198/239‑1 (MQ=255)

                                                                                                                                                        aaaaGAATAAAGACAGGAAGTCATCGTGGTCGAGAACGTCTTCAAAGAACTCATGGAAGATCTTAAAGAAAAGGGCGATCTCTCTA‑TACTGGGTCGGTCGCATCCTCTCAGAGGTGCAGTTTGTTGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACGCTGTAAAAAAGt                                                                                   <  2:1101705/238‑1 (MQ=255)

                                                                                                                                                        aaaaGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTa                                                                                  <  1:577408/239‑1 (MQ=255)

                                                                                                                                                        aaaaGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCGCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTa                                                                                  <  2:598542/239‑1 (MQ=255)

                                                                                                                                                        aaaaGAAGAAAAACAAGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAg                                                                                 <  2:925286/240‑1 (MQ=255)

                                                                                                                                                             aagaaAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGtt                                                                            <  1:997767/240‑1 (MQ=255)

                                                                                                                                                                               gCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGa                                                          >  1:128291/1‑240 (MQ=255)

                                                                                                                                                                                 gAGTTCGAGAAACGCTTCAAAGAGCGCATGGAATAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAACCGCTCAGAGGTACAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCGGGACAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAGCGTCGACTGATAGGaa                                                         <  2:484852/239‑1 (MQ=255)

                                                                                                                                                                                 gAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGTGCGATCGCTCTA‑TCCGTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACAGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGGTGAACGTCGACTTATg                                                             <  2:294373/235‑1 (MQ=255)

                                                                                                                                                                                 gAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGaa                                                         <  2:371827/239‑1 (MQ=255)

                                                                                                                                                                                            aCGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACaa                                             <  1:830910/240‑1 (MQ=255)

                                                                                                                                                                                              gCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑T‑CTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCg                                          <  1:129309/240‑1 (MQ=255)

                                                                                                                                                                                                       aaCTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGaa                                                                                                                                                                 <  2:118210/113‑1 (MQ=255)

                                                                                                                                                                                                        aCTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGtt                                  >  1:649674/1‑239 (MQ=255)

                                                                                                                                                                                                          tCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTtct                               <  1:855436/240‑1 (MQ=255)

                                                                                                                                                                                                           cATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTtctc                              >  1:1017487/1‑240 (MQ=255)

                                                                                                                                                                                                             tGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCag                            >  2:196926/1‑240 (MQ=255)

                                                                                                                                                                                                                 agaaCTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCagaag                         <  1:497132/239‑1 (MQ=255)

                                                                                                                                                                                                                  gaaCTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACa                                              <  2:1158372/217‑1 (MQ=255)

                                                                                                                                                                                                                   aaCTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGAt                       >  2:603974/1‑239 (MQ=255)

                                                                                                                                                                                                                    aCTGAAAGAAAAGTGCGATCTCTGTA‑TACGTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACGGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGAt                       <  2:142765/238‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGAAAGAAAAGGGCGATCTCTCTATT‑CTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATcc                     <  1:961528/240‑1 (MQ=255)

                                                                                                                                                                                                                    aCTGAAAGAAAAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGAt                       <  2:886139/238‑1 (MQ=255)

                                                                                                                                                                                                                       gaaagaaaAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAg                                     >  1:124202/1‑221 (MQ=255)

                                                                                                                                                                                                                       gaaagaaaAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTga                  >  1:1269624/1‑240 (MQ=255)

                                                                                                                                                                                                                       gaaagaaaAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTga                  >  1:155927/1‑240 (MQ=255)

                                                                                                                                                                                                                         aagaaaAGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTgaga                <  2:155927/240‑1 (MQ=255)

                                                                                                                                                                                                                              aaGGGCGATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTGAGATTGtt           <  1:622028/240‑1 (MQ=255)

                                                                                                                                                                                                                                    gATCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTAAGAAGATCCTGAGATTGTTTGAACa     >  2:112518/1‑240 (MQ=255)

                                                                                                                                                                                                                                     aTCTCTCTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCg                                          >  1:187218/1‑202 (MQ=255)

                                                                                                                                                                                                                                      tctctcTA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTGAGATTGTTTGAACagg   <  1:1283888/240‑1 (MQ=255)

                                                                                                                                                                                                                                       ctctctA‑TACTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTGAGATTGTTTGAACagga  <  1:932691/240‑1 (MQ=255)

TCGCCGAAGAAAAACGACTCGTCTGTAAAGTGGGTTCGGCTTACATAGCGACGAGGTTCTCCACCACAAACGTTCAGGAAAACGATGTCAGAG‑AAAAAAACGATAATTCAAATGTACAGCAGAAAGAAGAGAAGAAAGAAACGGTGAAGGCAAAAGAAGAAAAACAGGAAGACAGCGAGTTCGAGAAACGCTTCAAAGAACTCATGGAAGAACTGAAAGAAAAGGGCGATCTCTCTA‑T‑CTTTGTCGCTCTCAGCCTCTCAGAGGTGCAGTTTGACGGAGAAAAGGTGATTATTTCTTTTGATTCATCGAAAGCTATGCATTACGAGTTGATGAAGAAAAAACTGCCTGAGCTGGAAAACATTTTTTCTAGAAAACTCGGGAAAAAAGTAGAAGTTGAACTTCGACTGATGGGAAAAGAAGAAACAATCGAGAAGGTTTCTCAGAAGATCCTGAGATTGTTTGAACAGGA  >  NC_021214/712413‑712881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`