breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,875,5210T→C23.0% 28.8 ‑9.2 ‑0.075A28A (GCT→GCCyidXputative lipoprotein
*NC_0009133,875,5220C→G22.7% 26.1 ‑10.1 0.075L29V (CTG→GTG) yidXputative lipoprotein
*NC_0009133,875,5240G→C14.5% 19.3 ‑6.1 0.077L29L (CTG→CTCyidXputative lipoprotein
*NC_0009133,875,5290C→G12.0% 14.9 ‑5.6 0.075S31C (TCC→TGC) yidXputative lipoprotein
*NC_0009133,875,5320A→T19.5% 12.8 ‑9.8 ‑0.077H32L (CAT→CTT) yidXputative lipoprotein
*NC_0009133,875,5250G→T18.2% 10.0 ‑8.4 ‑0.077V30F (GTT→TTT) yidXputative lipoprotein
*NC_0009134,476,9870T→.100.0% 4.1 1coding (1551/1815 nt)yjgLSopA‑central‑domain‑like hexapeptide repeat protein
*NC_0009134,476,9880T→.100.0% 4.1 1coding (1552/1815 nt)yjgLSopA‑central‑domain‑like hexapeptide repeat protein
*NC_000913237,2540A→C100.0% 1.5 1intergenic (+247/‑81)aspV/yafTtRNA‑Asp/lipoprotein
*NC_0009134,037,2860C→A100.0% 1.3 1noncoding (27/76 nt)alaTtRNA‑Ala
*NC_0009133,581,7730G→A100.0% 1.1 2intergenic (+147/‑90)yhhY/yhhZaminoacyl nucleotide detoxifying acetyltransferase/putative Hcp1 family polymorphic toxin protein; putative colicin‑like DNase/tRNase activity
*NC_0009131,633,3410T→G100.0% 0.9 1intergenic (+36/‑281)ydfK/pinQcold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
*NC_000913237,2560T→G100.0% 0.7 1intergenic (+249/‑79)aspV/yafTtRNA‑Asp/lipoprotein
*NC_0009131,197,7690A→C100.0% 0.7 1F157V (TTT→GTT) ymfEe14 prophage; putative inner membrane protein
*NC_0009131,300,6740C→A100.0% 0.7 1intergenic (+1429/‑508)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*NC_0009131,300,6760C→A100.0% 0.7 1intergenic (+1431/‑506)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*NC_0009131,633,3530G→A100.0% 0.7 1intergenic (+48/‑269)ydfK/pinQcold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
*NC_0009131,633,3570C→A100.0% 0.7 1intergenic (+52/‑265)ydfK/pinQcold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
*NC_0009131,633,3650C→A100.0% 0.7 1intergenic (+60/‑257)ydfK/pinQcold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
*NC_0009131,633,3710C→A100.0% 0.7 1intergenic (+66/‑251)ydfK/pinQcold shock protein, function unknown, Qin prophage/Qin prophage; putative site‑specific recombinase
*NC_0009131,806,2650A→C100.0% 0.6 1intergenic (‑182/‑105)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
*NC_0009132,884,2930T→A100.0% 0.2 2intergenic (‑155/+260)casA/ygcBCRISP RNA (crRNA) containing Cascade antiviral complex protein/Cascade complex anti‑viral R‑loop helicase‑annealase Cas3
*NC_0009132,726,5970C→A100.0% ‑0.5 1noncoding (2588/2904 nt)rrlG23S ribosomal RNA of rrnG operon
*NC_0009132,726,5980T→A100.0% ‑0.5 1noncoding (2587/2904 nt)rrlG23S ribosomal RNA of rrnG operon
*NC_0009132,726,6020C→A100.0% ‑0.5 1noncoding (2583/2904 nt)rrlG23S ribosomal RNA of rrnG operon
*NC_0009132,726,6030C→A100.0% ‑0.5 1noncoding (2582/2904 nt)rrlG23S ribosomal RNA of rrnG operon
*NC_0009132,726,6040C→A100.0% ‑0.5 1noncoding (2581/2904 nt)rrlG23S ribosomal RNA of rrnG operon

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009132,823,8300A→G24.7% 50.6 ‑0.2 ‑0.185intergenic (‑61/+19)recA/pncCDNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase
*NC_0009134,296,0600C→T22.9% 64.3 0.0 ‑0.0110intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2290113NA (NA)20 (0.250) 18/310 4.1 58.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2462930 = 14 (0.170)intergenic (+284/‑82) fadL/yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
* ? NC_000913 = 12994980 (0.000)19 (0.240) 18/302 4.0 97.4% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 1 (0.010)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1207790 =55 (0.680)14 (0.190) 10/278 5.9 22.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 50 (0.690)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 19785020 (0.000)12 (0.160) 10/294 6.2 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 120780558 (0.720)7 (0.100) 7/278 7.2 12.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 49 (0.680)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3619878 =NA (NA)6 (0.080) 5/292 8.5 100% coding (687/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619892 = 0 (0.000)coding (701/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 112281 =90 (1.120)5 (0.060) 4/298 9.3 5.8% coding (319/744 nt) zapD FtsZ stabilizer
?NC_000913 112312 = 77 (1.000)coding (288/744 nt) zapD FtsZ stabilizer
* ? NC_000913 18671 =89 (1.110)4 (0.050) 4/296 9.3 4.6% intergenic (+16/‑44) nhaA/nhaR sodium‑proton antiporter/transcriptional activator of nhaA
?NC_000913 18691 = 82 (1.070)intergenic (+36/‑24) nhaA/nhaR sodium‑proton antiporter/transcriptional activator of nhaA
* ? NC_000913 909017 =68 (0.840)4 (0.050) 4/296 9.3 5.7% coding (278/1002 nt) ltaE L‑allo‑threonine aldolase, PLP‑dependent
?NC_000913 909055 = 68 (0.880)coding (240/1002 nt) ltaE L‑allo‑threonine aldolase, PLP‑dependent
* ? NC_000913 = 3699911101 (1.250)4 (0.050) 4/292 9.2 4.0% intergenic (+18/+69) bcsG/ldrD DUF3260 family cellulose production inner membrane protein/toxic polypeptide, small
?NC_000913 = 3699921 98 (1.290)intergenic (+28/+59) bcsG/ldrD DUF3260 family cellulose production inner membrane protein/toxic polypeptide, small
* ? NC_000913 = 271382983 (1.030)4 (0.050) 3/288 9.8 5.0% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713908 76 (1.020)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 = 385820586 (1.070)4 (0.050) 3/284 9.7 4.7% coding (200/1494 nt) yidJ sulfatase/phosphatase superfamily protein
?NC_000913 = 3858228 83 (1.130)coding (177/1494 nt) yidJ sulfatase/phosphatase superfamily protein
* ? NC_000913 = 223651981 (1.010)3 (0.040) 3/290 9.8 4.0% intergenic (+19/+224) preA/mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
?NC_000913 = 2236533 68 (0.900)intergenic (+33/+210) preA/mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
* ? NC_000913 = 2291160128 (1.590)3 (0.040) 3/292 9.9 2.4% noncoding (8/199 nt) RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site
?NC_000913 = 2291167 119 (1.570)noncoding (15/199 nt) RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site
* ? NC_000913 2423437 =177 (2.200)3 (0.040) 3/294 9.9 1.8% coding (103/891 nt) yfcI transposase_31 family protein
?NC_000913 2423471 = 162 (2.120)coding (69/891 nt) yfcI transposase_31 family protein
* ? NC_000913 2540366 =84 (1.040)3 (0.040) 3/300 10.0 3.7% coding (449/1098 nt) cysA sulfate/thiosulfate transporter subunit
?NC_000913 2540387 = 74 (0.950)coding (428/1098 nt) cysA sulfate/thiosulfate transporter subunit
* ? NC_000913 2777562 =63 (0.780)3 (0.040) 3/292 9.9 4.8% coding (110/330 nt) ypjF CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
?NC_000913 2777586 = 60 (0.790)coding (134/330 nt) ypjF CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
* ? NC_000913 3515982 =59 (0.730)3 (0.040) 3/292 9.9 5.2% intergenic (‑69/+38) dam/damX DNA adenine methyltransferase/cell division protein that binds to the septal ring
?NC_000913 3516010 = 53 (0.700)intergenic (‑97/+10) dam/damX DNA adenine methyltransferase/cell division protein that binds to the septal ring
* ? NC_000913 3989869 =80 (0.990)3 (0.040) 3/294 9.9 3.6% coding (898/942 nt) hemC hydroxymethylbilane synthase
?NC_000913 3989977 = 87 (1.140)coding (790/942 nt) hemC hydroxymethylbilane synthase